scholarly journals Blood, sweat and tears: non-invasive vs. non-disruptive DNA sampling for experimental biology

Author(s):  
Marie-Caroline Lefort ◽  
Stephane Boyer ◽  
Arijana Barun ◽  
Arsalan Emami Khoyi ◽  
Johnathon Ridden ◽  
...  

DNA data are becoming increasingly important in experimental biology. For example, it may be necessary to obtain DNA from an organism before using it in a bioassay or an experiment, to identify and distinguish between cryptic species, or when comparing different morphocryptic genotypes. Another example could be the assessment of relatedness between organisms prior to a behavioural study. In such cases, it is important to obtain DNA without affecting the fitness or behaviour of the subject being tested, as this could bias the results of the experiment. This points out the existence of a gap in the current molecular and experimental biology terminology, for which we propose the use of the term non-disruptive DNA sampling, specifically addressing behaviour and/or fitness, rather than simply physical integrity (invasiveness). We refer to these methods as “non-disruptive”, and discuss when they are appropriate to use.

Author(s):  
Marie-Caroline Lefort ◽  
Stephane Boyer ◽  
Arijana Barun ◽  
Arsalan Emami Khoyi ◽  
Johnathon Ridden ◽  
...  

DNA data are becoming increasingly important in experimental biology. For example, it may be necessary to obtain DNA from an organism before using it in a bioassay or an experiment, to identify and distinguish between cryptic species, or when comparing different morphocryptic genotypes. Another example could be the assessment of relatedness between organisms prior to a behavioural study. In such cases, DNA must be obtained without affecting the fitness or behaviour of the subject being tested, as this could bias the results of the experiment. This points out the existence of a gap in the current molecular and experimental biology terminology, for which we propose the use of the term non-disruptive DNA sampling, specifically addressing behaviour and/or fitness, rather than simply physical integrity (invasiveness). We refer to these methods as “non-disruptive”, and discuss when they are appropriate to use.


2015 ◽  
Author(s):  
Marie-Caroline Lefort ◽  
Stephane Boyer ◽  
Arijana Barun ◽  
Arsalan Emami Khoyi ◽  
Johnathon Ridden ◽  
...  

DNA data are becoming increasingly important in experimental biology. For example, it may be necessary to obtain DNA from an organism before using it in a bioassay or an experiment, to identify and distinguish between cryptic species, or when comparing different morphocryptic genotypes. Another example could be the assessment of relatedness between organisms prior to a behavioural study. In such cases, DNA must be obtained without affecting the fitness or behaviour of the subject being tested, as this could bias the results of the experiment. This points out the existence of a gap in the current molecular and experimental biology terminology, for which we propose the use of the term non-disruptive DNA sampling, specifically addressing behaviour and/or fitness, rather than simply physical integrity (invasiveness). We refer to these methods as “non-disruptive”, and discuss when they are appropriate to use.


Author(s):  
Marie-Caroline Lefort ◽  
Stephane Boyer ◽  
Arijana Barun ◽  
Arsalan Emami Khoyi ◽  
Johnathon Ridden ◽  
...  

DNA data are becoming increasingly important in experimental biology. For example, it may be necessary to obtain DNA from an organism before using it in a bioassay or an experiment, to identify and distinguish between cryptic species, or when comparing different morphocryptic genotypes. Another example could be the assessment of relatedness between organisms prior to a behavioural study. In such cases, DNA must be obtained without affecting the fitness or behaviour of the subject being tested, as this could bias the results of the experiment. This points out the existence of a gap in the current molecular and experimental biology terminology, for which we propose the use of the term non-disruptive DNA sampling, specifically addressing behaviour and/or fitness, rather than simply physical integrity (invasiveness). We refer to these methods as “non-disruptive”, and discuss when they are appropriate to use.


2018 ◽  
Author(s):  
M.C. Lefort ◽  
R.H. Cruickshank ◽  
K. Descovich ◽  
N.J. Adams ◽  
A. Barun ◽  
...  

AbstractThe use of DNA data is ubiquitous across animal sciences. DNA may be obtained from an organism for a myriad of reasons including identification and distinction between cryptic species, sex identification, comparisons of different morphocryptic genotypes or assessments of relatedness between organisms prior to a behavioural study. DNA should be obtained while minimizing the impact on the fitness, behaviour or welfare of the subject being tested, as this can bias experimental results and cause long-lasting effects on wild animals. Furthermore, minimizing impact on experimental animals is a key Refinement principle within the ‘3Rs’ framework which aims to ensure that animal welfare during experimentation is optimised. The term ‘non-invasive DNA sampling’ has been defined to indicate collection methods that do not require capture or cause disturbance to the animal, including any effects on behaviour or fitness. In practice this is not always the case, as the term ‘non-invasive’ is commonly used in the literature to describe studies where animals are restrained or subjected to aversive procedures. We reviewed the non-invasive DNA sampling literature for the past six years (380 papers published in 2013-2018) and uncovered the existence of a significant gap between the current use of this terminology (i.e. ‘non-invasive DNA sampling’) and its original definition. We show that 58% of the reviewed papers did not comply with the original definition. We discuss the main experimental and ethical issues surrounding the potential confusion or misuse of the phrase ‘non-invasive DNA sampling’ in the current literature and provide potential solutions. In addition, we introduce the terms ‘non-disruptive’ and ‘minimally disruptive’ DNA sampling, to indicate methods that eliminate or minimise impacts not on the physical integrity/structure of the animal, but on its behaviour, fitness and welfare, which in the literature reviewed corresponds to the situation for which an accurate term is clearly missing. Furthermore, we outline when these methods are appropriate to use.


2020 ◽  
Author(s):  
Małgorzata Cieślak-Kopyt ◽  
Dorota Pogodzińska

The subject of the monograph, published as the 10th volume of the Saved Archaeological Heritage series, are the results of rescue excavations on a cemetery from the period of Roman influence on the Vistula River near Magnuszew in southern Mazovia (Poland), carried out several years ago at the initiative of the Museum in Radom. This necropolis, like many similar ones throughout the country, was systematically destroyed as a result of agricultural activities, and in recent years also through illegal prospection with the use of metal detectors. Archaeologists, with the cooperation of numerous volunteers, managed to protect against further destruction about 60 graves (urned and urnless) from the period between the 1st century BC and the 3rd century CE. These are an evidence of the settlement of the region by people whose material traces are referred to in the archaeological nomenclature as the Przeworsk culture (associated mainly with the Germanic tribes). The cinerary graves were equipped with ceramics, metal parts of clothing, tools, less often weapons, glass beads, imported vessels or dice. Among the forms of graves, the so-called groove object stands out: a kind of rectangular grave feature tied with survival to the beginnings of our era of Celtic traditions, arriving here from northern Małopolska. In addition to the standard catalogue with the description of graves, pottery and small finds, and very detailed illustration plates, the monograph includes an analysis of material culture and forms of burial, photographs of selected finds and very extensive specialist reports. The latter include both osteological materials (anatomo-anthropological analysis, analysis of animal bones placed in the graves), as well as other ecofacts and individual categories of furnishings (glass, faience, iron and bronze objects). The whole is complemented by clear plans with the location of graves and artifacts in the necropolises, as well as with the results of non-invasive research going far beyond the excavated area and of key importance for further in situ protection of this extremely valuable monument.


2018 ◽  
Vol 13 (5) ◽  
pp. 42 ◽  
Author(s):  
R. Brady ◽  
D.O. Frank-Ito ◽  
H.T. Tran ◽  
S. Janum ◽  
K. Møller ◽  
...  

The objective of this study was to develop a personalized inflammatory model and estimate subject-specific parameters that could be related to changes in heart rate variability (HRV), a measure that can be obtained non-invasively in real time. An inflammatory model was developed and calibrated to measurements of interleukin-6 (IL-6), tumor necrosis factor (TNF-alpha), interleukin-8 (IL-8) and interleukin-10 (IL-10) over 8 hours in 20 subjects administered a low dose of lipopolysaccharide. For this model, we estimated 11 subject-specific parameters for all 20 subjects. Estimated parameters were correlated with changes in HRV, computed from ECG measurements using a built-in HRV module available in Labchart. Results revealed that patients could be separated into two groups expressing normal and abnormal responses to endotoxin. Abnormal responders exhibited increased HRV, most likely as a result of increased vagal firing. The observed correlation between the inflammatory response and HRV brings us a step further towards understanding if HRV predictions can be used as a marker for inflammation. Analyzing HRV parameters provides an easy, non-invasively obtained measure that can be used to assess the state of the subject, potentially translating to identifying a non-invasive marker that can be used to detect the onset of sepsis.


2016 ◽  
Vol 61 (4) ◽  
pp. 299-307 ◽  
Author(s):  
L. M. Kubasiewicz ◽  
J. Minderman ◽  
L. C. Woodall ◽  
C. P. Quine ◽  
R. Coope ◽  
...  

2019 ◽  
Vol 9 (21) ◽  
pp. 4561
Author(s):  
Shin ◽  
Ryu ◽  
Cho ◽  
Yang ◽  
Lee

Although non-invasive brain stimulation techniques do not involve surgical procedures, the challenge remains in correctly locating the stimulator from outside the head. There is a limit to which one can manually and precisely position and orient the stimulator or repeatedly move the stimulator around the same position. Therefore, in this study, we developed a serial robot with 6 degrees-of-freedom to move the stimulator and a neuro-navigation system to determine the stimulus point from looking at the shape of the subject’s brain. The proposed robot applied a spherical mechanism while considering the safety of the subject, and the workspace of the robot was designed considering the shape of the human head. Position-based visual servoing was applied to compensate for unexpected movements during subject stimulation. We also developed a neuro-navigation system that allows us visually to check the focus of the stimulator and the human brain at the same time and command the robot to the desired point. To verify the system performance, we first performed repeatability and motion compensation experiments of the robot and then evaluated the repeated biosignal response experiments through transcranial magnetic stimulation, a representative technique of non-invasive brain stimulation.


2014 ◽  
Vol 6 (1) ◽  
Author(s):  
A. Rossi ◽  
G. Sprugnoli ◽  
E. Santarnecchi

Human mind can follow two opposite types of reasoning in everyday life as well as in science: convergent thinking, as the ability to get the unique solution to a problem, or divergent thinking, the ability to elaborate different answer to a question. The latter is usually considered as an essential feature of the “creative mind”, together with Insight, an unpredictable and unexpected moment of exceptional thinking commonly reported as the “Eureka!" experience. During such processing, an unconscious reorganization process of previously unrelated problem elements is made and when the solution finally emerges to consciousness, the subject is not able to explain how he/she reached it. Because of its unpredictable and unconscious nature, as well as its connection with creativity and scientific discoveries, the definition and evaluation of insight is now one of the biggest challenges for modern cognitive neuroscience. Neurophysiological evidence begins to arise, making the enhancement of creativity thinking using non-invasive neuromodulation techniques a plausible future scenario.


2020 ◽  
Vol 34 (4) ◽  
pp. 633-644
Author(s):  
Gergely Balázs ◽  
Judit Vörös ◽  
Brian Lewarne ◽  
Gábor Herczeg

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