Effect of Combined Inoculation of Phosphate Solubilizing Bacteria and Endomycorrhizae Fungi on Microbial Population and NPK Uptake by Wheat (Triticum aestivum L.) Crop

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Ilmiasari Y, Yosita R, Annisa RM, Sari AW, Andayani AP, Yulianti DM. 2019. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome. Biodiversitas 20: 3315-3321. Palm oil bunches and pineapple rhizomes are abundant in Indonesia, especially in Lampung Province due to widespread agro-industrial company and they are potential to be used as sources for liquid organic fertilizer. This study was aimed to reveal microbial population within liquid organic fertilizer (LF) which was developed from palm oil bunches (PB) and pineapple rhizome (PR) and their role as phosphate solubilizing bacteria (PSB). The capability to solubilize phosphate was recorded from the clear zone area around the bacterial colony which was cultivated on Pikovskaya’s agar medium. The LF was prepared in three kinds of conditions, namely aerobic, anaerobic, and facultative an aerobic. Isolation was performed every 3 days until 24 days after incubations. The results showed that microbial population in the LF from PR was significantly higher than those developed from PB. Totally, 791 bacterial isolates were obtained from the LF; 490 isolates were achieved from PR (153 isolates from aerobic, 188 isolates from facultative anaerobic, 149 from anaerobic conditions) and 301 isolates were collected from PB (96 isolates from aerobic, 112 isolates from facultative anaerobic, 93 isolates from anaerobic conditions). In the case of isolates that were gained from PB, 166 isolates (55.15%) showed capability to degrade phosphate and 135 isolates (44.85%) did not show any capability as PSB. As for isolates obtained from PR, 269 isolates (54.90%) had capability to solubilize phosphate, meanwhile, the other 221 isolates (45.10%) did not show any capability to solubilize phosphate. Among166 isolates of PSB from PB, 60 isolates had very low capability, 55 isolates had low capability, 34 isolates had medium capability, 10 isolates had high capability and only 7 isolates had very high capability. Meanwhile, from 269 isolates of PSB obtained from PR, 135 isolates had very low capability to solubilize phosphate, 84 isolates had low capability to solubilize phosphate, 32 had medium capability to solubilize phosphate, 14 isolates had high capability to solubilize phosphate and 4 isolates produced very high capability as PSB. In total, 11 chosen isolates were selected from LF developed from both PB (7 isolates) and PR (4 isolates). Therefore, palm oil bunches and pineapple rhizomes contained PSB that can be used as LF.

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Effect of herbicide combinations on weed dynamics, nutrient removal and yield of Wheat (Triticum aestivum L.) under north western plain zone

International Journal of Agricultural Invention ◽

10.46492/ijai/2017.2.1.3 ◽

2017 ◽

Vol 2 (01) ◽

pp. 10-15

Author(s):

Adesh Singh

Keyword(s):

Triticum Aestivum ◽

Weed Control ◽

Triticum Aestivum L ◽

Research Centre ◽

Metsulfuron Methyl ◽

Npk Uptake ◽

Weed Dynamics ◽

Crop Research ◽

North Western ◽

Clodinafop Propargyl

A field experiment was conducted to study the effect of different weed control practices on weed dyanamics, nutrient uptake and yield of wheat (Triticum aestivum L.) in rabi seasons of 2010-11 and 2011-12 at Crop Research Centre of SVPUAandT, Meerut. The treatments comprising pre-emergence application of pendimethalin @ 1000 g/ha, post emergence application of isoproturon @1200 g/ha, isoproturon+2,4-D @ 1200+600 g/ha, isoproturon+metsulfuron methyl @ 1200+4 g/ha, fenoxaprop -p-ethyl @ 80 g/ha, fenoxa prop -p-ethyl +2,4-D @ 80+600 g/ha, fenoxa prop -p-ethyl +metsulfuron methyl @ 80+4 g/ha, clodinafop propargyl @ 60 g/ha, clodinafop propargyl+2,4-D @ 60+600 g/ha and clodinafop propargyl+metsulfuron methyl @ 60+4 g/ha were tested with weed free and weedy check. Among the herbicides, application of clodinafop propargyl+ metsulfuron (60+4 g/ha) though statistically on par with clodinafop propargyl+ 2,4-D (60+600 g/ha) reduced the weed population significantly than weedy check (97.2%), clodinafop propargyl alone (88.0%), fenoxaprop -p-ethyl alone (83.0%), isoproturon alone (80.0%), isoproturon+metsulfuron (72.2%), pendimethalin (71.4%), isoproturon+2,4-D (53.8%), fenoxa prop -p-ethyl +2,4-D (53.8%) and fenoxa prop -p-ethyl + metsulfuron (40.0%) at 60 days after sowing stage. Among the herbicides, application of Clodinafop propargyl+ metsulfuron methyl (60+4 g/ha) as post emergence resulted into highest grain yield of wheat (49.28q/ha) which was on par with weed free and clodinafop propargyl+2,4-D @ 60+600 g/ha but statistically significant than rest of the treatments. Highest NPK uptake by grain and straw of wheat was recorded in weed free conditions compared to the herbicidal treatments, except lodinafop propargyl+metsulfuron methyl and clodinafop propargyl+ 2, 4-D. Highest removal of NPK (63.6 kg/ha) by weeds was recorded in weedy check whereas, the minimum NPK uptake (28.4 kg/ha) was noticed with the application of clodinafop propargyl+metsulfuron methyl due to efficient control of weeds (weed control efficiency 84.7%), which resulted in lowest weed dry matter in this treatments.

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Effect of NPK uptake at different growth stages of wheat (Triticum aestivum L.) for yield maximization

AN ASIAN JOURNAL OF SOIL SCIENCE ◽

10.15740/has/ajss/9.2/265-270 ◽

2014 ◽

Vol 9 (2) ◽

pp. 265-270

Author(s):

DIWAKAR PASWAN ◽

ANJANA ARUN ◽

AJEET KUMAR ◽

C.S. CHOUDHARY

Keyword(s):

Triticum Aestivum ◽

Triticum Aestivum L ◽

Growth Stages ◽

Npk Uptake ◽

Yield Maximization ◽

Different Growth Stages

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AKTIVITAS ENZIM PELARUT FOSFAT DAN EFEKTIVITAS MIKROBA ASAL WAMENA UNTUK MENUNJANG PERTANIAN RAMAH LINGKUNGAN PADA DAERAH MARGINAL

Jurnal Teknologi Lingkungan ◽

10.29122/jtl.v11i3.1194 ◽

2016 ◽

Vol 11 (3) ◽

pp. 481

Author(s):

Sri Widawati

Keyword(s):

Microbial Population ◽

Farming System ◽

Plate Count ◽

Phosphate Solubilizing Bacteria ◽

Clear Zone ◽

Environmental Friendliness ◽

Key Word ◽

Phosphate Dissolution ◽

Phosphate Solubilizing ◽

Organic Farming System

The activity of phosphate solubilizing enzyme and the effectivity of microbe from Wamena for support agriculture of environmental friendliness at marginal area. Phosphate dissolution of enzyme by microbial effective in compost plus from Wamena forest was stored in the freezer for 4 years, have been conducted in Ecofisiology laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute of Science. This research was conducted to know the microbial affectivity and the activity of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years in refrigerated room and to support soil fertility and biofertilizer agent in organic farming system. This research used plate count methode for counting the amount of microbial population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20microorganisms for 4 years in refrigerated room showed that the microbial activity still high with the amount of mean population 107 and the activity of phosphate solubilizing enzyme with mean diameter of clear zone (holozone) 1 cm – 2 cm. The effectivity and the highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), Bacillus panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosarium (L), and the lowest were: Streptomyces sp. (I) .Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer

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