Application of auxin-cytokinin substitute in vitro culture Solanacea crops

Aim. The purpose of our research was to establish the possibility of initiation of potato and tomato culture plants using industrial growth regulators, which are legal for use in Ukraine, which include substances with pronounced auxin-cytokinin activity. Methods. In this work, we used varieties of tomato: Khoriv, Borivsky and Bozhedar, Povin potatos. Work in culture in vitro was performed according to conventional methods. Results. A phytohormone substitute was created in a known nutrient agar medium according to Murasige-Skuga. For the in vitro cultivation of tomato and potato plants, phytohormones were replaced by solutions of Ecostim and Ecostim 1, which exhibited auxin-cytokinin activity. The cost of these substitutes is much lower than that of commercial phytohormones. Conclusions. It is shown that optimal for growth in the MS medium in the callusogenesis of Solanacea cultures in vitro. That variant with the use of cytokinin substitutes Ecostim and Ecostim 1 with the rate of using of 35.0 and 40.0 mg/L. Keywords: modification of MS medium, potatoes, tomatoes, cell culture in vitro.

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Development of a method for obtaining healthy mini-tubers of potatoes of the Yubilyar variety using a humic preparation from peat

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/928/1/012009 ◽

2021 ◽

Vol 928 (1) ◽

pp. 012009

Author(s):

M S Romanova ◽

E V Khaksar ◽

N I Leonova ◽

O O Novikov ◽

E I Kosinova ◽

...

Keyword(s):

Survival Rate ◽

Nutrient Medium ◽

In Vitro Cultivation ◽

Potato Plants ◽

Mineral Components ◽

Ground Conditions ◽

The Cost ◽

Biological Fertilizer ◽

Optimal Medium

Abstract The influence of various nutrient medium compositions on the growth of potato plants during in vitro cultivation and the possibility of using biological fertilizer from peat in different concentrations to improve the adaptation of plants to growing in ground for the development of a method for obtaining healthy mini-tubers of potatoes of the Yubilyar variety were studied. The influence of various nutrient medium compositions on the height, biomass, intensity of rhizogenesis, the number of internodes of improved potato micro-plants, as well as humic fertilizer from Humostim peat on the survival rate and height of potato plants when adapting it to ground conditions is analyzed. Taking into account the data obtained, as well as the cost of the studied variants of the medium, the optimal medium for growing potato micro-plants in laboratory conditions in vitro is a nutrient medium with a content of mineral components 1/3 from the norm. The use of humic preparation from peat Humostim in a concentration of 0.001% caused an acceleration of plant growth and an increase in their height, and this concentration is recommended for use.

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In vitro Shoot Cultures of Tupistra albiflora K. Larsen

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2020.4182 ◽

2018 ◽

Vol 17 (5) ◽

pp. 405-411

Author(s):

Jiraporn PALEE

Keyword(s):

Agar Medium ◽

Multiple Shoots ◽

Shoot Formation ◽

Multiple Shoot ◽

Naphthalene Acetic Acid ◽

Root Induction ◽

Shoot Cultures ◽

Ms Medium ◽

In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

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Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae)

Rodriguésia ◽

10.1590/2175-7860202172018 ◽

2021 ◽

Vol 72 ◽

Author(s):

Rosiane Cipriano ◽

João Paulo Rodrigues Martins ◽

Luiz Carlos de Almeida Rodrigues ◽

Antelmo Ralph Falqueto ◽

Andreia Barcelos Passos Lima Gontijo

Keyword(s):

Photosystem Ii ◽

Saline Solution ◽

Growth Traits ◽

Photosynthetic Apparatus ◽

Solution Culture ◽

In Vitro Cultivation ◽

Ms Medium ◽

Number Of Leaves ◽

Positive Effect

Abstract In vitro cultivation is a technique with wide application for micropropagation. However, each species has specific mineral needs for this type of cultivation. The objective was to assess the impacts of the saline solution culture medium on the performance of the photosynthetic apparatus and growth of Bromelia antiacantha during in vitro cultivation, and thus to elucidate the mitigation of the nutritional imbalance that can interfere in the electron transport in the plants. Plants were cultivated in a salt concentration gradient of MS medium (0%, 25%, 50%, 75% or 100%). The growth traits and fluorescence a chlorophyll were analyzed. Intermediate concentrations of MS medium resulted in plants with a larger number of leaves and longer root length. The OJIP curves and results of the JIP test showed that the plants grown without MS salts presented less efficient photosystem II (PSII), as indicated by the performance index [Pi(total)]. In contrast, the intermediate concentrations (MS 25% and 50%) had a positive effect on the performance of the photosynthetic apparatus. The MS 25% medium can be used for in vitro cultivation of B. antiacantha, enabling the development of plants with suitable physiological qualities for planting in the field.

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In Vitro Seed Germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.48.23 ◽

2015 ◽

Vol 48 ◽

pp. 23-31 ◽

Cited By ~ 3

Author(s):

S. Kaveri ◽

Rao Srinath

Keyword(s):

Seed Germination ◽

Embryo Culture ◽

Filter Paper ◽

Agar Medium ◽

Ms Medium ◽

Mature Embryos ◽

Nothapodytes Foetida ◽

Half Strength ◽

Paper Bridge

In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA3, H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA3 and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

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Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

Revista Colombiana de Ciencias Hortícolas ◽

10.17584/rcch.2019v13i2.8034 ◽

2019 ◽

Vol 13 (2) ◽

pp. 269-278

Author(s):

Adriano Bortolotti Silva ◽

Ligiane Aparecida Florentino ◽

Dalvana De Sousa Pereira ◽

Paulo Roberto Correa Landgraf ◽

Ana Carolina Rodrigues Alves ◽

...

Keyword(s):

Shoot Growth ◽

Ex Vivo ◽

Control Treatment ◽

In Vitro Cultivation ◽

Ms Medium ◽

Ex Vitro ◽

Diazotrophic Bacteria ◽

Bacterial Strains ◽

Indole 3 Acetic Acid

Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity.

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In vitro Cultivation of Potato Plants

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2016.512.094 ◽

2016 ◽

Vol 5 (12) ◽

pp. 858-868 ◽

Cited By ~ 1

Author(s):

Ibrahim A. Ibrahim ◽

H.A. Emara ◽

A.A. Nower ◽

A.Y. Abodiab

Keyword(s):

In Vitro Cultivation ◽

Potato Plants

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In vitro culture of jambu with different growth regulators

Horticultura Brasileira ◽

10.1590/s0102-053620200204 ◽

2020 ◽

Vol 38 (2) ◽

pp. 134-138

Author(s):

Susan P Almeida ◽

Joanne MM Souza ◽

Andredy MT Amorim ◽

Sérgio AL de Gusmão ◽

Rodrigo ORM Souza ◽

...

Keyword(s):

Growth Regulators ◽

Callus Induction ◽

Nodal Segments ◽

Root Production ◽

In Vitro Cultivation ◽

Average Height ◽

Ms Medium ◽

Fresh Matter ◽

Friable Callus

ABSTRACT The aim of this study was to establish the best concentrations of growth regulators for in vitro cultivation of jambu for a subsequent elaboration of an efficient micropropagation protocol. After sterilized, the seeds were inoculated on different media (MS, ½MS and water-agar) for in vitro germination. Nodal segments of in vitro germinated jambu seedlings were used as explants in the micropropagation with different concentrations of 6-benzylaminopurine (BAP) (0.0; 0.125; 0.25; 0.50 and 0.75 mg L-1) and callus induction with 2.4- dichlorophenoxyacetic acid (2.4-D) (0.0; 0.25; 0.50; 0.75 and 1.0 mg L-1) on Murashige & Skoog’s (MS) medium. The highest germination rates were obtained on MS medium with better seedling development and greater height (3.7cm). In micropropagation, the best treatment was obtained on 0.125 BAP (T2), with an average of 2.2 sprouts/explant, average height of 2.4 cm and vigorous sprouts. In callus induction, all treatments with 2.4-D had developed friable calluses in 30 days and using doses of 0.25 and 0.50 mg L-1 provided greater fresh matter. The induction of friable callus and the root production occur without supplementation of exogenous growth regulator.

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Propagation of Lily Arumsari using Generic Media through In Vitro Culture

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.11.2.140-148 ◽

2020 ◽

Vol 11 (2) ◽

pp. 140-148

Author(s):

Ridho Kurniati ◽

Fauziah Khairatunnisa ◽

Reni Indrayanti

Keyword(s):

Plant Growth Regulator ◽

Low Cost ◽

Ms Medium ◽

Single Factor ◽

Mass Propagation ◽

Murashige And Skoog Medium ◽

Number Of Leaves ◽

Leaves And Roots ◽

The Cost

Lily was usually propagated using MS (Murashige and Skoog) medium supplemented with plant growth regulator and hormone. The important concerns in mass propagation and seed production of lily for industries are cheap, easy and low cost medium. This study’s objective was to find out of generic media to substitute MS medium and to decrease the cost of mass propagation of lily in vitro. Three substitutions medium were Vitagrow, Growmore, Gibril, and G-60. Bulbs of lily Arumsari varieties were used as materials. Complete Random Design with a single factor was used in the experimental design. The treatments were type of generic in vitro medium (Vitagrow, Growmore, Gibril, and G-60), consisting of three replication and 20 units per repetition and five bulbs per unit of repetition. The parameter observed was the total number of leaves and roots, length of leaves and roots, and bulb growth percentage. Growmore medium showed a better result than others in total number of leaves (50,67), length of leaves (1,6 cm), length of roots (0,312 cm) and percentage of bulb growth (100%). The highest total number of roots was achieved in G-60 medium.

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In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb

Molecules ◽

10.3390/molecules26195888 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5888

Author(s):

Desislava I. Mantovska ◽

Miroslava K. Zhiponova ◽

Milen I. Georgiev ◽

Tsvetinka Grozdanova ◽

Dessislava Gerginova ◽

...

Keyword(s):

Ex Situ ◽

Nodal Segments ◽

Cultivated Plants ◽

In Vitro Cultivation ◽

Ms Medium ◽

Ex Vitro ◽

Biotechnological Approach ◽

Iridoid Glucosides ◽

Development In Vitro

Micropropagation of rare Veronica caucasica M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.0 mg L–1 6-benzylaminopurine (BA) and then transferring the regenerated plants on hormone free basal MS medium for root development. In vitro multiplicated plants were successively acclimated in a growth chamber and a greenhouse with 92% survival. The number of plastid pigments and the total phenolics content in in vitro cultivated and ex vitro adapted plants were unchanged, and no accumulation of reactive oxygen species (ROS) was detected by staining with 3-3′-diaminobenzidine (DAB) and 2′,7′-dichlorofluorescein diacetate (DCF-DA). Nuclear Magnetic Resonance (NMR) fingerprinting allowed for the identification of the major alterations in metabolome of V. caucasica plants during the process of ex situ conservation. Iridoid glucosides such as verproside, aucubin and catalpol were characteristic for in vitro cultivated plants, while in ex vitro acclimated plants phenolic acid–protocatechuic acid and caffeic acid appeared dominant. The successful initiation of in vitro and ex vitro cultures is an alternative biotechnological approach for the preservation of V. caucasica and would allow for further studies of the biosynthetic potential of the species and the selection of lines with a high content of pharmaceutically valuable molecules and nutraceuticals.

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Nitrogen assimilation in the bromeliad Ananas comosus var. ananassoides (Baker) Coppens & F.Leal grown in vitro with different sources of inorganic nitrogen

Hoehnea ◽

10.1590/2236-8906-96/2019 ◽

2020 ◽

Vol 47 ◽

Author(s):

Priscila Primo Andrade Silva ◽

Ivomar Aparecido Medina ◽

Jorge Luiz Marx Young ◽

Vívian Tamaki

Keyword(s):

Nitrogen Assimilation ◽

Inorganic Nitrogen ◽

Ananas Comosus ◽

In Vitro Cultivation ◽

Ms Medium ◽

Ex Vitro ◽

N Assimilation ◽

Modified Ms Medium ◽

Different Sources

ABSTRACT Ananas comosus var. ananassoides (Baker) Coppens & F.Leal is a native ornamental bromeliad of the endangered biome Cerrado. Therefore, approaches aimed at the preservation of this species, such as in vitro cultivation and micropropagation are needed. Nitrogen (N) is absorbed by plants, mainly as NO3- and/or NH4+, and assimilated into amino acids. The aim of this work was to evaluate the N assimilation in this bromeliad. Plants were grown in vitro for seven months in modified MS medium with 15, 30, 60, and 90 mM of N as NO3-, NH4+ or NH4NO3, and then transferred to ex vitro conditions for acclimatization. Plants grown with NH4+ had high mortality. During acclimatization plants cultivated with 30, 60, and 90 mM of N as NH4NO3 showed higher biomass. With regard to N assimilation, GS and NR showed the highest activity in plants cultivated with NH4NO3, whereas plants cultivated with NH4+ had the highest GDH activity. Consequently, in vitro and ex vitro cultivation of this species with 60 mM N as NH4NO3 is recommended.

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