scholarly journals Distilled liquid smoke coconut shell attenuates the cytokine profile of macrophages in oral ulcer in experimental model of diabetes mellitus

2021 ◽  
Author(s):  
Surboyo Meircurius Dwi Condro ◽  
Ernawati Diah Savitri ◽  
Radithia Desiana ◽  
Soebadi Bagus ◽  
Mahdani Fatma Yasmin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Model ◽  
Cytokine Profile ◽  
Oral Ulcer ◽  
Coconut Shell ◽  
Liquid Smoke

scholarly journals Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell

2017 ◽  
Vol 50 (2) ◽  
pp. 71
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Ira Arundina ◽  
Retno Pudji Rahayu
Keyword(s):  
Diabetes Mellitus ◽  
Topical Application ◽  
Wistar Rats ◽  
Healing Process ◽  
Coconut Shell ◽  
Diabetic Patients ◽  
Process Indicator ◽  
Liquid Smoke ◽  
Patients With Diabetes ◽  
Male Wistar Rats

Background: Traumatic ulcers in patients with diabetes mellitus may experience delayed healing despite their diabetic condition being controlled. Liquid smoke coconut shell containing phenolic compounds can potentially accelerate the healing process. One healing process indicator is the increased number of fibroblasts, another being the increased amount of collagen. Purpose: This study aimed to analyze the amount of collagen in traumatic ulcers in diabetics after application of liquid smoke coconut shell. Methods: Alloxan was induced in twenty-four male Wistar rats as models of diabetes mellitus. A traumatic 10 mm ulcer was made along the labial fornix incisive inferior with a round, stainless steel blade before liquid smoke coconut shell and benzydamine hydrochloride (as the control) was administrated once a day. A biopsy of the labial fornix incisive inferior was subsequently performed after the topical application for 5 and 7 days. Histological assessment was conducted to analyze the amount of collagen by means of Masson Trichome staining. Results: Histologically, the topical application of liquid smoke coconut shell for 5 days significantly increased the amount of collagen, higher than that of benzydamine hydrochloride as the control (p=0.006) (p<0.05). Meanwhile, the topical application of liquid smoke coconut shell for 7 days made the concentration of collagen no significantly different from that of benzydamine hydrochloride as the control (p=0.156) (p>0.05). Conclusion: Liquid smoke coconut shell applied for 5 days increase the amount of collagen in traumatic ulcers in diabetic patients.

scholarly journals The cytotoxicity, anti-inflammation, anti-nociceptive and oral ulcer healing properties of coconut shell liquid smoke

2021 ◽  
Vol 10 (4) ◽  
pp. 459-467
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Fatma Yasmin Mahdani ◽  
Nurina Febriyanti Ayuningtyas ◽  
Arvind Babu Rajendran Santosh ◽  
Diah Savitri Ernawati ◽  
...  
Keyword(s):  
Ulcer Healing ◽  
Cocos Nucifera ◽  
Oral Ulcer ◽  
Coconut Shell ◽  
Gas Chromatography Mass Spectrometry ◽  
Hot Plate Test ◽  
Liquid Smoke ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Anti Inflammation

Introduction: Coconut shell liquid smoke (CS-LS) from Cocos nucifera L. has been traditionally used by Indonesians as a natural preservative. Besides that, liquid smoke is also used as a medicine to treat various types of wounds. During the storage, liquid smoke resulting from pyrolysis is still questionable in relation to the oxidation process and changes in its properties and potentials. We observed the physical characteristics, components, toxicity, anti-inflammatory, anti-nociceptive properties, and effect in oral ulcer healing of CS-LS. Methods: Acidity was analyzed using a digital pH meter, density test was analyzed using a pycnometer, and the components were determined using gas chromatography-mass spectrometry (GC-MS). Eight concentrations of CS-LS (1%, 2%, 4%, 6%, 8%, 10%, 12%, and 14%) were tested on baby hamster kidney (BHK21) for the extract toxicity, carrageenan-induced rat paw edema for its anti-inflammatory properties, hot-plate test for its anti-nociceptive, and traumatized labial fornix incisive inferior for its oral ulcer healing. Results: The acidity of CS-LS was 2.296 and the density was 1.0102 g/mL. The major components analyzed were phenol (32.75%), 2-methoxy-phenol (17.45%), and furfural (13.09%). The CS-LS 100% and CS-LS 8% were the optimum concentrations for maintaining the BHK21 and increasing the number of fibroblasts in oral ulcer healing. The CS-LS 100% showed potent anti-nociceptive ability compared to other concentrations (P = 0.001), but not for the anti-inflammation properties. Conclusion: CS-LS is a promising natural herb for oral medicine, especially oral ulcer medicine.

scholarly journals RANCANG BANGUN MESIN PRODUKSI ASAP CAIR DARI TEMPURUNG KELAPA BERBASIS TEKNOLOGI CYCLONE-REDISTILLATION

2019 ◽  
Vol 7 (2) ◽  
pp. 148-156 ◽  
Author(s):  
Muhammad Fathussalam ◽  
Angky Wahyu Putranto ◽  
Bambang Dwi Argo ◽  
Arta Harianti ◽  
Arsyika Oktaviani ◽  
...  
Keyword(s):  
Coconut Shell ◽  
Liquid Smoke

Tingginya produksi tanaman kelapa di Indonesia mempunyai hubungan yang postif dengan jumlah limbah tempurung kelapa yang dihasilkan, dimana limbah tersebut cukup sulit diuraikan oleh mikroorganisme. Sehingga pemanfaatan limbah tempurung kelapa menjadi arang dan asap cair telah banyak dikembangkan dalam beberapa tahun terakhir. Selama ini, proses pembuatan asap cair dari tempurung kelapa (coconut shell-liquid smoke) dilakukan secara konvensional selama 3-5 hari dengan kualitas asap cair grade C dan rendemen asap cair yang rendah. Oleh karena itu tujuan penelitian ini yaitu melakukan rancang bangun mesin pembuat asap cair dari tempurung kelapa berbasis teknologi cyclone-redistillation yang dapat meningkatkan rendemen dari asap cair yang dihasilkan. Pengujian performansi mesin dianisis berdasarkan parameter rendemen dari setiap grade (A, B dan C) yang dihasilkan. Metode yang digunakan dalam penelitian ini adalah metode rekayasa (engineering). Hasil penelitian menunjukkan bahwa mesin pembuat asap cair dari tempurung kelapa berbasis teknologi cyclone-redistillation telah diproduksi sesuai spesifikasi dengan dimensi keseluruhan panjang 132 cm, lebar 100 cm dan tinggi 145 cm. Rendemen asap cair tempurung kelapa grade A sebesar 2,17%, sedangkan grade B dan C masing-masing memiliki rendemen 0,33% dan 0,83%. Rancang bangun mesin pembuat asap cair dari tempurung kelapa berbasis teknologi cyclone-redistillation ini berpotensi besar untuk diterapkan pada UKM penghasil asap cair di Indonesia untuk meningkatkan produktivitas dan kualitas asap cair dari limbah tempurung kelapa. 

scholarly journals The cyclone separator application on physicochemical characterization of coconut shell-liquid smoke grade C

2020 ◽  
Vol 3 (2) ◽  
pp. 68-74
Author(s):  
Angky Wahyu Putranto ◽  
Firda Puspaningarum ◽  
Sukardi Sukardi
Keyword(s):  
Total Yield ◽  
Physicochemical Characterization ◽  
Common Method ◽  
Coconut Shell ◽  
Physical Parameters ◽  
Cyclone Separator ◽  
Liquid Smoke ◽  
Direct Condensation

The further processing of coconut shell pyrolysis into liquid smoke has been developed in recent years. However, the common technology by directly condensing the smoke has many impurity compounds which decreases the yield of liquid smoke produced. Hence, in this study the cyclone separator was applied to increase the quality of physicochemical content in coconut shell-liquid smoke (CS-LS) grade C. The physical parameters analyzed were yield, pH, density and color. The chemical parameters was analyzed by GC-MS. The result showed that CS-LS processing with cyclone separator was able to increase the total yield into 3.33%, with better color, pH and density compared to the CS-LS produced using common method (direct condensation). The application of cyclone separator was also able to increase the phenolic compounds (97%), alcoholic compounds (92%), cycloalkene compounds (91%) and also decreased the benzoic acid, carbonyl and ester up to 100% compared with common method for CS-LS grade C production.

scholarly journals Chemical Identification of Coconut Shell Liquid Smoke

2019 ◽  
Vol 309 ◽  
pp. 012020
Author(s):  
I Mulyawanti ◽  
S I Kailaku ◽  
A N A Syah ◽  
Risfaheri
Keyword(s):  
Coconut Shell ◽  
Chemical Identification ◽  
Liquid Smoke

FP488EFFECT OF CONVERSION FROM TACROLIMUS TO CTLA4IG IN EXPERIMENTAL MODEL OF DIABETES MELLITUS

2019 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Kang Luo ◽  
Lim Sun Woo ◽  
Y I QUAN ◽  
Sheng Cui ◽  
Jin Shin Yoo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Model

scholarly journals Potential of Distilled Liquid Smoke Derived from Coconut (Cocos nucifera L) Shell for Traumatic Ulcer Healing in Diabetic Rats

2019 ◽  
Vol 13 (02) ◽  
pp. 271-279 ◽  
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Ira Arundina ◽  
Retno Pudji Rahayu ◽  
Dieni Mansur ◽  
Taufan Bramantoro
Keyword(s):  
Ulcer Healing ◽  
Cocos Nucifera ◽  
Coconut Shell ◽  
Diabetic Rat ◽  
Natural Food ◽  
Necrosis Factor Alpha ◽  
Liquid Smoke ◽  
Tnf Α ◽  
Significant Difference ◽  
Cocos Nucifera L

Abstract Objective Distilled liquid smoke (DLS) is a result of coconut processing by-product that not only serves as a natural food preservative but also has a promising therapeutic effect. The healing potential of DLS derived from coconut (Cocos nucifera L) shell was investigated on a traumatic ulcer with the diabetic rat. Materials and Methods DLS was analyzed the component by gas chromatograph mass spectrometry. Diabetic condition was induced by alloxan in 55 male Wistar rats. Ten mm of traumatic ulcer was made along the labial fornix incisive inferior after the diabetic condition was confirmed. Then DLS coconut shell, benzydamine hydrochloride, and sterile distilled water were applied topically for 3, 5, and 7 days. The potential healing was evaluated based on the expression of nuclear factor kappa beta (NFκB) and tumor necrosis factor alpha (TNF-α) on macrophages using immunohistochemical staining and the amount of collagen using Masson Trichome staining. The difference between each group was analyzed using one-way analysis of variance. The least significant difference test is used to determine the significant difference (p < 0.05). Results The major compounds found were phenol (36.6%), 2-methoxyphenol (guaiacol) (25.2%), furfural (17.8%), and 4-ethyl-2-methoxyphenol (3.5%) with 28 other minor constituents. The lowest NFκB and TNF-α expression on macrophage was observed by topical application of DLS derived from coconut shell for 3, 5, and 7 days of treatment. The amount of collagen was increased and indicated by the highest result of DLS compared to others. Conclusion The DLS derived from coconut (Cocos nucifera L) shell was able to improve traumatic ulcer healing in a person with diabetes.

Streptozotocin-induced renal hemodynamic changes in isogenic Lewis rats: a kidney transplant study

1993 ◽  
Vol 264 (1) ◽  
pp. F100-F105 ◽  
Author(s):  
P. Churchill ◽  
M. Churchill ◽  
A. Bidani ◽  
J. Dunbar
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Model ◽  
Kidney Transplant ◽  
Left Kidney ◽  
Hemodynamic Changes ◽  
Lewis Rats ◽  
Diabetic State ◽  
Renal Hemodynamic ◽  
Renal Clearances ◽  
Per Se

Streptozotocin (STZ)-induced diabetes mellitus is associated with decreased renal clearances of inulin and p-aminohippurate (PAH). The present experiments were designed to determine whether STZ-induced renal hemodynamic changes are due to the drug per se, rather than to the diabetic state that it induces. Isogenic Lewis rats with native right and transplanted left kidneys were studied. In one group, kidney donors received 50 mg STZ/kg body wt on day 1 and transplantation was performed on day 4 (untreated recipients). On day 29, the inulin and PAH clearances of these nondiabetic recipients were, respectively, 0.94 +/- 0.04 and 2.58 +/- 0.11 ml.min-1 x g-1 for the transplanted left kidney (previously exposed to STZ) and 0.95 +/- 0.07 and 2.54 +/- 0.14 ml.min-1 x g-1 for the native right kidney (never exposed to STZ). In another group, recipients received STZ on day 1 and transplantation was performed on day 4 (untreated donors). On day 29, the inulin and PAH clearances of these diabetic recipients were, respectively, 0.62 +/- 0.04 and 1.46 +/- 0.11 ml.min-1 x g-1 for the transplanted left kidney (never exposed to STZ) and 0.61 +/- 0.05 and 1.42 +/- 0.08 ml.min-1 x g-1 for the native right kidney (previously exposed to STZ). We conclude that the diabetic state, rather than STZ, is responsible for the decreased renal clearances of inulin and PAH in this experimental model.

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