scholarly journals Comparison of Turbidimetric Inhibition Immunoassay, High-Performance Liquid Chromatography, and Capillary Electrophoresis Methods for Glycated Hemoglobin Determination

2020 ◽  
Vol 51 (6) ◽  
pp. 579-584
Author(s):  
Mehwish Gilani ◽  
Mohammad Aamir ◽  
Ammad Akram ◽  
Zujaja Hina Haroon ◽  
Aamir Ijaz ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glycated Hemoglobin ◽  
Clinical Chemistry ◽  
Method Comparison ◽  
Armed Forces ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
The Mean

Abstract Objective The purpose of this study was to compare the performances of and evaluate the agreement among glycated hemoglobin values analyzed by using National Glycohemoglobin Standardization Program-certified and International Federation of Clinical Chemistry-standardized analyzers. This cross-sectional study was conducted at the Armed Forces Institute of Pathology, Department of Chemical Pathology from March 2019 to May 2019. Methods Glycated hemoglobin (HbA1c) was measured in the blood specimens from 100 patients on an ADVIA 1800 by a turbidimetric inhibitory immunoassay (TINIA), Sebia instrument by electrophoresis, and Bio-Rad Variant II Turbo system by high-performance liquid chromatography (HPLC). Quantitative variables were calculated as the mean ± standard deviation (SD). Precision and method comparisons were carried out according to Clinical and Laboratory Standards Institute recommendations. The results obtained from each analyzer were compared by correlation analysis. Method comparison was done by linear regression and Bland-Altman plots using the SPSS software version 24. Results The mean ± SD HbA1c values from TINIA, electrophoresis, and HPLC were 7.188% ± 1.89%, 7.164% ± 1.866%, and 7.160% ± 1.85%, respectively. The between-run coefficients of variation for TINIA, electrophoresis, and HPLC were 0.64%, 0.61%, and 0.60%, respectively. All 3 showed good correlation (TINIA, R2 = .994, P = .00; electrophoresis, R2 = .992, P = 0.00; and HPLC, R2 = .994, P = 0.00). Conclusion The good clinical agreements of HbA1c and strong correlations between analyzers indicate that these analyzers can be used interchangeably.

Haemoglobin variants may cause significant differences in haemoglobin A1c as measured by high-performance liquid chromatography and enzymatic methods in diabetic patients: a cross-sectional study

2016 ◽  
Vol 54 (4) ◽  
pp. 432-437 ◽  
Author(s):  
Shuichi Otabe ◽  
Hitomi Nakayama ◽  
Tsuyoshi Ohki ◽  
Eri Soejima ◽  
Yuji Tajiri ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Globin Gene ◽  
Cross Sectional Study ◽  
Enzymatic Assay ◽  
Diabetic Patients ◽  
Haemoglobin A1c ◽  
Cross Sectional ◽  
Hb Toranomon

Background We aimed to determine whether the discrepancy between haemoglobin A1c values determined by high-performance liquid chromatography and enzymatic haemoglobin A1c measurements in diabetic patients was clinically relevant. Methods We randomly recruited 1421 outpatients undergoing diabetic treatment and follow-up who underwent at least three haemoglobin A1c measurements between April 2014 and March 2015 at our clinic. In 6369 samples, haemoglobin A1c was simultaneously measured by HA-8160 and MetaboLead (enzymatic assay), and the values were compared. Results haemoglobin A1c measurements by high-performance liquid chromatography and enzymatic assay were strongly correlated (correlation coefficient: 0.9828, linear approximation curve y = 0.9986x − 0.2507). Mean haemoglobin A1c (6.8 ± 1.0%) measured by high-performance liquid chromatography was significantly higher than that measured by enzymatic assay (6.5 ± 1.0%, P < 0.0001). During the sample processing, four (0.3%) subjects presented consistently lower haemoglobin A1c values (<0.7%) by high-performance liquid chromatography than those from enzymatic assay. Of these, three had Hb Toranomon [β112 (G14) Cys→Trp]. The fourth had Hb Ube-2 [α68 (E17) Asn→Asp]. One other subject presented consistently higher haemoglobin A1c values (>1%) by high-performance liquid chromatography than those from enzymatic assay and was diagnosed with a −77 (T > C) mutation in the δ-globin gene. These unrelated asymptomatic subjects had normal erythrocyte profiles, without anaemia. Conclusions We showed that haemoglobin A1c values measured by high-performance liquid chromatography were significantly higher than those measured by enzymatic assay in diabetic subjects. However, when an oversized deviation (>0.7%) between glycaemic control status and haemoglobin A1c is apparent, clinicians should check the methods used to measure haemoglobin A1c and consider the possible presence of a haemoglobin variant.

scholarly journals Estimation of Glycated Haemoglobin by Nephelometry, Ion Exchange Resin and High Performance Liquid Chromatography: A Cross-sectional Study

2020 ◽  
Author(s):  
Mangalore Balakrishna Prathima ◽  
Shridhar Reshma ◽  
. Sushith ◽  
Prathiksha Shetty ◽  
D'Sa Janice ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Gold Standard ◽  
High Performance ◽  
Glycated Haemoglobin ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
The Mean ◽  
R Value

Introduction: Diabetes mellitus cases are continually rising all over the world. Glycated haemoglobin (HbA1c) used as a diagnostic test to measure long-term average glycaemic control in diabetic patients. Aim: To assess the precision and reproducibility of the Ion Exchange Column Chromatography Resin (IECR) method and nephelometry in comparison to High-Performance Liquid Chromatography (HPLC) with respect to the estimation of HbA1c. Materials and Methods: A cross-sectional comparative study was conducted on 50 blood samples, collected from the diabetic subjects at the Department of Biochemistry and their HbA1c values were estimated by HPLC based BioRad D-10, nephelometry and IECR techniques. HPLC was used as a gold standard method, to evaluate the sensitivity and specificity of nephelometry and IECR techniques. Data were expressed as mean±standard deviation and intraclass Correlation Coefficient and Pearson correlation were calculated. Results: The mean age of the study subjects was 53.06±7.67 years. The mean plasma HbA1c levels were 8.16±2.9, 7.62±2.5 and 7.84±2.5 and mean estimated Average Glucose (eAG) was 187.00±84.4, 172.0±76.8, and 289.46±199.9 by HPLC, Nephelometry and IECR, respectively. Compared with HPLC, nephelometry had excellent correlation (r-value 0.925); p<0.001) and IECR (r-value 0.869; p<0.001). Conclusion: Nephelometry and IECR both had better performance and showed a greater concordance with gold standard HPLC. Therefore, nephelometry and IECR can be used as an alternative assay for HbA1c estimation.

Occurrence of Aflatoxin M1 in Cheeses from the South of Spain

1996 ◽  
Vol 59 (8) ◽  
pp. 898-900 ◽  
Author(s):  
Mª JOSÉ BARRIOS ◽  
Mª JESÚS GUALDA ◽  
J. M. CABANAS ◽  
L. M. MEDINA ◽  
R. JORDANO
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Aflatoxin M1 ◽  
The South ◽  
Different Types ◽  
The Mean

Thirty-five samples of commercial cheeses, 9 fresh, 9 semicured or semiripened and 17 ripened made with different types of milk (cow, ewe, goat and mixtures of milk of various species) produced in the South of Spain were analyzed for the presence of aflatoxin M1 (AFM1) by high-performance liquid chromatography, In 16 of the 35 samples (45.71%) the presence of AFM1 was detected in concentrations ranging between 20 and 200 ng/g of cheese, In the positive cases, the mean levels of AFM1 were 105.33 ng/g in ripened cheeses, 73.80 ng/g in semiripened cheeses and 42.60 ng/g in fresh cheeses.

scholarly journals ANALISIS KADAR BENZOAT DAN SORBAT PADA SAUS SAMBAL KEMASAN YANG DIJUAL DI PASAR BARU BEKASI DENGAN METODE HPLC

2020 ◽  
Vol 3 (1) ◽  
pp. 22-27
Author(s):  
Maulidya Mitha Rianto ◽  
Elfira Mayasari ◽  
Siti Nurfajriah
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cross Sectional ◽  
Hplc Data

Pendahuluan: Bahan Tambahan Pangan (BTP) digunakan secara luas oleh masyarakat. Bahan Tambahan Pangan (BTP) yang banyak digunakan dalam saus sambal adalah pengawet seperti benzoat dan sorbat. Penggunaan pengawet pada bahan pangan tidak boleh melebihi ambang batas yang sudah ditentukan karena dapat memberikan efek bagi kesehatan, seperti benzoat yang dapat menimbulkan reaksi alergi dan bersifat karsinogenik karena adanya benzene serta sorbat yang dapat menyebabkan mutasi gen bila dikombinasikan dengan asam askorbat. Penelitian ini bertujuan untuk mengetahui kadar benzoat dan sorbat pada saus sambal kemasan yang dijual di Pasar Baru Bekasi dan melihat kesesuaian kadar pengawet dengan ambang batas yang sudah ditentukan. Metode: Metode penelitian yang digunakan adalah deskriptif kuantitatif dengan pendekatan cross-sectional. Kadar benzoat dan sorbat diperiksa menggunakan metode High Performance Liquid Chromatography (HPLC). Data kuantitatif yang diperoleh dari hasil pengukuran dianalisis dengan membandingkan kadar maksimum benzoat dan sorbat yang diperbolehkan pada saus menurut Peraturan Kepala BPOM No. 36 Tahun 2013, yaitu 1000 mg/kg. Kadar benzoat dan sorbat yang melebihi ambang batas menandakan bahwa saus sambal tersebut tidak boleh untuk dikonsumsi. Hasil: Hasil penelitian menunjukkan bahwa seluruh 100% sampel mengandung benzoat melebihi kadar maksimum yang telah ditetapkan dan 50% sampel mengandung sorbat dengan kadar di bawah ambang batas yang telah ditentukan. Kesimpulan: Berdasarkan penelitian yang telah dilakukan, saran yang dapat diberikan untuk penelitian selanjutnya adalah melakukan analisis kadar benzoat dan sorbat pada sampel bahan pangan yang lain mengingat bahwa benzoat dan sorbat sering digunakan sebagai kombinasi pengawet dan diperbolehkan untuk digunakan dalam bahan pangan.

scholarly journals Changes in the contents of kaempherol, quercetin and L-ascorbic acid in sea buckthorn berries during maturation

2000 ◽  
Vol 9 (1) ◽  
pp. 17-22 ◽  
Author(s):  
N. JEPPSON ◽  
G. XIANGQUN
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Sea Buckthorn ◽  
Maturation Stages ◽  
The Mean ◽  
Over Time

The contents of kaempherol, quercetin and L-ascorbic acid in sea buckthorn berries were measured at different maturation stages using High Performance Liquid Chromatography (HPLC) methods. The content of ascorbic acid decreased over time with significant differences between sampling dates for the five cultivars studied. The mean decrease was 25% in 19 days, from 1.48 to 1.10 g kg-1. Quercetin decreased whereas kaempherol increased during maturation. Among three studied cultivars, the decrease in quercetin was significant (from 0.028 to 0.014 g kg-1) in 'Otradnaja', where as the increase (from 0.012 to 0.016 g kg-1) in kaempherol was significant in the others two, 'Prozratnaja' and 'Gibrid Pertjik'.;

Leaching from the stratum corneum does not explain the previously reported elevated potassium ion concentration in sweat

2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Michael J. Buono ◽  
Michael Stone ◽  
Daniel T. Cannon
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stratum Corneum ◽  
High Performance ◽  
Isopropyl Alcohol ◽  
Ion Concentration ◽  
Distilled Water ◽  
Artificial Sweat ◽  
The Mean ◽  
Skin Cleansing

AbstractThe purpose of this study was to determine if KTwelve (six female, six male) healthy volunteers participated in this study. After thorough skin cleansing and preparation with isopropyl alcohol and high-performance liquid chromatography-grade distilled water, three sites were chosen and a 50 μL drop of artificial sweat was pipetted directly onto the skin. The artificial sweat had a [KThe mean (±SD) [KThese results do not support the hypothesis that significant K

scholarly journals Identification of Phenolic Acids and Flavonoids in Monofloral Honey from Bangladesh by High Performance Liquid Chromatography: Determination of Antioxidant Capacity

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammed Moniruzzaman ◽  
Chua Yung An ◽  
Pasupuleti Visweswara Rao ◽  
Mohammad Nurul Islam Hawlader ◽  
Siti Amirah Binti Mohd Azlan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Sugar Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
The Mean

The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. A total of five different phenolic acids were identified, with the most abundant being caffeic acid, benzoic acid, gallic acid, followed by chlorogenic acid and trans-cinnamic acid. The flavonoids, kaempferol, and catechin were most abundant, followed by myricetin and naringenin. The mean moisture content, total sugar content, and color characteristics of the honey samples were 18.36 ± 0.95%, 67.40 ± 5.63 g/100 g, and 129.27 ± 34.66 mm Pfund, respectively. The mean total phenolic acids, total flavonoid content, and proline content were 199.20 ± 135.23, 46.73 ± 34.16, and 556.40 ± 376.86 mg/kg, respectively, while the mean FRAP values and DPPH radical scavenging activity were 327.30 ± 231.87 μM Fe (II)/100 g and 36.95 ± 20.53%, respectively. Among the different types of honey, kalijira exhibited the highest phenolics and antioxidant properties. Overall, our study confirms that all the investigated honey samples are good sources of phenolic acids and flavonoids with good antioxidant properties.

An evaluation of the Abbott IMx sirolimus assay in relation to a high-performance liquid chromatography-ultraviolet method

2005 ◽  
Vol 42 (5) ◽  
pp. 394-397 ◽  
Author(s):  
Roger N Johnson ◽  
Russell Sargon ◽  
Gerald Woollard ◽  
James Davidson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Immunosuppressive Agent ◽  
Hplc Method ◽  
Transplant Patients ◽  
Coefficients Of Variation ◽  
Microparticle Enzyme Immunoassay ◽  
The Mean ◽  
Blood Specimens

Background: Sirolimus is an immunosuppressive agent used after organ transplantation. Monitoring its concentration is clinically relevant, but current methods of measurement using high-performance liquid chromatography (HPLC) are demanding. An immunoassay that uses readily available equipment offers a more convenient alternative. Methods: Blood specimens from transplant patients were assayed for sirolimus by an existing HPLC method with ultraviolet (UV) detection and by the Abbott microparticle enzyme immunoassay (MEIA). Within-run and between-day precision was assessed with duplicate analyses. Accuracy was assessed by comparison of results from the MEIA and HPLC assays, and from recovery experiments. Specificity was tested in specimens from patients taking alternative immunosuppressants. Results: Using specimens with sirolimus concentrations between 3.3 and 39.3 µg/L, within-batch and between-batch coefficients of variation (CVs) were 6.8% and 6.3%, respectively. Using control specimens with mean concentrations of 4.1, 12.1 and 25.4 µg/L, the respective CVs were 8.6%, 3.1% and 3.8 %. Using a Passing-Bablok regression technique, the regression equation was MEIA = 14; 1.26 x HPLC-1.97; the mean bias was 0.96 µg/L. Specimens from patients on cyclosporin and tacrolimus as the sole immunosuppressant had apparent sirolimus concentrations of <1 µg/L. Conclusion: The MEIA assay for sirolimus provides clinically useful information within an accessible format.

Determination of β -Sitosterol with Chemical Course and Material Applications in Jatropha Seed Oil by High Performance Liquid Chromatography

2012 ◽  
Vol 577 ◽  
pp. 69-72 ◽  
Author(s):  
Shu Yu Liu ◽  
Anaerguli Maihemuti
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Seed Oil ◽  
Reversed Phase ◽  
Analytical Recovery ◽  
Relative Standard ◽  
The Mean ◽  
Jatropha Seed

A simple and rapid high performance liquid chromatography (HPLC) assay was developed to identify and measure theβ-sitosterol with chemical course and material applications in jatropha seed oil. The stigmasterol was isolated with a good selectivity by HPLC employing reversed phase C18 columns. The components were separated by mobile phase of methanol-water (99/1, v/v) and detected at 205nm. The quantitation of the stigmasterol was reproducible and the method relative standard deviation is 1.1%. The mean analytical recovery was 96.2%.

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