scholarly journals Three dimensional reconstructions of Nummulites tests reveal complex chamber shapes

Author(s):  
Willem Renema ◽  
Laura Cotton

Larger benthic foraminifera (LBF) are an important tool for the biostratigraphy of (sub)tropical shallow marine deposits. In Paleogene the genus Nummulites is an important genus for biostratigraphical zonation schemes. However, classification is Europe centered and based on external characters and equatorial thin sections. New results from regions outside the northern Tethys shows that a more rigid framework for the classification of nummulites is needed. Here we present a new tool for achieving this goal. We visualise 3D chamber shape of Nummulites djodjokartae and compare these to traditional morphometrical characters. To achieve this goal we use computed microtomography of well preserved Nummulites tests. We find that despite the regular shape in equatorial and axial thin section 3D chamber shape is not predicted by these sections. We argue that 3D reconstructions of Nummulites tests will be a great aid in improving our understanding lineages within the genus Nummulites, and to elucidate its evolutionary and biogeographical history.

2015 ◽  
Author(s):  
Willem Renema ◽  
Laura Cotton

Larger benthic foraminifera (LBF) are an important tool for the biostratigraphy of (sub)tropical shallow marine deposits. In Paleogene the genus Nummulites is an important genus for biostratigraphical zonation schemes. However, classification is Europe centered and based on external characters and equatorial thin sections. New results from regions outside the northern Tethys shows that a more rigid framework for the classification of nummulites is needed. Here we present a new tool for achieving this goal. We visualise 3D chamber shape of Nummulites djodjokartae and compare these to traditional morphometrical characters. To achieve this goal we use computed microtomography of well preserved Nummulites tests. We find that despite the regular shape in equatorial and axial thin section 3D chamber shape is not predicted by these sections. We argue that 3D reconstructions of Nummulites tests will be a great aid in improving our understanding lineages within the genus Nummulites, and to elucidate its evolutionary and biogeographical history.


2021 ◽  
Author(s):  
Vlasta Ćosović ◽  
Jelena Španiček ◽  
Katica Drobne ◽  
Ervin Mrinjek

<p>The Paleogene Adriatic carbonate platform(s) existed within the Central NeoTethys (around 32 N paleolatitude) from the Danian to the late Eocene (Bartonian/Priabonian) and produced a succession of limestones up to 500 m thick, rich in larger benthic foraminifera (LBF). The Eocene sediments are widely distributed along the eastern Adriatic coast and have been studied for many years. Taking into account the climatic changes that took place within the Eocene (Early Eocene and Middle Eocene climatic optima, known as EECO, MECO), special attention was paid to the composition of shallow-marine foraminiferal assemblages. The studies reveal the following trends: (1) the alveolinid-dominated assemblages were replaced by nummulitid-dominated assemblages around the MECO; (2) the greater species and morphological diversity (spherical, ellipsoid, extremely elongated fusiform) of the alveolinid fauna was evident at the EECO; (3) the nummulitid-dominated fauna was characterized by less diversified assemblages compared to the alveolinid ones and by the co-occurrence of scleractinian corals, coralline red algae and aborescent foraminifera. The occurrence of twin embryos has been assigned to the early Eocene in the alveolinid populations, especially in Alveolina levantina and A. axiampla (in some sections, the frequency is greater than 5%), and these coalesced embryos have the same size as the single form (usually they are smaller). The LBF assemblages of Middle Eocene showed a greater frequency of doubled adult tests (Orbitolites sp., Nummulites sp.). The origin of these unusual morphologies is poorly known, usually described as the results of stressful conditions. Considering the timing of the appearance of such morphologies, temperature and associated changes in the shallow-marine environment could be the cause.</p><p>This study is carried out as part of the scientific project IP-2019-04-5775 BREEMECO, funded by Croatian Scientific Foundation.</p>


2021 ◽  
pp. 1-21
Author(s):  
Cemile Solak ◽  
Kemal Taslı ◽  
Hayati Koç

Abstract Cretaceous carbonates in the Geyik Dağı area (Central Taurides, southern Turkey) are represented by two successions with different paleoenvironmental settings: open shelf to slope succession of Cenomanian to Danian age and inner platform succession of Albian to Maastrichtian age, which is interrupted by a post-Cenomanian disconformity. Outcropped lowermost part of the platform-type one is composed of rudistid limestones corresponding to the Urgonian-type carbonates and belongs to the Geyik Dağı Unit (=Anamas-Akseki Carbonate Platform). It contains a rich assemblage of larger benthic foraminifera including orbitolinid, chrysalidinid, cuneolinid, nezzazatid, and miliolid taxa, which has been illustrated and documented here for the first time from the upper Albian of the Tauride Carbonate Platform. The occurrence of such a diversified foraminiferal fauna indicates a prominent high diversity that took place in the Tauride Carbonate Platform during the late Albian time, which corresponds to a major emersion period in some parts of the platform.


2021 ◽  
Vol 91 (7) ◽  
pp. 773-794
Author(s):  
Bassam Alshammari ◽  
Nigel P. Mountney ◽  
Luca Colombera ◽  
Mohammed A. Al-Masrahy

ABSTRACT The interaction of fluvial, tidal, and wave processes in coastal and paralic environments gives rise to sedimentary successions with highly varied styles of facies architecture; these are determined by the morphology and evolutionary behavior of the range of coastal sub-environments, which may be difficult to diagnose in subsurface sedimentary successions with limited well control. This study presents depositional models to account for stratigraphic complexity in a subsurface fluvial to shallow-marine succession, the Middle Jurassic Dhruma Formation, Saudi Arabia. The study achieves the following: i) it examines and demonstrates sedimentary relationships between various fluvial, nearshore, and shallow-marine deposits, ii) it develops depositional models to account for the stratigraphic complexity inherent in fluvial to shallow-marine successions, and iii) it documents the sedimentology and the stratigraphic evolutionary patterns of the lower Dhruma Formation in the studied area of Saudi Arabia. The dataset comprises facies descriptions of 570 m of core from 14 wells, 77 representative core thin sections, 14 gamma-ray logs, and FMI image logs from 4 wells. These data are integrated with quantitative information from > 50 analogous systems from a wide range of modern and ancient settings, stored in a relational database. Stratigraphic correlations reveal the internal anatomy of the succession. Facies associations are representative of fluvial channels, intertidal flats, pedogenically modified supratidal flats or floodplains, river-influenced tidal bars, weakly storm-affected shoreface and offshore-transition zones, storm-dominated delta-front and prodelta settings, and an open-marine carbonate-dominated shelf. These sub-environments interacted in a complex way through space and time. The vertical succession of the studied interval records an overall transition from coastal-plain deposits at the base to marine deposits at the top. As such, the succession records a long-term transgressive, deepening-upward trend. However, this general trend is punctuated by repeated progradational events whereby coastal sand bodies of fluvial, wave, and tidal origin prograded basinward during stillstands to fill bays along a coastline. The nature of juxtaposition of neighboring sub-environments has resulted in a sedimentary record that is highly complex compared to that generated by morphologically simple shoreface systems that accumulate more regularly ordered stratal packages.


2016 ◽  
Vol 22 (1) ◽  
pp. 16
Author(s):  
Luli Gustiantini ◽  
Kresna Tri Dewi ◽  
Anne Muller ◽  
Praptisih Praptisih

A 30m-long sediment core covering the Holocene period was taken from the area of Gombong in the southern part of Central Java. The sediments were deposited in a shallow marine to lagoonal environment that was confirmed by the dominance of Ammonia beccarii along the core intervals. In addition, the species Quinqueloculina poeyana, Miliolinella lakemacquariensis, and Miliolinella subrotunda were also found in the sediments that are typical of normal shallow marine conditions. The decrease and increase in the abundance of these species throughout the core is an expression of sea level change in the area, which results the environmental changes. Low sea level is expressed by the dominance of Ammonia beccarii, and the low abundances or absence of the other three species. In contrast, high sea level stands are reflected by the presence of all four species. The high sea level would imply favorable conditions for benthic foraminifera because it would result in normal shallow marine conditions in the area. Finally, from this benthic assemblages study, it can be assumed that the environmental transformation from the originally shallow marine environment into land was occurred at level 5.5m depths of the sediment core, when all benthic foraminifera were terminated, including Ammonia beccarii. These new results from the shallow marine deposits in the Gombong area are a new contribution to the understanding of paleoenvironmental change in the region, which in turn is important for understanding sea level change as well as climate change in the region. Keywords: Benthic foraminifera, Holocene, paleoenvironmental changes, sea level changes Southcoast of Central Java Sebuah percontoh sedimen bor sepanjang 30m yang berumur Holosen diambil dari daerah Gombong, bagian selatan Jawa Tengah. Percontoh sedimen diendapkan pada lingkungan laut dangkal –laguna, berdasarkan kelimpahan foraminifera bentik Ammonia beccarii di sepanjang sedimen bor. Selain itu ditemukan juga spesies-spesies Quinqueloculina poeyana, Miliolinella lakemacquariensis, dan Miliolinella subrotunda, yang merupakan penciri lingkungan laut dangkal dengan kondisi normal. Penurunan dan kenaikan dari kelimpahan masing-masing spesies foraminifera bentik di atas, dapat mencerminkan perubahan permukaan air laut daerah studi, yang menghasilkan terjadinya perubahan lingkungan. Penurunan muka air laut dapat dicirikan dengan hadirnya Ammonia beccarii yang sangat dominan, sementara spesies lainnya cenderung berkurang bahkan hampir tidak ada. Sebaliknya ketika muka air laut naik, maka keempat spesies foraminifera tersebut cenderung hadir dengan jumlah yang seimbang satu sama lainnya. Kenaikan muka air laut akan menghasilkan lingkungan laut normal yang merupakan kondisi ideal bagi foraminifera. Akhirnya, dari kajian perubahan kelimpahan foraminifera bentik ini, dapat diperkirakan bahwa pada level kedalaman bor 5,5m, terjadi perubahan lingkungan dari lingkungan laut dangkal-laguna menjadi daratan, yang ditandai dengan musnahnya semua jenis foraminifera bentik, termasuk Ammonia beccarii. HAsil kajian ini merupakan kontribusi baru untuk mempelajari perubahan lingkungan pada lokasi penelitian, terutama penting untuk lebih mengerti mengenai perubahan muka air laut dan perubahan iklim. Keywords: Benthic foraminifera, Holocene, paleoenvironmental changes, sea level changes


Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.


Author(s):  
S. Cusack ◽  
J.-C. Jésior

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).


Author(s):  
R. P. Becker ◽  
J. J. Wolosewick ◽  
J. Ross-Stanton

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.


Author(s):  
Jerome J. Paulin

Within the past decade it has become apparent that HVEM offers the biologist a means to explore the three-dimensional structure of cells and/or organelles. Stereo-imaging of thick sections (e.g. 0.25-10 μm) not only reveals anatomical features of cellular components, but also reduces errors of interpretation associated with overlap of structures seen in thick sections. Concomitant with stereo-imaging techniques conventional serial Sectioning methods developed with thin sections have been adopted to serial thick sections (≥ 0.25 μm). Three-dimensional reconstructions of the chondriome of several species of trypanosomatid flagellates have been made from tracings of mitochondrial profiles on cellulose acetate sheets. The sheets are flooded with acetone, gluing them together, and the model sawed from the composite and redrawn.The extensive mitochondrial reticulum can be seen in consecutive thick sections of (0.25 μm thick) Crithidia fasciculata (Figs. 1-2). Profiles of the mitochondrion are distinguishable from the anterior apex of the cell (small arrow, Fig. 1) to the posterior pole (small arrow, Fig. 2).


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