scholarly journals Long range gene-regulatory sequences identified by transgenic expression of bacterially-engineered enhancer-trap BACs in zebrafish

2014 ◽  
Vol 2 (1) ◽  
pp. 2 ◽  
Author(s):  
Hope M Wolf ◽  
Kevin O Nyabera ◽  
Katya K De La Torre ◽  
Mugtaba A Eltayeb ◽  
Oladoyin Iranloye ◽  
...  
Keyword(s):  
Long Range ◽  
Enhancer Trap ◽  
Regulatory Sequences ◽  
Transgenic Expression ◽  
Gene Regulatory

Long-range cooperativity between gene regulatory sequences in a prokaryote

Nature ◽  
1987 ◽  
Vol 325 (6107) ◽  
pp. 823-826 ◽  
Author(s):  
Gert Dandanell ◽  
Poul Valentin-Hansen ◽  
Jens Erik Løve Larsen ◽  
Karin Hammer
Keyword(s):  
Long Range ◽  
Regulatory Sequences ◽  
Gene Regulatory

Target sequences for hunchback in a control region conferring Ultrabithorax expression boundaries

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1171-1179 ◽  
Author(s):  
C.C. Zhang ◽  
J. Muller ◽  
M. Hoch ◽  
H. Jackle ◽  
M. Bienz
Keyword(s):  
Long Range ◽  
Control Region ◽  
Binding Sites ◽  
Regulatory Sequences ◽  
Stripe Pattern ◽  
Anteroposterior Axis ◽  
Target Sequences ◽  
Beta Galactosidase ◽  
Control Regions

Boundaries of Ultrabithorax expression are mediated by long-range repression acting through the PBX or ABX control region. We show here that either of these control regions confers an early band of beta-galactosidase expression which is restricted along the anteroposterior axis of the blastoderm embryo. This band is succeeded by a stripe pattern with very similar anteroposterior limits. Dissection of the PBX control region demonstrates that the two patterns are conferred by distinct cis-regulatory sequences contained within separate PBX subfragments. We find several binding sites for hunchback protein within both PBX subfragments. Zygotic hunchback function is required to prevent ectopic PBX expression. Moreover, the PBX pattern is completely suppressed in embryos containing uniformly distributed maternal hunchback protein. Our results strongly suggest that hunchback protein directly binds to the PBX control region and acts as a repressor to specify the boundary positions of the PBX pattern.

scholarly journals Functional effects of variation in transcription factor binding highlight long-range gene regulation by epromoters

2019 ◽  
Author(s):  
Joanna Mitchelmore ◽  
Nastasiya Grinberg ◽  
Chris Wallace ◽  
Mikhail Spivakov
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Statistical Power ◽  
Target Genes ◽  
Allelic Variation ◽  
Regulatory Function ◽  
Regulatory Sequences ◽  
Distal Enhancer ◽  
Association Testing ◽  
Gene Regulatory

AbstractIdentifying DNA cis-regulatory modules (CRMs) that control the expression of specific genes is crucial for deciphering the logic of transcriptional control. Natural genetic variation can point to the possible gene regulatory function of specific sequences through their allelic associations with gene expression. However, comprehensive identification of causal regulatory sequences in brute-force association testing without incorporating prior knowledge is challenging due to limited statistical power and effects of linkage disequilibrium. Sequence variants affecting transcription factor (TF) binding at CRMs have a strong potential to influence gene regulatory function, which provides a motivation for prioritising such variants in association testing. Here, we generate an atlas of CRMs showing predicted allelic variation in TF binding affinity in human lymphoblastoid cell lines (LCLs) and test their association with the expression of their putative target genes inferred from Promoter Capture Hi-C and immediate linear proximity. We reveal over 1300 CRM TF-binding variants associated with target gene expression, the majority of them undetected with standard association testing. A large proportion of CRMs showing associations with the expression of genes they contact in 3D localise to the promoter regions of other genes, supporting the notion of ‘epromoters’: dual-action CRMs with promoter and distal enhancer activity.

Co-expression of insulin and somatostatin genes in pancreatic endocrine cells selected for their high level of insulin gene transcription

1992 ◽  
Vol 101 (4) ◽  
pp. 795-799
Author(s):  
C. Saulnier-Michel ◽  
M. Fromont-Racine ◽  
R. Pictet
Keyword(s):  
Endocrine Cells ◽  
Selective Pressure ◽  
Cell Types ◽  
Insulin Gene ◽  
Regulatory Sequences ◽  
Endogenous Insulin ◽  
Pancreatic Endocrine Cells ◽  
Gene Regulatory ◽  
High Level ◽  
Two Populations

RW cells are pancreatic endocrine RIN cells that have been stably transfected with a chimeric gene that places the expression of the dominant selection gpt gene under the control of the insulin gene regulatory sequences. These RW cells were examined for hormone content using immunocytochemistry. This analysis shows that: first, there are cells that are negative for insulin although they were cultured under selective pressure. Second, there is a higher proportion of somatostatin-producing cells than in the parental RIN cells; these somatostatin cells form two populations: one of cells containing only somatostatin and, surprisingly, one made of cells containing both insulin and somatostatin. Thus: (1) expression of the transfected and endogenous insulin regulatory sequences is not regulated in a coordinate fashion; (2) the presence of both hormones in the same cell suggests that the regulation of the expression of insulin and somatostatin genes and the differentiation pathway of the two respective cell types may be closely related.

Secretion of foreign proteins mediated by chicken lysozyme gene regulatory sequences

2002 ◽  
Vol 80 (6) ◽  
pp. 777-788 ◽  
Author(s):  
Gregory R Lampard ◽  
Ann M. Verrinder Gibbins
Keyword(s):  
Fluorescent Protein ◽  
Regulatory Sequences ◽  
Heterologous Proteins ◽  
Transgenic Chicken ◽  
Reporter Protein ◽  
Lysozyme Gene ◽  
Foreign Proteins ◽  
Gene Regulatory ◽  
Chicken Lysozyme

Exploitation of the insulating properties of the complete chicken lysozyme gene domain may facilitate the production of transgenic chicken bioreactors with the capacity to deposit valuable proteins in the egg white. Chimeric genes consisting of the chicken lysozyme gene regulatory sequences and sequences encoding foreign proteins could be inserted randomly into the chicken genome and retain appropriate expression levels. The research reported here established that chicken lysozyme gene regulatory sequences can be used to direct the production and secretion of green fluorescent protein (used as a reporter protein) in transiently transfected chicken blastodermal cells. Attempts to verify these findings in transgenic hens are currently in progress. To provide a rapid means of generating constructs encoding other foreign proteins under the control of lysozyme gene regulatory sequences that can facilitate the secretion of heterologous proteins in vivo, a generic lysozyme gene regulatory scaffold was created using a poxvirus-mediated gene targeting system.Key words: chicken lysozyme gene, secretion, homologous recombination.

scholarly journals A plant mitochondrial sequence transcribed in transgenic tobacco chloroplasts is not edited.

1995 ◽  
Vol 15 (3) ◽  
pp. 1377-1381 ◽  
Author(s):  
C A Sutton ◽  
O V Zoubenko ◽  
M R Hanson ◽  
P Maliga
Keyword(s):  
Transgenic Tobacco ◽  
Rna Editing ◽  
Ribosomal Protein Gene ◽  
Regulatory Sequences ◽  
Mitochondrial Rna ◽  
Higher Plant ◽  
Rna Sequences ◽  
Exon 2 ◽  
Chimeric Genes ◽  
Gene Regulatory

RNA editing occurs in two higher-plant organelles, chloroplasts and mitochondria. Because chloroplasts and mitochondria exhibit some similarity in editing site selection, we investigated whether mitochondrial RNA sequences could be edited in chloroplasts. We produced transgenic tobacco plants that contained chimeric genes in which the second exon of a Petunia hybrida mitochondrial coxII gene was under the control of chloroplast gene regulatory sequences. coxII transcripts accumulated to low or high levels in transgenic chloroplasts containing chimeric genes with the plastid ribosomal protein gene rps16 or the rRNA operon promoter, respectively. Exon 2 of coxII was chosen because it carries seven editing sites and is edited in petunia mitochondria even when located in an abnormal context in an aberrant recombined gene. When editing of the coxII transcripts in transgenic chloroplasts was examined, no RNA editing at any of the usual sites was detected, nor was there any novel editing at any other sites. These results indicate that the RNA editing mechanisms of chloroplasts and mitochondria are not identical but must have at least some organelle-specific components.

scholarly journals Regulatory Sequences of the Mouse Villin Gene That Efficiently Drive Transgenic Expression in Immature and Differentiated Epithelial Cells of Small and Large Intestines

1999 ◽  
Vol 274 (10) ◽  
pp. 6476-6482 ◽  
Author(s):  
Daniel Pinto ◽  
Sylvie Robine ◽  
Frédéric Jaisser ◽  
Fatima El Marjou ◽  
Daniel Louvard
Keyword(s):  
Epithelial Cells ◽  
Regulatory Sequences ◽  
Transgenic Expression

scholarly journals Hypermethylation of calcitonin gene regulatory sequences in human breast cancer as revealed by genomic sequencing

1996 ◽  
Vol 69 (6) ◽  
pp. 471-474 ◽  
Author(s):  
Mika Hakkarainen ◽  
Jarmo Wahlfors ◽  
Sanna Myöhänen ◽  
Mikko O. Hiltunen ◽  
Matti Eskelinen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Genomic Sequencing ◽  
Regulatory Sequences ◽  
Human Breast ◽  
Calcitonin Gene ◽  
Gene Regulatory

Variability in apo(a) gene regulatory sequences, compound genotypes, and association with Lp(a) plasma levels

2007 ◽  
Vol 40 (11) ◽  
pp. 802-805 ◽  
Author(s):  
Kateřina Zídková ◽  
Lukáš Zlatohlávek ◽  
Richard Češka
Keyword(s):  
Plasma Levels ◽  
Regulatory Sequences ◽  
Gene Regulatory
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