scholarly journals Estimation of Glycated Haemoglobin by Nephelometry, Ion Exchange Resin and High Performance Liquid Chromatography: A Cross-sectional Study

2020 ◽  
Author(s):  
Mangalore Balakrishna Prathima ◽  
Shridhar Reshma ◽  
. Sushith ◽  
Prathiksha Shetty ◽  
D'Sa Janice ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Gold Standard ◽  
High Performance ◽  
Glycated Haemoglobin ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
The Mean ◽  
R Value

Introduction: Diabetes mellitus cases are continually rising all over the world. Glycated haemoglobin (HbA1c) used as a diagnostic test to measure long-term average glycaemic control in diabetic patients. Aim: To assess the precision and reproducibility of the Ion Exchange Column Chromatography Resin (IECR) method and nephelometry in comparison to High-Performance Liquid Chromatography (HPLC) with respect to the estimation of HbA1c. Materials and Methods: A cross-sectional comparative study was conducted on 50 blood samples, collected from the diabetic subjects at the Department of Biochemistry and their HbA1c values were estimated by HPLC based BioRad D-10, nephelometry and IECR techniques. HPLC was used as a gold standard method, to evaluate the sensitivity and specificity of nephelometry and IECR techniques. Data were expressed as mean±standard deviation and intraclass Correlation Coefficient and Pearson correlation were calculated. Results: The mean age of the study subjects was 53.06±7.67 years. The mean plasma HbA1c levels were 8.16±2.9, 7.62±2.5 and 7.84±2.5 and mean estimated Average Glucose (eAG) was 187.00±84.4, 172.0±76.8, and 289.46±199.9 by HPLC, Nephelometry and IECR, respectively. Compared with HPLC, nephelometry had excellent correlation (r-value 0.925); p<0.001) and IECR (r-value 0.869; p<0.001). Conclusion: Nephelometry and IECR both had better performance and showed a greater concordance with gold standard HPLC. Therefore, nephelometry and IECR can be used as an alternative assay for HbA1c estimation.

Haemoglobin variants may cause significant differences in haemoglobin A1c as measured by high-performance liquid chromatography and enzymatic methods in diabetic patients: a cross-sectional study

2016 ◽  
Vol 54 (4) ◽  
pp. 432-437 ◽  
Author(s):  
Shuichi Otabe ◽  
Hitomi Nakayama ◽  
Tsuyoshi Ohki ◽  
Eri Soejima ◽  
Yuji Tajiri ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Globin Gene ◽  
Cross Sectional Study ◽  
Enzymatic Assay ◽  
Diabetic Patients ◽  
Haemoglobin A1c ◽  
Cross Sectional ◽  
Hb Toranomon

Background We aimed to determine whether the discrepancy between haemoglobin A1c values determined by high-performance liquid chromatography and enzymatic haemoglobin A1c measurements in diabetic patients was clinically relevant. Methods We randomly recruited 1421 outpatients undergoing diabetic treatment and follow-up who underwent at least three haemoglobin A1c measurements between April 2014 and March 2015 at our clinic. In 6369 samples, haemoglobin A1c was simultaneously measured by HA-8160 and MetaboLead (enzymatic assay), and the values were compared. Results haemoglobin A1c measurements by high-performance liquid chromatography and enzymatic assay were strongly correlated (correlation coefficient: 0.9828, linear approximation curve y = 0.9986x − 0.2507). Mean haemoglobin A1c (6.8 ± 1.0%) measured by high-performance liquid chromatography was significantly higher than that measured by enzymatic assay (6.5 ± 1.0%, P < 0.0001). During the sample processing, four (0.3%) subjects presented consistently lower haemoglobin A1c values (<0.7%) by high-performance liquid chromatography than those from enzymatic assay. Of these, three had Hb Toranomon [β112 (G14) Cys→Trp]. The fourth had Hb Ube-2 [α68 (E17) Asn→Asp]. One other subject presented consistently higher haemoglobin A1c values (>1%) by high-performance liquid chromatography than those from enzymatic assay and was diagnosed with a −77 (T > C) mutation in the δ-globin gene. These unrelated asymptomatic subjects had normal erythrocyte profiles, without anaemia. Conclusions We showed that haemoglobin A1c values measured by high-performance liquid chromatography were significantly higher than those measured by enzymatic assay in diabetic subjects. However, when an oversized deviation (>0.7%) between glycaemic control status and haemoglobin A1c is apparent, clinicians should check the methods used to measure haemoglobin A1c and consider the possible presence of a haemoglobin variant.

scholarly journals Comparison of Turbidimetric Inhibition Immunoassay, High-Performance Liquid Chromatography, and Capillary Electrophoresis Methods for Glycated Hemoglobin Determination

2020 ◽  
Vol 51 (6) ◽  
pp. 579-584
Author(s):  
Mehwish Gilani ◽  
Mohammad Aamir ◽  
Ammad Akram ◽  
Zujaja Hina Haroon ◽  
Aamir Ijaz ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Clinical Chemistry ◽  
Method Comparison ◽  
Armed Forces ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
The Mean

Abstract Objective The purpose of this study was to compare the performances of and evaluate the agreement among glycated hemoglobin values analyzed by using National Glycohemoglobin Standardization Program-certified and International Federation of Clinical Chemistry-standardized analyzers. This cross-sectional study was conducted at the Armed Forces Institute of Pathology, Department of Chemical Pathology from March 2019 to May 2019. Methods Glycated hemoglobin (HbA1c) was measured in the blood specimens from 100 patients on an ADVIA 1800 by a turbidimetric inhibitory immunoassay (TINIA), Sebia instrument by electrophoresis, and Bio-Rad Variant II Turbo system by high-performance liquid chromatography (HPLC). Quantitative variables were calculated as the mean ± standard deviation (SD). Precision and method comparisons were carried out according to Clinical and Laboratory Standards Institute recommendations. The results obtained from each analyzer were compared by correlation analysis. Method comparison was done by linear regression and Bland-Altman plots using the SPSS software version 24. Results The mean ± SD HbA1c values from TINIA, electrophoresis, and HPLC were 7.188% ± 1.89%, 7.164% ± 1.866%, and 7.160% ± 1.85%, respectively. The between-run coefficients of variation for TINIA, electrophoresis, and HPLC were 0.64%, 0.61%, and 0.60%, respectively. All 3 showed good correlation (TINIA, R2 = .994, P = .00; electrophoresis, R2 = .992, P = 0.00; and HPLC, R2 = .994, P = 0.00). Conclusion The good clinical agreements of HbA1c and strong correlations between analyzers indicate that these analyzers can be used interchangeably.

scholarly journals Association between Diabetic Retinopathy and Chronic Periodontitis—A Cross-Sectional Study

2018 ◽  
Vol 6 (4) ◽  
pp. 104 ◽  
Author(s):  
Veena H. R. ◽  
Sribhargava Natesh ◽  
Sudhir Patil
Keyword(s):  
Diabetic Retinopathy ◽  
Serum Creatinine ◽  
Glycated Haemoglobin ◽  
Cross Sectional Study ◽  
Diabetic Patients ◽  
Age Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Chronic Inflammatory Condition ◽  
The Mean

Periodontal disease (PD), a chronic inflammatory condition characterized by destruction of the supporting tissues of the teeth, increases the risk of complications in diabetics. Diabetic retinopathy (DR) is a microvascular complication of prolonged hyperglycaemia. There appears to be a similarity in the pathogenesis of DR and PD. Hence, this study aimed to investigate the association, if any, between DR and PD, correlate the severity of DR with the severity of PD, and investigate the association between glycated haemoglobin (HbA1c), serum creatinine and periodontal variables. The periodontal status of 200 adult diabetic patients in the age group of 30–65 years with varying severity of DR was assessed. Evaluation of the severity of PD was assessed by recording clinical parameters. Haematological investigations including glycated haemoglobin (HbA1c) and serum creatinine were estimated before the initiation of treatment for DR. A statistically significant association between the mean duration of diabetes mellitus (DM) and the severity of DR and PD was found. The severity of PD was directly correlated with the severity of DR. There was a significant association between the levels of HbA1c and serum creatinine and severity of DR and PD. There could be a plausible relationship between DR and PD. Further prospective studies on a larger population with longer follow-ups are required to ascertain whether PD and its severity directly affect the progression and severity of DR.

Occurrence of Aflatoxin M1 in Cheeses from the South of Spain

1996 ◽  
Vol 59 (8) ◽  
pp. 898-900 ◽  
Author(s):  
Mª JOSÉ BARRIOS ◽  
Mª JESÚS GUALDA ◽  
J. M. CABANAS ◽  
L. M. MEDINA ◽  
R. JORDANO
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Aflatoxin M1 ◽  
The South ◽  
Different Types ◽  
The Mean

Thirty-five samples of commercial cheeses, 9 fresh, 9 semicured or semiripened and 17 ripened made with different types of milk (cow, ewe, goat and mixtures of milk of various species) produced in the South of Spain were analyzed for the presence of aflatoxin M1 (AFM1) by high-performance liquid chromatography, In 16 of the 35 samples (45.71%) the presence of AFM1 was detected in concentrations ranging between 20 and 200 ng/g of cheese, In the positive cases, the mean levels of AFM1 were 105.33 ng/g in ripened cheeses, 73.80 ng/g in semiripened cheeses and 42.60 ng/g in fresh cheeses.

scholarly journals ANALISIS KADAR BENZOAT DAN SORBAT PADA SAUS SAMBAL KEMASAN YANG DIJUAL DI PASAR BARU BEKASI DENGAN METODE HPLC

2020 ◽  
Vol 3 (1) ◽  
pp. 22-27
Author(s):  
Maulidya Mitha Rianto ◽  
Elfira Mayasari ◽  
Siti Nurfajriah
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cross Sectional ◽  
Hplc Data

Pendahuluan: Bahan Tambahan Pangan (BTP) digunakan secara luas oleh masyarakat. Bahan Tambahan Pangan (BTP) yang banyak digunakan dalam saus sambal adalah pengawet seperti benzoat dan sorbat. Penggunaan pengawet pada bahan pangan tidak boleh melebihi ambang batas yang sudah ditentukan karena dapat memberikan efek bagi kesehatan, seperti benzoat yang dapat menimbulkan reaksi alergi dan bersifat karsinogenik karena adanya benzene serta sorbat yang dapat menyebabkan mutasi gen bila dikombinasikan dengan asam askorbat. Penelitian ini bertujuan untuk mengetahui kadar benzoat dan sorbat pada saus sambal kemasan yang dijual di Pasar Baru Bekasi dan melihat kesesuaian kadar pengawet dengan ambang batas yang sudah ditentukan. Metode: Metode penelitian yang digunakan adalah deskriptif kuantitatif dengan pendekatan cross-sectional. Kadar benzoat dan sorbat diperiksa menggunakan metode High Performance Liquid Chromatography (HPLC). Data kuantitatif yang diperoleh dari hasil pengukuran dianalisis dengan membandingkan kadar maksimum benzoat dan sorbat yang diperbolehkan pada saus menurut Peraturan Kepala BPOM No. 36 Tahun 2013, yaitu 1000 mg/kg. Kadar benzoat dan sorbat yang melebihi ambang batas menandakan bahwa saus sambal tersebut tidak boleh untuk dikonsumsi. Hasil: Hasil penelitian menunjukkan bahwa seluruh 100% sampel mengandung benzoat melebihi kadar maksimum yang telah ditetapkan dan 50% sampel mengandung sorbat dengan kadar di bawah ambang batas yang telah ditentukan. Kesimpulan: Berdasarkan penelitian yang telah dilakukan, saran yang dapat diberikan untuk penelitian selanjutnya adalah melakukan analisis kadar benzoat dan sorbat pada sampel bahan pangan yang lain mengingat bahwa benzoat dan sorbat sering digunakan sebagai kombinasi pengawet dan diperbolehkan untuk digunakan dalam bahan pangan.

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