scholarly journals Evaluation of A Navigator's Mental Workload Based on Salivary Amylase Activity

2009 ◽  
Vol 121 (0) ◽  
pp. 1-6 ◽  
Author(s):  
Koji MURAI ◽  
Shin-ichi WAKIDA ◽  
Takashi MIYADO ◽  
Yuji HAYASHI
Keyword(s):  
Amylase Activity ◽  
Mental Workload ◽  
Salivary Amylase

Toward New Practical Education Based on ProfessionalKansei

2011 ◽  
Vol 15 (3) ◽  
pp. 370-376 ◽  
Author(s):  
Koji Murai ◽  
◽  
Yuji Hayashi ◽  
Kei Kuramoto ◽  
Takayuki Fujita ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Physical Performance ◽  
Amylase Activity ◽  
Mental Workload ◽  
Salivary Amylase ◽  
Professional Performance ◽  
Physiological Indices ◽  
Practical Education ◽  
Ship Handling

To evaluate the ship-handling skills andkanseiof professionals and students, we studied physical performance and mental workload using a simulator and an actual ship. We demonstrated the effects of physiological indices based on professional performance and features as seen from their mental workload with heart rate variability, nasal temperature, and salivary amylase activity, and considered new practical education using professionalkansei.

Enhancing maritime education and training

2009 ◽  
Vol 6 (4) ◽  
pp. 293-302 ◽  
Author(s):  
Koji Murai ◽  
Shin‐Ichi Wakida ◽  
Keiichi Fukushi ◽  
Yuji Hayashi ◽  
Laurie C. Stone
Keyword(s):  
Amylase Activity ◽  
Mental Workload ◽  
Effective Index ◽  
Future Research ◽  
Salivary Amylase ◽  
Content Type ◽  
Maritime Education ◽  
Safe Navigation ◽  
Narrow Passage ◽  
Ship Handling

PurposeThe purpose of this paper is to propose that the measurement of salivary amylase activity is an effective index to evaluate the stress of a ship navigator for safe navigation training and education.Design/methodology/approachEvaluation comes from the simulator and actual on‐board experiments. The subjects are real captains who have unlimited licenses and cadets who are senior students at Kobe University, navigation course. Stress is evaluated for several situations where a ship navigator makes a lot of decisions, in this case in a narrow passage, entering a port and leaving a port.FindingsSalivary amylase activity occurs when a ship navigator makes a decision regarding ship handling and collision avoidance. By measuring salivary amylase activity when a student is under duress, cadets' ship‐handling training can be evaluated while onboard a vessel.Research limitations/implicationsFuture research will develop cross‐indices with the salivary amylase activity and other physiological indices (nasal temperature and heart rate variability (R‐R interval)), complementary to each other. The salivary amylase activity registers the stress quickly on the spot. Then the nasal temperature and R‐R interval registers the trend and the quick response to the stress (mental workload).Practical implicationsThe paper describes an effective index which is useful for evaluating a ship navigator's stress for safe navigation.Originality/valueShip navigator's skill and cadet's on‐board training have been evaluated according to performance and a questionnaire as a quantitative evaluation; moreover, stress is evaluated using salivary amylase activity.

Rapid quantitative, specific measurement of pancreatic amylase in serum with use of a monoclonal antibody.

1985 ◽  
Vol 31 (8) ◽  
pp. 1283-1286 ◽  
Author(s):  
T E Mifflin ◽  
D C Benjamin ◽  
D E Bruns
Keyword(s):  
Polyvinylidene Fluoride ◽  
Amylase Activity ◽  
Kinetic Method ◽  
Reference Intervals ◽  
Pancreatic Amylase ◽  
Salivary Amylase ◽  
Electrophoretic Method ◽  
Standard Curve ◽  
A Value ◽  
Analytical Recovery

Abstract In this rapid quantitative assay for pancreatic alpha-amylase (EC 3.2.1.1) in serum, we precipitate salivary amylases by 10-min incubation with monoclonal anti-salivary amylase antibody immobilized on particles of polyvinylidene fluoride. We then centrifuge the serum mixture and measure the pancreatic amylase activity remaining in the supernate by a kinetic method. The assay requires 50 microL of serum and the standard curve is linear to at least 1300 U of pancreatic amylase per liter of serum. CVs were 1.3% within-run, 6-8% day-to-day. Apparent analytical recovery of pancreatic amylase activity added to serum was 101% +/- 2%. Addition of purified salivary amylase, 356 U/L, to sera gave a value for apparent pancreatic amylase of less than 4 U/L, or 1% of the added salivary amylase activity. This assay correlated well with an electrophoretic method (slope, 0.97-0.99; intercept, 0.5 to -4 U/L; correlation coefficient, 0.946-0.990; and standard error of the estimate 3-5 U/L). Estimated normal reference intervals with maltotetraose as substrate were: total amylase, 39-118 U/L; pancreatic amylase, 11-50 U/L; and salivary amylase, 18-79 U/L.

Effect of diet in West Africa on human salivary amylase activity

1972 ◽  
Vol 17 (10) ◽  
pp. 1415-1420 ◽  
Author(s):  
B. Wesley-Hadzija ◽  
H. Pigon
Keyword(s):  
West Africa ◽  
Amylase Activity ◽  
Salivary Amylase

Determination of Serum and Urine Amylase with Use of Procion Brilliant Red M-2BS Amylopectin

1971 ◽  
Vol 17 (4) ◽  
pp. 311-315 ◽  
Author(s):  
Sylvan M Sax ◽  
Anna B Bridgwater ◽  
John J Moore
Keyword(s):  
Amylase Activity ◽  
Reference Method ◽  
Monomethyl Ether ◽  
Final Solution ◽  
Salivary Amylase ◽  
Ethylene Glycol Monomethyl Ether ◽  
Glycol Monomethyl Ether ◽  
Urine Amylase ◽  
Pancreatic Extract

Abstract We describe a sensitive, precise assay of serum or urine amylase activity, with use of a new substrate, Procion Brilliant Red M-2BS—Amylopectin. After 0.2 ml of serum or urine is incubated with substrate for 10 min at 37°C, ethylene glycol monomethyl ether is added to precipitate proteins and larger substrate particles. Clarity and chromogenicity of the final solution are not sensitive to small changes in temperature or concentrations of the various reagents. No interferences necessitating preparation of specimen blanks have been encountered. Human salivary amylase, assayed by a reference saccharogenic method, is used for calibration. When read against the resulting curve, normal sera and urines give activities comparable to those obtained with the reference method. Human pancreatic extract, sera and urines from pancreatitis patients, and macroamylasemia serum show higher activities by the proposed method.

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