scholarly journals First Report in Sudan: Detection of Antibacterial Activity of the Black Cumin (Nigella sativa) Seed Extract against Mycoplasma mycoides subsp mycoides (Mmm)

2019 ◽  
pp. 1-6
Author(s):  
Raghad A. H. Onsa ◽  
E. A. Muna ◽  
Samia A. A. Hassan
Keyword(s):  
Seed Oil ◽  
Nigella Sativa ◽  
Local Strain ◽  
Bactericidal Effect ◽  
Black Cumin ◽  
Veterinary Research ◽  
Mycoplasma Mycoides ◽  
Inhibition Zones ◽  
Nigella Sativa Seed

Aims: This study was focused on the effect of Nigella sativa seed oil on the in vitro growth of Mycoplasma mycoides subsp mycoides (Mmm). Study Design: Three strains of (Mmm) were subjected to different dilutions of extracted Nigella sativa seed oil and the inhibitory zones were recorded. Type of effect (bactericidal or bacteriostatic) was studied. Place and Duration of Study: The study was carried out at Mycoplasma and biochemistry departments Central Veterinary Research Laboratory (CVRL) - 2018. Methodology: The tested strains were: Reference strain (T1/44), recent local strain (RH strain) and the last one was an old local strain (F strain). Different dilutions were used from N. sativa (25%, 50%, 75% and 100%) respectively. Results: The N. sativa revealed different inhibition zones on the growth of the three mentioned cultures. In addition to that bactericidal effect on (Mmm) growth was observed. Conclusion: The result of this study suggests the potential use of Cumin seed (N. sativa) against (Mmm) in vitro, and this result contributes in CBPP disease control using this type of natural seeds.

scholarly journals A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil

2015 ◽  
Vol 39 ◽  
pp. 801-812 ◽  
Author(s):  
Didem AĞ ŞELECİ ◽  
Zinar Pınar GÜMÜŞ ◽  
Murat YAVUZ ◽  
Muharrem ŞELECİ ◽  
Rebecca BONGARTZ ◽  
...  
Keyword(s):  
Wheat Germ ◽  
Seed Oil ◽  
Biological Activities ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed ◽  
Black Cumin Seed Oil

scholarly journals Inhibitory effects of Nigella sativa seed oil on the testosterone-induced benign prostatic hyperplasia in rats

BioMedicine ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 19-15
Author(s):  
Arezo Sadeghimanesh ◽  
Sajedeh Gholipour ◽  
akram torki ◽  
hossein Amini-khoei ◽  
zahra lorigooini ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Seed Oil ◽  
Nigella Sativa ◽  
Prostatic Hyperplasia ◽  
Inhibitory Effects ◽  
Nigella Sativa Seed

scholarly journals In Vitro Gynogenesis and Flow Cytometry Analysis of the Regenerated Haploids of Black Cumin (Nigella sativa)

HortScience ◽  
2018 ◽  
Vol 53 (5) ◽  
pp. 681-686 ◽  
Author(s):  
Mohammed Elsayed El-Mahrouk ◽  
Mossad K. Maamoun ◽  
Antar Nasr EL-Banna ◽  
Soliman A. Omran ◽  
Yaser Hassan Dewir ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nigella Sativa ◽  
Plant Production ◽  
Haploid Plant ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Flow Cytometry Analysis ◽  
Black Cumin ◽  
Haploid Plants

In vitro ovule culture could be used to generate homozygous lines through the production of haploid plants. The present study reports on in vitro regeneration and production of haploid plants through ovule cultures and identification of the regenerated haploids using flow cytometry. The ovules were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine (BA), kinetin (Kin), 2,4-dichlorophenoxyacetic acid (2,4-D), and naphthalene acetic acid (NAA) at 0, 0.5, 1, and 2 mg·L−1 for their gynogenesis. Among different plant growth regulators (PGRs) tested, 2,4-D at 2 mg·L−1 produced direct gynogenesis. The highest callogenesis percentage (100%) was obtained on MS medium containing 1 mg·L−1 2,4-D and 2 mg·L−1 NAA. Flow cytometry analysis was used to identify the regenerated haploids. It also confirmed gynogenic occurrence at 1 and 2 mg·L−1 2,4-D with percentages of 21.7% and 41%, respectively. Therefore, 2,4-D proved effective for the induction of haploids in black cumin. The regenerated haploids were developed on MS medium without PGRs. The obtained results of in vitro gynogenesis and haploid plant production can tremendously facilitate breeding programs of black cumin.

scholarly journals Antioxidant and Anti-Inflammatory Properties of Nigella sativa Oil in Human Pre-Adipocytes

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 51 ◽  
Author(s):  
Laura Bordoni ◽  
Donatella Fedeli ◽  
Cinzia Nasuti ◽  
Filippo Maggi ◽  
Fabrizio Papa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Mediterranean Area ◽  
Low Grade ◽  
Mean Values ◽  
Black Cumin ◽  
Marche Region ◽  
Anti Inflammatory

The oil obtained from the seeds of Nigella sativa L. (N. sativa), also known as black cumin, is frequently used in the Mediterranean area for its anti-inflammatory, anti-oxidant, and anti-cancer activities. The aim of the present study was to evaluate the antioxidant and anti-inflammatory properties of the oil extracted from seeds of a N. sativa cultivar produced in the Marche region of Italy, and to determine if the thymoquinone content, antioxidant properties, and biological activity would decay during storage. Cytotoxicity and anti-inflammatory properties of N. sativa oil were tested in an in vitro model of low-grade inflammation in Simpson–Golabi–Behmel syndrome human pre-adipocytes. The fresh extracted oil (FEO) contained 33% more thymoquinone than stored extracted oil (SEO), demonstrating that storage affects its overall quality. In addition, the thymoquinone content in the N. sativa oil from the Marche region cultivar was higher compared with other N. sativa oils produced in the Middle East and in other Mediterranean regions. Pro-inflammatory cytokines (e.g., Interleukin (IL)-1alpha, IL-1beta, IL-6) were differently modulated by fresh and stored extracts from N. sativa oils: FEO, containing more thymoquinone reduced IL-6 levels significantly, while SEO inhibited IL-1beta and had a higher antioxidant activity. Total antioxidant activity, reported as µM of Trolox, was 11.273 ± 0.935 and 6.103 ± 0.446 for SEO and FEO (p = 1.255 × 10−7), respectively, while mean values of 9.895 ± 0.817 (SEO) and 4.727 ± 0.324 (FEO) were obtained with the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (p = 2.891 × 10−14). In conclusion, the oil capacity to counteract proinflammatory cytokine production does not strictly depend on the thymoquinone content, but also on other antioxidant components of the oil.

The sterols of Nigella sativa seed oil

1986 ◽  
Vol 25 (3) ◽  
pp. 761-763 ◽  
Author(s):  
Panagiotis Menounos ◽  
Kostis Staphylakis ◽  
Dina Gegiou
Keyword(s):  
Seed Oil ◽  
Nigella Sativa ◽  
Nigella Sativa Seed
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