scholarly journals Effect of Bio-Pesticides on Mortality of Greater Wax Moth Galleria mellonella L.

2021 ◽  
pp. 39-47
Author(s):  
S. Balpande ◽  
A. S. Yadav
Keyword(s):  
Bacillus Thuringiensis ◽  
Galleria Mellonella ◽  
Larval Mortality ◽  
Storage Condition ◽  
Seed Extract ◽  
Neem Oil ◽  
Apple Seed ◽  
Bacillus Thuringiensis Kurstaki ◽  
Custard Apple ◽  
Percent Concentration

Galleria mellonella larvae feed on wax, pollen, honey and damage the combs with in active bee colonies and storage condition. Galleria mellonella larvae can bore tunnel and feed around the midrib base of the wax comb. Larvae produce silk fibers that can trap bee brood cells. Comb is completely covered with webbing and extracted matter of the larvae condition described as “Galleriasis”.  The study was carried out at Apiculture Lab of RVSKVV-ZARS-Krishi Vigyan Kendra, Morena (M.P.) during 2019-20. Different three concentration of bio-pesticides viz., Bacillus thuringiensis Kurstaki  (1, 1.5 and 2%), custard apple seed extract (2, 4and 6%) and Neem Oil (1, 2 and 3%) were used against the Galleria mellonella to assess the effect of larval mortality at 24, 48, 72 and 96 hours after treatment. There was no significant effect showed in all the treatments after 24 hours. The maximum mortality of Galleria mellonella larvae was recorded in Bacillus thuringiensis Kurstaki 2.0 percent concentration (46.67, 87.08 and 96.67%) after 48, 72 and 96 hours respectively and it was at par with custard apple seed extract 6.0 percent concentration (45.83, 85.0 and 93.75%). Neem oil 3.0 percent concentration expressed 38.33, 75.42 and 87.50 percent larval mortality after 48, 72 and 96 hours respectively. Whereas minimum mortality was noticed in Neem oil 1.0 percent at 48 hours (22.08%), at 72 hours (37.08%) and at 96 hours (56.25%) among the treatments. The higher concentration of all the three bio-pesticides was proved effectiveness against Galleria mellonella during investigation.

scholarly journals Effects of Neem (Azadirachta indic) and Custard Apple (Annona reticulata) Diets on Sterility of House Rat (Rattus rattus)

2016 ◽  
Vol 1 ◽  
pp. 37-40
Author(s):  
Prem Nidhi Sharma ◽  
Poorna Dass Dhaubaji Shrestha ◽  
Shiv Raj Chauhan ◽  
Vinita Sharma
Keyword(s):  
Single Dose ◽  
Rattus Rattus ◽  
Normal Diet ◽  
Control Group ◽  
Mixed Diet ◽  
Neem Seed ◽  
Neem Oil ◽  
Apple Seed ◽  
Custard Apple ◽  
Annona Reticulata

Three different plant products diets – i) neem (Azadirachta indic A. Juss) oil mixed diet (neem oil mixed @ 80 ml/kg of normal diet), ii) neem seed powder mixed diet (neem seed powder mixed @ 80 g/kg of normal diet) and iii) custard apple (Annona reticulata L.) seed powder mixed diet (custard apple seed powder mixed @ 80 g/kg of normal diet) were separately fed to mature rats (Rattus rattus) with single dose feeding of 80 g per pair in a day on 13th week-age during the experimenting years, 2012/013 and 2013/014. In control group only normal diet without neem and custard apple constituents were fed. Sterility test of rat was conducted up to 38 and 28 weeks-age in first and second year, respectively. The test rats were fed normal diet during whole experimenting periods except the one day when they were fed only the neem or custard apple mixed diet on the age of 13th week. Efficacy of the mixed diets on rat-sterility was determined based on pregnancy and parturition by the rats. The two years' results confirmed that all the tested three mixed diets – neem oil mixed diet, neem seed powder mixed diet, and custard apple seed powder mixed diet were effective to stop pregnancy and parturition in rats during whole experimenting periods up to 38 and 28 weeks-age with single dose feeding of 80 g per pair (40 gm/rat) in a day on 13th week-age of the rats; whereas the pregnancy and parturition were observed in the rats that were fed only the normal diet. It is expected, neem and custard apple mixed diets can be utilized in reducing the economically important rodent populations in rice-wheat cropping system in future.Journal of Nepal Agricultural Research Council Vol.1 2015, pp.37-40

scholarly journals Deterrent effect of plant extracts against Callosobruchus maculatus on stored cowpea in Saurashtra (Gujarat, India)

2015 ◽  
Vol 7 (1) ◽  
pp. 187-191 ◽  
Author(s):  
J. A. Chudasama ◽  
N. B. Sagarka ◽  
Satyakumari Sharma
Keyword(s):  
Dose Level ◽  
Plant Extracts ◽  
Leaf Extract ◽  
Callosobruchus Maculatus ◽  
Seed Extract ◽  
Deterrent Effect ◽  
Seed Kernel ◽  
Neem Seed ◽  
Apple Seed ◽  
Custard Apple

A laboratory study was conducted at the JAU, Junagadh (Gujarat) during 2013-14 on the deterrent effect of different aqueous plant extracts against pulse beetle, Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae) in stored seeds of cowpea, Vigna unguiculata (L.). Among different aqueous extracts of plants materials, maximum percentage of oviposition deterrence was observed in custard apple seed extract (67.19%), custard apple leaf extract (65.95%), neem seed extract (65.44%), mustard seed extract (64.65%) and jatropha leaf extract (64.16%) at 5 per cent concentration. The maximum adult emergence reduction was seen in custard apple seed extract (78.45%), custard apple leaf extract (77.14%) and neem seed kernel extract (NSKE) (77.09%) at 5 per cent dose level. Minimum weight loss percentage was observed in the cowpea seeds treated with custard apple seed extract at 5% dose level, which recorded 79.20%. The results revealed that all of the tested materials with some variations had deterrent and toxic effects against the pest.

scholarly journals Productivity, pathogenicity, host range, and spore mass-propagation of local strain of Mattesia sp. isolated from insect cadavers of certain stored grain pests in Egypt

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Ahlam Ahmed Alfazairy ◽  
Yasien Mohamed Gamal Zedan El-Abed ◽  
Hanan Mohamed Ramadan ◽  
Hedaya Hamza Karam
Keyword(s):  
Galleria Mellonella ◽  
Larval Mortality ◽  
Maximum Yield ◽  
Local Strain ◽  
Rhyzopertha Dominica ◽  
Stored Grain ◽  
Spore Yield ◽  
Long Term Storage ◽  
Spore Mass

AbstractAverage yields of Mattesia spores (spore productivity) had varied from a minimum yield (0.17 × 107 spores) for Laemophloeus turcicus adult to a maximum yield (7.46 × 107 spores) for Plodia interpunctella larva. Comparatively, the highest increase in Mattesia spore yield, recorded from P. interpunctella larva (7.46 × 107 spores) over the lowest one, estimated for L. turcicus adult (0.17 × 107 spores), was nearly 44-fold. The increase in Mattesia spore yields that calculated from the other hosts (P. interpunctella pupa or moth; Galleria mellonella larva; Rhyzopertha dominica adult; Sitophilus zeamais), over that estimated for L. turcicus adult, was less than 10-fold (6–9-fold). Based on the weight of 1 g of the insect host infected with Mattesia sp., small stored grain insect hosts (e.g. L. turcicus, S. zeamais, and R. dominica) seemed to achieve Mattesia spore yields more than the larger ones (e.g. P. interpunctella). The increase in spore yields over that used for the inoculum, based on an average of 25 P. interpunctella larvae per bioassay container, was ca. 2 to 31-fold. These results revealed that the Indianmeal moth, P. interpunctella, could serve as a potential host for mass propagating the isolated entomopathogenic protozoan, Mattesia sp. Besides Mattesia larval mortality, survivors of Mattesia infection suffered deformities and noticeable undersized pupae or adults than the control ones. Also, many copulated moths (ca.46%) were unable to become separated after copulation until they had died. Bioassay of siftings, obtained from L. turcicus-protozoan-infected stock cultures, was carried out in order to emphasize the suppressive potent role of such protozoan entomopathogens in long-term storage. With the highest tested concentration of the studied siftings (10%), mortality responses due to Mattesia infection ranged from 13 to 68% at 14–169 days post-treatment. The corresponding figures for Adelina infection were 7–42%.

LONG TERM STUDY OF THE EFFECTIVENESS OF AERIAL APPLICATION OF BACILLUS THURINGIENSIS – ACEPHATE COMBINATIONS AGAINST THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1977 ◽  
Vol 109 (9) ◽  
pp. 1239-1248 ◽  
Author(s):  
O. N. Morris
Keyword(s):  
Bacillus Thuringiensis ◽  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Abies Balsamea ◽  
Spruce Budworm ◽  
White Spruce ◽  
Balsam Fir ◽  
Population Densities ◽  
Long Term Study

AbstractBacillus thuringiensis (Dipel® 36B) mixed with a sublethal concentration of acephate (Orthene®) (O, S-dimethyl acetylphosphoramidothioate), an organophosphorous insecticide, was applied at 2.35–14 l./ha to white spruce (Picea glauca) and balsam fir (Abies balsamea) trees infested with spruce budworm, Choristoneura fumiferana (Clem.). The treatment rate was 20 Billion International Units of B. thuringiensis (B.t.) activity with or without 42 g of active ingredient of acephate/ha.The ground deposit of the standard Dipel wettable powder formulation was 12% of emitted volume compared with 21–32% for the Dipel 36B flowable. The viability of B.t. spores was drastically reduced after 1 day of weathering but a high level of biological activity by the spore–crystal complex persisted for up to 20 days post-spray due probably to crystal activity.The addition of about 10% of the recommended operational rate of acephate to the B.t. suspension increased larval mortality by 34% when applied at 4.7 l./ha. Reductions in budworm populations were 97–99% in B.t. + acephate plots and 86–90% in B.t. alone plots.Plots with moderate budworm densities of up to 27 larvae/100 buds on white spruce and 36/100 on balsam fir were satisfactorily protected from excessive defoliation in the year of spray by B.t. with or without acephate. Plots with higher population densities were not satisfactorily protected based on the branch sample examination but aerial color photographs indicated good protection to the top third of the trees. Population declines were greater and defoliation and oviposition were lower in the treated plots than in the untreated checks 1 year later without further treatment. Two years later the larval population densities in all plots were low but the density was twice as high in the untreated check as in the treated plots, indicating long term suppression by the treatments. Defoliation was negligible in all plots.The treatments had no deleterious effect on spruce budworm parasitism. The data indicate that the integrated approach using Bacillus thuringiensis – chemical pesticide combinations is a viable alternative to the use of chemical pesticides alone in spruce budworm control. Large scale testing is now warranted.

scholarly journals Effect of aqueous neem seed extract via irrigation on larvae of Liriomyza sativae in melon crop

2018 ◽  
Vol 36 (3) ◽  
pp. 353-356 ◽  
Author(s):  
Ewerton M Costa ◽  
Francisco EL Silva ◽  
Elton L Araujo
Keyword(s):  
Larval Mortality ◽  
Total Mortality ◽  
Seed Extract ◽  
Neem Seed ◽  
Liriomyza Sativae ◽  
Randomized Design ◽  
Soil Irrigation ◽  
Completely Randomized Design ◽  
Pupal Mortality ◽  
Neem Seed Extract

ABSTRACT The leafminer [Liriomyza sativae (Diptera: Agromyzidae)] is one of the main pests of melon crop (Cucumis melo) in the states of Rio Grande do Norte and Ceará. Thus, adopting strategies to control this destructive insect is essential. The aim of this study was to evaluate the effect of aqueous neem seed extract, applied to soil via irrigation, on leafminer larvae, in the melon crop. An experiment was conducted in greenhouse, in completely randomized design, consisting of five treatments (four doses of aqueous neem seed extract: 1; 3; 5 and 10 g/100 mL) and the control consisting only of distilled water and 10 replicates (melon plants). The treatments were applied in a single dose, via soil irrigation. In the first stage of the evaluation, 24 hours after application, the number of dead larvae per plant was counted. In the second step, pupal mortality was recorded. The total mortality was also calculated considering the number of larvae and the number of emerged adults. We verified that, increasing the concentration of aqueous neem seed extract, an increase in the larval and pupal mortality of the leafminer in melon crop was noticed. The concentration 10 g/100 mL presented the best results, resulting in 36.4% of larval mortality, 96.5% of pupal mortality and 96.8% of total mortality.

Sign in / Sign up

Export Citation Format

Share Document