In vitro Antiglycation, Antioxidant Properties of Coleus forskohlii ''Balady'' Leaves and Stem and their Antioxidant Enzyme Activities

2017 ◽  
Vol 19 (3) ◽  
pp. 1-11 ◽  
Author(s):  
Yaaser Almulaiky ◽  
Abudukadeer Kuerban ◽  
Faisal Aqlan ◽  
Saeed Alzahrani ◽  
Mohammed Baeshen ◽  
...  
2009 ◽  
Vol 87 (8) ◽  
pp. 610-616 ◽  
Author(s):  
K. Govindan Nevin ◽  
Thankappan Rajamohan

Because coconut oil extracted by wet process (virgin coconut oil, VCO) is gaining popularity among consumers, this study was conducted to evaluate VCO compared with coconut oil extracted by dry process (copra oil, CO) for their influence on lipid parameters, lipid peroxidation, and antioxidant status in rats coadministered with cholesterol. VCO, CO, and cholesterol were fed in a semi-synthetic diet to 24 male Sprague–Dawley rats for 45 days. After the experimental period, lipid and lipid peroxide levels and antioxidant enzyme activities were observed. Chemical composition and antioxidant properties of the polyphenolic fraction from VCO and CO were also analyzed. The results showed that lipid and lipid peroxide levels were lower in VCO-fed animals than in animals fed either CO or cholesterol alone. Antioxidant enzyme activities in VCO-fed animals were comparable with those in control animals. Although the fatty acid profiles of both oils were similar, a significantly higher level of unsaponifiable components was observed in VCO. Polyphenols from VCO also showed significant radical-scavenging activity compared with those from CO. This study clearly indicates the potential benefits of VCO over CO in maintaining lipid metabolism and antioxidant status. These effects may be attributed in part to the presence of biologically active minor unsaponifiable components.


ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aman Verma ◽  
C. P. Malik ◽  
V. K. Gupta

This paper deals with the in vitro effects of brassinosteroids (BRs) on growth in the form of multiple shoots, chlorophyll content, Hill reaction activity (HRA), activities of catalase (CAT), peroxidase (POX), polyphenol oxidase (PPX), and ascorbate peroxidase (APX) in Arachis hypogaea L. genotypes (M-13 and PBS24030). In vitro impact of BR on shoot multiplication potential was found to be the best at 1 mL L−1 with BA (3 mg L−1) in both the cultivars. Flowering was observed in PBS24030 on the medium containing 2.0 mL L−1 BR with 3 mg L−1 BA. Rhizogenesis was noticed in the presence of BR alone. Total chlorophyll content and HRA were highest at 2.0 mL L−1 with BA in M-13 and 1.0 mL L−1 with BA in PBS24030. Antioxidant enzyme activities were increased in the presence of BR whether alone or in combination with BA in both the cultivars. However, progressive decline was observed in case of MDA content. The results obtained in the study clearly indicated not only the in vitro establishment of groundnut cultivars in the presence of BR alone and in combination with BA but also its effect on various growth promotory physiological parameters.


2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Amol L. Shirfule ◽  
Venkatesh Racharla ◽  
S. S. Y. H. Qadri ◽  
Arjun L. Khandare

Gokshuradi Yog (GY)is a polyherbal ayurvedic formulation used traditionally for several decades in India for the treatment of urolithiasis. The aim of the present study was to determine the underlying mechanism ofGYaction in the management of calcium oxalate urolithiasis. The effect ofGokshuradi polyherbal aqueous extracts(GPAEs) was studied on various biochemical parameters involved in calcium oxalate formation by employingin vitroandin vivomethods.GPAEexhibited significant antioxidant activity against 1, 1-diphenyl-2-picrylhydrazyl free radical and inhibited lipid peroxidation in thein vitroexperiments. The rat model of urolithiasis induced by 0.75% ethylene glycol (EG) and 1% ammonium chloride (AC) in water caused polyuria, weight loss, impairment of renal function, and oxidative stress and decreased antioxidant enzyme activities in untreated control groups. However,GPAE-(25, 50, and 100 mg/kg) treated groups caused diuresis accompanied by a saluretic effect and revealed significant increase in antioxidant enzyme activities along with decreased oxalate synthesizing biochemical parameters at higher doses. This study revealed the antiurolithic effect ofGPAEmediated possibly through inhibiting biochemical parameters involved in calcium oxalate formation, along with its diuretic and antioxidant effects, hence supporting its use in the treatment of calcium oxalate urolithiasis.


2009 ◽  
Vol 64 (11-12) ◽  
pp. 859-863 ◽  
Author(s):  
Shun Gao ◽  
Rui Yan ◽  
Jun Wu ◽  
Fu-li Zhang ◽  
Sheng-hua Wang ◽  
...  

Jatropha curcas embryos were grown in vitro to observe the effects of lead on cotyledon responses. The cotyledon biomass increased initially and then decreased with increasing lead concentration. The SOD activity increased gradually up to 200 μM and then decreased. The POD activity showed a similar trend. The CAT activity was increased at all lead concentrations, the highest activity being observed at 200 μM. However, the PAL activity was inhibited significantly except for 100 μM. Anaylsis by electrophoresis suggested a significant correlation between lead concentration and patterns of SOD, POD and CAT isoenzymes, and these results were consistent with changes of the antioxidant enzyme activities as assayed in solution.


2019 ◽  
Vol 16 (04) ◽  
pp. 725-729
Author(s):  
Tuğba Gür ◽  
Fatih Karahan ◽  
Halit Demir ◽  
Canan Demir

Superoxide dismutase (SOD) and catalase enzyme (CAT) activities with strong antioxidant properties were determined in cherry fruits obtained from different regions such as Aegean, Mediterranean and Marmara. The cherry fruit extract was prepared and some antioxidant activities were determined. Cherry (prunus avium) is a fruit belonging to the family of rosaceae. Its homeland is asia minor. Many varieties are grown in Turkey. There are more than a hundred culture forms grown in north america with temperate regions of europe and asia. Its body is in the form of a flat-shell tree. Cherry is a fruit rich in vitamin C. They do not contain fat and cholesterol. It contains essential minerals such as fiber, vitamin A, iron, calcium, protein as well as abundant potassium. Red cherries also contain melatonin, which helps combat harmful toxins. Due to its antioxidant properties, it has many benefits such as prevention of some types of cancer, reduction of inflammation, prevention of gout and removal of muscle pain. For this purpose, it is aimed to determine some enzyme activities which are thought to be found in cherry fruit. In this study, antioxidant enzyme activities in cherry fruit were determined by spectrophotometric method. Additonaly the findings were analyzed by using multidimensional statistical methods and the results were discussed in a multidimensional manner. It is obtained that the highest catalase enzyme activity was determined in the Aegean region (4.330 U/L), while the highest superoxide dismutase enzyme activity was found in the Mediterranean region (7.176 U/L).


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