scholarly journals Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Yoshinori Aso ◽  
Divya Sitaraman ◽  
Toshiharu Ichinose ◽  
Karla R Kaun ◽  
Katrin Vogt ◽  
...  
Keyword(s):  
Information Flow ◽  
Mushroom Body ◽  
Action Selection ◽  
Cell Type ◽  
Sleep Regulation ◽  
Sensory Stimuli ◽  
Learning Tasks ◽  
Local Stimulus ◽  
Output Neurons

Animals discriminate stimuli, learn their predictive value and use this knowledge to modify their behavior. In Drosophila, the mushroom body (MB) plays a key role in these processes. Sensory stimuli are sparsely represented by ∼2000 Kenyon cells, which converge onto 34 output neurons (MBONs) of 21 types. We studied the role of MBONs in several associative learning tasks and in sleep regulation, revealing the extent to which information flow is segregated into distinct channels and suggesting possible roles for the multi-layered MBON network. We also show that optogenetic activation of MBONs can, depending on cell type, induce repulsion or attraction in flies. The behavioral effects of MBON perturbation are combinatorial, suggesting that the MBON ensemble collectively represents valence. We propose that local, stimulus-specific dopaminergic modulation selectively alters the balance within the MBON network for those stimuli. Our results suggest that valence encoded by the MBON ensemble biases memory-based action selection.

scholarly journals Author response: Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila

2014 ◽  
Author(s):  
Yoshinori Aso ◽  
Divya Sitaraman ◽  
Toshiharu Ichinose ◽  
Karla R Kaun ◽  
Katrin Vogt ◽  
...  
Keyword(s):  
Mushroom Body ◽  
Action Selection ◽  
Author Response ◽  
Output Neurons

scholarly journals Neurogenetic dissection of the Drosophila innate olfactory processing center

2018 ◽  
Author(s):  
Michael-John Dolan ◽  
Shahar Frechter ◽  
Alexander Shakeel Bates ◽  
Chuntao Dan ◽  
Paavo Huoviala ◽  
...  
Keyword(s):  
Small Groups ◽  
Mushroom Body ◽  
Light And Electron Microscopy ◽  
Cell Types ◽  
Structure And Function ◽  
Cell Type ◽  
Gal4 Driver ◽  
Sensory Stimuli ◽  
Innate Behaviour ◽  
And Function

AbstractAnimals exhibit innate behaviours in response to a variety of sensory stimuli such as olfactory cues. In Drosophila, a higher olfactory centre called the lateral horn (LH) is implicated in innate behaviour. However, our knowledge of the structure and function of the LH is scant, due to the lack of sparse neurogenetic tools for this brain region. Here we generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell-types. We identify the neurotransmitter and axo-dendritic polarity for each cell-type. Using these lines were create an anatomical map of the LH. We found that ∼30% of LH projections converge with outputs from the mushroom body, the site of olfactory learning and memory. Finally, using optogenetic activation of small groups of LH neurons. We identify cell-types that drive changes in either valence or specific motor programs, such as turning and locomotion. In summary we have generated a resource for manipulating and mapping LH neurons in both light and electron microscopy and generated insights into the anatomy and function of the LH.

scholarly journals Dopamine neurons promotes wakefulness via the DopR receptor in the Drosophila mushroom body

2020 ◽  
Author(s):  
Margaret Driscoll ◽  
Steven Buchert ◽  
Victoria Coleman ◽  
Morgan McLaughlin ◽  
Amanda Nguyen ◽  
...  
Keyword(s):  
Mushroom Body ◽  
Critical Role ◽  
Fruit Fly ◽  
Dopamine Neurons ◽  
Central Complex ◽  
Behavioral Arousal ◽  
Sleep Homeostasis ◽  
Output Neurons ◽  
Gaba Transmission

AbstractNeural circuits involved in regulation of sleep play a critical role in sleep-wake transition and ability of an organism to engage in other behaviors critical for survival. The fruit fly, Drosophila melanogaster is a powerful system for the study of sleep and circuit mechanisms underlying sleep and co-regulation of sleep with other behaviors. In Drosophila, two neuropils in the central brain, mushroom body (MB) and central complex (CX) have been shown to influence sleep homeostasis and receive neuromodulator input critical to sleep-wake switch.Dopamine neurons (DANs) are the primary neuromodulator inputs to the MB but the mechanisms by which they regulate sleep- and wake-promoting neurons within MB are unknown. Here we investigate the role of subsets of DANs that signal wakefulness and project to wake-promoting compartments of the MB. We find that inhibition of specific subsets of PAM and PPL1 DANs projecting to the MB increase sleep in the presence of strong wake-inducing stimuli that reduces GABA transmission, although activity of these neurons is not directly modulated by GABA signaling. Of these subsets we find that DANs innervating the γ5 and β’2 MB compartments require both DopR1 and DopR2 receptors located in downstream Kenyon cells and mushroom body output neurons (MBONs). Further, we report that unlike the activity of wake-promoting MBONs and KCs, whose activity is modulated by sleep-need and PAM-DAN activity is independent of sleep-need. We have characterized a dopamine mediated sleep-circuit providing an inroad into understanding how common circuits within MB regulate sleep, wakefulness and behavioral arousal.

scholarly journals Neural Substrates of Incidental Associations and Mediated Learning: The Role of Cannabinoid Receptors

2021 ◽  
Vol 15 ◽  
Author(s):  
Christina Ioannidou ◽  
Arnau Busquets-Garcia ◽  
Guillaume Ferreira ◽  
Giovanni Marsicano
Keyword(s):  
Cannabinoid Receptors ◽  
Incidental Learning ◽  
Central Element ◽  
Endocannabinoid System ◽  
Neural Substrates ◽  
Mediated Learning ◽  
Sensory Stimuli ◽  
Learning Tasks ◽  
Everyday Learning

The ability to form associations between different stimuli in the environment to guide adaptive behavior is a central element of learning processes, from perceptual learning in humans to Pavlovian conditioning in animals. Like so, classical conditioning paradigms that test direct associations between low salience sensory stimuli and high salience motivational reinforcers are extremely informative. However, a large part of everyday learning cannot be solely explained by direct conditioning mechanisms – this includes to a great extent associations between individual sensory stimuli, carrying low or null immediate motivational value. This type of associative learning is often described as incidental learning and can be captured in animal models through sensory preconditioning procedures. Here we summarize the evolution of research on incidental and mediated learning, overview the brain systems involved and describe evidence for the role of cannabinoid receptors in such higher-order learning tasks. This evidence favors a number of contemporary hypotheses concerning the participation of the endocannabinoid system in psychosis and psychotic experiences and provides a conceptual framework for understanding how the use of cannabinoid drugs can lead to altered perceptive states.

Auditing the Systems Understanding Aid Project: An Integrative Assignment for Accounting Students

2012 ◽  
Vol 7 (1) ◽  
pp. 27-51 ◽  
Author(s):  
Susan L. Swanger ◽  
Beth H. Jones
Keyword(s):  
Active Learning ◽  
Teaching Methods ◽  
Work Environments ◽  
Accounting Students ◽  
External Work ◽  
Learning Tasks ◽  
Independent Auditors ◽  
Course Integration

ABSTRACT Accounting educators strive to prepare graduates for work in their chosen field. Various teaching methods can be employed to best accomplish this goal. One valuable tool is the use of active learning tasks that simulate external work environments. This paper describes the collaboration between an AIS and an Auditing professor who used an integrative task that spanned their two classes over two semesters. The authors had their AIS students complete Arens and Ward's Systems Understanding Aid (SUA) project (Arens & Ward, 2008). The following semester, students in the auditing class audited the records and financials that had been generated by students the previous semester. The project was designed to facilitate course integration and teamwork by having groups of students play the role of corporate accountants, then act as independent auditors.

Microbiological Detoxification of Hazardous Organic Contaminants: The Crucial Role of Substrate Interactions

1992 ◽  
Vol 25 (11) ◽  
pp. 403-410 ◽  
Author(s):  
B. E. Rittmann
Keyword(s):  
Organic Pollutants ◽  
Information Flow ◽  
Organic Contaminants ◽  
Crucial Role ◽  
Substrate Utilization ◽  
Energy Flows ◽  
Hazardous Pollutants ◽  
Primary Substrate ◽  
Substrate Types

Microbiological detoxification of hazardous organic pollutants is highly promising, but its reliable implementation requires a sophisticated understanding of several different substrate types and how they interact. This paper carefully defines the substrate types and explains how their interactions affect the bacteria's electron and energy flows, information flow, and degradative activity. For example, primary substrates, which are essential for growth and maintenance of the bacteria, also interact with degradation of specific hazardous pollutants by being inducers, inhibitors, and direct or indirect cosubstrates. The target contaminants, which often are secondary substrates, also have the interactive roles of self-inhibitor, inhibitor of primary-substrate utilization, inducer, and a part of an aggregate primary substrate.

Vitamin D and Sleep Regulation: Is there a Role for Vitamin D?

2020 ◽  
Vol 26 (21) ◽  
pp. 2492-2496 ◽  
Author(s):  
Fiammetta Romano ◽  
Giovanna Muscogiuri ◽  
Elea Di Benedetto ◽  
Volha V. Zhukouskaya ◽  
Luigi Barrea ◽  
...  
Keyword(s):  
Vitamin D ◽  
Sleep Disorders ◽  
Vitamin D Deficiency ◽  
Sleep Apnea Syndrome ◽  
Regulatory Mechanisms ◽  
Sleep Regulation ◽  
Vitamin D Receptors ◽  
Obstructive Sleep ◽  
The Impact

Background: Vitamin D exerts multiple pleiotropic effects beyond its role in calcium-phosphate metabolism. Growing evidence suggests an association between hypovitaminosis D and sleep disorders, thus increasing the interest in the role of this vitamin in the regulatory mechanisms of the sleep-wake cycle. Objective: The study aimed to explore and summarize the current knowledge about the role of vitamin D in sleep regulation and the impact of vitamin D deficiency on sleep disorders. Methods: The main regulatory mechanisms of vitamin D on sleep are explained in this study. The literature was scanned to identify clinical trials and correlation studies showing an association between vitamin D deficiency and sleep disorders. Results: Vitamin D receptors and the enzymes that control their activation and degradation are expressed in several areas of the brain involved in sleep regulation. Vitamin D is also involved in the pathways of production of Melatonin, the hormone involved in the regulation of human circadian rhythms and sleep. Furthermore, vitamin D can affect sleep indirectly through non-specific pain disorders, correlated with alterations in sleep quality, such as restless legs syndrome and obstructive sleep apnea syndrome. Conclusions: : Vitamin D has both a direct and an indirect role in the regulation of sleep. Although vitamin D deficiency has been associated to sleep disorders, there is still scant evidence to concretely support the role of vitamin D supplementation in the prevention or treatment of sleep disturbances; indeed, more intervention studies are needed to better clarify these aspects.

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