scholarly journals Growth and organic solutes accumulation in seedlings of Caatinga tree species in response of water deficit and potassium

2021 ◽  
Vol 12 (2) ◽  
pp. 57-67
Author(s):  
Ediglécia Pereira de Almeida ◽  
Antonio Lucineudo de Oliveira Freire ◽  
Ivonete Alves Bakke ◽  
Cheila Deisy Ferreira ◽  
George Martins de França ◽  
...  
Keyword(s):  
Amino Acids ◽  
Water Deficit ◽  
Free Amino Acids ◽  
Free Amino ◽  
Soluble Sugars ◽  
Soluble Proteins ◽  
Organic Solutes ◽  
Total Soluble Sugars ◽  
Severe Water Deficit ◽  
Total Free Amino Acids

Drought stress negatively influences a variety of essential physiological process for plant growth and biomass production, and potassium contributes to the absorption of water and maintaining cell turgor, being crucial to understand the seedlings water stress responses. This research aimed to verify the effects to water deficiency and potassium on the growth and accumulation of organic solutes in Myracrodruon urundeuva, Libidibia ferrea, and Mimosa tenuiflora. The treatments were distributed in a 3 x 3 factorial scheme, with three water levels (100%pc - control, 50%pc - moderate water deficit, and 25%pc - severe water deficit) and three doses of potassium (0, 97.5 and 195 mg dm-3 K). The plants were sown in black plastic bags, containing 5 kg of soil. Were evaluated plant height, stem diameter, and leaf concentrations of total soluble sugars, total free amino acids, and proteins. The water deficit caused a reduction in the growth of M. urundeuva and M. tenuiflora plants, regardless of the added potassium. The plants of M. urundeuva and M.  tenuiflora were not demanding in potassium, while potassium fertilization with 97.5 mg dm-3 K favored L. ferrea plants, especially when kept under moderate water deficit. The moderate water deficit promoted accumulation of total free amino acids and soluble proteins in M. urundeuva, while in L. ferrea there was accumulation of total free amino acids under severe water deficit. Increase in the potassium promoted reduction in the concentrations of total soluble sugars and soluble proteins.

scholarly journals Silicon induces hormetic dose-response effects on growth and concentrations of chlorophylls, amino acids and sugars in pepper plants during the early developmental stage

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9224 ◽  
Author(s):  
Libia Iris Trejo-Téllez ◽  
Atonaltzin García-Jiménez ◽  
Hugo Fernando Escobar-Sepúlveda ◽  
Sara Monzerrat Ramírez-Olvera ◽  
Jericó Jabín Bello-Bello ◽  
...  
Keyword(s):  
Amino Acids ◽  
Developmental Stage ◽  
Free Amino Acids ◽  
Free Amino ◽  
Soluble Sugars ◽  
Early Developmental Stage ◽  
Total Soluble Sugars ◽  
Total Free Amino Acids ◽  
Early Developmental ◽  
Pepper Plants

Background Silicon (Si) is a beneficial element that has been proven to influence plant responses including growth, development and metabolism in a hormetic manner. Methods In the present study, we evaluated the effect of Si on the growth and concentrations of chlorophylls, total amino acids, and total sugars of pepper plants (Capsicum annuum L.) during the early developmental stage in a hydroponic system under conventional (unstressed) conditions. We tested four Si concentrations (applied as calcium silicate): 0, 60, 125 and 250 mg L−1, and growth variables were measured 7, 14, 21 and 28 days after treatment (dat), while biochemical variables were recorded at the end of the experiment, 28 dat. Results The application of 125 mg L−1 Si improved leaf area, fresh and dry biomass weight in leaves and stems, total soluble sugars, and concentrations of chlorophylls a and b in both leaves and stems. The amino acids concentration in leaves and roots, as well as the stem diameter were the highest in plants treated with 60 mg L−1 Si. Nevertheless, Si applications reduced root length, stem diameter and total free amino acids in leaves and stems, especially when applied at the highest concentration (i.e., 250 mg L−1 Si). Conclusion The application of Si has positive effects on pepper plants during the early developmental stage, including stimulation of growth, as well as increased concentrations of chlorophylls, total free amino acids and total soluble sugars. In general, most benefits from Si applications were observed in the range of 60–125 mg L−1 Si, while some negative effects were observed at the highest concentration applied (i.e., 250 mg L−1 Si). Therefore, pepper is a good candidate crop to benefit from Si application during the early developmental stage under unstressed conditions.

scholarly journals Effect of UV-B Radiation on Germination of Maize (Zea mays L.)

2017 ◽  
Vol 14 (3) ◽  
pp. 887-891
Author(s):  
Nagendram Erram ◽  
Anil Gaddameedi ◽  
Swapna Siddamalla ◽  
Tumu Venkat Reddy ◽  
Manjula Bhanoori
Keyword(s):  
Amino Acids ◽  
Soluble Protein ◽  
Free Amino Acids ◽  
Free Amino ◽  
Soluble Sugars ◽  
Germination Percentage ◽  
Total Soluble Protein ◽  
Total Soluble Sugars ◽  
Maize Seeds ◽  
Proline Concentration

ABSTRACT: In the present investigation, Maize seeds of hybrid NK 6240 and 900M Gold were exposed to UV-B (280-320 nm) for periods of 40 and 60 minutes and compared with the control without exposer to UV-B. The biochemical changes associated with UV-B induced resistance were investigated by determination of proline concentration, total soluble sugars, total soluble protein, malondialdehyde content and free amino acids from leaves and roots. Also comparison for germination percentage between control and treated seeds was carried along with biochemical traits. Analysis of variance explains both the hybrids were significantly different in germination percentage, total soluble sugars, proline and free amino acids. Whereas both treatments showed high significant variation for all the studied traits, but genotype × treatment interaction was non-significant for all the traits. 40 minutes UV-B treated seeds showed reduced total soluble sugars and increased malondialdehyde, proline and total soluble protein content. In 60 minutes UV-B treatment, decrease in free amino acids, proline, percentage of germination, and total soluble protein and increase in total soluble sugars was observed.

scholarly journals Seed Pretreatment and Foliar Application of Proline Regulate Morphological, Physio-Biochemical Processes and Activity of Antioxidant Enzymes in Plants of Two Cultivars of Quinoa (Chenopodium quinoa Willd.)

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 588
Author(s):  
Hira Yaqoob ◽  
Nudrat A. Akram ◽  
Samrah Iftikhar ◽  
Muhammad Ashraf ◽  
Noman Khalid ◽  
...  
Keyword(s):  
Amino Acids ◽  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Free Amino ◽  
Foliar Application ◽  
Soluble Sugars ◽  
Chenopodium Quinoa ◽  
Dry Weight ◽  
Total Soluble Sugars ◽  
Total Free Amino Acids

In the current study, the effects of exogenously applied proline (25 and 50 mM) and low-temperature treatment were examined on the physiochemical parameters in the plants of two cultivars (V1 and V2) of quinoa (Chenopodium quinoa Willd.). The seeds were also exposed to chilling stress at 4 °C before sowing. Plants raised from the seeds treated with low temperature showed reduced plant growth and contents of chlorophyll and carotenoids, but they had significantly increased contents of malondialdehyde, proline, ascorbic acid, total free amino acids, total soluble sugars, and total phenolics, as well as the activity of the peroxidase (POD) enzyme. Cold stress applied to seeds remained almost ineffective in terms of bringing about changes in plant root, hydrogen peroxide, glycine betaine and activities of superoxide dismutase (SOD), and catalase (CAT) enzymes. The exogenous application of proline significantly increased plant growth, the contents of chlorophyll, carotenoids, proline, ascorbic acid, total free amino acids, phenolics, and total soluble sugars, as well as the activities of SOD, POD, and CAT, but it decreased malondialdehyde content. Overall, foliar application of proline was better than the seed treatment in improving root dry weight, root length, chlorophyll a, carotenoids, glycine betaine, ascorbic acid and superoxide dismutase activity, whereas seed pre-treatment with proline was effective in improving shoot dry weight, shoot length, hydrogen peroxide, malondialdehyde, and peroxidase activity in both quinoa cultivars.

scholarly journals Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1147
Author(s):  
Bojana Živanović ◽  
Sonja Milić Komić ◽  
Tomislav Tosti ◽  
Marija Vidović ◽  
Ljiljana Prokić ◽  
...  
Keyword(s):  
Amino Acids ◽  
Abscisic Acid ◽  
Stomatal Conductance ◽  
Water Deficit ◽  
Free Amino Acids ◽  
Free Amino ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Parental Line ◽  
Suitable Model

Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.

Proteolysis and formation of volatile compounds in cheese manufactured with a bacteriocin-producing adjunct culture

2001 ◽  
Vol 68 (1) ◽  
pp. 117-129 ◽  
Author(s):  
ABDELGHANI OUMER ◽  
PILAR GAYA ◽  
ESTRELLA FERNÁNDEZ-GARCÍA ◽  
RAÚL MARIACA ◽  
SONIA GARDE ◽  
...  
Keyword(s):  
Amino Acids ◽  
Enterococcus Faecalis ◽  
Free Amino Acids ◽  
Free Amino ◽  
Leuconostoc Mesenteroides ◽  
Aminopeptidase Activity ◽  
Hydrophobic Peptides ◽  
Bacteriocin Producer ◽  
Total Free Amino Acids ◽  
Adjunct Culture

Hispánico cheese, a semi-hard Spanish variety, was manufactured from a mixture of pasteurized cows' and ewes' milks (4[ratio ]1) using a commercial mesophilic LD-type starter comprising Lactococcus lactis subsp. cremoris, Lc. lactis subsp. lactis, Lc. lactis subsp. lactis var diacetylactis and Leuconostoc mesenteroides subsp. cremoris. Varying amounts (0–1·0 g/kg) of an Enterococcus faecalis INIA 4 culture in milk were added as a bacteriocin-producing adjunct. Differences in pH between cheeses manufactured with and without the bacteriocin producer did not exceed 0·11 pH units. Starter lactococci lost viability more rapidly in cheeses made with the bacteriocin producer, which reached counts of up to 6×107 cfu/g during ripening. Aminopeptidase activity in 1-d-old cheese made from milk inoculated with 1·0 g bacteriocin-producing culture/kg was twice that in control cheese. Degrees of overall proteolysis and levels of total free amino acids in 45-d-old cheese made with 1·0 g bacteriocin-producing culture/kg were 1·80-fold and 2·17-fold those in control cheese of the same age. Inoculating milk with 1·0 g/kg bacteriocin-producing culture reduced the level of hydrophobic peptides in the resultant cheese, increased the concentrations of 3-methyl-1-butanal, diacetyl and acetoin, and resulted in the highest scores for flavour quality and flavour intensity throughout ripening.

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

Effects of ozone on 14CO2 fixation patterns in pine

1973 ◽  
Vol 51 (9) ◽  
pp. 1573-1578 ◽  
Author(s):  
Thomas G. Wilkinson ◽  
Robert L. Barnes
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Soluble Sugars ◽  
Pinus Strobus ◽  
Relative Activity ◽  
Ozone Treatment ◽  
White Pine ◽  
14Co2 Fixation ◽  
Sugar Phosphates

Seedlings of white pine (Pinus strobus L.) and detached shoots of white and loblolly (P. taeda L.) pines were exposed to various concentrations of ozone either before or during photosynthetic fixation of 14CO2. The major changes in distribution of 14C as a result of ozone treatment were (1) a reduction of relative activity in soluble sugars; (2) an increase in activity in sugar phosphates; and (3) an increase in activity in free amino acids, especially alanine. Significant differences in 14C fixation patterns were observed at ozone concentrations as low as 10 pphm (parts per hundred million), and during 14CO2 fixation times as short as 10 min. However, other combinations of treatment levels and times did not always result in statistically significant effects.

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