scholarly journals Frequency of Different Bacterial Species and their Antibiogram among Patients with Chronic Otitis Media

2021 ◽  
pp. 215-220
Author(s):  
Mohammad Farooq Bhutta ◽  
Ashfaq Hussain ◽  
Salman Baig ◽  
Asad Ullah ◽  
Sarwath Fatimee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Antimicrobial Susceptibility ◽  
Proteus Mirabilis ◽  
Bacterial Species ◽  
Chronic Otitis Media ◽  
The Other ◽  
Suppurative Otitis Media

Aim: To find out the frequency of different bacterial species and their antibiogram among the patients of chronic suppurative otitis media. Study Design: Descriptivecross-sectional. Place and Duration of Study: The study was conducted at the ENT department of Bahawal Victoria hospital between July to December 2020. Methodology: About 50 patients presented with unilateral or bilateral ear discharge and diagnosed as a case of chronic suppurative otitis media were included in the study. The disk diffusion method was used on Mueller-Hinton Agar to detect antimicrobial susceptibility. Multipledrugs were tested for checking antimicrobial susceptibility. The data were analyzed by using Statistical Package for the Social Sciences (SPSS) version 20. Results: The mean age of the study participants was 13.89 ± 12.37 years. The majority of participants were froma younger age group i.e. less than10 years of age with female predominance. It had been found that the majority of cases (51%) were having Staphylococcus aureus infestation followed by Pseudomonas aeruginosa, Escherichia coli and very few with Proteus mirabilis. On the other hand, multiple drugs were tested to find out the antimicrobial susceptibility among the cases of chronic otitis media and the results reported that all the bacterial species were susceptible to Ceftriaxone while the other antibiotics were havinga variable response for different strains of bacteria. Conclusion: Results concluded that the Staphylococcus aureus was the most common organism followed by Pseudomonas aeruginosa, Escherichia coli and very few with Proteus mirabilis. It is important to identify the pathogenic organism and its sensitivity pattern before prescribing any antibiotic.

scholarly journals CHRONIC SUPPURATIVE OTITIS MEDIA

2010 ◽  
Vol 17 (03) ◽  
pp. 420-424
Author(s):  
MUNIR LODHI ◽  
KAMRAN AZIZ ◽  
TEHMINA MUNIR ◽  
Hamza Lodhi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Proteus Mirabilis ◽  
Chronic Otitis Media ◽  
Initial Therapy ◽  
Tympanic Membrane Perforation ◽  
Military Hospital ◽  
Suppurative Otitis Media ◽  
Culture And Sensitivity

Objective: The aim of the study was to determine the most prevalent organisms in chronic otitis media in children and their susceptibility to various antibimicrobials so that an appropriate empiric antibiotic can be started promptly while awaiting the results of the culture and sensitivity. Design: Prospective observational study. Place and Duration of Study: The study was conducted at Combined Military Hospital Lahore from Jan 2006 to Dec 2007. Subject and Methods: A total of 156 patients less than 15 years of age who were having discharge from one or both ears for at least 1 week with tympanic membrane perforation were included in the study.A sample of the ear discharge was collected on the swab and cultured on appropriate media. The Gram positive organisms were identified on the basis of Gram staining, catalase and coagulase test, the Gram negative organisms were identified by API 20e. A total of 156 patients were included in the study of which 96(61.5%) were males and 59(37.8%) were females. The organisms isolated were Staphylococcus aureus in 79(50.6%), Pseudomonas aeruginosa in 45 (28.8%) patients and Proteus mirabilis 17 (10.9%), Escherichia coli, Acinetobacter sp and streptocci were isolated in occasional patients. Staphylococcus aureus was the commonest organism isolated followed by Pseudomonas aeruginosa and Proteusmirabilis Fifty four. (34.6%)of Staphylococcus aureus were sensitive to gentamicin, fifty two (33.3%) to ciprofloxacin and 42 (26.9%)% to both ciprofloxacin and gentarnicin. Among the Pseudomonas aeruginosa isolates 40 (25.6%) were sensitive to gentarnicin, 27(17.3%) to ciprofloxacin and 22 (14.1%) to both ciprofloxacin and gentarnicin. Out of 156 patients 91(58.3%) had isolates which were sensitive to ciprofloxacin and 62(39.7%) were resistant to it. One hundred fourteen patients (73.1%)had isolates which were sensitive to gentamicin whereas 33(21.2%) were resistant. Similarly 35 isolates (22.4%) were sensitive to ulphamethoxazole/trimethoprim whereas 66(42.3%) were resistant. Among Proteus mirabilis isolates 12 were sensitive to gentamicin, 11 were sensitive to ciprofloxacin and 11 were sensitive to both ciprofloxacin and gentamicin. Conclusion: Ciprofoxacin ear drops can be recommended to be given empirically in children with chronic discharging ears. The initial therapy can be modified and appropriate therapy started if the result of the culture and sensitivity report shows the isolate to be resistant to the antibiotic started empirically.

scholarly journals Staphylococcus aureus and Pseudomonas aeruginosa in Tubotympanic Chronic Suppurative Otitis Media Patients in Purwokerto, Indonesia

2020 ◽  
Vol 12 (4) ◽  
pp. 340-348
Author(s):  
Daniel Joko Wahyono ◽  
Anton Budhi Darmawan ◽  
Leader Alfason ◽  
Reinhard Simbolon ◽  
Siwi Pramatama Mars Wijayanti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Antimicrobial Susceptibility ◽  
Chronic Suppurative Otitis Media ◽  
Molecular Tools ◽  
Suppurative Otitis Media ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

BACKGROUND: Chronic Suppurative Otitis Media (CSOM) causes hearing impairment and frequently occurred in low-income country where medical care and personal hygiene are poor. Staphylococcus aureus and Pseudomonas aeruginosa are the most common cause of CSOM. We investigated prevalence and antimicrobial susceptibility of S. aureus and P. aeruginosa from tubotympanic CSOM patients in tertiary hospital, Purwokerto, Indonesia in 2016-2017.METHODS: Ear swab specimens were collected from patients with tubotympanic CSOM. S. aureus and P. aeruginosa were isolated and identified by culture, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and molecular tools. Antimicrobial susceptibility testing was performed using the disk diffusion method.RESULTS: Out of ear swabs from 34 patients with tubotympanic CSOM, P. aeruginosa and S. aureus were identified in 35%patients. No Methicillin-resistant S. aureus (MRSA) strain was found from the ear swabs of the patients with tubotympanic CSOM. Bacterial identification using the MALDI-TOF MS was concordantly with culture and molecular tools. All S. aureus isolates showed full susceptibility to cefoxitin and trimethoprim-sulphamethoxazole. Resistance to tetracycline was common with only 64% of S. aureus strains being susceptible. Meanwhile, all P. aeruginosa strains were susceptible to cefepime, cetazidime, meropenem, gentamicin, and tobramycin.CONCLUSION: S. aureus and P. aeruginosa are found in patients with tubotympanic CSOM and still susceptible to different antibiotic agents. MALDI-TOF MS demonstrate rapid, accurate and robust to detect S. aureus and P. aeruginosa.KEYWORDS: Staphylococcus aureus, Pseudomonas aeruginosa, chronic tubotympanic suppurative otitis media

OTITIS MEDIA IN THE FIRST SIX WEEKS OF LIFE: DIAGNOSIS, BACTERIOLOGY, AND MANAGEMENT

PEDIATRICS ◽  
1972 ◽  
Vol 49 (2) ◽  
pp. 187-197
Author(s):  
Richard D. Bland
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Treatment Failure ◽  
Otitis Media ◽  
Congenital Malformations ◽  
Young Infant ◽  
Suppurative Otitis Media ◽  
Made In

Over a 1-year period, a diagnosis of suppurative otitis media was made in 21 infants less than 6 weeks of age, with symptomatology varying from mild rhinorrhea and cough to irritability and severe, protracted diarrhea. Only four infants presented with fever greater than 38°C. One-third of the patients had been born prematurely, 90% were bottle-fed, and only two had significant congenital malformations. Two-thirds of the babies with otitis of bacterial origin were male. Infants admitted to the study underwent diagnostic tympanoparacentesis, yielding purulent material and bacterial growth from all but three. The predominant organisms isolated were Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae. In cases of treatment failure, reinfection was most common with Pseudomonas aeruginosa. On the basis of disc sensitivities, only 61% of the exudates from which bacteria were isolated proved amenable to ampicillin therapy alone. Bacteria cultured from nasopharyngeal swabs of the same infants showed a correlation with ear isolations in only 28% of the cases. When a 10-day course of therapy was carried out utilizing antibiotics to which the organism was sensitive, there was a 22% failure rate, suggesting the possible need for more prolonged therapy with a parenterally-administered agent plus ampicillin in the management of otitis media in the neonate and young infant.

Bacteriology of Chronic Suppurative Otitis Media

1994 ◽  
Vol 103 (10) ◽  
pp. 771-774 ◽  
Author(s):  
Mustafa Erkan ◽  
Erdoǧan Sevük ◽  
Tahsin Aslan ◽  
Ercihan Güney
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Otitis Media ◽  
Bacteroides Fragilis ◽  
Chronic Otitis Media ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Bacteroides Fragilis Group ◽  
Gram Positive Cocci

Aspiration of exudate through an open perforation was performed in 183 patients with chronic otitis media. The pus was cultured aerobically and anaerobically. Aerobes only were isolated from 71 patients (39%); 20 patients (11%) had only anaerobes; and 91 patients (50%) had both aerobes and anaerobes. Only 1 specimen had no growth. There were 259 aerobic isolates. Pseudomonas aeruginosa was recovered from 68 patients. Other aerobes commonly recovered included Staphylococcus aureus and Klebsiella pneumoniae. There were 178 anaerobic isolates. Only anaerobic gram-positive cocci were isolated in 20 instances. Sixty-three Bacteroides isolates were recovered, including 12 Bacteroides fragilis group and 21 Bacteroides melaninogenicus.

scholarly journals Microbiology and antibiotic sensitivity of uncomplicated chronic suppurative otitis media at Dr. George Mukhari Academic Hospital, South Africa

2021 ◽  
Vol 7 (4) ◽  
pp. 565
Author(s):  
Monica Kotu ◽  
Ian Paul Olwoch
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Proteus Mirabilis ◽  
Antibiotic Sensitivity ◽  
Chronic Suppurative Otitis Media ◽  
Older Children ◽  
Suppurative Otitis Media ◽  
Sensitivity Pattern ◽  
Age Range

<p class="abstract"><strong>Background:</strong> The study was conducted to assess the type and frequency of isolation of different microorganisms in uncomplicated chronic suppurative otitis media (CSOM) and their antibiotic sensitivity in our institution.</p><p class="abstract"><strong>Methods:</strong> A total of 88 consecutive patients with unilateral or bilateral active, chronic suppurative otitis media attending outpatient department at DGMAH were included in the study after obtaining an informed consent. There were 55 males (62.5%) and 33 females (37.9%) with age range between 6 months and 76 years. Pus swabs were taken through the perforation site and from the promontory after ear mopping under direct vision.  </p><p><strong>Results:</strong> Children less than 5 years were more affected (23.9%) than older children 5-10 years (13.6%) and 11-15 years (11.45%). One hundred and six microorganisms were isolated from analysis of cultures obtained from 72 patients. Seven cultures were negative (5.9%), 8 specimens were contaminated (6.7%) and 1 specimen was lost (1.1%). <em>Pseudomonas aeruginosa (</em>24.0%<em>) </em>was the most common isolate, followed by <em>Staphylococcus aureus </em>(17%) and <em>Proteus mirabilis </em>(10%). Drug sensitivity pattern showed that Piperacillin-tazobactam was effective against the majority of <em>Pseudomonas aeruginosa </em>isolates at 72%, followed by both Gentamicin and Ceftazidime at 64% and Ciprofloxacin at 48%. <em>Staphylococcus aureus </em>isolates were sensitive to Erythromycin (77%), Cloxacillin and Clindamycin at 72%. <em>Proteus mirabilis </em>was sensitive to Cefuroxime (91%), Co-amoxiclav (72.8%).</p><p class="abstract"><strong>Conclusions:</strong> Isolation rate and susceptibility patterns in CSOM, suggest a need for regular surveillance to monitor antimicrobial resistance and to guide antibacterial therapy.</p><p class="abstract"> </p><h1> </h1>

Bacteriological study and antibiotic sensitivity profile in patients with ear discharge visiting ENT OPD at tertiary care center

2017 ◽  
Vol 4 (1) ◽  
pp. 237
Author(s):  
Kailas R. Patel ◽  
Alpesh D. Fefar ◽  
Paresh J. Khavdu ◽  
Sejal N. Mistry ◽  
Manish R. Mehta
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Antibiotic Sensitivity ◽  
Polymyxin B ◽  
Chronic Suppurative Otitis Media ◽  
Purulent Discharge ◽  
Suppurative Otitis Media

<p class="abstract"><strong>Background:</strong> <span lang="EN-IN">Otitis media (OM) is a big menace in countries like India. Chronic suppurative otitis media (CSOM) is chronic inflammation of middle ear, which affects the tympanic membrane, middle ear mucosa and other middle ear structures characterised by the presence of persistent perforation or persistent mucoid or muco-purulent discharge for at least 8 weeks. Variety of organisms founds in patients suffering from chronic otitis media like, <em>Pseudomonas</em>, <em>Staphylococcus aureus</em>, <em>Proteus mirabilis</em>, <em>Klebsiella pneumonia</em> and <em>Escherichia coli</em> found. The pattern of organisms varies in different geographical areas. The objectives of the study were to isolate bacteria from patients with ear discharge, to find out antibiotic sensitivity of isolated organisms</span></p><p class="abstract"><strong>Methods:</strong> <span lang="EN-IN">This is an observational cross sectional study done in the OPD department of otorhinolaryngology, at P.D.U medical college and hospital, Rajkot, for a period of six months from January 2017 to June 2017. A total number 200 patients of both gender between 5 to 74 years who visited the OPD department of otorhinolaryngology with chronic ear discharge taken for study.  </span></p><p class="abstract"><strong>Results:</strong> <span lang="EN-IN">Out of 200 patients of 5-74 years with chronic suppurative otitis media were examined and out of this Hundred and twelve (54%) of the patients were males with mean age of 33.44. <em>Pseudomonas aeruginosa</em> was the commonest identified organisms the sensitivity pattern highly favoured Pipercillin, Amikacin and Polymyxin B, Cefoparazone. </span></p><p class="abstract"><strong>Conclusions:</strong> <span lang="EN-IN">Chronic otitis media is more prevalent in males than females. Commonest organisms found were <em>Pseudomonas aeruginosa</em> followed by <em>Staphylococcus aureus. </em>Piperacillin, Amikacin, Gentamicin, Polymyxin B and Cefoperazone were found most effective agents against <em>Pseudomonas aeruginosa</em>.</span></p>

scholarly journals Efeito antimicrobiano do extrato de Cranberry sobre micro-organismos causadores de infecção urinária

2016 ◽  
Vol 11 (31) ◽  
pp. 113-122
Author(s):  
Carla Franco Porto Belmont Souza ◽  
Luiz Eduardo Souza da Silva Irineu ◽  
Renan Silva De Souza ◽  
Renato da Silva Teixeira ◽  
Ivina Sanches Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Enterococcus Faecium ◽  
Vaccinium Macrocarpon ◽  
Mueller Hinton

A resistência microbiana tem se mostrado um problema de proporções mundiais, causando estado de morbidade e mortalidade em diversos pacientes. Em vista disso, tem crescido a busca por métodos alternativos naturais de profilaxia. A investigação clínica sugere que o Extrato de Cranberry está entre as melhores propostas de prevenção natural. O Cranberry (Vaccinium macrocarpon) é um fruto que tem crescido comercialmente pelo sabor e propriedades benéficas à saúde. Dentre as formas comercializadas estão: o suco, o chá e as cápsulas contendo o extrato seco. A ação desta planta está relacionada ao tratamento de doenças do trato urinário, por possuir substâncias que inibem a adesão bacteriana ao epitélio do trato urinário, dificultando sua proliferação e reprodução. Dentre todas as infecções relacionadas à assistência a saúde, a Infecção do Trato Urinário é a mais frequentemente associada a procedimentos invasivos. Se não for tratada, pode resultar em complicações como pielonefrite aguda, bacteremia e pionefrose. Portanto, cranberry pode ser uma nova alternativa para o combate das infecções uroepiteliais, por ser um produto natural de preço acessível, e com formas de comercialização diversificada, ao contrário dos antimicrobianos convencionais, que por sua vez são caros e podem acabar causando resistência nos micro-organismos. Este trabalho teve como objetivo avaliar in vitro a atividade antimicrobiana do extrato de Cranberry, adquirido em farmácia de manipulação, sobre 8 micro-organismos isolados de infecções urinárias. As cepas utilizadas, adquiridas da coleção da FIOCRUZ, foram: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Serratia marscecens, Staphylococcus aureus, Enterococcus faecalis e Enterococcus faecium. No estudo, foram utilizados o caldo Mueller Hinton (MH), Extrato de Cranberry e as bactérias patogênicas. O ensaio foi realizado em triplicata, com o uso de um controle de crescimento dos micro-organismos e o experimento para avaliação do crescimento bacteriano na presença do extrato. A turbidez foi medida com o auxílio de um espectrofotômetro, no comprimento de onda de 600 nm, antes e após 24 horas de incubação à 37 ºC. O procedimento forneceu a Densidade Ótica, do qual possibilitou a identificação da inibição microbiana. Para análise estatística foi utilizado o Teste t de Student. O Extrato de Cranberry apresentou atividade antimicrobiana sobre as bactérias Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Serratia marscecens e Enterococcus faecalis (p < 0,05), confirmando seu efeito benéfico em infecções urinárias. No entanto, não teve efeito inibitório significativo sobre Pseudomonas aeruginosa, Proteus mirabilis e Enterococcus faecium (p > 0,05).

Aerobic Bacteriology of Chronic Suppurative Otitis Media without Cholesteatoma in Children

1992 ◽  
Vol 101 (10) ◽  
pp. 866-869 ◽  
Author(s):  
Dan M. Fliss ◽  
Naftali Meidan ◽  
Ron Dagan ◽  
Alberto Leiberman
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Prospective Study ◽  
Otitis Media ◽  
Granulation Tissue ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Gram Negative ◽  
Nonsurgical Management ◽  
A Prospective Study

Medical management of chronic suppurative otitis media without cholesteatoma in children was shown recently to be efficacious. We undertook a prospective study in order to determine 1) the organisms associated with the disease and their sensitivity to various antibiotics and 2) their association with clinical variables. Pseudomonas was isolated from 84% of the patients, enteric gram-negative bacilli from 32%, Staphylococcus aureus from 20%, streptococci from 14%, and Hemophilus influenzae from 15%. Pseudomonas aeruginosa was the sole isolate in 48 of the 128 patients (38%). The sensitivity of P aeruginosa (78 isolates) was 100% (of isolates) to mezlocillin and ciprofloxacin, 99% to tobramycin and ceftazidime, 97% to piperacillin, 94% to gentamicin, and 78% to ceftriaxone. The isolated organisms and their sensitivity to antibiotics were not associated with age, sex, duration of otorrhea, or the presence of granulation tissue or polyps. We believe that our data may contribute to the appropriate choice of antibiotics when nonsurgical management of chronic suppurative otitis media without cholesteatoma is considered.

scholarly journals Microbiological Study of Diabetic Foot Ulcer

2019 ◽  
Vol 10 (1) ◽  
pp. 50-55
Author(s):  
Mohammuddunnobi ◽  
Tasnuva Jahan ◽  
Abdullah Al Amin
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Klebsiella Pneumonia ◽  
Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening diabetic infections are usually polymicrobial involving multiple aerobic and anaerobic organisms. Methodology: The present study was a cross sectional study, conducted in the department of surgery and microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September' 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is 'any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus'. Results: Majority 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillin-clavulanic acid, amikacin, piperacillin-tazobactam. In Coagulasenegative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem. Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Escherichia coli and Citrobacter spp. Anwer Khan Modern Medical College Journal Vol. 10, No. 1: Jan 2019, P 50-55

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