scholarly journals Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit treatment

2017 ◽  
Author(s):  
Pan Ning ◽  
Congcong Liu ◽  
Jingquan Kang ◽  
Jinyin Lv
Keyword(s):  
Triticum Aestivum ◽  
Transcription Factors ◽  
Stress Responses ◽  
Segmental Duplication ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Cis Acting ◽  
Group I ◽  
Annotation Information ◽  
Specific Tissue

WRKY proteins, comprising one of the largest transcription factor families in plant kingdom, play crucial roles in the plant development and stress responses. Despite several studies on WRKYs in wheat (Triticum aestivum L.) were investigated, functional annotation information about wheat WRKYs was limited. Here, 171 TaWRKY transcription factors (TFs) were identified from the whole wheat genome and compared with proteins from other 19 species representing nine major plant lineages. A phylogenetic analysis, coupled with gene structure analysis and motif determination, divided these TaWRKYs into seven subgroups (Group I, IIa-e, III). Chromosomal location showed that the most TaWRKY genes were enriched on four chromosomes, especially on chromosome 3B, and 85 (49.7%) genes were either tandem (5) or segmental duplication (80), which suggested that though tandem duplication has contributed to the expansion of TaWRKY family, segmental duplication probably played a more pivotal role. The cis-acting elements analysis revealed putative functions of WRKYs in wheat during development as well as under numerous biotic and abiotic stresses. Finally, the expression of TaWRKY genes in flag leaves, glumes and lemmas under water stress were analyzed, and we found different TaWRKY genes preferentially express in specific tissue during the grain-filling stage. Our results provide a more extensive knowledge on TaWRKYs, which helps to complete the information of WRKY gene family in wheat, and also contribute to screen more candidate genes for further investigation on function characterization of WRKYs under various stresses.

scholarly journals Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit treatment

2017 ◽  
Author(s):  
Pan Ning ◽  
Congcong Liu ◽  
Jingquan Kang ◽  
Jinyin Lv
Keyword(s):  
Triticum Aestivum ◽  
Transcription Factors ◽  
Stress Responses ◽  
Segmental Duplication ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Cis Acting ◽  
Group I ◽  
Annotation Information ◽  
Specific Tissue

WRKY proteins, comprising one of the largest transcription factor families in plant kingdom, play crucial roles in the plant development and stress responses. Despite several studies on WRKYs in wheat (Triticum aestivum L.) were investigated, functional annotation information about wheat WRKYs was limited. Here, 171 TaWRKY transcription factors (TFs) were identified from the whole wheat genome and compared with proteins from other 19 species representing nine major plant lineages. A phylogenetic analysis, coupled with gene structure analysis and motif determination, divided these TaWRKYs into seven subgroups (Group I, IIa-e, III). Chromosomal location showed that the most TaWRKY genes were enriched on four chromosomes, especially on chromosome 3B, and 85 (49.7%) genes were either tandem (5) or segmental duplication (80), which suggested that though tandem duplication has contributed to the expansion of TaWRKY family, segmental duplication probably played a more pivotal role. The cis-acting elements analysis revealed putative functions of WRKYs in wheat during development as well as under numerous biotic and abiotic stresses. Finally, the expression of TaWRKY genes in flag leaves, glumes and lemmas under water stress were analyzed, and we found different TaWRKY genes preferentially express in specific tissue during the grain-filling stage. Our results provide a more extensive knowledge on TaWRKYs, which helps to complete the information of WRKY gene family in wheat, and also contribute to screen more candidate genes for further investigation on function characterization of WRKYs under various stresses.

scholarly journals Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit condition

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3232 ◽  
Author(s):  
Pan Ning ◽  
Congcong Liu ◽  
Jingquan Kang ◽  
Jinyin Lv
Keyword(s):  
Triticum Aestivum ◽  
Water Deficit ◽  
Stress Responses ◽  
Segmental Duplication ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Whole Wheat ◽  
Group I ◽  
Annotation Information ◽  
Specific Tissue

Background WRKY proteins, which comprise one of the largest transcription factor (TF) families in the plant kingdom, play crucial roles in plant development and stress responses. Despite several studies on WRKYs in wheat (Triticum aestivum L.), functional annotation information about wheat WRKYs is limited. Results Here, 171 TaWRKY TFs were identified from the whole wheat genome and compared with proteins from 19 other species representing nine major plant lineages. A phylogenetic analysis, coupled with gene structure analysis and motif determination, divided these TaWRKYs into seven subgroups (Group I, IIa–e, and III). Chromosomal location showed that most TaWRKY genes were enriched on four chromosomes, especially on chromosome 3B. In addition, 85 (49.7%) genes were either tandem (5) or segmental duplication (80), which suggested that though tandem duplication has contributed to the expansion of TaWRKY family, segmental duplication probably played a more pivotal role. Analysis of cis-acting elements revealed putative functions of WRKYs in wheat during development as well as under numerous biotic and abiotic stresses. Finally, the expression of TaWRKY genes in flag leaves, glumes, and lemmas under water-deficit condition were analyzed. Results showed that different TaWRKY genes preferentially express in specific tissue during the grain-filling stage. Conclusion Our results provide a more extensive insight on WRKY gene family in wheat, and also contribute to the screening of more candidate genes for further investigation on function characterization of WRKYs under various stresses.

scholarly journals Variation of 13C and 15N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7738
Author(s):  
Zhaoan Sun ◽  
Shuxia Wu ◽  
Biao Zhu ◽  
Yiwen Zhang ◽  
Roland Bol ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
N Fertilization ◽  
Wheat Growth ◽  
Filling Stage ◽  
N Enrichment ◽  
Two Stages ◽  
Grain Filling Stage

Information on the homogeneity and distribution of 13carbon (13C) and nitrogen (15N) labeling in winter wheat (Triticum aestivum L.) is limited. We conducted a dual labeling experiment to evaluate the variability of 13C and 15N enrichment in aboveground parts of labeled winter wheat plants. Labeling with 13C and 15N was performed on non-nitrogen fertilized (−N) and nitrogen fertilized (+N, 250 kg N ha−1) plants at the elongation and grain filling stages. Aboveground parts of wheat were destructively sampled at 28 days after labeling. As winter wheat growth progressed, δ13C values of wheat ears increased significantly, whereas those of leaves and stems decreased significantly. At the elongation stage, N addition tended to reduce the aboveground δ13C values through dilution of C uptake. At the two stages, upper (newly developed) leaves were more highly enriched with 13C compared with that of lower (aged) leaves. Variability between individual wheat plants and among pots at the grain filling stage was smaller than that at the elongation stage, especially for the −N treatment. Compared with those of 13C labeling, differences in 15N excess between aboveground components (leaves and stems) under 15N labeling conditions were much smaller. We conclude that non-N fertilization and labeling at the grain filling stage may produce more uniformly 13C-labeled wheat materials, whereas the materials were more highly 13C-enriched at the elongation stage, although the δ13C values were more variable. The 15N-enriched straw tissues via urea fertilization were more uniformly labeled at the grain filling stage compared with that at the elongation stage.

scholarly journals Genome-wide identification and expression profiling of glutathione transferase gene family under multiple stresses and hormone treatments in wheat (Triticum aestivum L.)

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Ruibin Wang ◽  
Jingfei Ma ◽  
Qian Zhang ◽  
Chunlai Wu ◽  
Hongyan Zhao ◽  
...  
Keyword(s):  
Stress Responses ◽  
Segmental Duplication ◽  
Expression Profiling ◽  
Abiotic Stresses ◽  
Glutathione Transferase ◽  
Purifying Selection ◽  
Triticum Aestivum L ◽  
Wheat Genome ◽  
Qrt Pcr ◽  
Duplication Events

Abstract Background Glutathione transferases (GSTs), the ancient, ubiquitous and multi-functional proteins, play significant roles in development, metabolism as well as abiotic and biotic stress responses in plants. Wheat is one of the most important crops, but the functions of GST genes in wheat were less studied. Results A total of 330 TaGST genes were identified from the wheat genome and named according to the nomenclature of rice and Arabidopsis GST genes. They were classified into eight classes based on the phylogenetic relationship among wheat, rice, and Arabidopsis, and their gene structure and conserved motif were similar in the same phylogenetic class. The 43 and 171 gene pairs were identified as tandem and segmental duplication genes respectively, and the Ka/Ks ratios of tandem and segmental duplication TaGST genes were less than 1 except segmental duplication gene pair TaGSTU24/TaGSTU154. The 59 TaGST genes were identified to have syntenic relationships with 28 OsGST genes. The expression profiling involved in 15 tissues and biotic and abiotic stresses suggested the different expression and response patterns of the TaGST genes. Furthermore, the qRT-PCR data showed that GST could response to abiotic stresses and hormones extensively in wheat. Conclusions In this study, a large GST family with 330 members was identified from the wheat genome. Duplication events containing tandem and segmental duplication contributed to the expansion of TaGST family, and duplication genes might undergo extensive purifying selection. The expression profiling and cis-elements in promoter region of 330 TaGST genes implied their roles in growth and development as well as adaption to stressful environments. The qRT-PCR data of 14 TaGST genes revealed that they could respond to different abiotic stresses and hormones, especially salt stress and abscisic acid. In conclusion, this study contributed to the further functional analysis of GST genes family in wheat.

Water use of some recent bread and durum wheat cultivars in western Canada

2007 ◽  
Vol 87 (2) ◽  
pp. 289-292 ◽  
Author(s):  
H. Wang ◽  
T. N. McCaig ◽  
R. M. DePauw ◽  
J. M. Clarke ◽  
R. Lemke
Keyword(s):  
Triticum Aestivum ◽  
Drought Tolerance ◽  
Soil Water ◽  
Water Use ◽  
Triticum Turgidum ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Western Canada ◽  
Wheat Cultivars ◽  
Use Efficiency

Recently developed cultivars of Canada Western Red Spring (CWRS) wheat (Triticum aestivum L.) and Canada Western Amber Durum (CWAD) (Triticum turgidum L. var durum) produced significantly more grain than older cultivars. This production was attributed to higher harvest indices and better water use efficiency. Durum cultivars and CWRS AC Intrepid and AC Barrie extracted relatively more soil water below 55 cm, which may be advantageous in minimizing leaching and related to drought tolerance during grain-filling. Key words: Hexaploid wheat, durum, water use, soil water

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