Comparative analysis of mRNA transcripts of HT-29 cell line expressed in identical quantities for pathogenic E. coli strains UM146 and UM147 with control Escherichia coli Nissle 1917

ABSTRACT The ability of Escherichia coli and Salmonella isolates to attach to Caco-2 and HT-29 cell monolayers was measured. All isolates displayed a greater ability to attach to Caco-2 cells than HT-29 cells, and overall E. coli isolates attached better to both cell lines than Salmonella isolates. Bacteria that were considered to be pathogenic displayed no greater ability to attach to cell lines than those that were not considered to be pathogenic. Additionally, no correlation was found between cell line attachment and previously determined hydrophobicity results.

Download Full-text

P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy

Epidemiology and Infection ◽

10.1017/s0950268898001137 ◽

1998 ◽

Vol 121 (3) ◽

pp. 599-608 ◽

Cited By ~ 15

Author(s):

I. ADLERBERTH ◽

C. SVANBORG ◽

B. CARLSSON ◽

L. MELLANDER ◽

L.-Å. HANSON ◽

...

Keyword(s):

Escherichia Coli ◽

Cell Line ◽

Intestinal Epithelial Cells ◽

Antigen Expression ◽

Intestinal Epithelial ◽

E Coli ◽

O Antigen ◽

Ht 29 ◽

Intestinal Persistence

Resident and transient Escherichia coli strains were identified in the rectal flora of 22 Pakistani infants followed from birth to 6 months of age. All strains were tested for O-antigen expression, adhesin specificity (P fimbriae, other mannose-resistant adhesins or type 1 fimbriae) and adherence to the colonic cell line HT-29. Resident strains displayed higher mannose- resistant adherence to HT-29 cells, and expressed P fimbriae (P=0·0036) as well as other mannose-resistant adhesins (P=0·012) more often than transient strains. In strains acquired during the first month of life, P fimbriae were 12 times more frequent in resident than in transient strains (P=0·0006). The O-antigen distribution did not differ between resident and transient strains, and none of the resident P-fimbriated strains belonged to previously recognized uropathogenic clones. The results suggest that adhesins mediating adherence to intestinal epithelial cells, especially P fimbriae, enhance the persistence of E. coli in the large intestine of infants.

Download Full-text

Induction of Human β-Defensin 2 by the Probiotic Escherichia coli Nissle 1917 Is Mediated through Flagellin

Infection and Immunity ◽

10.1128/iai.01563-06 ◽

2007 ◽

Vol 75 (5) ◽

pp. 2399-2407 ◽

Cited By ~ 189

Author(s):

Miriam Schlee ◽

Jan Wehkamp ◽

Artur Altenhoefer ◽

Tobias A. Oelschlaeger ◽

Eduard F. Stange ◽

...

Keyword(s):

Escherichia Coli ◽

Culture Supernatant ◽

Deletion Mutants ◽

Strain Atcc ◽

Bacterial Strains ◽

Flagellin Gene ◽

E Coli ◽

Escherichia Coli Nissle 1917 ◽

Stimulatory Factor ◽

Escherichia Coli Nissle

ABSTRACT Human β-defensin 2 (hBD-2) is an inducible antimicrobial peptide synthesized by the epithelium to counteract bacterial adherence and invasion. Proinflammatory cytokines, as well as certain bacterial strains, have been identified as potent endogenous inducers. Recently, we have found that hBD-2 induction by probiotic Escherichia coli Nissle 1917 was mediated through NF-κB- and AP-1-dependent pathways. The aim of the present study was to identify the responsible bacterial factor. E. coli Nissle 1917 culture supernatant was found to be more potent than the pellet, indicating a soluble or shed factor. Chemical analysis demonstrated the factor to be heat resistant and proteinase digestible. Several E. coli Nissle 1917 deletion mutants were constructed and tested for their ability to induce hBD-2 expression in Caco-2 cells. Deletion mutants for flagellin specifically exhibited an impaired immunostimulatory capacity. Reinsertion of the flagellin gene restored the induction capacity to normal levels. Isolated flagellin from E. coli Nissle 1917 and from Salmonella enterica serovar Enteritidis induced hBD-2 mRNA significantly in contrast to the flagellin of the apathogenic E. coli strain ATCC 25922. H1 flagellin antiserum abrogated hBD-2 expression induced by flagellin as well as E. coli Nissle 1917 supernatant, confirming that flagellin is the major stimulatory factor of E. coli Nissle 1917.

Download Full-text

The probiotic Escherichia coli Nissle 1917 inhibits propagating colonic contractions in the rat isolated large intestine

Food & Function ◽

10.1039/c4fo00831f ◽

2015 ◽

Vol 6 (1) ◽

pp. 256-263 ◽

Cited By ~ 8

Author(s):

J. E. Dalziel ◽

V. Mohan ◽

J. Peters ◽

R. C. Anderson ◽

P. K. Gopal ◽

...

Keyword(s):

Escherichia Coli ◽

Large Intestine ◽

Ex Vivo ◽

Colonic Motility ◽

E Coli ◽

Escherichia Coli Nissle 1917 ◽

Escherichia Coli Nissle

E. coli Nissle 1917 probiotic extract inhibits colonic motility in the rat large intestine ex-vivo, consistent with its anti-diarrheal effect in humans.

Download Full-text