scholarly journals Degradability of Plastics in Mangrove Soil from Eco-Mangrove Reserve in Calapan City, Oriental Mindoro Philippines

2018 ◽  
Vol 31 (1) ◽  
Author(s):  
Jermaine Ritzchelle D. Marquez
Keyword(s):  
Nutrient Medium ◽  
Mineral Salt Medium ◽  
Bacterial Species ◽  
Rich Source ◽  
Mineral Salt ◽  
Salt Medium ◽  
Weight Changes ◽  
Local Study ◽  
Mangrove Soil ◽  
Degrading Bacteria

Mangrove forests are indigenous to tropical as well as subtropical regions worldwide. Mangrove soil is a rich source of plastic-degrading bacteria but no local study has been done to support its potential benefits.  This study was conducted to assess the suitability of an improvised nutrient medium for the isolation of plastic-degrading bacteria from mangrove soil obtained from Silonay Eco-Mangrove Reserve, Calapan City, Philippines. Forty-Two (42) Winogradsky column WC), the first 21 WC contain mineral salt medium and the remaining 21 contain sea salt medium. Plastic samples were observed for 36 days by comparing the initial and final weights. WC with mineral salt medium showed the most changes in the final plastic weight while the seawater medium Winogradsky column showed less plastic weight changes. Laboratory test showed that plastic degrading bacteria that were gram-negative were Citrobacter fruendii, Enterobacter agglomerans, Pseudomonas aeruginosa and Arizona spp. The study showed that mangrove soil in Calapan City, Oriental Mindoro is a rich source of biodegrading bacteria. The use of improvised nutrient medium solves the unavailability of expensive materials and can be used in exploring the potential use of identified bacteria for future work in biotechnology.    Further studies need to be done to investigate other bacterial species aside from identified bacteria that can biodegrade plastics.

scholarly journals Biodegradation of Crystal Violet dye by bacteria isolated from textile industry effluents

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5015 ◽  
Author(s):  
Dipankar Chandra Roy ◽  
Sudhangshu Kumar Biswas ◽  
Ananda Kumar Saha ◽  
Biswanath Sikdar ◽  
Mizanur Rahman ◽  
...  
Keyword(s):  
Crystal Violet ◽  
Textile Industry ◽  
Mineral Salt Medium ◽  
Industrial Effluent ◽  
Textile Dyes ◽  
Mineral Salt ◽  
Effluent Treatment ◽  
Salt Medium ◽  
Degrading Bacteria ◽  
Crystal Violet Dye

Industrial effluent containing textile dyes is regarded as a major environmental concern in the present world. Crystal Violet is one of the vital textile dyes of the triphenylmethane group; it is widely used in textile industry and known for its mutagenic and mitotic poisoning nature. Bioremediation, especially through bacteria, is becoming an emerging and important sector in effluent treatment. This study aimed to isolate and identify Crystal Violet degrading bacteria from industrial effluents with potential use in bioremediation. The decolorizing activity of the bacteria was measured using a photo electric colorimeter after aerobic incubation in different time intervals of the isolates. Environmental parameters such as pH, temperature, initial dye concentration and inoculum size were optimized using mineral salt medium containing different concentration of Crystal Violet dye. Complete decolorizing efficiency was observed in a mineral salt medium containing up to 150 mg/l of Crystal Violet dye by 10% (v/v) inoculums of Enterobacter sp. CV–S1 tested under 72 h of shaking incubation at temperature 35 °C and pH 6.5. Newly identified bacteria Enterobacter sp. CV–S1, confirmed by 16S ribosomal RNA sequencing, was found as a potential bioremediation biocatalyst in the aerobic degradation/de-colorization of Crystal Violet dye. The efficiency of degrading triphenylmethane dye by this isolate, minus the supply of extra carbon or nitrogen sources in the media, highlights the significance of larger-scale treatment of textile effluent.

Metabolism of a monoterpene alcohol, linalool, by a soil pseudomonad

1977 ◽  
Vol 23 (3) ◽  
pp. 230-239 ◽  
Author(s):  
K. M. Madyastha ◽  
P. K. Bhattacharyya ◽  
C. S. Vaidyanathan
Keyword(s):  
Mineral Salt Medium ◽  
Enrichment Culture ◽  
Sole Source ◽  
Mineral Salt ◽  
Salt Medium ◽  
Culture Techniques ◽  
Unsaturated Lactone ◽  
Linalool Oxide

A microorganism of the genus Pseudomonas has been isolated from the soil by enrichment culture techniques with linalool(I) as the sole source of carbon and energy. The organism is also capable of utilizing limonene, citronellol, and geraniol as substrates but fails to grow on citral, citranellal, and 1,8-cineole. Fermentation of linalool by this bacterium in a mineral salt medium results in the formation of 10-hydroxylinalool(II), 10-carboxylinalool(III), oleuropeic acid(IX), 2-vinyl-2-methyl-5-hydroxyisopropyl-tetrahydrofuran(linalool oxide, V), 2-vinyl-2-methyl-tetrahydrofuran-5-one(unsaturated lactone, VI), and few unidentified minor metabolites. Probable pathways for the biodegradation of linalool are presented.

scholarly journals Removal of tannin from Shea nut cake by Pseudomonas strain

1970 ◽  
Vol 5 (1) ◽  
pp. 17-22
Author(s):  
F.M. Danikuu ◽  
A. Quainoo ◽  
E.N.K. Sowley
Keyword(s):  
Mineral Salt Medium ◽  
Cost Effective ◽  
Sole Source ◽  
Northern Region ◽  
Mineral Salt ◽  
Salt Medium ◽  
Biomedical Sciences ◽  
Nutritional Factors ◽  
Tannin Concentration ◽  
Enhanced Biodegradation

The use of microorganisms to remove the anti-nutritional factors in shea nut cake as the most cost effective and environmentally friendly approach to permit its use in agriculture for animal nutrition and soil fertility is long anticipated. Bacteria isolated from shea nut cake polluted soil in Sagnarigu District of Northern Region of Ghana showed that anti-nutritional factors in shea nut cake are bio-degradable. The bacteria were grown in mineral salt medium supplemented with 2% shea nut cake as sole source of carbon. The bacteria isolate was identified biochemically as Pseudomonas aerugenosa and reduced total tannin concentration in shea nut cake from 54.58 g Kg-1 to 8.71 g Kg-1 (84%) in 10 days and 92% in 20 days. Boiling of shea nut cake reduced tannin content from 54.58 g Kg-1 to 16.36 g Kg-1 (70%) and enhanced biodegradation of tannin in the shea nut cake, removing up to 95% of total tannins in shea nut cake in 20 days.Journal of Medical and Biomedical Sciences (2016) 5(1), 17-22Keywords: Tannins, biodegradability, bacteria, mineral salt medium

scholarly journals Enrichment and Analysis of Stable 1,4-dioxane-Degrading Microbial Consortia Consisting of Novel Dioxane-Degraders

2019 ◽  
Vol 8 (1) ◽  
pp. 50 ◽  
Author(s):  
Tanmoy Roy Tusher ◽  
Takuya Shimizu ◽  
Chihiro Inoue ◽  
Mei-Fang Chien
Keyword(s):  
Ribosomal Rna ◽  
Industrial Wastewater ◽  
Microbial Consortium ◽  
Mineral Salt Medium ◽  
Intergenic Spacer ◽  
Mineral Salt ◽  
Microbial Consortia ◽  
Salt Medium ◽  
Bacterial Strains ◽  
Water Contaminant

Biodegradation of 1,4-dioxane, a water contaminant of emerging concern, has drawn substantial attention over the last two decades. A number of dioxane-degraders have been identified, though many of them are unable to metabolically utilize 1,4-dioxane. Moreover, it is considered more preferable to use microbial consortia rather than the pure strains, especially in conventional bioreactors for industrial wastewater treatment. In the present study, a stable 1,4-dioxane-degrading microbial consortium was enriched, namely 112, from industrial wastewater by nitrate mineral salt medium (NMSM). The consortium 112 is capable of utilizing 1,4-dioxane as a sole carbon and energy source, and can completely degrade 1,4-dioxane up to 100 mg/L. From the consortium 112, two 1,4-dioxane-degrading bacterial strains were isolated and identified, in which the Variovorax sp. TS13 was found to be a novel 1,4-dioxane-degrader that can utilize 100 mg/L of 1,4-dioxane. The efficacy of the consortium 112 was increased significantly when we cultured the consortium with mineral salt medium (MSM). The new consortium, N112, could utilize 1,4-dioxane at a rate of 1.67 mg/L·h. The results of the ribosomal RNA intergenic spacer analysis (RISA) depicted that changes in the microbial community structure of consortium 112 was the reason behind the improved degradation efficiency of consortium N112, which was exhibited as a stable and effective microbial consortium with a high potential for bioremediation of the dioxane-impacted sites and contaminated industrial wastewater.

scholarly journals PRODUCTION OF LIPASE BY IMMOBILIZED BACILLUS THURINGIENSIS AND LYSINIBACILLUS SPHAERICUS AND THEIR BIODEGRADATION POTENTIAL ON DIESEL

2020 ◽  
Vol 3 (3) ◽  
pp. 52-57
Author(s):  
Olaposi Adegoke ◽  
Sarafadeen Kareem ◽  
Saka Balogun ◽  
Adeniyi Afolabi
Keyword(s):  
Bacillus Thuringiensis ◽  
16S Rrna ◽  
Mineral Salt Medium ◽  
Lipase Production ◽  
Mineral Salt ◽  
Oil Degradation ◽  
Salt Medium ◽  
Gelatin Matrix ◽  
Lysinibacillus Sphaericus ◽  
Ms Analysis

This study reported production of lipase by immobilized Bacillus thuringiensis. Bacteria isolates were screened on Bushnell-Hass Mineral Salt medium containing 1% v/v Diesel for oil degradation. The potent isolates were identified using 16S rRNA as Bacillus thuringiensis. The isolates were immobilized in gelatin matrix and cultured for lipase production in a submerged medium. The crude lipase extracted was used for degradation of Diesel. Optimum degradation of Diesel 41.4% was obtained by lipase from Immobilized Bacillus thuringiensis and 31.6% for Lysinibacillus sphaericus at pH 7 and 35 in 20 days. GC-MS analysis was carried out to show the compounds degraded after 20 days. This study therefore presented the use of immobilized bacterial lipase in degradation of Diesel as a simple and effective approach.

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