scholarly journals Variation of total amount of phenolic compounds, radical scavenging activity and volatile com pounds of Liriodendron tulipifera L. and Ginkgo biloba L. leaves extracts during different vegetation periods

Biologija ◽  
2013 ◽  
Vol 59 (2) ◽  
Author(s):  
Jurgita Mikašauskaitė ◽  
Ona Ragažinskienė ◽  
Audrius Maruška
2017 ◽  
Vol 63 (1) ◽  
Author(s):  
Anna Nowak

Wstęp: Miłorząb japoński (Ginkgo biloba L.) jest uważany za cenną roślinę wykazującą działanie przeciwrodnikowe. Związane jest to m.in. z zawartością takich substancji aktywnych, jak polifenole, terpenoidy i witaminy. W celach leczniczych wykorzystuje się ekstrakty z liści, które stosowane są m.in. w profilaktyce chorób cywilizacyjnych. Celem badań było określenie aktywności antyoksydacyjnej wyciągów alkoholowych z suszonych liści miłorzębu japońskiego, opadłych z drzew po zakończonej wegetacji, które najczęściej są materiałem odpadowym.Materiały i metody: Surowiec roślinny stanowiły świeżo zebrane oraz suszone liście miłorzębu japońskiego. Ekstrakty do analiz przygotowano z wykorzystaniem aparatu Soxhleta, wytrząsarki rotacyjnej oraz łaźni ultradźwiękowej. Jako rozpuszczalnik zastosowano etanol oraz metanol. Zdolność do eliminowania wolnych rodników (radical scavenging activity – RSA) oznaczono metodą z użyciem odczynnika DPPH (2,2-difenylo-1-pikrylohydrazyl), natomiast całkowitą zawartość polifenoli metodą Folina–Ciocalteu’a.Wyniki: Aktywność antyoksydacyjna wyciągów z suchych liści miłorzębu mieściła się w granicach od 37,69 ±1,97 do 92,85 ±0,28% RSA, przy czym najwyższy wynik uzyskano w grupie ekstraktów w 40% (v/v) etanolu. Spośród wyciągów w 70% (v/v) etanolu najwyższymi wartościami RSA% charakteryzowały się liście ekstrahowane za pomocą wytrząsarki (92,37 ±0,84% RSA), natomiast w przypadku zastosowania 96% (v/v) etanolu – ekstrakty pochodzące z aparatu Soxhleta (81,71 ±2,10%). Duże zdolności przeciwutleniające wykazywały również wyciągi metanolowe. Do ich otrzymania wykorzystano wytrząsarkę i uzyskano wynik wynoszący 89,25 ±0,54% RSA. Najwyższą ogólną zawartość polifenoli odnotowano w próbach ekstrahowanych w aparacie Soxhleta (etanol 40%), w których zawartość wynosiła 9,15 mg/g surowca. Wykazano również dodatnią korelację pomiędzy ogólną zawartością polifenoli a aktywnością antyoksy­dacyjną wyrażoną w % RSA analizowanych prób.Wnioski: Wyciągi z żółtych liści miłorzębu japońskiego zebranych po zakończonej wegetacji charakteryzują się dużą zdolnością zmiatania wolnych rodników, co sugeruje możliwość wykorzystania tego materiału odpadowego jako cennego źródła antyoksydantów.


2014 ◽  
Vol 59 (3) ◽  
pp. 282-292 ◽  
Author(s):  
Ki Yuol Jung ◽  
Jee Yeon Ko ◽  
Jae Saeng Lee ◽  
Mi Sun Jeong ◽  
In Seok Oh ◽  
...  

2003 ◽  
Vol 128 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Md. Shahidul Islam ◽  
Makoto Yoshimoto ◽  
Koji Ishiguro ◽  
Shigenori Okuno ◽  
Osamu Yamakawa

The phenolic content and the radical scavenging activity were compared in leaves of sweetpotato (Ipomoea batatas L.) cultivars Shimon-1, Kyushu-119 and Elegant Summer grown under different temperature and shading conditions. Compared to cultivar differences, there was less effect of temperature and shading on the total phenolic content in sweetpotato leaves, however certain polyphenolic components differed widely among the treatments. The positive correlation between the radical scavenging activity and the level of total phenolics (r = 0.62) suggests that phenolic compounds are important antioxidant components of sweetpotato leaves. All the reverse-phase high-performance liquid chromatography (RP-HPLC) profiles of the cultivars tested showed peaks at the same retention times but peak areas of individual phenolic compounds differed with respective temperature and shading treatments. The phenolic compounds identified in the sweetpotato leaf were caffeic acid, chlorogenic acid, 4,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. Most of the phenolic compounds were highest in leaves from plants grown at 20 °C without shading except 4,5-di-O-caffeoylquinic acid. The results indicate that growing leaves under moderately high temperatures and in full sun enhances the accumulation of phenolic components. These phenolic components have possible value in enhancing human health.


Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 299 ◽  
Author(s):  
Urszula Szymanowska ◽  
Barbara Baraniak

Raspberry pomace was obtained from raspberries subjected to enzymatic maceration using three commercial pectinolytic preparations (Pectinex Ultra SP-L, Pectinex Yield Mash, and Ultrazym AFP-L). Phenolic compounds were extracted and anthocyanin fractions were isolated using the SPE solid phase extraction technique. In the separated anthocyanin fractions, the content of individual compounds was determined by the HPLC technique and the antioxidant activity was assessed with four complementary methods (DPPH and ABTS radical scavenging activity, chelating Fe(II) power, and ferric reducing power). Potential anti-inflammatory properties were also identified as the ability to inhibit the activity of lipoxygenase and cyclooxygenase 2. For these enzymes, the type of inhibition was determined based on the Lineweaver–Burke plot.


1995 ◽  
Vol 49 (11) ◽  
pp. 1649-1655 ◽  
Author(s):  
Indrani Maitra ◽  
Lucia Marcocci ◽  
Marie Therese Droy-Lefaix ◽  
Lester Packer

2020 ◽  
Vol 10 (4) ◽  
pp. 70-78
Author(s):  
Abir Rezzagui ◽  
Abderrahmane Senator ◽  
Soumia Benbrinis ◽  
Hamama Bouriche

This study was undertaken to evaluate the antioxidant and anti-hemolytic properties of Algerian Drimia maritima Baker flower extracts. Determination of phenolic content was carried out to estimate the chemical composition of D. maritima extracts. Antioxidant properties were investigated in all extracts using free radical scavenging activity (against DPPH, ABTS, hydroxyl radical, and superoxide anion), reducing power, inhibition of lipid peroxidation, and anti-hemolytic capacity. Phenolic determination revealed that D. maritima flowers contain phenolic compounds, flavonoids, and tannins. Ethyl acetate extract showed the highest reducing power and scavenging activity using DPPH and ABTS assays. However, aqueous extract was the most effective against hydroxyl radical, superoxide anion, and lipid peroxidation. The half-time of hemolysis indicates that chloroform extract exhibited the best anti-hemolytic capacity in the AAPH induced hemolysis model. The results of this study suggest that D. maritima could be used as a possible source of antioxidant phenolic compounds and that further determination of these compounds may provide more information on their medicinal value.  Keywords: Drimia maritima, phenolic compounds, scavenging activity, reducing power, anti-hemolytic.


Author(s):  
Fadime Seyrekoğlu ◽  
Hasan Temiz

H. perforatum, which is widely used in traditional medicine due to its bioactive compounds was extracted with ethanol-water (3:7). The extract was encapsulated with maltodextrin and gum arabic in a spray dryer in order to protect the phenolic compounds in its structure. Different amounts of microcapsules were added to our traditional drink, i.e. ayran (drinking yoghurt). The total phenolic content and DPPH radical scavenging activity of the microcapsules, extract of HP, and ayran samples were determined. The amount of total phenolic compounds in the microcapsule provided a superior effect than the extract. The ayran samples were supplemented with 2%, 3%, 4%, 5% and 6% of H. perforatum (HP) microcapsules and it is observed that total phenolic content (TPC) and DPPH radical scavenging activity indicated an increase with concentration. TPC and DPPH activity were determined as 256.94 mg GAE / 100mL and 78.05% for 6% of HP microcapsules supplemented samples. As a result of the sensory analysis, ayran samples which supplemented with 4% of HS microcapsule gained the highest scores by the panellists and received more appreciation than the control group. According to sensory analysis, HP4 (ayran produced with 4% HP added microcapsule) sample was determined as the best sample, while the HP6 (Ayran produced with 6% HP added microcapsule) sample had the highest scores in terms of DPPH scavenging activity and TPC results. The overall results of this study revealed that 4% HP supplemented ayran can be produced with its increased health benefits and desirable properties. In this study, the use of H. perforatum microcapsules in ayran, its effect on antioxidant and phenolic components, the usage rates and acceptability of microcapsules were investigated.


2013 ◽  
Vol 92 (5) ◽  
pp. 810-815 ◽  
Author(s):  
Pramote Khuwijitjaru ◽  
Jiraporn Plernjit ◽  
Boonyanuch Suaylam ◽  
Suched Samuhaseneetoo ◽  
Rungnaphar Pongsawatmanit ◽  
...  

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