scholarly journals Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome

2019 ◽  
Vol 20 (11) ◽  
Author(s):  
Dermiyati Dermiyati ◽  
Radix Suharjo ◽  
Mareli Telaumbanua ◽  
Yeyen Ilmiasari ◽  
Rully Yosita ◽  
...  
Keyword(s):  
Palm Oil ◽  
Microbial Population ◽  
Organic Fertilizer ◽  
Phosphate Solubilizing Bacteria ◽  
Anaerobic Conditions ◽  
Bacterial Colony ◽  
Facultative Anaerobic ◽  
Phosphate Solubilizing ◽  
Industrial Company ◽  
Very High

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Ilmiasari Y, Yosita R, Annisa RM, Sari AW, Andayani AP, Yulianti DM. 2019. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome. Biodiversitas 20: 3315-3321. Palm oil bunches and pineapple rhizomes are abundant in Indonesia, especially in Lampung Province due to widespread agro-industrial company and they are potential to be used as sources for liquid organic fertilizer. This study was aimed to reveal microbial population within liquid organic fertilizer (LF) which was developed from palm oil bunches (PB) and pineapple rhizome (PR) and their role as phosphate solubilizing bacteria (PSB). The capability to solubilize phosphate was recorded from the clear zone area around the bacterial colony which was cultivated on Pikovskaya’s agar medium. The LF was prepared in three kinds of conditions, namely aerobic, anaerobic, and facultative an aerobic. Isolation was performed every 3 days until 24 days after incubations. The results showed that microbial population in the LF from PR was significantly higher than those developed from PB. Totally, 791 bacterial isolates were obtained from the LF; 490 isolates were achieved from PR (153 isolates from aerobic, 188 isolates from facultative anaerobic, 149 from anaerobic conditions) and 301 isolates were collected from PB (96 isolates from aerobic, 112 isolates from facultative anaerobic, 93 isolates from anaerobic conditions). In the case of isolates that were gained from PB, 166 isolates (55.15%) showed capability to degrade phosphate and 135 isolates (44.85%) did not show any capability as PSB. As for isolates obtained from PR, 269 isolates (54.90%) had capability to solubilize phosphate, meanwhile, the other 221 isolates (45.10%) did not show any capability to solubilize phosphate. Among166 isolates of PSB from PB, 60 isolates had very low capability, 55 isolates had low capability, 34 isolates had medium capability, 10 isolates had high capability and only 7 isolates had very high capability. Meanwhile, from 269 isolates of PSB obtained from PR, 135 isolates had very low capability to solubilize phosphate, 84 isolates had low capability to solubilize phosphate, 32 had medium capability to solubilize phosphate, 14 isolates had high capability to solubilize phosphate and 4 isolates produced very high capability as PSB. In total, 11 chosen isolates were selected from LF developed from both PB (7 isolates) and PR (4 isolates). Therefore, palm oil bunches and pineapple rhizomes contained PSB that can be used as LF.

scholarly journals AKTIVITAS ENZIM PELARUT FOSFAT DAN EFEKTIVITAS MIKROBA ASAL WAMENA UNTUK MENUNJANG PERTANIAN RAMAH LINGKUNGAN PADA DAERAH MARGINAL

2016 ◽  
Vol 11 (3) ◽  
pp. 481
Author(s):  
Sri Widawati
Keyword(s):  
Microbial Population ◽  
Farming System ◽  
Plate Count ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Environmental Friendliness ◽  
Key Word ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing ◽  
Organic Farming System

The activity of phosphate solubilizing enzyme and the effectivity of microbe from Wamena for support agriculture of environmental friendliness at marginal area. Phosphate dissolution of enzyme by microbial effective in compost plus from Wamena forest was stored in the freezer for 4 years, have been conducted in Ecofisiology laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute of Science. This research was conducted to know the microbial affectivity and the activity of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years in refrigerated room and to support soil fertility and biofertilizer agent in organic farming system. This research used plate count methode for counting the amount of microbial population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20microorganisms for 4 years in refrigerated room showed that the microbial activity still high with the amount of mean population 107 and the activity of phosphate solubilizing enzyme with mean diameter of clear zone (holozone) 1 cm – 2 cm. The effectivity and the highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), Bacillus panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosarium (L), and the lowest were: Streptomyces sp. (I) .Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer

scholarly journals Vermicomposting: An Effective Option for Recycling Organic Wastes

2020 ◽  
Author(s):  
Tamanreet Kaur
Keyword(s):  
Low Cost ◽  
Organic Fertilizer ◽  
Organic Wastes ◽  
Growth Promoter ◽  
Phosphate Solubilizing Bacteria ◽  
Suitable Alternative ◽  
Effective Option ◽  
Phosphate Solubilizing ◽  
Water And Soil Pollution

Urbanization and industrialization resulted in rapid increase in volume of solid waste; its management has become one of the biggest problems today. Solid wastes can be disposed off by methods like land filling, incineration, conversion into biogas, recycling, and composting, but its overproduction has led to inappropriate disposal practices such as their indiscriminate and inappropriately timed application to agricultural fields that ultimately leads to water and soil pollution. However, if handled properly, these organic wastes can be used for vermicomposting; it is an effective recycling technology that improves the quality of the products which is disinfected, detoxified, and highly nutritive. It is a low cost, eco-biotechnological process of waste management in which earthworms are used to cooperate with microorganisms in order to convert biodegradable wastes into organic fertilizer. Earthworms excreta (vermicast) is a nutritive organic fertilizer rich in humus, NPK, micronutrients, beneficial soil microbes; nitrogen-fixing, phosphate solubilizing bacteria, actinomycets, and growth hormones auxins, gibberlins and cytokinins, is a suitable alternative to chemical fertilizers, being an excellent growth promoter and protector for crop plants. Thus, vermiculture not only results in management of soild waste but also produces excellent nutrient enriched vermicompost. Vermicompost is beneficial for sustainable organic agriculture and maintaining balanced ecosystem.

scholarly journals Study of Root Exudate Organic Acids and Microbial Population in the Rhizosphere of Oil Palm Seedling

2017 ◽  
Vol 22 (1) ◽  
pp. 29-36
Author(s):  
. Anandyawati ◽  
Enok Sumarsih ◽  
Budi Nugroho ◽  
Rahayu Widyastuti
Keyword(s):  
Organic Acids ◽  
Root Exudates ◽  
Oil Palm ◽  
Root Exudate ◽  
Microbial Population ◽  
Phosphate Solubilizing Bacteria ◽  
Randomized Design ◽  
Increase Productivity ◽  
Positive Correlations ◽  
Phosphate Solubilizing

Mutual interaction between plants and microbes occured in the rhizosphere is expected to increase productivity of crops or soil fertility for agriculture. Plants excrete root exudates to attract microbes, and then microbes obtain habitat and food supply from plants and can fulfill the nutrient requirements through assisted enzymatic activity.  The objective of the research was to study the types and amounts of root exudate organic acids, microbial population, and the relationship between root exudate organic acids and microbial population in the rhizosphere of oil palm seedlings. The study was conducted in a greenhouse using a planting medium of sterile quartz sand. The study was conducted using two factorials completely randomized design with three replications. The first factor was oil palm seedling age (control / no oil palm seed, 1, 3, 6, 9 and 12 months-old of oil palm seedlings) and the second factor was the periods of seedling growth (45, 90, 135 and 180 days), so in total there were 72 experimental units. The result of High Pressure Liquid Chromatography (HPLC) analysis revealed that four kinds of organic acids were observed in the rhizosphere of oil palm seedlings, with the highest concentration were: acetic acid (1.66 ppm), citric acid (0.157 ppm), malic acid (2.061 ppm) and oxalic acid (0.675) ppm. The highest total population of microbes, fungi, Azotobacter, phosphate solubilizing bacteria (PSB) and phosphate solubilizing fungi (PSF) were 19.38 × 106 cfu g-1 soil, 3.28 × 104 cfu g-1 soil, 12.09 × 105 cfu g-1 soil, 8.39 × 104 cfu g-1 soil and 1.15 × 104 cfu g-1 soil, respectively. There are positive correlations between root exudate organic acids and total microbes, fungi, Azotobacter, PSB and PSF are.Keywords: microbes, organic acids, rhizosphere, root exudates

scholarly journals Isolation of phosphate-solubilizing bacteria from subtropical soils with different fertilization histories

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Juliana Maria Matter ◽  
Silvio César Sampaio ◽  
Danielle Medina Rosa ◽  
Marcelo Bevilacqua Remor ◽  
Kathleen Jeniffer Model ◽  
...  
Keyword(s):  
Chemical Elements ◽  
Organic Fertilizer ◽  
Negative Relationship ◽  
Mineral Fertilizer ◽  
Swine Wastewater ◽  
Phosphate Solubilizing Bacteria ◽  
Colony Forming Units ◽  
Phosphate Solubilizing ◽  
Bioavailability Index ◽  
Inorganic Phosphates

Phosphorus is one of the most abundant chemical elements but has a low bioavailability index. Therefore, microrganisms play a fundamental role in providing insoluble phosphorus to plants. The objective of this study was to evaluate the capacity of bacteria to solubilize inorganic phosphates in soils with different fertilization histories. Soil and rhizosphere samples were collected from a Red Distroferric Latosol, including a control without mineral or organic fertilizer (C), treatment with mineral fertilizer (MF) according to the needs of each crop, and treatment with organic fertilizer [300 m3 ha-1 of swine wastewater (SW)]. The medium containing calcium phytate presented more colony-forming units (CFU) for all fertilization histories, and growth in treatments C and MF was 50% higher than treatment with SW. CFU values in soils treated with SW were lower than those in the other treatments, and the diversity of insoluble phosphate-solubilizing bacteria (PSB) was higher in treatment C. These results indicate a negative relationship between phosphorus concentrations and the number of PSB.

scholarly journals Effect of different phosphorus sources applied with phosphate solubilizing bacteria on bio-geochemical properties and phosphorus release pattern in vertisol

2021 ◽  
Vol 13 (2) ◽  
pp. 715-722
Author(s):  
D. Vignesh ◽  
P. Senthilvalavan ◽  
R. Manivannan ◽  
C. Ravikumar
Keyword(s):  
Soil Ph ◽  
Microbial Population ◽  
Phosphorus Release ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Release Pattern ◽  
Geochemical Properties ◽  
Phosphate Solubilizing ◽  
P Fertilizers ◽  
P Sources

Various phosphorus (P) fertilizers are used for crop production in different types of soil. But there is a knowledge gap in choosing the right source and form of  P fertilizers to enhance the applied fertilizer use efficiency. An experiment was taken to identify the best phosphorus source and its effectiveness as a source of P in vertisol to unravel this problem of selecting suitable P fertilizer. With this background, an incubation experiment was conducted under laboratory condition to determine the phosphorus release pattern of different P sources [Single Super Phosphate (SSP), Rock Phosphate (RP), Diammonium Phosphate (DAP), Nano phosphate (Nano P), Phosphocompost (PC)] applied with phosphate solubilizing bacteria (PSB) and their influence on biogeochemical properties in vertisol.  Experimental results emphasized that P release from different sources was influenced by soil pH, electrical conductivity (EC), cation exchange capacity (CEC), soil organic carbon (SOC), and microbial population. Applied P sources significantly(p=0.05) influenced the CEC, SOC, and microbial population except for soil pH and Ec. The maximum release in available P was obtained at 30 and 60 days after incubation with SSP +PSB  (35.8 and 40.1 mg kg-1) and Nano P + PSB (33.9 and 38.6 mg kg-1) applied treatments, respectively.Whereas at 90 days after incubation Nano P + PSB (42.3 mg kg-1) and Phosphocompost + PSB (40.4 mg kg-1) treatments recorded the maximum P availability and minimum P (15.2, 13.9 and 11.8 mg kg -1) release was noticed in the control treatment throughout the period of incubation. It was evident that SSP or Nano P along with PSB application might be the best P source for Vertisol.

scholarly journals Sustainable Agronomic Valorization of Unsulfured Molasses and Defatted Soybean Meal as an Optimized Formulation of Bio-Organic Fertilizer Enriched with High Cell Density P-Solubilizing Bacteria

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 996
Author(s):  
Muhamad Aidilfitri Mohamad Roslan ◽  
Izzalan Sohedein ◽  
Puan Sheau Ling ◽  
Zulfazli M. Sobri ◽  
Ali Tan Kee Zuan ◽  
...  
Keyword(s):  
Cell Density ◽  
Soybean Meal ◽  
Large Scale ◽  
Organic Fertilizer ◽  
Viable Cell ◽  
Viable Cell Density ◽  
Phosphate Solubilizing Bacteria ◽  
Maximum Cell Density ◽  
Defatted Soybean Meal ◽  
Phosphate Solubilizing

The application of plant beneficial bioinoculants such as phosphate solubilizing bacteria is a sustainable approach to expanding crop performance in agriculture. However, bioinoculant strains, particularly non-sporulating bacteria are often exposed to detrimental conditions throughout the production process and a long period of storage. This will negatively influence their viable cell density and eventually limit its efficacy in the field. To overcome such a scenario, an optimal formulation of biofertilizer should be prioritized. In this report, a sustainable valorization of molasses and defatted soybean meal as formulation of biofertilizer enriched with Enterobacter hormaechei 40a was proposed. Through the two-level factorial design and central composite design, the optimal formulation and fermentation conditions of bio-organic fertilizer to achieve maximum cell density of strain 40a were achieved. The highest cell density of strain 40a in the optimized molasses-DSM (OMD) medium was 12.56 log CFU/mL after 24 h which was 99.7% accuracy towards the predicted value. Interestingly, the solubilized P was increased by 62.4% in the OMD medium (174.07 µg/mL P) as compared to the standard P medium (65.38 µg/mL P). The shelf life of strain 40a after 180 days of storage was improved significantly around 10 log CFU/mL, when the OMD medium was amended with 0.1% sodium alginate. The strategy described here offers opportunities for agronomic formulation and large-scale bio-organic fertilizer production in the agriculture industry.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

ANALYSIS FOR POTENCY AND PRODUCTION OF GRANULE ORGANIC FERTILIZER BASED ON MANURE

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Firman L. Sahwan
Keyword(s):  
Organic Material ◽  
Organic Materials ◽  
Raw Materials ◽  
Organic Fertilizer ◽  
Raw Material ◽  
Cow Manure ◽  
Production Potential ◽  
Natural Organic Material ◽  
Very High

Organic materials that are generally used as raw material for organic fertilizer granules (POG) is a natural organic material that has been degrade, smooth and dry. One of the main raw materials are always used with a very high percentage of usage, is manure. Manure potential in Indonesia is very high, amounting to 113.6 million tons per year, or 64.7 million tons per year to the island of Java. From this amount, it will be generated numbers POG production potential of 17.5 million tons per year (total Indonesia) or 9.9 million tons per year for the island of Java. While the realistic POG production predictions figures made from raw manure is 2.5 million tons annually, a figure that has been unable to meet the number requirement of POG greater than 4 million tons per year. Therefore, in producing POG, it should be to maximize the using of the potential of other organic materials so that the use of manure can be saved. With the use of a small amount of manure (maximum 30% for cow manure), it would be useful also to avoid the production of POG with high Fe content.keywods: organic material, manure, granule organic fertilizer

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