scholarly journals Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4450 ◽  
Author(s):  
Fanhong Kong ◽  
Wenli Zhang ◽  
Lin Qiao ◽  
Qi Li ◽  
Haowen Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quality Evaluation ◽  
Housekeeping Gene ◽  
Storage Conditions ◽  
Dna Integrity ◽  
Morphological Examination ◽  
Rna Integrity ◽  
Standard Operating

Background We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination. Results The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation. Conclusion Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.

scholarly journals Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital

2017 ◽  
Author(s):  
Fanhong Kong ◽  
Wenli Zhang ◽  
Lin Qiao ◽  
Qi Li ◽  
Haowen Li ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Protein Quality ◽  
Housekeeping Gene ◽  
Storage Conditions ◽  
Dna Integrity ◽  
Morphological Examination ◽  
Rna Integrity ◽  
Standard Operating

Background: We established a glioma biobank at Beijing Tiantan Hospital in November, 2010.Specialized staffs have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods: One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination, housekeeping gene expression and protein integrity determination. Results: The majority of samples (95%) remain high RNA quality for further analysis with RIN≥6. All samples remain high DNA and protein quality without significant degradation. Conclusion: Storage conditions of our biobank are suitable for long-term (at least 5 years) sample preservation with high molecular quality.

scholarly journals Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital

2017 ◽  
Author(s):  
Fanhong Kong ◽  
Wenli Zhang ◽  
Lin Qiao ◽  
Qi Li ◽  
Haowen Li ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Protein Quality ◽  
Housekeeping Gene ◽  
Storage Conditions ◽  
Dna Integrity ◽  
Morphological Examination ◽  
Rna Integrity ◽  
Standard Operating

Background: We established a glioma biobank at Beijing Tiantan Hospital in November, 2010.Specialized staffs have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods: One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination, housekeeping gene expression and protein integrity determination. Results: The majority of samples (95%) remain high RNA quality for further analysis with RIN≥6. All samples remain high DNA and protein quality without significant degradation. Conclusion: Storage conditions of our biobank are suitable for long-term (at least 5 years) sample preservation with high molecular quality.

scholarly journals Facile Use of ZnO Nanopowders to Protect Old Manual Paper Documents

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5452
Author(s):  
Ludmila Motelica ◽  
Aurelian Popescu ◽  
Anca-Gabriela Răzvan ◽  
Ovidiu Oprea ◽  
Roxana-Doina Truşcă ◽  
...  
Keyword(s):  
Hand Hygiene ◽  
Antimicrobial Agents ◽  
Storage Conditions ◽  
Human Pathogens ◽  
Accessible Method

One of the main problems faced by libraries, archives and collectors is the mold degradation of the paper-based documents, books, artworks etc. Microfungi (molds) emerge in regular storage conditions of such items (humidity, usually over 50%, and temperatures under 21 °C). If the removal of the visible mycelium is relatively easy, there is always the problem of the subsequent appearance of mold as the spores remain trapped in the cellulosic, fibrillary texture, which acts as a net. Moreover, due to improper hand hygiene bacteria contamination, old books could represent a source of biohazard, being colonized with human pathogens. An easy and accessible method of decontamination, which could offer long term protection is therefore needed. Here, we present a facile use of the ZnO nanopowders as antimicrobial agents, suitable for cellulose-based products, conferring an extended antibacterial and anti-microfungal effect. The proposed method does not adversely impact on the quality of the cellulose documents and could be efficiently used for biodegradation protection.

Changes in the content of organic acids and inorganic anions in sugar beet during long-term storage

2016 ◽  
pp. 760-764 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Paulina Miko ◽  
Magdalena Molska ◽  
Ilona Baszczyk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Storage Conditions ◽  
Denitrifying Bacteria ◽  
Raw Material ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Acetic Acids

Due to the prolongation of the period of the sugar campaign, it is necessary to optimize the storage conditions, so that changes in the quality of the raw material could be minimized. The aim of this study was to determine the effect of storage duration and temperature on changes in the composition of sugar beet. The study presents the changes in the content of glucose, fructose, raffinose, lactic and acetic acids, nitrates and nitrites as well as in the content of the total number of mesophilic bacteria, denitrifying bacteria and spores of denitrifying bacteria during storage under various conditions.

Evaluation of a low-cost procedure for sampling, long-term storage, and extraction of RNA from blood for qPCR analyses

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Rasmus B. Mærkedahl ◽  
Hanne Frøkiær ◽  
Lotte Lauritzen ◽  
Stine B. Metzdorff
Keyword(s):  
Gene Expression ◽  
Clinical Trials ◽  
Low Cost ◽  
Rna Extraction ◽  
Initial Step ◽  
Rna Integrity ◽  
Long Term Storage ◽  
Significant Cost Reduction ◽  
Term Storage

AbstractIn large clinical trials, where RNA cannot be extracted immediately after sampling, preserving RNA in whole blood is a crucial initial step in obtaining robust qPCR data. The current golden standard for RNA preservation is costly and designed for time-consuming column-based RNA-extraction. We investigated the use of lysis buffer for long-term storage of blood samples for qPCR analysis.Blood was collected from 13 healthy adults and diluted in MagMAX lysis/binding solution or PAXgene Blood RNA tubes and stored at –20 °C for 0, 1, or 4 months before RNA extraction by the matching method. RNA integrity, yield and purity were evaluated and the methods were compared by subsequent analyses of the gene expression levels ofThe MagMAX system extracted 2.3–2.8 times more RNA per mL blood, with better performance in terms of purity, and with comparable levels of integrity relative to the PAXgene system. Gene expression analysis using qPCR of: The MagMAX system can be used for storage of human blood for up to 4 months and is equivalent to the PAXgene system for RNA extraction. It furthermore, provides a means for significant cost reduction in large clinical trials.

scholarly journals Assessment of Shelf-Life Ability of Apples cv. ‘Auksis’ after Long-term Storage Under Different Conditions

2016 ◽  
Vol 24 (2) ◽  
pp. 37-47 ◽  
Author(s):  
Karina Juhņeviča-Radenkova ◽  
Vitalijs Radenkovs
Keyword(s):  
Shelf Life ◽  
Cold Storage ◽  
Storage Conditions ◽  
Soluble Solids ◽  
Market Condition ◽  
Low Oxygen ◽  
Weight Losses ◽  
Long Term Storage

Abstract The objective of the current research was to ascertain the shelf-life ability of apple ‘Auksis’ after 6 months of cold storage under different conditions. The effect of storage conditions such as: cold storage under normal atmosphere (NA), 1-methylcyclopropene (1-MCP) + cold storage, and ultra-low oxygen (ULO)-controlled atmosphere (CA) [2.0% CO2 and 1.0% O2 (ULO1) and 2.5% CO2 and 1.5% O2 (ULO2)] on the quality of apples during shelf-life was evaluated. Apple fruits immediately after cold storage and after 25 days of maintaining at market condition had been evaluated. The physical (firmness, weight losses), chemical (total soluble solids and acid contents), and sensory (aroma, taste, acidity, sweetness, juiciness, and color) characteristics of apples had been evaluated after 5, 10, 15, 20, and 25 days to ascertain maximal shelf-life. Results from sensory evaluation indicated that apples treated with 1-MCP and stored at NA were characterized with distinctive aroma, whereas apples stored under CA were poor in sweetness and had remarkable acidity and juiciness. Apples that were stored in cold had pronounced aroma and color but without taste. Based on the evaluation by panelist, maximum shelf-life of apples that were kept under cold storage and ULO1 was 15 days, whereas that of apples that had been treated with 1-MCP and stored at NA and those stored in ULO2 was 25 days.

scholarly journals Impact of RNA integrity and blood sample storage conditions on the gene expression analysis

2018 ◽  
Vol Volume 11 ◽  
pp. 3573-3581 ◽  
Author(s):  
Yanting Shen ◽  
Rui Li ◽  
Fei Tian ◽  
Zhenzhu Chen ◽  
Na Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Sample ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Storage Conditions ◽  
Sample Storage ◽  
Rna Integrity

scholarly journals Physiological, Biochemical, and Molecular Changes in Pelargonium Cuttings Subjected to Short-term Storage Conditions

1996 ◽  
Vol 121 (6) ◽  
pp. 1063-1068 ◽  
Author(s):  
Richard N. Arteca ◽  
Jeannette M. Arteca ◽  
Tzann-Wei Wang ◽  
Carl D. Schlagnhaufer
Keyword(s):  
Gene Expression ◽  
Acc Oxidase ◽  
Storage Conditions ◽  
Significant Decline ◽  
Short Term ◽  
Molecular Changes ◽  
Visual Rating ◽  
Shoot Weight ◽  
Term Storage

The purpose of this study was to evaluate physiological, biochemical, and molecular changes that occur in unrooted Pelargonium ×hortorum cuttings during storage. Pelargonium cuttings of `Sincerity' (good shipper), `Wendy Ann' (moderate shipper) and `Snowmass' (poor shipper) were stored at 25 °C and evaluated over a 5-day period. Following removal from storage, cuttings of all cultivars exhibited steady and significant decline in photosynthesis, respiration, carbohydrate, starch, and protein over time. However, no significant differences were observed among cultivars for all of these parameters. Ethylene levels produced by `Sincerity' and `Wendy Ann' began to increase 3 days following storage; whereas, `Snowmass' showed an increase after 1 day, reaching a peak at 3 days, and then declined. When unrooted cuttings of `Snowmass' were stored for 5 days at temperatures ranging from 4 to 25 °C, it was observed that those stored at 4 °C had a significantly higher visual rating, chlorophyll content, and root and shoot weight than at higher temperatures tested. As temperature increased from 10 to 25 °C, quality of cuttings declined. Changes in gene expression of two ACC synthases and an ACC oxidase were evaluated in `Snowmass' cuttings stored at 4 and 25 °C. Correlations between ethylene and ACC levels with gene expression were observed. Chemical name used: 1-aminocyclopropane-1-carboxylic acid (ACC).

scholarly journals Storage Temperature, Controlled Atmosphere, and 1-Methylcyclopropene Effects on α-Farnesene, Conjugated Trienols, and Peroxidation in Relation with Superficial Scald, Pithy Brown Core, and Fruit Quality of ‘d’Anjou’ Pears during Long-term Storage

2016 ◽  
Vol 141 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Yan Wang
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Metabolic Balance ◽  
Superficial Scald ◽  
Long Term Storage ◽  
Temperature Controlled ◽  
Term Storage

Alternatives to ethoxyquin (Etq) are needed for controlling superficial scald of ‘Anjou’ european pears (Pyrus communis) during long-term storage. The current commercial standard storage conditions [Etq + −1 °C + controlled atmosphere (CA) with 1.5 kPa O2] reduced scald occurrence compared with control fruit (−1 °C + CA) during 6–8 months storage. At 1 °C in air, 1-methylcyclopropene (1-MCP) fumigation at 0.15 µL·L−1 at harvest was more efficient on reducing scald than Etq but did not prevent scald during 6–8 months storage. The 1-MCP-treated fruit at 1 °C in air developed their ripening capacity at 20 °C following 6–8 months storage but had deceased shipping ability (softening and yellowing of fruit). Although Etq inhibition of scald was associated with the inhibition of α-farnesene oxidation to conjugated trienols (CTols); 1-MCP reduced α-farnesene synthesis and thereby the availability of substrate to oxidize to CTols. CA storage at 1.5 kPa O2 totally prevented scald and retarded the loss of shipping ability without affecting the ripening capacity of 1-MCP-treated fruit at 1 °C through further decreases in the syntheses of ethylene, α-farnesene and CTols during 6–8 months storage. In addition, 1-MCP prevented a CA-induced disorder, pithy brown core (PBC), in ‘Anjou’ pears possibly through enhancing an oxidative/reductive metabolic balance during extended storage. In conclusion, the combinations of 1 °C + 1-MCP + CA is a potential commercial alternative to Etq for scald control while allowing the 1-MCP-treated ‘Anjou’ pears to recover ripening capacity during the shelf life period after 6–8 months storage.

scholarly journals Quality assessment of RNA in long-term storage: The All Our Families biorepository

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0242404
Author(s):  
Nikki L. Stephenson ◽  
Kylie K. Hornaday ◽  
Chelsea T. A. Doktorchik ◽  
Andrew W. Lyon ◽  
Suzanne C. Tough ◽  
...  
Keyword(s):  
Population Based ◽  
Rate Of Change ◽  
Population Based Study ◽  
Acid Yield ◽  
Rna Integrity ◽  
Long Term Storage ◽  
Term Storage ◽  
Over Time

Background The All Our Families (AOF) cohort study is a longitudinal population-based study which collected biological samples from 1948 pregnant women between May 2008 and December 2010. As the quality of samples can decline over time, the objective of the current study was to assess the association between storage time and RNA (ribonucleic acid) yield and purity, and confirm the quality of these samples after 7–10 years in long-term storage. Methods Maternal whole blood samples were previously collected by trained phlebotomists and stored in four separate PAXgene Blood RNA Tubes (PreAnalytiX) between 2008 and 2011. RNA was isolated in 2011 and 2018 using PAXgene Blood RNA Kits (PreAnalytiX) as per the manufacturer’s instruction. RNA purity (260/280), as well as RNA yield, were measured using a Nanodrop. The RNA integrity number (RIN) was also assessed from 5–25 and 111–130 months of storage using RNA 6000 Nano Kit and Agilent 2100 BioAnalyzer. Descriptive statistics, paired t-test, and response feature analysis using linear regression were used to assess the association between various predictor variables and quality of the RNA isolated. Results Overall, RNA purity and yield of the samples did not decline over time. RNA purity of samples isolated in 2011 (2.08, 95% CI: 2.08–2.09) were statistically lower (p<0.000) than samples isolated in 2018 (2.101, 95% CI: 2.097, 2.104), and there was no statistical difference between the 2011 (13.08 μg /tube, 95% CI: 12.27–13.89) and 2018 (12.64 μg /tube, 95% CI: 11.83–13.46) RNA yield (p = 0.2964). For every month of storage, the change in RNA purity is -0.01(260/280), and the change in RNA yield between 2011 and 2018 is -0.90 μ g / tube. The mean RIN was 8.49 (95% CI:8.44–8.54), and it ranged from 7.2 to 9.5. The rate of change in expected RIN per month of storage is 0.003 (95% CI 0.002–0.004), so while statistically significant, these results are not relevant. Conclusions RNA quality does not decrease over time, and the methods used to collect and store samples, within a population-based study are robust to inherent operational factors which may degrade sample quality over time.

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