scholarly journals Distribution and characteristic of nitrite-dependent anaerobic methane oxidation bacteria in wastewater treatment plants and agriculture fields of northern China

2016 ◽  
Author(s):  
Zhen Hu ◽  
Ru Ma
Keyword(s):  
Wastewater Treatment ◽  
Methane Oxidation ◽  
Paddy Field ◽  
Wastewater Treatment Plants ◽  
Enrichment Culture ◽  
Treatment Plant ◽  
Northern China ◽  
Anaerobic Methane Oxidation ◽  
Corn Field ◽  
Geographical Regions

Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered biological process, which has been arousing global attention because of its potential in minimizing greenhouse gases emissions. In this study, molecular biological techniques and potential n-damo activity batch experiments were conducted to investigate the presence and diversity of M. oxyfera bacteria in paddy field, corn field, and wastewater treatment plant (WWTP) of northern China, as well as lab-scale n-damo enrichment culture. N-damo enrichment culture showed the highest abundance of M. oxyfera bacteria and positive correlation was observed between potential n-damo rate and abundance of M. oxyfera bacteria. Both paddy field and corn field were believed to be better inoculum than WWTP for the enrichment of M. oxyfera bacteria, due to their higher abundance and diversity of M. oxyfera bacteria. Comparative analysis revealed that long biomass retention time and optimum environment (low NH4+ and high NO2- content) were suitable for the growth of M. oxyfera bacteria. In addition, the distribution and diversity of M. oxyfera bacterial might be related to geographical regions.

scholarly journals Distribution and characteristic of nitrite-dependent anaerobic methane oxidation bacteria in wastewater treatment plants and agriculture fields of northern China

2016 ◽  
Author(s):  
Zhen Hu ◽  
Ru Ma
Keyword(s):  
Wastewater Treatment ◽  
Methane Oxidation ◽  
Paddy Field ◽  
Wastewater Treatment Plants ◽  
Enrichment Culture ◽  
Treatment Plant ◽  
Northern China ◽  
Anaerobic Methane Oxidation ◽  
Corn Field ◽  
Geographical Regions

Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered biological process, which has been arousing global attention because of its potential in minimizing greenhouse gases emissions. In this study, molecular biological techniques and potential n-damo activity batch experiments were conducted to investigate the presence and diversity of M. oxyfera bacteria in paddy field, corn field, and wastewater treatment plant (WWTP) of northern China, as well as lab-scale n-damo enrichment culture. N-damo enrichment culture showed the highest abundance of M. oxyfera bacteria and positive correlation was observed between potential n-damo rate and abundance of M. oxyfera bacteria. Both paddy field and corn field were believed to be better inoculum than WWTP for the enrichment of M. oxyfera bacteria, due to their higher abundance and diversity of M. oxyfera bacteria. Comparative analysis revealed that long biomass retention time and optimum environment (low NH4+ and high NO2- content) were suitable for the growth of M. oxyfera bacteria. In addition, the distribution and diversity of M. oxyfera bacterial might be related to geographical regions.

scholarly journals Distribution and characteristic of nitrite-dependent anaerobic methane oxidation bacteria by comparative analysis of wastewater treatment plants and agriculture fields in northern China

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2766 ◽  
Author(s):  
Zhen Hu ◽  
Ru Ma
Keyword(s):  
Wastewater Treatment ◽  
Comparative Analysis ◽  
Methane Oxidation ◽  
Paddy Field ◽  
Wastewater Treatment Plants ◽  
Enrichment Culture ◽  
Treatment Plant ◽  
Northern China ◽  
Anaerobic Methane Oxidation ◽  
Corn Field

Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered biological process which has been arousing global attention because of its potential in minimizing greenhouse gases emissions. In this study, molecular biological techniques and potential n-damo activity batch experiments were conducted to investigate the presence and diversity of M. oxyfera bacteria in paddy field, corn field, and wastewater treatment plant (WWTP) sites in northern China, as well as lab-scale n-damo enrichment culture. N-damo enrichment culture showed the highest abundance of M. oxyfera bacteria, and positive correlation was observed between potential n-damo rate and abundance of M. oxyfera bacteria. Both paddy field and corn field sites were believed to be better inoculum than WWTP for the enrichment of M. oxyfera bacteria due to their higher abundance and the diversity of M. oxyfera bacteria. Comparative analysis revealed that long biomass retention time, low NH${}_{4}^{+}$ and high NO${}_{2}^{-}$ content were suitable for the growth of M. oxyfera bacteria.

scholarly journals Community Composition and Ultrastructure of a Nitrate-Dependent Anaerobic Methane-Oxidizing Enrichment Culture

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Lavinia Gambelli ◽  
Simon Guerrero-Cruz ◽  
Rob J. Mesman ◽  
Geert Cremers ◽  
Mike S. M. Jetten ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Greenhouse Gas ◽  
Methane Oxidation ◽  
Community Composition ◽  
Wastewater Treatment Plants ◽  
Enrichment Culture ◽  
Microbial Interactions ◽  
Anaerobic Methane Oxidation ◽  
Anaerobic Oxidation Of Methane ◽  
Content Type

ABSTRACTMethane is a very potent greenhouse gas and can be oxidized aerobically or anaerobically through microbe-mediated processes, thus decreasing methane emissions in the atmosphere. Using a complementary array of methods, including phylogenetic analysis, physiological experiments, and light and electron microscopy techniques (including electron tomography), we investigated the community composition and ultrastructure of a continuous bioreactor enrichment culture, in which anaerobic oxidation of methane (AOM) was coupled to nitrate reduction. A membrane bioreactor was seeded with AOM biomass and continuously fed with excess methane. After 150 days, the bioreactor reached a daily consumption of 10 mmol nitrate · liter−1· day−1. The biomass consisted of aggregates that were dominated by nitrate-dependent anaerobic methane-oxidizing “CandidatusMethanoperedens”-like archaea (40%) and nitrite-dependent anaerobic methane-oxidizing “CandidatusMethylomirabilis”-like bacteria (50%). The “Ca. Methanoperedens” spp. were identified by fluorescencein situhybridization and immunogold localization of the methyl-coenzyme M reductase (Mcr) enzyme, which was located in the cytoplasm. The “Ca. Methanoperedens” sp. aggregates consisted of slightly irregular coccoid cells (∼1.5-μm diameter) which produced extruding tubular structures and putative cell-to-cell contacts among each other. “Ca. Methylomirabilis” sp. bacteria exhibited the polygonal cell shape typical of this genus. In AOM archaea and bacteria, cytochromecproteins were localized in the cytoplasm and periplasm, respectively, by cytochrome staining. Our results indicate that AOM bacteria and archaea might work closely together in the process of anaerobic methane oxidation, as the bacteria depend on the archaea for nitrite. Future studies will be aimed at elucidating the function of the cell-to-cell interactions in nitrate-dependent AOM.IMPORTANCEMicroorganisms performing nitrate- and nitrite-dependent anaerobic methane oxidation are important in both natural and man-made ecosystems, such as wastewater treatment plants. In both systems, complex microbial interactions take place that are largely unknown. Revealing these microbial interactions would enable us to understand how the oxidation of the important greenhouse gas methane occurs in nature and pave the way for the application of these microbes in wastewater treatment plants. Here, we elucidated the microbial composition, ultrastructure, and physiology of a nitrate-dependent AOM community of archaea and bacteria and describe the cell plan of “Ca. Methanoperedens”-like methanotrophic archaea.

Operational Conditions and Improvement Methods of Municipal Wastewater Treatment Plants under Low Temperatures in Northern China

2010 ◽  
Vol 113-116 ◽  
pp. 651-654
Author(s):  
Ben Chao Jiang ◽  
Fang Ma ◽  
Li Wei ◽  
Jing Bo Guo ◽  
Ang Li
Keyword(s):  
Wastewater Treatment ◽  
Low Temperature ◽  
Low Temperatures ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Northern China ◽  
Municipal Wastewater Treatment ◽  
Operational Conditions ◽  
Municipal Wastewater Treatment Plants

The minimum temperature in winter is under -30oC in the northern hilly regions. It is harmful for the municipal wastewater treatment in winter. According to previous reports, the effects caused by the low-temperature in municipal wastewater treatment plant are summarized, and improvements are put forward. Improving the efficiencies of municipal wastewater treatment is essential and significant in the northern hilly regions.

Analysis and balance of phosphorous removal from wastewater at urban wastewater treatment plant

2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Wastewater Treatment Plants ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Phosphorus Recovery ◽  
Anaerobic Sludge ◽  
Biological Phosphorus Removal ◽  
The Mean

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.

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