Prevalence of Multiple Antibiotic Resistant Escherichia coli Serotypes in Cow Raw Milk Samples and Traditional Dairy Products in Osun State, Nigeria

2015 ◽  
Vol 5 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Thonda Abike ◽  
Oluduro Olufunke ◽  
Kolawole Oriade
1979 ◽  
Vol 42 (12) ◽  
pp. 950-953 ◽  
Author(s):  
LESTER HANKIN ◽  
GEORGE H. LACY ◽  
GEORGE R. STEPHENS ◽  
WALTER F. DILLMAN

Raw milk samples were examined for number and percentage of bacteria resistant to seven antibiotics: penicillin, ampicillin, chloramphenicol, neomycin sulfate, polymyxin B sulfate, tetracycline and streptomycin sulfate. A significant negative correlation was found between the total aerobic count of the milk sample and the concentration (above 5 or 10% of the total count) of bacteria in each milk resistant to each of the antibiotics tested. Three of 42 gram-negative isolates were capable of transferring their antibiotic resistance to Escherichia coli. Substantial numbers of antibiotic-resistant bacteria in raw milk were found and some survived pasteurization. Inspection of farms failed to indicate a relationship between farm practices or use of antibiotics in feed or as pharmaceuticals and number of antibiotic-resistant bacteria in the raw milk.


2014 ◽  
Vol 38 (2) ◽  
pp. 9-16
Author(s):  
Najim Hadi Najim

     Milk and dairy products are fundamental components in the human diet and may be the principle way for entrance of Aflatoxin M1 (AFM1) in to the human body. All milk and dairy products samples were tested for the occurrence of AFM1 by the competitive ELISA technique. Out of 32 bovine raw milk samples that were collected from eight villages around Baghdad province, 32 samples (100 %) were contaminated with AFM1 ranging from 0.15 to 86.96ng/kg with mean value of 42.37±26.07 ng/kg, of which 17 samples were contaminated with concentrations < 50 ng/kg and 15 samples exceeded the maximum acceptable level of AFM1 in milk (50 ng/kg) imposed by the European legislation. The raw milk samples belonged to animals fed with composite and stored fodder as in Althahab Alabiadh, Radhwaniya and Fadhaliya villages had higher significantly AFM1 concentrations over all the other five villages (Grazing feed). All 32 (100%) locally produced soft white cheese samples analyzed were contaminated with AFM1 ranging from 31.84 to 89.44 ng/kg with the mean value of 59.92±17.03 ng/kg. Out of 32 locally produced yoghurt samples analyzed, 32 samples (100%) were contaminated with AFM1 ranging from 0.16 to 42.74 ng/kg with the mean value of 16.92±11.55 ng/kg. Thirty samples (100%) of the examined 30 imported UHT milk samples that were collected from different commercial companies in the province of Baghdad presented significantly  high contamination level with AFM1 that were found to range from 0.18 to 85.66 ng/kg.


2008 ◽  
Vol 71 (5) ◽  
pp. 1023-1027 ◽  
Author(s):  
R. N. COBBOLD ◽  
M. A. DAVIS ◽  
D. H. RICE ◽  
M. SZYMANSKI ◽  
P. I. TARR ◽  
...  

A survey for Shiga toxigenic Escherichia coli in raw milk and beef was conducted within a defined geographic region of the United States. Prevalence rates based on detection of Shiga toxin gene (stx) were 36% for retail beef, 23% for beef carcasses, and 21% for raw milk samples, which were significantly higher than were Shiga toxigenic E. coli isolation rates of 7.5, 5.8, and 3.2%, respectively. Seasonal prevalence differences were significant for stx positivity among ground beef and milk samples. Distribution of stx subtypes among isolates varied according to sample type, with stx1 predominating in milk, stx2 on carcasses, and the combination of both stx1 and stx2 in beef. Ancillary virulence markers eae and ehx were evident in 23 and 15% of isolates, respectively. Pulsed-field gel electrophoresis demonstrated associations between food isolates and sympatric bovine fecal, and human clinical isolates. These data demonstrate that non-O157 Shiga toxigenic E. coli is present in the food chain in the Pacific Northwest, and its risk to health warrants critical assessment.


Author(s):  
E. Seker ◽  
H. Yardimci

Three hundred rectal faecal samples and 213 raw milk samples obtained from the tanks and containers were examined using standard cultural methods. Escherichia coli O157:H7 was isolated from 11 (3.7 %) of 300 faecal samples and 3 (1.4 %) of 213 raw milk samples. It was determined that 8 (73 %) of E. coli O157:H7 strains isolated from faecal samples originated from water buffaloes younger than 2 years of age and 3 (27 %) from 2-year-old and older water buffaloes. This is the 1st isolation of Escherichia coli O157:H7 from faecal and milk samples of water buffaloes in Turkey.


2018 ◽  
Vol 114 ◽  
pp. 141-144 ◽  
Author(s):  
Fateme Akrami-Mohajeri ◽  
Zahra Derakhshan ◽  
Margherita Ferrante ◽  
Negar Hamidiyan ◽  
Meysam Soleymani ◽  
...  

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