A Comparison of Four Methods (Immunochemistry and HPLC) for Determination of 25-(OH)-Vitamin D in Postmenopausal Women

Vitamin D deficiency in pregnant women and their offspring may result in unfavorable health outcomes for both mother and infant. A 25hydroxyvitamin D (25(OH)D) level of at least 75 nmol/L is recommended by the Endocrine Society. Validated, automated sample preparation and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were used to determine the vitamin D metabolites status in mother-infant pairs. Detection of 3-Epi25(OH)D3 prevented overestimation of 25(OH)D3 and misclassification of vitamin D status. Sixty-three percent of maternal 25(OH)D plasma levels were less than the recommended level of 25(OH)D at 3 months. Additionally, breastmilk levels of 25(OH)D decreased from 60.1 nmol/L to 50.0 nmol/L between six weeks and three months (p < 0.01). Furthermore, there was a positive correlation between mother and infant plasma levels (p < 0.01, r = 0.56) at 3 months. Accordingly, 31% of the infants were categorized as vitamin D deficient (25(OH)D < 50 nmol/L) compared to 25% if 3-Epi25(OH)D3 was not distinguished from 25(OH)D3. This study highlights the importance of accurate quantification of 25(OH)D. Monitoring vitamin D metabolites in infant, maternal plasma, and breastmilk may be needed to ensure adequate levels in both mother and infant in the first 6 months of infant life.

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Association Of Vitamin D Deficiency And Pelvic Organ Prolapse In Postmenopausal Women: A Cross-Sectional Study

10.21203/rs.3.rs-574096/v1 ◽

2021 ◽

Author(s):

Mateja Legan ◽

Matija Barbič ◽

Josko Osredkar ◽

Mija Blaganje

Keyword(s):

Vitamin D ◽

Pelvic Organ Prolapse ◽

Vitamin D Deficiency ◽

Postmenopausal Women ◽

Test Group ◽

Pelvic Organ ◽

Cross Sectional ◽

Vaginal Deliveries ◽

Vitamin D Levels ◽

25 Oh Vitamin D

Abstract Background: Vitamin D is vital for skeletal integrity as well as optimal muscle work. High incidence and prevalence of vitamin D deficiency as well as pelvic organe prolapse are found in postmenopausal women, thus raising the question of whether the entities could be related.Methods: We compared 50 postmenopausal women with pelvic organ prolapse (POP) with 48 age- and weight-matched controls. The clinical assessment of the disorder was performed using the Pelvic Organ Prolapse Quantification system (POP-Q). A questionnaire was filled out by the participants and a blood sample was collected for 25-OH-vitamin D determination.Results: The two groups did not differ significantly in body mass index, but differed significantly in vitamin D blood level concentrations, those being lower in POP patients. A significantly higher prevalence of vitamin D deficiency (25-OH-vitamin D< 50 nmol/l) was found in the test group compared to controls. Higher parity/vaginal deliveries and less caesarean sections were found in patients than in controls. Conclusions: Vitamin D deficiency might be an important associated systemic factor associated to pelvic organ prolapse. The determination of vitamin D levels in postmenopausal women and replenishing its deficiency might also be of importance for the pelvic floor.

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Vitamin D intake, Outdoor Activity Time and Serum 25-OH Vitamin D Concentrations of Korean Postmenopausal Women by Season and by Age

Korean Journal of Community Nutrition ◽

10.5720/kjcn.2015.20.2.120 ◽

2015 ◽

Vol 20 (2) ◽

pp. 120 ◽

Cited By ~ 4

Author(s):

Jin-Sook Yoon ◽

Min-Kyoung Song

Keyword(s):

Vitamin D ◽

Postmenopausal Women ◽

Outdoor Activity ◽

Activity Time ◽

Vitamin D Intake ◽

25 Oh Vitamin D

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Simultaneous determination of vitamins A, E and 25-OH-vitamin D: Application in clinical assessments

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2005.11.004 ◽

2006 ◽

Vol 39 (2) ◽

pp. 180-182 ◽

Cited By ~ 21

Author(s):

F. Granado-Lorencio ◽

B. Olmedilla-Alonso ◽

C. Herrero-Barbudo ◽

I. Blanco-Navarro ◽

S. Blázquez-García ◽

...

Keyword(s):

Vitamin D ◽

Simultaneous Determination ◽

Clinical Assessments ◽

25 Oh Vitamin D

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Weekly alendronate plus vitamin D3 5600 IU vs. standard care: Effect on serum 25(OH) vitamin D, bone turnover markers, and BMD in osteoporotic postmenopausal women with vitamin D inadequacy — 1-year results of a randomized trial

Bone ◽

10.1016/j.bone.2011.03.504 ◽

2011 ◽

Vol 48 ◽

pp. S218

Author(s):

D.P. Kiel ◽

N. Binkley ◽

S. Boonen ◽

S. Ralston ◽

J.-Y. Regnister ◽

...

Keyword(s):

Vitamin D ◽

Bone Turnover ◽

Randomized Trial ◽

Postmenopausal Women ◽

Vitamin D3 ◽

Bone Turnover Markers ◽

Standard Care ◽

Turnover Markers ◽

25 Oh Vitamin D

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Study to determine whether SNPs in CYP17A1, ESR1, and TNRC9 loci correlate with increased breast cancer risk and serum 25-OH vitamin D levels.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e13086 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e13086-e13086 ◽

Cited By ~ 1

Author(s):

Nobuyasu Yoshimoto ◽

Yu Dong ◽

Hiroshi Sugiura ◽

Mitsuo Terada ◽

Naoto Kondo ◽

...

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

High Risk ◽

Breast Cancer Risk ◽

Cancer Risk ◽

Postmenopausal Women ◽

Premenopausal Women ◽

Individual Snps ◽

Significant Difference ◽

25 Oh Vitamin D

e13086 Background: Low serum 25-OH vitamin D (25-OH Vit.D) level is associated with a higher risk of breast cancer. Many single nucleotide polymorphisms (SNP) are associated with breast cancer risk, although the mechanisms underlying this association in most cases remain unclear. We attempted to address this issue by determining whether specific SNPs were correlated with breast cancer-associated biomarkers (BM). Methods: We analyzed estrogen receptor (ER)-positive breast cancer patients treated between Jan 2011 and Dec 2014. We measured serum testosterone and 25-OH Vit.D in pre- and post-menopausal women, and also measured estradiol in the latter group. We genotyped 7 individual SNPs both in premenopausal and postmenopausal women. We then looked for correlations between BMs and SNPs in all three genotypes (homozygotes of major alleles, heterozygotes and homozygotes of minor alleles) using the Kruskal-Wallis test. For each SNP locus associated with a trend or significant effect with regard to BM levels, a t-test was then applied to determine which of the two genotypes was responsible for the effect. Results: Four hundred thirteen women were enrolled (163 premenopausal patients, and 250 postmenopausal patients). There was a trend difference between CYP17A1 rs743572 and 25-OH Vit.D (p = 0.0888), ESR1 rs204210 and 25-OH Vit.D (p = 0.0534) in premenopausal women, and a significant difference between TNRC9 rs3803662 and 25-OH Vit.D (p = 0.026) in postmenopausal women. Among premenopausal women, 25-OH Vit.D levels were significantly lower in those with the high-risk rs743572 genotype TC when compared with TT individuals (19.2 +/- 6.7 ng/ml vs 22.9 +/- 8.7 ng/ml; p = 0.0125). Similarly, 25-OH Vit.D was significantly lower in women with the high-risk rs204210 genotype AG when compared with GG individuals (19.1 +/- 6.9 ng/ml vs 21.7 +/- 6.4 ng/ml; p = 0.0219). In postmenopausal women, 25-OH Vit.D was significantly lower in women with the high-risk rs3803662 genotype GG than those with the AA genotype (20.5 +/- 9.2 ng/ml vs 24.4 +/- 8.0 ng/ml; p = 0.0104). Conclusions: Specific SNPs in CYP17A1, ESR1, and TNRC9 genes may modulate breast cancer risk due to their influence on serum 25-OH Vit.D levels.

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