scholarly journals AKTIVITAS ENZIM PELARUT FOSFAT DAN EFEKTIVITAS MIKROBA ASAL WAMENA UNTUK MENUNJANG PERTANIAN RAMAH LINGKUNGAN PADA DAERAH MARGINAL

2016 ◽  
Vol 11 (3) ◽  
pp. 481
Author(s):  
Sri Widawati
Keyword(s):  
Microbial Population ◽  
Farming System ◽  
Plate Count ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Environmental Friendliness ◽  
Key Word ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing ◽  
Organic Farming System

The activity of phosphate solubilizing enzyme and the effectivity of microbe from Wamena for support agriculture of environmental friendliness at marginal area. Phosphate dissolution of enzyme by microbial effective in compost plus from Wamena forest was stored in the freezer for 4 years, have been conducted in Ecofisiology laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute of Science. This research was conducted to know the microbial affectivity and the activity of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years in refrigerated room and to support soil fertility and biofertilizer agent in organic farming system. This research used plate count methode for counting the amount of microbial population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20microorganisms for 4 years in refrigerated room showed that the microbial activity still high with the amount of mean population 107 and the activity of phosphate solubilizing enzyme with mean diameter of clear zone (holozone) 1 cm – 2 cm. The effectivity and the highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), Bacillus panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosarium (L), and the lowest were: Streptomyces sp. (I) .Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer

scholarly journals Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut

2018 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Ella Dewani Larasati ◽  
MG Isworo Rukmi ◽  
Endang Kusdiyantini ◽  
R Cinta Badia Ginting
Keyword(s):  
Growth And Development ◽  
Bacillus Pumilus ◽  
Peat Soil ◽  
Species Level ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Peat Soils ◽  
Biolog System ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification

scholarly journals ISOLASI, POPULASI DAN KARAKTERISASI BAKTERI PELARUT FOSFAT PADA DAERAH PERAKARAN DAN TANAH DARI BENGKULU, SUMATRA

2016 ◽  
Vol 13 (1) ◽  
pp. 101
Author(s):  
Sri Purwaningsih
Keyword(s):  
Key Words ◽  
Pure Culture ◽  
Growth Characteristic ◽  
Plate Count ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Standard Medium ◽  
Phosphate Solubilizing

Penelitian dilakukan untuk mengetahui isolasi, populasi dan karakterisasi bakteri pelarut fosfat pada tanah dari rizosfer tanaman dari Bengkulu, Sumatra. Tujuan penelitian untuk mengetahui populasi bakteri pelarut fosfat dari rizosfer tanaman, dan mendapatkan kultur murni. Sampel diambil dari 25 sampel tanah dari rizosfer tanaman. Isolasi dilakukan dengan metode dilusi dalam Pycosvkaya menengah standar, inculation di (27-280C) setelah 15 hari, dan penduduk dengan metode plate count. Populasi bakteri pelarut fosfat berada di kisaran 13-101 105 X tanah CFU / g, dan populasi tertinggi ditemukan dari sampel tanah dari rhizoaphere dengan Semangka (Citrulus lanatus) tanaman. Tujuh puluh lima memberikan budaya murni. Karakteristik pertumbuhan kultur murni diamati dengan menggunakan media Pycosvkaya. Inkubasi di (27-280C) setelah 15 hari. Zona jelas mengelilingi koloni menunjukkan bahwa fosfat dalam medium adalah terhidrolisis dan zona terbentuk dari dua hari sampai delapan hari kata kunci: Bakteri pelarut Fosfat, Pycosvkaya, Bengkulu AbstractThe research was conducted in order to know the isolation, population and characterizations of phosphate solubilizing bacteria on the soil from rhizosphere plants from Bengkulu, Sumatra. The purpose of the study to know the population of phosphate solubilizing bacteria from rhizosphere plants, and the get pure culture. The sample wastaken from 25 sample soil from rhizosphere of plants. Isolation was done with dilution methods in standard medium Pycosvkaya, the inculation at (27-280C) after 15 days, and the population with plate count methods. The population of phosphate solubilizing bacteria was in the range of 13-101 X 105 CFU/g soil, and the highest population wasfound from the sample soil from rhizoaphere with Semangka (Citrulus lanatus) plant. Seventy five gave of pure culture. The growth characteristic of pure culture was observed by using Pycosvkaya medium. Incubation at (27-280C) after 15 days. The clear zone surround the colonies indicate that the phosphate in the medium was hydrolyzed and the formed zone from two days until eight days key words: Phosphate Solubilizing Bacteria, Pycosvkaya, Bengkulu 

scholarly journals Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome

2019 ◽  
Vol 20 (11) ◽  
Author(s):  
Dermiyati Dermiyati ◽  
Radix Suharjo ◽  
Mareli Telaumbanua ◽  
Yeyen Ilmiasari ◽  
Rully Yosita ◽  
...  
Keyword(s):  
Palm Oil ◽  
Microbial Population ◽  
Organic Fertilizer ◽  
Phosphate Solubilizing Bacteria ◽  
Anaerobic Conditions ◽  
Bacterial Colony ◽  
Facultative Anaerobic ◽  
Phosphate Solubilizing ◽  
Industrial Company ◽  
Very High

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Ilmiasari Y, Yosita R, Annisa RM, Sari AW, Andayani AP, Yulianti DM. 2019. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome. Biodiversitas 20: 3315-3321. Palm oil bunches and pineapple rhizomes are abundant in Indonesia, especially in Lampung Province due to widespread agro-industrial company and they are potential to be used as sources for liquid organic fertilizer. This study was aimed to reveal microbial population within liquid organic fertilizer (LF) which was developed from palm oil bunches (PB) and pineapple rhizome (PR) and their role as phosphate solubilizing bacteria (PSB). The capability to solubilize phosphate was recorded from the clear zone area around the bacterial colony which was cultivated on Pikovskaya’s agar medium. The LF was prepared in three kinds of conditions, namely aerobic, anaerobic, and facultative an aerobic. Isolation was performed every 3 days until 24 days after incubations. The results showed that microbial population in the LF from PR was significantly higher than those developed from PB. Totally, 791 bacterial isolates were obtained from the LF; 490 isolates were achieved from PR (153 isolates from aerobic, 188 isolates from facultative anaerobic, 149 from anaerobic conditions) and 301 isolates were collected from PB (96 isolates from aerobic, 112 isolates from facultative anaerobic, 93 isolates from anaerobic conditions). In the case of isolates that were gained from PB, 166 isolates (55.15%) showed capability to degrade phosphate and 135 isolates (44.85%) did not show any capability as PSB. As for isolates obtained from PR, 269 isolates (54.90%) had capability to solubilize phosphate, meanwhile, the other 221 isolates (45.10%) did not show any capability to solubilize phosphate. Among166 isolates of PSB from PB, 60 isolates had very low capability, 55 isolates had low capability, 34 isolates had medium capability, 10 isolates had high capability and only 7 isolates had very high capability. Meanwhile, from 269 isolates of PSB obtained from PR, 135 isolates had very low capability to solubilize phosphate, 84 isolates had low capability to solubilize phosphate, 32 had medium capability to solubilize phosphate, 14 isolates had high capability to solubilize phosphate and 4 isolates produced very high capability as PSB. In total, 11 chosen isolates were selected from LF developed from both PB (7 isolates) and PR (4 isolates). Therefore, palm oil bunches and pineapple rhizomes contained PSB that can be used as LF.

scholarly journals Isolation and selection of highly effective phosphate solubilizing bacterial strains to promote wheat growth in Egyptian calcareous soils

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Abdallah E. Mohamed ◽  
Maher Georg Nessim ◽  
Islam Ibrahim Abou-el-seoud ◽  
Khaled Mohamed Darwish ◽  
Abdelaal Shamseldin
Keyword(s):  
16S Rrna ◽  
Tricalcium Phosphate ◽  
Root Zone ◽  
Calcareous Soils ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Highly Efficient ◽  
Northwestern Coast ◽  
Phosphate Solubilizing

Abstract Background Forty phosphate solubilizing bacterial (PSB) isolates were isolated from the root zone of wheat plants cultivated in the Delta and the Northwestern coast regions of Egypt. All isolates proofed their ability to dissolve tricalcium phosphate on the National Botanical Research Institute's phosphate growth medium (NBRIP) by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l−1. They were designated as Egyptian native phosphate solubilizing bacteria (ENPSB). Results All strains proofed their ability to dissolve tricalcium phosphate on (NBRIP) medium by producing clear zone and increasing the available phosphorus that ranged between 40 and 707 mg l−1. The ENPSB 1, 2, and 3 strains were highly efficient as they gave 707, 653, and 693 mg l−1 soluble phosphorus respectively. Intriguingly, the two highly efficient strains for phosphate solubilization were isolated from the Northwestern coast alkaline soils. Moreover, 75% of strains were also produced profitable amounts of indole acetic acid (IAA) ranged from 0.79 to 50.5 mg l−1. Amazingly, the most efficient strain ENPSB 1 in solubilizing phosphorus (707 mg l−1soluble P) was the best one for producing IAA (50.5 mg l−1). The three efficient strains were identified using the sequencing of 16S rRNA. Sequence analysis of 16S rRNA for selected strains confirmed that the strains ENPSB 1, 2, and 3 were genetically closed to Enterobacter aerogenes; Pantoea sp. and Enteriobacter sp. respectively. Conclusion The inoculation by mix cultures of strains (ENPSB 1, 2, and 3) contributed to raising the dry weight and P content of wheat plants by 76% and 12% over the full fertilized plants. Inoculation of soil PSB can be used to solve the deficiency of phosphorus and promote plant growth effectively in calcareous soils.

scholarly journals Impacts of Organic Farming System on the Soil Microbial Population in Upland Soil

2011 ◽  
Vol 44 (5) ◽  
pp. 819-823 ◽  
Author(s):  
Young-Han Lee ◽  
Yeon-Kyu Sonn ◽  
Byung-Koo Ahn ◽  
Seong-Tae Lee ◽  
Min-A Shin ◽  
...  
Keyword(s):  
Organic Farming ◽  
Microbial Population ◽  
Farming System ◽  
Upland Soil ◽  
Soil Microbial ◽  
Organic Farming System ◽  
Soil Microbial Population

scholarly journals Study of Root Exudate Organic Acids and Microbial Population in the Rhizosphere of Oil Palm Seedling

2017 ◽  
Vol 22 (1) ◽  
pp. 29-36
Author(s):  
. Anandyawati ◽  
Enok Sumarsih ◽  
Budi Nugroho ◽  
Rahayu Widyastuti
Keyword(s):  
Organic Acids ◽  
Root Exudates ◽  
Oil Palm ◽  
Root Exudate ◽  
Microbial Population ◽  
Phosphate Solubilizing Bacteria ◽  
Randomized Design ◽  
Increase Productivity ◽  
Positive Correlations ◽  
Phosphate Solubilizing

Mutual interaction between plants and microbes occured in the rhizosphere is expected to increase productivity of crops or soil fertility for agriculture. Plants excrete root exudates to attract microbes, and then microbes obtain habitat and food supply from plants and can fulfill the nutrient requirements through assisted enzymatic activity.  The objective of the research was to study the types and amounts of root exudate organic acids, microbial population, and the relationship between root exudate organic acids and microbial population in the rhizosphere of oil palm seedlings. The study was conducted in a greenhouse using a planting medium of sterile quartz sand. The study was conducted using two factorials completely randomized design with three replications. The first factor was oil palm seedling age (control / no oil palm seed, 1, 3, 6, 9 and 12 months-old of oil palm seedlings) and the second factor was the periods of seedling growth (45, 90, 135 and 180 days), so in total there were 72 experimental units. The result of High Pressure Liquid Chromatography (HPLC) analysis revealed that four kinds of organic acids were observed in the rhizosphere of oil palm seedlings, with the highest concentration were: acetic acid (1.66 ppm), citric acid (0.157 ppm), malic acid (2.061 ppm) and oxalic acid (0.675) ppm. The highest total population of microbes, fungi, Azotobacter, phosphate solubilizing bacteria (PSB) and phosphate solubilizing fungi (PSF) were 19.38 × 106 cfu g-1 soil, 3.28 × 104 cfu g-1 soil, 12.09 × 105 cfu g-1 soil, 8.39 × 104 cfu g-1 soil and 1.15 × 104 cfu g-1 soil, respectively. There are positive correlations between root exudate organic acids and total microbes, fungi, Azotobacter, PSB and PSF are.Keywords: microbes, organic acids, rhizosphere, root exudates

scholarly journals Isolation, characterilzation and identification of phosphate solubilizing bacteria from rice-shrimp rotational farming system in salt affected soil areas of the mekong delta of Vietnam

2018 ◽  
Vol 15 (1) ◽  
pp. 121-131
Author(s):  
Nguyễn Khởi Nghĩa ◽  
Nguyễn Thị Kiều Oanh
Keyword(s):  
Bacterial Strain ◽  
Mekong Delta ◽  
Farming System ◽  
Salinity Range ◽  
Rrna Gene ◽  
Rice Soil ◽  
Phosphate Solubilizing Bacteria ◽  
Cell Numbers ◽  
Salt Affected Soil ◽  
Phosphate Solubilizing

Phosphorus is the least mobile element in plant and soil contrary to other macronutrients. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants. Aim of study was to deal with isolation, characterization and identification of phosphate solubilizing bacteria (PSBs) from 15 paddy rice soil samples collected in rice–shrimp rotational farming system in salt affected soil areas of the Mekong Delta of Vietnam including Bac Lieu, Ca Mau, Soc Trang, Kien Giang and Ben Tre by using NBRIP media containing 1% NaCl. Concentration of phosphate was determined by method of colometric determination of molybdate. Results showed that 95 isolates indicating potentially as PSBs through a exhibiting of a halo zone happening around bacterial colonies on NBRIP media. Twenty out of ninety five isolates showed their highly phosphate solubilizing ability (>1000 mg.L-1) in the liquid culture. Bacterial strain coded as BL1-10 solubilized maximumly 2044 mg.L-1 phosphate in liquid media after 5 incubation days. The cell number and phosphate solubilizing ability of Bl-10 strain were till good and not affected under environmental coditions like 40oC, salinity range between 0.5 and 5 % NaC and pH range between 3 and 5. Three kinds of trade name fungicides like DA roral, Topsin M và Antracol 70WP did not affect on cell numbers and phosphate solubilizing ability of Bl-10 strain. However, cell numbers and function of phosphate solubilizing ability of this bacterial strain was completely inhibited by Ridomil Gold fungicide. This strain was genetically identified as species of Burkholderia sp. BL1-10 based on results of the 16S rRNA gene sequence analysis. In conclusion, the study suggests the most promising phosphate solubilizing bacteria, Burkholderia sp. BL1-10 can be used as efficient biofertilizer inoculant to promote plant growth.

scholarly journals Effect of different phosphorus sources applied with phosphate solubilizing bacteria on bio-geochemical properties and phosphorus release pattern in vertisol

2021 ◽  
Vol 13 (2) ◽  
pp. 715-722
Author(s):  
D. Vignesh ◽  
P. Senthilvalavan ◽  
R. Manivannan ◽  
C. Ravikumar
Keyword(s):  
Soil Ph ◽  
Microbial Population ◽  
Phosphorus Release ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Release Pattern ◽  
Geochemical Properties ◽  
Phosphate Solubilizing ◽  
P Fertilizers ◽  
P Sources

Various phosphorus (P) fertilizers are used for crop production in different types of soil. But there is a knowledge gap in choosing the right source and form of  P fertilizers to enhance the applied fertilizer use efficiency. An experiment was taken to identify the best phosphorus source and its effectiveness as a source of P in vertisol to unravel this problem of selecting suitable P fertilizer. With this background, an incubation experiment was conducted under laboratory condition to determine the phosphorus release pattern of different P sources [Single Super Phosphate (SSP), Rock Phosphate (RP), Diammonium Phosphate (DAP), Nano phosphate (Nano P), Phosphocompost (PC)] applied with phosphate solubilizing bacteria (PSB) and their influence on biogeochemical properties in vertisol.  Experimental results emphasized that P release from different sources was influenced by soil pH, electrical conductivity (EC), cation exchange capacity (CEC), soil organic carbon (SOC), and microbial population. Applied P sources significantly(p=0.05) influenced the CEC, SOC, and microbial population except for soil pH and Ec. The maximum release in available P was obtained at 30 and 60 days after incubation with SSP +PSB  (35.8 and 40.1 mg kg-1) and Nano P + PSB (33.9 and 38.6 mg kg-1) applied treatments, respectively.Whereas at 90 days after incubation Nano P + PSB (42.3 mg kg-1) and Phosphocompost + PSB (40.4 mg kg-1) treatments recorded the maximum P availability and minimum P (15.2, 13.9 and 11.8 mg kg -1) release was noticed in the control treatment throughout the period of incubation. It was evident that SSP or Nano P along with PSB application might be the best P source for Vertisol.

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