scholarly journals Staphylococcus aureus and Pseudomonas aeruginosa in Tubotympanic Chronic Suppurative Otitis Media Patients in Purwokerto, Indonesia

2020 ◽  
Vol 12 (4) ◽  
pp. 340-348
Author(s):  
Daniel Joko Wahyono ◽  
Anton Budhi Darmawan ◽  
Leader Alfason ◽  
Reinhard Simbolon ◽  
Siwi Pramatama Mars Wijayanti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Antimicrobial Susceptibility ◽  
Chronic Suppurative Otitis Media ◽  
Molecular Tools ◽  
Suppurative Otitis Media ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

BACKGROUND: Chronic Suppurative Otitis Media (CSOM) causes hearing impairment and frequently occurred in low-income country where medical care and personal hygiene are poor. Staphylococcus aureus and Pseudomonas aeruginosa are the most common cause of CSOM. We investigated prevalence and antimicrobial susceptibility of S. aureus and P. aeruginosa from tubotympanic CSOM patients in tertiary hospital, Purwokerto, Indonesia in 2016-2017.METHODS: Ear swab specimens were collected from patients with tubotympanic CSOM. S. aureus and P. aeruginosa were isolated and identified by culture, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and molecular tools. Antimicrobial susceptibility testing was performed using the disk diffusion method.RESULTS: Out of ear swabs from 34 patients with tubotympanic CSOM, P. aeruginosa and S. aureus were identified in 35%patients. No Methicillin-resistant S. aureus (MRSA) strain was found from the ear swabs of the patients with tubotympanic CSOM. Bacterial identification using the MALDI-TOF MS was concordantly with culture and molecular tools. All S. aureus isolates showed full susceptibility to cefoxitin and trimethoprim-sulphamethoxazole. Resistance to tetracycline was common with only 64% of S. aureus strains being susceptible. Meanwhile, all P. aeruginosa strains were susceptible to cefepime, cetazidime, meropenem, gentamicin, and tobramycin.CONCLUSION: S. aureus and P. aeruginosa are found in patients with tubotympanic CSOM and still susceptible to different antibiotic agents. MALDI-TOF MS demonstrate rapid, accurate and robust to detect S. aureus and P. aeruginosa.KEYWORDS: Staphylococcus aureus, Pseudomonas aeruginosa, chronic tubotympanic suppurative otitis media

Aerobic Bacteriology of Chronic Suppurative Otitis Media without Cholesteatoma in Children

1992 ◽  
Vol 101 (10) ◽  
pp. 866-869 ◽  
Author(s):  
Dan M. Fliss ◽  
Naftali Meidan ◽  
Ron Dagan ◽  
Alberto Leiberman
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Prospective Study ◽  
Otitis Media ◽  
Granulation Tissue ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Gram Negative ◽  
Nonsurgical Management ◽  
A Prospective Study

Medical management of chronic suppurative otitis media without cholesteatoma in children was shown recently to be efficacious. We undertook a prospective study in order to determine 1) the organisms associated with the disease and their sensitivity to various antibiotics and 2) their association with clinical variables. Pseudomonas was isolated from 84% of the patients, enteric gram-negative bacilli from 32%, Staphylococcus aureus from 20%, streptococci from 14%, and Hemophilus influenzae from 15%. Pseudomonas aeruginosa was the sole isolate in 48 of the 128 patients (38%). The sensitivity of P aeruginosa (78 isolates) was 100% (of isolates) to mezlocillin and ciprofloxacin, 99% to tobramycin and ceftazidime, 97% to piperacillin, 94% to gentamicin, and 78% to ceftriaxone. The isolated organisms and their sensitivity to antibiotics were not associated with age, sex, duration of otorrhea, or the presence of granulation tissue or polyps. We believe that our data may contribute to the appropriate choice of antibiotics when nonsurgical management of chronic suppurative otitis media without cholesteatoma is considered.

scholarly journals Emergence of antibiotic resistance in bacteria isolated from tubotympanic type of chronic suppurative otitis media in Chhattisgarh

2019 ◽  
Vol 5 (6) ◽  
pp. 1674
Author(s):  
B. R. Singh ◽  
Sagarika Pradhan ◽  
R. Murthy ◽  
Ekta Agrawal ◽  
Rekha Barapatre ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Antibiotic Sensitivity ◽  
Chronic Suppurative Otitis Media ◽  
Causative Organism ◽  
Suppurative Otitis Media ◽  
Major Health Problem ◽  
Common Organism ◽  
Life Threatening

<p class="abstract"><strong>Background:</strong> Chronic suppurative otitis media (CSOM) is a common chronic ear disease and a major health problem in developing countries causing deafness and life threatening complications like meningitis and brain abscess. Early and effective treatment based on the knowledge of causative organism and their antibiotic sensitivity is essential for immediate clinical recovery.</p><p class="abstract"><strong>Methods:</strong> Total of 152 clinically diagnosed patients with CSOM safe were enrolled in the study and two swab of the discharge ear were obtained from each patient under aseptic precaution. Microbiological study was done according to standard operative guidelines.  </p><p class="abstract"><strong>Results:</strong> Out of 152 patients 93 male and 59 were female, majority of the patient seen below 30 year of age. Most common organism found was <em>Staphylococcus aureus </em>(43.2%) and <em>Pseudomonas aeruginosa </em>(27.9%). <em>Staphylococcus aureus</em> was sensitive to vancomycin (98%) and linezolide (100%) and <em>Pseudomonas aeruginosa </em>was found highly sensitive to Imipenem cilastin (84.85%) and Imipenem (66.7%).</p><p class="abstract"><strong>Conclusions:</strong> In our study gram positive <em>Staphylococcus aureus</em> (43.2%) and gram negative bacilli <em>P. aeuroginosa</em> (27.9%) was the most common organism was found and this <em>P. aeruginosa</em> were hospital acquired infections. We found out high resistant to Cefepime, ceftazidime, ciprofloxacin and levofloxacin and aztreonam.</p>

scholarly journals Chronic Suppurative Otitis Media: Bacteriology and Drug Sensitivity Patterns at the Quirino Memorial Medical Center (2004-2005): A Preliminary Study

2006 ◽  
Vol 21 (1-2) ◽  
pp. 20-23 ◽  
Author(s):  
Patricia N. Ayson ◽  
Jan Eero G. Lopez ◽  
Erasmo Gonzalo DV. Llanes
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Drug Sensitivity ◽  
Medical Center ◽  
Bacterial Pathogens ◽  
Chronic Suppurative Otitis Media ◽  
Cross Sectional Survey ◽  
Suppurative Otitis Media ◽  
Sensitivity Pattern

Objectives: To identify the bacterial pathogens associated with chronic suppurative otitis media and their antimicrobial sensitivity and resistance   Study Design: Cross-sectional survey   Setting: The study was carried out from July 2004-July 2005 at the outpatient clinic of a government tertiary hospital.   Number of subjects: A total of 32 patients (54 ears) with unilateral or bilateral active chronic suppurative otitis media.   Results: Bacterial pathogens of 54 ear discharge samples from CSOM were studied. Among them, 42 (78%) were pure cultures and 9 (16.7%) were mixed, only 3 (5.6%) of the submitted samples had no growth. There were 42 pure isolates, the most common of which was Staphylococcus aureus 21 (50%), followed by Pseudomonas aeruginosa 14 (33.3%). Drug sensitivity pattern of Staphylococcus aureus showed that 61.9% were resistant to Penicillin while more than 90% were sensitive to Aminoglycosides and Clindamycin. Pseudomonas aeruginosa was resistant to penicillin in 64.3% of cases and Ciprofloxacin was active against pseudomonas in 85.7%.   Conclusion: The most common bacterial pathogens from CSOM include Staphylococcus aureus and Pseudomonas aeruginosa. Majority of the isolates of Staphylococcus aureus were resistant to penicillin. Aminoglycosides, macrolides and quinolones were effective against most of the isolates of Staphylococcus aureus.   Keywords: chronic otitis media, perforated tympanic membrane, chronic aural discharge    

Anaerobes and Fungi in Chronic Suppurative Otitis Media

1997 ◽  
Vol 106 (8) ◽  
pp. 649-652 ◽  
Author(s):  
A. Olu Ibekwe ◽  
Zain Al Shareef ◽  
Ashraf Benayam
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Aspergillus Species ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Pure Cultures ◽  
Pathogenic Organisms

Microbiology of 102 ears with chronic suppurative otitis media was studied for aerobes, anaerobes, and fungi. Forty-four percent were pure cultures, 33.3% were mixed, and 18.6% had no growth. Seventy-four percent were aerobes, 25% fungi, and only 0.9% anaerobes. Pseudomonas aeruginosa (22.5%) was the most common isolate, followed by Staphylococcus aureus and the Aspergillus species. The possible reasons for low yield of anaerobes and the pathogenic roles of anaerobes and fungi in chronic suppurative otitis media are discussed. It is advocated that in investigating pathogenic organisms in chronic suppurative otitis media, requests should include anaerobes and fungi.

scholarly journals Aerobic bacteriology of Chronic Suppurative Otitis Media (CSOM) in a tertiary care hospital: A retrospective study

2012 ◽  
Vol 7 (2) ◽  
pp. 1-8 ◽  
Author(s):  
RK Sanjana ◽  
YI Singh ◽  
NS Reddy
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Tertiary Care ◽  
Chronic Suppurative Otitis Media ◽  
Diffusion Method ◽  
National Committee ◽  
Care Hospital ◽  
Suppurative Otitis Media ◽  
Sensitivity Pattern

The present study was carried out to determine the aerobic microorganisms involved and their antibiotic sensitivity pattern in patients with Chronic Suppurative Otitis Media (CSOM) and to provide a guideline for empirical antibiotic therapy.Between March 2009 to February 2011, the ear discharge samples submitted at the microbiology laboratory were processed aerobically and all the isolates were included in this study. All organisms were identified morphologically and biochemically by standard laboratory procedures and antibiotic susceptibility pattern was determined by modified Kirby Bauer disc diffusion method as per National Committee for Clinical Laboratory Standard recommendations.Out of a total of 214 samples, microbiological culture was yielded from 202 (94.3%) specimens. Single organism were isolated from 168 (78.5%) of the culture positive specimens, while the remaining 34 (15.9%) had two or more organisms isolated. Pseudomonas aeruginosa 59 (35.1%) was the most common isolate, followed by Staphylococcus aureus 42 (25.0%) including 5 (3%) of the Methicillin Resistant Staphylococcus Aureus (MRSA). Antibiotic sensitivities of Pseudomonas aeruginosa showed that 93.2% isolates were sensitive to tobramycin, whereas, 91.5% isolates were sensitive to ceftazidime and 77.9% to amikacin.Only 50.8% of the isolates of Pseudomonas aeruginosa were sensitive to ciprofloxacin and 25.4% to gentamicin. For Staphylococcus aureus (other than MRSA) 95.2% were sensitive to cloxacillin, 83.3% to gentamicin and 78.5% to erythromycin. Only 07.1% were sensitive to ampicillin and 26.1% to ciprofloxacin.Pseudomonas aeruginosa was the most common isolate followed by Staphylococcus aureus. More than 90% of Pseudomonas and 90% of Staph aureus were sensitive to tobramycin and cloxacillin respectively. Therefore, these two drugs may be included in the formulary to cover the most common aerobic isolates involved in CSOM.Journal of College of Medical Sciences-Nepal, 2011, Vol-7, No-2, 1-8DOI: http://dx.doi.org/10.3126/jcmsn.v7i2.6673

Bacteriology of Chronic Suppurative Otitis Media

1994 ◽  
Vol 103 (10) ◽  
pp. 771-774 ◽  
Author(s):  
Mustafa Erkan ◽  
Erdoǧan Sevük ◽  
Tahsin Aslan ◽  
Ercihan Güney
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Otitis Media ◽  
Bacteroides Fragilis ◽  
Chronic Otitis Media ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Bacteroides Fragilis Group ◽  
Gram Positive Cocci

Aspiration of exudate through an open perforation was performed in 183 patients with chronic otitis media. The pus was cultured aerobically and anaerobically. Aerobes only were isolated from 71 patients (39%); 20 patients (11%) had only anaerobes; and 91 patients (50%) had both aerobes and anaerobes. Only 1 specimen had no growth. There were 259 aerobic isolates. Pseudomonas aeruginosa was recovered from 68 patients. Other aerobes commonly recovered included Staphylococcus aureus and Klebsiella pneumoniae. There were 178 anaerobic isolates. Only anaerobic gram-positive cocci were isolated in 20 instances. Sixty-three Bacteroides isolates were recovered, including 12 Bacteroides fragilis group and 21 Bacteroides melaninogenicus.

scholarly journals Microbiology and antibiotic sensitivity of uncomplicated chronic suppurative otitis media at Dr. George Mukhari Academic Hospital, South Africa

2021 ◽  
Vol 7 (4) ◽  
pp. 565
Author(s):  
Monica Kotu ◽  
Ian Paul Olwoch
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Media ◽  
Proteus Mirabilis ◽  
Antibiotic Sensitivity ◽  
Chronic Suppurative Otitis Media ◽  
Older Children ◽  
Suppurative Otitis Media ◽  
Sensitivity Pattern ◽  
Age Range

<p class="abstract"><strong>Background:</strong> The study was conducted to assess the type and frequency of isolation of different microorganisms in uncomplicated chronic suppurative otitis media (CSOM) and their antibiotic sensitivity in our institution.</p><p class="abstract"><strong>Methods:</strong> A total of 88 consecutive patients with unilateral or bilateral active, chronic suppurative otitis media attending outpatient department at DGMAH were included in the study after obtaining an informed consent. There were 55 males (62.5%) and 33 females (37.9%) with age range between 6 months and 76 years. Pus swabs were taken through the perforation site and from the promontory after ear mopping under direct vision.  </p><p><strong>Results:</strong> Children less than 5 years were more affected (23.9%) than older children 5-10 years (13.6%) and 11-15 years (11.45%). One hundred and six microorganisms were isolated from analysis of cultures obtained from 72 patients. Seven cultures were negative (5.9%), 8 specimens were contaminated (6.7%) and 1 specimen was lost (1.1%). <em>Pseudomonas aeruginosa (</em>24.0%<em>) </em>was the most common isolate, followed by <em>Staphylococcus aureus </em>(17%) and <em>Proteus mirabilis </em>(10%). Drug sensitivity pattern showed that Piperacillin-tazobactam was effective against the majority of <em>Pseudomonas aeruginosa </em>isolates at 72%, followed by both Gentamicin and Ceftazidime at 64% and Ciprofloxacin at 48%. <em>Staphylococcus aureus </em>isolates were sensitive to Erythromycin (77%), Cloxacillin and Clindamycin at 72%. <em>Proteus mirabilis </em>was sensitive to Cefuroxime (91%), Co-amoxiclav (72.8%).</p><p class="abstract"><strong>Conclusions:</strong> Isolation rate and susceptibility patterns in CSOM, suggest a need for regular surveillance to monitor antimicrobial resistance and to guide antibacterial therapy.</p><p class="abstract"> </p><h1> </h1>

Sign in / Sign up

Export Citation Format

Share Document