Rapid Development of De Novo Thoracic Aneurysm After Liver Transplant

Autism spectrum disorder (ASD) cases have increased rapidly in recent decades, which is associated with various genetic abnormalities. To provide a better understanding of the genetic factors in ASD, we assessed the global scientific output of the related studies. A total of 2944 studies published between 1997 and 2018 were included by systematic retrieval from the Web of Science (WoS) database, whose scientific landscapes were drawn and the tendencies and research frontiers were explored through bibliometric methods. The United States has been acting as a leading explorer of the field worldwide in recent years. The rapid development of high-throughput technologies and bioinformatics transferred the research method from the traditional classic method to a big data-based pipeline. As a consequence, the focused research area and tendency were also changed, as the contribution of de novo mutations in ASD has been a research hotspot in the past several years and probably will remain one into the near future, which is consistent with the current opinions of the major etiology of ASD. Therefore, more attention and financial support should be paid to the deciphering of the de novo mutations in ASD. Meanwhile, the effective cooperation of multi-research centers and scientists in different fields should be advocated in the next step of scientific research undertaken.

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Calcineurin inhibitor-free immunosuppression with everolimus monotherapy in de novo liver transplant recipient: preliminary results

Digestive and Liver Disease ◽

10.1016/j.dld.2007.07.008 ◽

2007 ◽

Vol 39 (10) ◽

pp. A31-A32

Author(s):

M. Masetti ◽

R. Montalti ◽

A. Romano ◽

G. Rompianesi ◽

N. De Ruvo ◽

...

Keyword(s):

Transplant Recipient ◽

Liver Transplant ◽

Calcineurin Inhibitor ◽

Liver Transplant Recipient ◽

De Novo ◽

Preliminary Results

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Betel quid chewing leads to the development of unique de novo malignancies in liver transplant recipients, a retrospective single center study in Taiwan

Medicine ◽

10.1097/md.0000000000004901 ◽

2016 ◽

Vol 95 (37) ◽

pp. e4901 ◽

Cited By ~ 3

Author(s):

Yi-Chan Chen ◽

Chih-Hsien Cheng ◽

Yu-Chao Wang ◽

Ting-Jun Wu ◽

Hong-Shiue Chou ◽

...

Keyword(s):

Liver Transplant ◽

De Novo ◽

Betel Quid ◽

Transplant Recipients ◽

Single Center ◽

Single Center Study ◽

Betel Quid Chewing ◽

Liver Transplant Recipients ◽

Center Study

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A RANDOMIZED, OPEN-LABEL, COMPARATIVE, MULTI-CENTER STUDY TO ASSES THE SAFETY AND EFFICACY OF PROGRAF/MYFORTIC AND ADVAGRAF/MYFORTIC IN DE NOVO LIVER TRANSPLANT RECIPIENTS— A PRELIMINARY REPORT

Transplantation Journal ◽

10.1097/00007890-201007272-01225 ◽

2010 ◽

Vol 90 ◽

pp. 642

Author(s):

T. Chen ◽

H. Yang ◽

C. Hsieh ◽

S. Cheng ◽

Y. Lin ◽

...

Keyword(s):

Liver Transplant ◽

Preliminary Report ◽

De Novo ◽

Transplant Recipients ◽

Open Label ◽

Safety And Efficacy ◽

Multi Center Study ◽

Liver Transplant Recipients ◽

Center Study

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High-Throughput Quantitative Assay Technologies for Accelerating the Discovery and Optimization of Targeted Protein Degradation Therapeutics

SLAS DISCOVERY Advancing Life Sciences ◽

10.1177/2472555220985049 ◽

2021 ◽

pp. 247255522098504

Author(s):

Jeffrey R. Simard ◽

Linda Lee ◽

Ellen Vieux ◽

Reina Improgo ◽

Trang Tieu ◽

...

Keyword(s):

Drug Discovery ◽

Protein Degradation ◽

Protein Interactions ◽

Fluorescent Protein ◽

De Novo ◽

Rapid Development ◽

Dependent Manner ◽

Western Blots ◽

Advantages And Disadvantages ◽

Degradation Rates

The aberrant regulation of protein expression and function can drastically alter cellular physiology and lead to numerous pathophysiological conditions such as cancer, inflammatory diseases, and neurodegeneration. The steady-state expression levels of endogenous proteins are controlled by a balance of de novo synthesis rates and degradation rates. Moreover, the levels of activated proteins in signaling cascades can be further modulated by a variety of posttranslational modifications and protein–protein interactions. The field of targeted protein degradation is an emerging area for drug discovery in which small molecules are used to recruit E3 ubiquitin ligases to catalyze the ubiquitination and subsequent degradation of disease-causing target proteins by the proteasome in both a dose- and time-dependent manner. Traditional approaches for quantifying protein level changes in cells, such as Western blots, are typically low throughput with limited quantification, making it hard to drive the rapid development of therapeutics that induce selective, rapid, and sustained protein degradation. In the last decade, a number of techniques and technologies have emerged that have helped to accelerate targeted protein degradation drug discovery efforts, including the use of fluorescent protein fusions and reporter tags, flow cytometry, time-resolved fluorescence energy transfer (TR-FRET), and split luciferase systems. Here we discuss the advantages and disadvantages associated with these technologies and their application to the development and optimization of degraders as therapeutics.

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Quick and efficient approach to develop genomic resources in orphan species: Application in Lavandula angustifolia

PLoS ONE ◽

10.1371/journal.pone.0243853 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0243853

Author(s):

Berline Fopa Fomeju ◽

Dominique Brunel ◽

Aurélie Bérard ◽

Jean-Baptiste Rivoal ◽

Philippe Gallois ◽

...

Keyword(s):

Large Scale ◽

De Novo ◽

Rapid Development ◽

Genetic Distances ◽

Lavandula Angustifolia ◽

Distance Analysis ◽

Alternative Medicines ◽

Dna And Rna ◽

Snp Development ◽

High Level

Next-Generation Sequencing (NGS) technologies, by reducing the cost and increasing the throughput of sequencing, have opened doors to generate genomic data in a range of previously poorly studied species. In this study, we propose a method for the rapid development of a large-scale molecular resources for orphan species. We studied as an example the true lavender (Lavandula angustifolia Mill.), a perennial sub-shrub plant native from the Mediterranean region and whose essential oil have numerous applications in cosmetics, pharmaceuticals, and alternative medicines. The heterozygous clone “Maillette” was used as a reference for DNA and RNA sequencing. We first built a reference Unigene, compound of coding sequences, thanks to de novo RNA-seq assembly. Then, we reconstructed the complete genes sequences (with introns and exons) using an Unigene-guided DNA-seq assembly approach. This aimed to maximize the possibilities of finding polymorphism between genetically close individuals despite the lack of a reference genome. Finally, we used these resources for SNP mining within a collection of 16 commercial lavender clones and tested the SNP within the scope of a genetic distance analysis. We obtained a cleaned reference of 8, 030 functionally in silico annotated genes. We found 359K polymorphic sites and observed a high SNP frequency (mean of 1 SNP per 90 bp) and a high level of heterozygosity (more than 60% of heterozygous SNP per genotype). On overall, we found similar genetic distances between pairs of clones, which is probably related to the out-crossing nature of the species and the restricted area of cultivation. The proposed method is transferable to other orphan species, requires little bioinformatics resources and can be realized within a year. This is also the first reported large-scale SNP development on Lavandula angustifolia. All the genomics resources developed herein are publicly available and provide a rich pool of molecular resources to explore and exploit lavender genetic diversity in breeding programs.

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LeafGo: Leaf to Genome, a quick workflow to produce high-quality De novo genomes with Third Generation Sequencing technology

10.1101/2021.01.25.428044 ◽

2021 ◽

Author(s):

Patrick Driguez ◽

Salim Bougouffa ◽

Karen Carty ◽

Alexander Putra ◽

Kamel Jabbari ◽

...

Keyword(s):

De Novo ◽

Rapid Development ◽

Plant Genome ◽

Plant Genomics ◽

High Quality ◽

High Molecular Weight Dna ◽

Tissue Samples ◽

Sequencing Technologies ◽

The Cost ◽

New Generation

AbstractRecent years have witnessed a rapid development of sequencing technologies. Fundamental differences and limitations among various platforms impact the time, the cost and the accuracy for sequencing whole genomes. Here we designed a complete de novo plant genome generation workflow that starts from plant tissue samples and produces high-quality draft genomes with relatively modest laboratory and bioinformatic resources within seven days. To optimize our workflow we selected different species of plants which were used to extract high molecular weight DNA, to make PacBio and ONT libraries for sequencing with the Sequel I, Sequel II and GridION platforms. We assembled high-quality draft genomes of two different Eucalyptus species E. rudis, and E. camaldulensis to chromosome level without using additional scaffolding technologies. For the rapid production of de novo genome assembly of plant species we showed that our DNA extraction protocol followed by PacBio high fidelity sequencing, and assembly with new generation assemblers such as hifiasm produce excellent results. Our findings will be a valuable benchmark for groups planning wet- and dry-lab plant genomics research and for high throughput plant genomics initiatives.

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