scholarly journals Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

2018 ◽  
Vol IX (18) ◽  
pp. 5-11 ◽  
Author(s):  
Mohammad MAHMOODI ◽  
Kian Pahlevan AFSHARI ◽  
Hamid Reza SEYEDABADI ◽  
Mehran ABOOZARI
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Mitochondrial Genes ◽  
12S Rrna

Phylogeny of Dorippoidea (Crustacea : Decapoda : Brachyura) inferred from three mitochondrial genes

2009 ◽  
Vol 23 (3) ◽  
pp. 223 ◽  
Author(s):  
Y. W. Sin ◽  
Joelle C. Y. Lai ◽  
Peter K. L. Ng ◽  
K. H. Chu
Keyword(s):  
Molecular Markers ◽  
Cytochrome C ◽  
16S Rrna ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Relationships ◽  
Phylogenetic Trees ◽  
Mitochondrial Genes ◽  
12S Rrna ◽  
Generic Relationships ◽  
I Gene

The phylogenetic relationships between 10 of 13 genera of crabs from the superfamily Dorippoidea were investigated using mitochondrial 16S rRNA, 12S rRNA and cytochrome c oxidase subunit I gene sequences. The resultant phylogenetic trees based on the three molecular markers support the division of Dorippidae and Ethusidae as monophyletic families within the Dorippoidea. The inferred inter-generic relationships within Dorippidae concur with groupings based on the overall morphology of the carapace and structures of the male first pleopods.

Description and phylogenetic relationships of a new trans-Andean species of Elachistocleis Parker 1927 (Amphibia, Anura, Microhylidae)

Zootaxa ◽  
2020 ◽  
Vol 4779 (3) ◽  
pp. 323-340
Author(s):  
JUAN C. SÁNCHEZ-NIVICELA ◽  
PEDRO L. V. PELOSO ◽  
VERÓNICA L. URGILES ◽  
MARIO H. YÁNEZ-MUÑOZ ◽  
YERKA SAGREDO ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
South America ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Mitochondrial Genes ◽  
12S Rrna ◽  
The Andes ◽  
Southern Ecuador ◽  
A New Species

Elachistocleis is a Neotropical genus of microhylid frogs with 18 species, most of which occur east of the Andes in South America. Here, we present a new phylogeny of Gastrophryninae and describe and name a new species of Elachistocleis from southern Ecuador—the first to be found west of the Andes and also the first from Ecuador. Our phylogeny is based on DNA sequences of the mitochondrial genes 12S rRNA, 16S rRNA, COI, and the nuclear genes BDNF, cmyc2, H3A, 28S, SIA1, and Tyr. Elachistocleis araios sp. n., is the sister species of all other Elachistocleis. The finding of this taxon highlights the probability of the existence of more Elachistocleis species west of the Andes. 

Genetic and morphometric categorization ofTaenia ovisfrom Sheep in Iran

Parasitology ◽  
2018 ◽  
Vol 146 (5) ◽  
pp. 563-568 ◽  
Author(s):  
Sima Rostami ◽  
Reza Salavati ◽  
Robin N. Beech ◽  
Zahra Babaei ◽  
Mitra Sharbatkhori ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Correlation Coefficients ◽  
Morphological Characters ◽  
Mitochondrial Genes ◽  
Rrna Genes ◽  
12S Rrna ◽  
Length Variation ◽  
Rostellar Hook ◽  
Separate Species ◽  
Interclass Correlation

AbstractLittle is known about the genetic and morphological characters ofTaenia ovis. The purpose of the present study was to characterize sheep isolates ofT. ovisusing rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens ofCysticercus oviswere collected from 18 slaughterhouses in Iran. The mean ±s.d.for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4µm, respectively. CO1 and 12S rRNA sequence analysis showed 11 and nine haplotypes, respectively. The level of pairwise nucleotide variations between individual haplotypes of CO1 and 12S rRNA genes were 0.3–1.1 and 0.2–1.0%, respectively. Level of nucleotide variation in CO1 and 12S rRNA betweenT. ovishaplotypes from present study and eight otherTaeniaspecies was found to be 11.3–17.8 and 5.3–16.3%, respectively. Phylogenetic analysis clustered allT. ovisisolates into a single clade comprised of the all CO1 and 12S rRNA haplotypes. CO1 nucleotide difference betweenT. ovis ovisandT. asiaticawas 13.6% that is lesser than the corresponding difference betweenT. ovis ovisandT. ovis krabbei, warranting the designation of two separate species asT. ovisandT. krabbei. Interclass correlation coefficients showed that there was no significant association between rostellar hook length variation and the variability of the mitochondrial genes.

scholarly journals Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Mohammad Mahmoodi ◽  
Kian Pahlevan Afshari ◽  
Hamid Reza Seyedabadi ◽  
Mehran Aboozari
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Pcr Amplification ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Conservation Strategies ◽  
12S Rrna ◽  
Rrna Gene ◽  
Salting Out ◽  
The Rich

Phylogenetic relationships and genetic variation in Iranian Afshari sheep breed were analyzed using 12S rRNA and 16S rRNA gene sequences. The genomic DNA was isolated by salting out method and amplified 12S rRNA and 16S rRNA genes using PCR method. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 859 and 1053 bp lengths, respectively. Sequence analysis was performed using BioEdit software. Phylogenetic tree was constructed using MEGA software. Phylogenetic analysis of haplotype in the combination with the sheep from GenBank showed that Iranian Afshari sheep made a close to the Australian sheep cluster. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.

scholarly journals Diagnosis of Fulminant Pneumonia Caused by Legionella pneumophila Serogroup 8 with the Sequence Analysis of the 16S rRNA Gene

2011 ◽  
Vol 225 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Toshinori Kawanami ◽  
Kazuhiro Yatera ◽  
Kazumasa Fukuda ◽  
Kei Yamasaki ◽  
Masamizu Kunimoto ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Legionella Pneumophila ◽  
Rrna Gene ◽  
The 16S Rrna Gene

scholarly journals Kaistia terrae sp. nov., isolated from a wetland in Korea

2010 ◽  
Vol 60 (4) ◽  
pp. 949-952 ◽  
Author(s):  
Soo-Jin Kim ◽  
Hang-Yeon Weon ◽  
Yi-Seul Kim ◽  
Rangasamy Anandham ◽  
Seung-Hee Yoo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis

An ivory-coloured bacterium, designated strain 5YN7-3T, was isolated from a wetland, Yongneup, Korea. Cells of the strain were aerobic, Gram-stain-negative, non-motile and short rods. 16S rRNA gene sequence analysis demonstrated that strain 5YN7-3T belongs to the order Rhizobiales of the class Alphaproteobacteria and is closely related to Kaistia soli 5YN9-8T (97.8 %), Kaistia granuli Ko04T (97.6 %) and Kaistia adipata Chj404T (97.4 %). Strain 5YN7-3T showed DNA–DNA hybridization values of 28, 22 and 35 % with K. granuli Ko04T, K. soli 5YN9-8T and K. adipata Chj404T, respectively. The major fatty acids were C18 : 1 ω7c (51.2 %), C19 : 0 cyclo ω8c (25.0 %), C18 : 0 (12.9 %) and C16 : 0 (10.8 %) (>10 % of total fatty acids). Ubiquinone-10 was the major isoprenoid quinone and the DNA G+C content was 66.5 mol%. The phenotypic characteristics in combination with 16S rRNA gene sequence analysis and DNA–DNA hybridization data clearly define strain 5YN7-3T as a novel species of the genus Kaistia, for which the name Kaistia terrae sp. nov. is proposed. The type strain is 5YN7-3T (=KACC 12910T =DSM 21341T).

scholarly journals Parabacteroides johnsonii sp. nov., isolated from human faeces

2007 ◽  
Vol 57 (2) ◽  
pp. 293-296 ◽  
Author(s):  
Mitsuo Sakamoto ◽  
Maki Kitahara ◽  
Yoshimi Benno
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Human Faeces ◽  
Gene Sequence Analysis

A bacterial strain isolated from human faeces, M-165T, was characterized in terms of its phenotypic and biochemical features, cellular fatty acid profile, menaquinone profile and phylogenetic position (based on 16S rRNA gene sequence analysis). A 16S rRNA gene sequence analysis showed that the isolate was a member of the genus Parabacteroides. Strain M-165T was closely related to Parabacteroides merdae strains, showing 98 % sequence similarity. The strain was obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative, rod-shaped and was able to grow on media containing 20 % bile. Although the phenotypic characteristics of the strain M-165T were similar to those of P. merdae, the isolate could be differentiated from P. merdae by means of API 20A tests for l-arabinose and l-rhamnose fermentation. DNA–DNA hybridization experiments revealed the genomic distinctiveness of the novel strain with respect to P. merdae JCM 9497T (⩽60 % DNA–DNA relatedness). The DNA G+C content of the strain is 47.6 mol%. On the basis of these data, strain M-165T represents a novel species of the genus Parabacteroides, for which the name Parabacteroides johnsonii sp. nov. is proposed. The type strain is M-165T (=JCM 13406T=DSM 18315T).

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