In situ bioremediation of Fenton’s reaction–treated oil spill site, with a soil inoculum, slow release additives, and methyl-β-cyclodextrin

A residential lot impacted by spills from a leaking light heating oil tank was treated with a combination of chemical oxidation and bioremediation to avoid technically challenging excavation. The tank left emptied in the ground was used for slow infiltration of the remediation additives to the low permeability, clayey soil. First, hydrogen peroxide and citrate chelate was added for Fenton’s reaction–based chemical oxidation, resulting in a ca. 50% reduction from the initial 25,000 mg/kg average oil concentration in the soil below the tank. Part of this was likely achieved through mobilization of oily soil into the tank, which was beneficial in regards to the following biological treatment. By first adding live bacteria in a soil inoculum, and then oxygen and nutrients in different forms, an approximately 90% average reduction was achieved. To further enhance the effect, methyl-β-cyclodextrin surfactant (CD) was added, resulting finally in a 98% reduction from the initial average level. The applicability of the surfactant was based on laboratory-scale tests demonstrating that CD promoted oil degradation and, unlike pine soap, was not utilized by the bacteria as a carbon source, and thus inhibiting degradation of oils regardless of the positive effect on biological activity. The effect of CD on water solubility for different hydrocarbon fractions was tested to serve as the basis for risk assessment requirements for authorizing the use of the surfactant at the site.

Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil

Crude oil pollution has consistently deteriorated all environmental compartments through the cycle of activities of the oil and gas industries. However, there is a growing need to identify microbes with catabolic potentials to degrade these pollutants. This research was conducted to identify bacteria with functional degradative genes. A crude oil-polluted soil sample was obtained from an aged spill site at Imo River, Ebubu, Komkom community, Nigeria. Bacteria isolates were obtained and screened for hydrocarbon degradation potential by turbidometry assay. Plasmid and chromosomal DNA of the potential degraders were further screened for the presence of selected catabolic genes (C230, Alma, Alkb, nahAC, and PAHRHD(GP)) and identified by molecular typing. Sixteen (16) out of the fifty (50) isolates obtained showed biodegradation activity in a liquid broth medium at varying levels. Bacillus cereus showed highest potential for this assay with an optical density of 2.450 @ 600 nm wavelength. Diverse catabolic genes resident in plasmids and chromosomes of the isolates and, in some cases, both plasmid and chromosomes of the same organism were observed. The C230 gene was resident in >50% of the microbial population tested, while other genes occurred in lower proportions with the least observed in nahAC and PAHRHD. These organisms can serve as potential bioremediation agents.

Subsea Crude Oil Spill Detection Using Robotic Systems

Underwater Robotic Oil Spill Surveillance (UROSS) system provides constant and autonomous spill surveillance for subsea pipelines. M900 embedded Radio Frequency Identification tags and readers are used to identify pipeline section for surveillance. GY-521 gyrometer and XL-MaxSonar-WR1 ultrasound sensor are used for autonomous navigation. Spills are detected using a METS methane sensor. After spill detection, images of spill site are captured with a L3C-400 Micro Ultras-Miniature Color Camera and spill location is obtained using GPS. Spill Images and location are transmitted to a remote PC on the nearest off shore platform using an Xbee Pro 900HP wireless connection. An Ocean Signal rescueME Personal Beacon Locator transmits a 406 MHz distress signal via satellite to emergency services communicating the identification of a spill. Power analysis showed the system’s ability to remain submerged and to provide surveillance for 100 m sections of a subsea pipeline once every 24 hrs for a month, and can be increased to every hour for 51 months.