scholarly journals Mir-484 contributes to diminished ovarian reserve by regulating granulosa cell function via YAP1-mediated mitochondrial function and apoptosis

2022 ◽  
Vol 18 (3) ◽  
pp. 1008-1021
Author(s):  
Huiying Li ◽  
Xiaofei Wang ◽  
Hongbei Mu ◽  
Qiaojuan Mei ◽  
Yu Liu ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Mitochondrial Function ◽  
Ovarian Reserve ◽  
Cell Function ◽  
Diminished Ovarian Reserve

scholarly journals Differential Granulosa Cell Gene Expression in Young Women with Diminished Ovarian Reserve

2011 ◽  
Vol 18 (9) ◽  
pp. 892-899 ◽  
Author(s):  
Keri Greenseid ◽  
Sangita Jindal ◽  
Joshua Hurwitz ◽  
Nanette Santoro ◽  
Lubna Pal
Keyword(s):  
Gene Expression ◽  
Granulosa Cell ◽  
Young Women ◽  
Ovarian Reserve ◽  
Diminished Ovarian Reserve ◽  
Cell Gene Expression ◽  
Cell Gene

scholarly journals miR-106a Increases Granulosa Cell Viability and Is Downregulated in Women With Diminished Ovarian Reserve

2018 ◽  
Vol 103 (6) ◽  
pp. 2157-2166 ◽  
Author(s):  
Liming Hong ◽  
Sha Peng ◽  
Ying Li ◽  
Ying Fang ◽  
Qin Wang ◽  
...  
Keyword(s):  
Cell Viability ◽  
Granulosa Cell ◽  
P38 Mapk ◽  
Granulosa Cells ◽  
Ovarian Reserve ◽  
Mirna Expression ◽  
Diminished Ovarian Reserve ◽  
Differentially Expressed ◽  
Mitogen Activated Protein Kinase

Abstract Context Women with diminished ovarian reserve (DOR) have reduced fertility, cardiovascular events, and osteoporosis. Although differential microRNA (miRNA) expression has been described in several ovarian disorders, little is known about the role of miRNAs in the pathogenesis of DOR. Objective Identify differentially expressed miRNAs in DOR and explore the role of miR-106a in human granulosa cell proliferation. Design miRNA microarray (n = 3) and quantitative reverse transcription polymerase chain reaction (n = 30) were used to examine miRNA expression in serum and granulosa cells from normal-cycling and women with DOR. Primary human granulosa cells were treated alone or in combination with miR-106a mimic, miR-106a inhibitor, apoptosis signal-regulating kinase 1 (ASK1) small interfering RNA (siRNA), or p38 mitogen-activated protein kinase (MAPK) inhibitor (SB203580) before assessment of cell viability and apoptosis. Western blot was used to measure ASK1 protein and phosphorylation/activation of p38 MAPK. Binding of miR-106a to ASK1 mRNA was examined by 3′ untranslated region (3′UTR) luciferase analysis. Results Fifteen miRNAs were differentially expressed (n = 30), and miR-106a was downregulated in serum and granulosa cells of women with DOR. miR-106a mimic increased cell viability and attenuated apoptosis, whereas the converse occurred following treatment with miR-106a inhibitor. miR-106a suppressed ASK1 expression by directly targeting its 3′UTR. miR-106a inhibitor increased p38 MAPK phosphorylation/activation, and this effect was abolished by treatment with ASK1 siRNA. Whereas knockdown of ASK1 abolished the effects of miR-106a inhibitor on cell viability/apoptosis, pretreatment with SB203580 did not significantly alter the effects of miR-106a inhibitor. Conclusions Downregulation of miR-106a may contribute to the pathogenesis of DOR by reducing granulosa cell viability and promoting apoptosis via enhanced ASK1 signaling.

P-202 Diminished ovarian reserve in women with advanced endometriosis

1997 ◽  
Vol 68 ◽  
pp. S188-S189
Author(s):  
D.L Hock ◽  
L.S Dagostino ◽  
E Kemmann
Keyword(s):  
Ovarian Reserve ◽  
Diminished Ovarian Reserve

Implications of diminished ovarian reserve (DOR) extend well beyond reproductive concerns

2008 ◽  
Vol 15 (6) ◽  
pp. 1086-1094 ◽  
Author(s):  
Lubna Pal ◽  
Kris Bevilacqua ◽  
Gohar Zeitlian ◽  
Jun Shu ◽  
Nanette Santoro
Keyword(s):  
Ovarian Reserve ◽  
Diminished Ovarian Reserve ◽  
Reproductive Concerns

scholarly journals The role of IGFs in the regulation of ovarian follicular growth in the brushtail possum (Trichosurus vulpecula)

Reproduction ◽  
2010 ◽  
Vol 140 (2) ◽  
pp. 295-303 ◽  
Author(s):  
Jennifer L Juengel ◽  
Lisa J Haydon ◽  
Brigitta Mester ◽  
Brian P Thomson ◽  
Michael Beaumont ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Function ◽  
Antral Follicle ◽  
Brushtail Possum ◽  
Follicular Growth ◽  
Theca Cells ◽  
Ovarian Follicular Growth

IGFs are known to be key regulators of ovarian follicular growth in eutherian mammals, but little is known regarding their role in marsupials. To better understand the potential role of IGFs in the regulation of follicular growth in marsupials, expression of mRNAs encoding IGF1, IGF2, IGF1R, IGF-binding protein 2 (IGFBP2), IGFBP4 and IGFBP5 was localized by in situ hybridization in developing ovarian follicles of the brushtail possum. In addition, the effects of IGF1 and IGF2 on granulosa cell function were tested in vitro. Both granulosa and theca cells synthesize IGF mRNAs, with the theca expressing IGF1 mRNA and granulosa cell expressing IGF2 mRNA. Oocytes and granulosa cells express IGF1R. Granulosa and theca cells expressed IGFBP mRNAs, although the pattern of expression differed between the BPs. IGFBP5 mRNA was differentially expressed as the follicles developed with granulosa cells of antral follicles no longer expressing IGFBP5 mRNA, suggesting an increased IGF bioavailability in the antral follicle. The IGFBP protease, PAPPA mRNA, was also expressed in granulosa cells of growing follicles. Both IGF1 and IGF2 stimulated thymidine incorporation but had no effect on progesterone production. Thus, IGF may be an important regulator of ovarian follicular development in marsupials as has been shown in eutherian mammals.

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