A single-phase flow microfluidic cell sorter for multiparameter screening to assist the directed evolution of Ca2+ sensors

We introduce a single-phase flow microfluidic cell sorter with a two-point detection system capable of two-parameter screening to assist with directed evolution of a fluorescent protein based Ca2+ sensor expressed in bacterial cells.

Method to study particle flow bias at a channel bifurcation in a microfluidic device

We demonstrate an empirical method and a balanced random forest statistical analysis to study the efficacy of microfluidic cell sorter designs with an imbalanced distribution of outcomes. The study uses polystyrene beads as model cells and studies the effects of design variables on the outcome of the beads at a Y-shaped bifurcation.

Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response

We describe the use of μFACS to aid the directed evolution of a genetically encoded yellow fluorescent Ca2+indicator.