Overexpression of Rice Gene Lsi1 (Low Silicon Gene 1) Enhances The Plant-Microbe Interaction Resulting In Improved Chilling Tolerance

Chilling is an environmental phenomenon that hampers the plant growth. Related studies are mainly on based on the aerial plant parts. While, below ground to rhizosphere microbiome have been neglected under low temperature stress. The overexpression of Lsi1 in Dular significantly enhances its proline concentration compared with wild type Dular. This overexpression phenomenon maintains the osmotic balance of cells through influx of Ca2+, K+, H+ and efflux of Na+. The calcium deposition and the activity of plasma membrane H+-ATPase determined in root tip was consistent with the obtained ion flux results. In addition, the high-throughput sequencing results showed significant variation among identified 84 genera in different rhizocompartments (rhizosphere, rhizoplane and endosphere). The identified bacteria were associated with photosynthesis, energy metabolism, nitrogen fixation and defense, which were significantly increased in overexpressed plant. While, the number of pathogenic microorganisms associating were significantly reduced. Our findings suggests that the stability of ion balance in overexpressed plant roots affects the structure of microbial community in soil. To conclude, the over expression of Lsi1 gene enhanced cold tolerance of rice and its underlying mechanisms were comprehensively analyzed which can further impart its role in the stress tolerant rice plants.

Foliar-Applied Selenium Nanoparticles Alleviate Cadmium Stress Through Changes in Physio-Biochemical Status and Essential Oil Profile of Coriander (Coriandrumsativum L.) Leaves

Since large areas of agricultural soils around the world are contaminated by Cd, a cost-effective and practical method is needed for the safe production of edible plants. The effective role of many nanomaterials to improve plant yield and alleviate environmental pollutions is addressed; however, selenium nanoparticles (Se-NPs) potential has not been well-known yet. The aim of this work was to investigate foliar application of Se-NPs on yield, water content, proline concentration, phenolic content, lipid peroxidation, and essential oil (EO) attributes of coriander (Coriandrum sativum L.) under Cd stress. This study consisted of Cd contamination (0, 4, and 8 mg L-1) and foliar application of Se-NPs (0, 20, 40, and 60 mg L-1) in a hydroponic system. The results showed enhanced Cd concentration of root and shoot of coriander under Cd stress; however, Se-NPs alleviate the uptake of Cd. There were observed the decreased shoot and root weight, chlorophyll (Chl) content and relative water content (RWC) by progressing Cd toxicity, but Se-NPs improved these attributes. Moderate Cd stress and 40 or 60 mg L-1 Se-NPs increased phenolic and flavonoid contents as well as EO yield. Proline concentration and malondialdehid (MDA) increased by enhancing Cd stress, but Se-NPs decreased MDA. The GC/MS analysis showed that the main EO constitutes were n-decanal (18.80-29.70%), 2E-dodecanal (14.23-19.87%), 2E-decanal (12.60-19.40%), and n-nonane (7.23-12.87%), representing different amounts under Cd pollution and Se-NPs. To sum up, 40-60 mg L-1 Se-NPs are effective in alleviating Cd stress.

Effect of Foliar Application For Proline, Zinc Sulphate on Growth and Yield of Okra Plant (Abelmoschus Esculentus L.)

The field experiment was carried out at the Researches Station and Agricultural Experiments/College of Agriculture/Al-Muthanna University during the summer agricultural season 2020 on Okra plant. To study foliar spraying application of Proline from three levels (0, 40 and 80) mg.L−1 and gave P0, P1 and P2 respectively, Zinc sulphate (0, 20 and 40) mg.L−1 and given these Zn0, Zn1 and Zn2. Using the randomized complete block design (R.C.B.D.) and each treatment was treated in three replicates. Means were compared using L.S.D at 0.05 level of significance. The results of the experiment showed The treatment of P2 spraying with proline concentration (80 mg.L−1) was superior in the percentage of Nitrogen, phosphorus and potassium in leaves, Number of branches, Leaves area and Plant yield (g) (3.09%, 0.44%, and 2.88%, 9.04 branch plant−1, 0.723m2, 303.39 g.plant−1) respectively. The results also showed that Zn2 treatment was elevated (40 mg.L−1) on all measured indicators (3.29%, 0.37%, 2.83%, 8.76 branch plant−1, 0.763 m2, 334.35 g.plant−1). the outperformed was in the significant effect of interaction treatment proline and zinc sulphate P2Zn2 on all measured indicators.

Troglitazone-Induced PRODH/POX-Dependent Apoptosis Occurs in the Absence of Estradiol or ERβ in ER-Negative Breast Cancer Cells

The impact of estradiol on troglitazone (TGZ)-induced proline dehydrogenase/proline oxidase (PRODH/POX)-dependent apoptosis was studied in wild-type and PRODH/POX-silenced estrogen receptor (ER) dependent MCF-7 cells and ER-independent MDA-MB-231 cells. DNA and collagen biosynthesis were determined by radiometric method, prolidase activity evaluated by colorimetric method, ROS production was measured by fluorescence assay. Protein expression was determined by Western blot and proline concentration by LC/MS analysis. PRODH/POX degrades proline yielding reactive oxygen species (ROS). Estrogens stimulate collagen biosynthesis utilizing free proline and limiting its availability for PRODH/POX-dependent apoptosis. TGZ cytotoxicity was highly pronounced in wild-type MDA-MB-231 cells cultured in medium without estradiol or in the cells cultured in medium with estradiol but deprived of ERβ (by ICI-dependent degradation), while in PRODH/POX-silenced cells the process was not affected. The TGZ cytotoxicity was accompanied by increase in PRODH/POX expression, ROS production, expression of cleaved caspase-3, caspase-9 and PARP, inhibition of collagen biosynthesis, prolidase activity and decrease in intracellular proline concentration. The phenomena were not observed in PRODH/POX-silenced cells. The data suggest that TGZ-induced apoptosis in MDA-MB-231 cells cultured in medium without estradiol or deprived of ERβ is mediated by PRODH/POX and the process is facilitated by proline availability for PRODH/POX by TGZ-dependent inhibition of collagen biosynthesis. It suggests that combined TGZ and antiestrogen treatment could be considered in experimental therapy of estrogen receptor negative breast cancers.

Towards Accelerated Autolysis? Dynamics of Phenolics, Proteins, Amino Acids and Lipids in Response to Novel Treatments and during Ageing of Sparkling Wine

Premium sparkling wine produced by the traditional method (analogous to the French méthode champenoise) is characterised by the development of aged wine character as a result of a second fermentation in the bottle with lees contact and lengthy ageing. Treatments (microwave, ultrasound, or β-glucanase enzymes) were applied to disrupt the cell wall of Saccharomyces cerevisiae and added to the tirage liquor for the second fermentation of Chardonnay-Pinot Noir base wine cuvée and compared to a control, to assess effects on the release of phenolics, proteins, amino acids, and lipids at 6, 12 and 18 months post-tirage. General responses to wine ageing included a 60% increase in the total phenolic content of older sparkling wines relative to younger wines and an increase in protein concentration from 6 to 12 months bottle age. Microwave and β-glucanase enzyme treatments of yeast during tirage preparation were associated with a 10% increase in total free amino acid concentration and a 10% increase in proline concentration at 18 months bottle age, compared to control and ultrasound treatment. Furthermore, microwave treatment was associated with elevated asparagine content in wine at 18 months bottle age, relative to the control and the other wines. The β-glucanase enzyme and ultrasound treatments were associated with significant accumulation of total lipids, which were driven by 2-fold increases in the phospholipid and monoacylglycerol components in wine at 18 months bottle age and, furthermore, the microwave treatment was associated with elevated triacylglycerol at 18 months bottle age. This study demonstrates that the use of yeast treatments at the tirage stage of sparkling wine production presents an opportunity to manipulate wine composition.

Investigating the Drought and Salinity Effect on the Redox Components of Sulla coronaria (L.) Medik

For the Mediterranean region, climate models predict an acceleration of desertification processes, thus threatening agriculture. The present work aimed to investigate the effect of drought and salinity on Sulla coronaria (L.) Medik., a Mediterranean forage legume, for understanding plant defence systems activated by these stressors. In detail, we focused our attention on the variations on the plant redox status. Plants were subjected to suboptimal watering and irrigation with sodium chloride (NaCl) solutions. The same salt treatment was applied for in vitro tests on seedlings. Water content did not change after treatments. Salt negatively influenced seed germination and seedling development, but it did not affect photosynthesis parameters, contrary to what was observed in adult plants. Proline concentration increased in all samples, while abscisic acid level increased exclusively in seedlings. NaCl caused accumulation of superoxide anion in plants and seedlings and hydrogen peroxide only in seedlings; nevertheless, lipid peroxidation was not detected. Total phenolics, glutathione, expression level, and activity of antioxidant enzymes were assayed, revealing a complex antiradical molecular response, depending on the type of stress and development stage. Our results confirm Sulla as a drought- and salt-tolerant species and highlight its ability to counteract oxidative stress. This evidence suggests a key role for the redox components, as signal transduction messengers, in Sulla acclimation to desertification. Finally, plants and seedlings showed different acclimation capacity to salinity, revealing a greater genomic plasticity for seedlings.

Proline oxidase silencing inhibits p53-dependent apoptosis in MCF-7 breast cancer cells

Proline oxidase (POX) is mitochondrial proline-degrading enzyme of dual apoptosis/survival function. POX expression and proline availability are considered an underlying mechanism for differential POX functions. The mechanism for POX-dependent regulation of cell death/survival was studied in wild-type (MCF-7WT) and shRNA POX-silenced breast cancer cells (MCF-7iPOX). Proline concentration and proteomic analyses were determined by LC/MS/QTOF and LC/MS/ORBITRA, respectively. Inhibition of collagen biosynthesis (proline utilizing process) by 2-methoxyestradiol (2ME) contributed to induction of apoptosis in MCF-7WT cells, as detected by increase in the expression of active caspase-3, -9 and p53. The process was not shown in MCF-7iPOX. In MCF-7iPOX cells prolidase activity and expression as well as proline concentration were drastically increased, compared to MCF-7WT cells. Down-regulation of p53 in MCF-7iPOX cells was corroborated by proteomic analysis showing decrease in the expression of p53-related proteins. The mechanism for down-regulation of p53 expression in MCF-7iPOX cells was found at the level of p53–PEPD complex formation that was counteracted by hydrogen peroxide treatment. In this study, we found that silencing POX modulate pro-survival phenotype of MCF-7 cells and suggest that the mechanism of this process undergoes through down-regulation of p53-dependent signaling.

L-Proline Activates Mammalian Target of Rapamycin Complex 1 and Modulates Redox Environment in Porcine Trophectoderm Cells

L-proline (proline) is a key regulator of embryogenesis, placental development, and fetal growth. However, the underlying mechanisms that support the beneficial effects of proline are largely unknown. This study used porcine trophectoderm cell line 2 (pTr2) to investigate the underlying mechanisms of proline in cell proliferation and redox homeostasis. Cells were cultured in the presence of 0, 0.25, 0.50, or 1.0 mmol/L proline for an indicated time. The results showed that 0.5 and 1.0 mmol/L proline enhanced cell viability. These effects of proline (0.5 mmol/L) were accompanied by the enhanced protein abundance of p-mTORC1, p-p70S6K, p-S6, and p-4E-BP1. Additionally, proline dose-dependently enhanced the mRNA expression of proline transporters [solute carrier family (SLC) 6A20, SLC36A1, SLC36A2, SLC38A1, and SLC38A2], elevated proline concentration, and protein abundance of proline dehydrogenase (PRODH). Furthermore, proline addition (0.25 or 0.5 mmol/L) resulted in lower abundance of p-AMPKα when compared with a control. Of note, proline resulted in lower reactive oxygen species (ROS) level, upregulated mRNA expression of the catalytic subunit of glutamate–cysteine ligase (GCLC) and glutathione synthetase (GSS), as well as enhanced total (T)-GSH and GSH concentration when compared with a control. These data indicated that proline activates themTORC1 signaling and modulates the intracellular redox environment via enhancing proline transport.

Effect of Diluted Red Sea Water on Growth Behavior and Chemical Component of Moringa Plants

Background: Moringa tree is a drought resistance tree, fast-growing, Moringa leaves, flowers and pods are a food source for human and animals. It is a rich source of calcium, iron and a good source of vitamins, B, A, C, amino acids. Shortage of water resources for irrigation especially in reclaimed soil considered is one of the great problems to cultivate traditional crops. Thus, nowadays the use of the non-traditional source of water, such as saline water is very important. Results: The obtained data recorded that the lowest level of salinity (4000 ppm) decreased plant height, fresh and dry weight of leaves, the stem fresh and dry weight, stem diameter recorded decreased. Increasing salinity levels up to 16000 ppm high reduction was registered in the previously mentioned parameters except for leaves number. The interaction between salinity and proline concentration exhibited in most cases reduction in total chlorophyll. Carbohydrate content, protein, nitrogen%, calcium%, recorded reduction in most cases under saline condition and their interaction with proline. While, Sodium %, Cl- percentage and proline content recorded increments in most cases under the previously mentioned circumstances. Conclusion: The results were nearly ascending order with increasing of salinity level. Increasing salinity to 16000 ppm decreased significantly the values of vegetative growth and almost all cases of chemicals constituents were reduced.

Proline Concentration and Its Metabolism Are Regulated in a Leaf Age Dependent Manner But Not by Abscisic Acid in Pea Plants Exposed to Cadmium Stress

The accumulation of proline is one of the defense mechanisms of plants against the harmful effects of adverse environmental conditions; however, when pea plants were treated for 12 h with CdCl2, the proline concentration decreased in the youngest A (not expanded) and B1 (expanded) leaves, and did not change significantly in the B2 (mature, expanded) or C (the oldest) leaves. After 24 h of cadmium (Cd) stress, the proline concentration remained low in A and B1 leaves, while in B2 and C leaves, it increased, and after 48 h, an increase in the proline concentration in the leaves at each stage of development was observed. The role of proline in the different phases of plant response to the Cd treatment is discussed. Changes in proline accumulation corresponded closely with changes in the transcript levels of PsP5CS2, a gene encoding D1-pyrroline-5-carboxylate synthetase involved in proline synthesis, and PsPDH1, a gene encoding proline dehydrogenase engaged in proline degradation. CdCl2 application induced the expression of PsProT1 and PsProT2, genes encoding proline transporters, especially during the first 12 h of treatment in A and B1 leaves. When the time courses of abscisic acid (ABA) and proline accumulation were compared, it was concluded that an increase in the proline concentration in the leaves of Cd-treated pea plants was more related to a decrease in chlorophyll concentration (leaves B2 and C) and an increase in the malondialdehyde level (A and B1 leaves) than with an increase in ABA concentration alone. Exogenous application of ABA (0.5, 5, 50 µM) significantly increased the proline concentration in the A leaves of pea plants only, and was accompanied by an elevated and repressed expression of PsP5CS2 and PsPDH1 in these leaves, respectively. The presented results suggest that under Cd stress, the accumulation of proline in leaves of pea plants may take place independently of the ABA signaling.