Multimodal imaging to detect in vivo responses to aflibercept therapy in a mouse model of type 3 neovascularization

Purpose: To characterize the response to aflibercept in a mouse model of type 3 neovascularization, the neoretinal vascularization (NRV) 2 mouse line. Methods: Twelve NRV2 mice were assigned to one of the following groups: (i) six mice were injected with aflibercept 3µg/g at postnatal day 15 (“aflibercept” group), and (ii) the remaining six mice did not receive any treatment (“placebo” group). Mice were examined at postnatal day 30 (p30) and 44 (p44). Results: The NRV mice’s retinas were characterized by regions of depigmentation that were topographically associated with hyperfluorescent lesions seen on fluorescein angiography (FA) images. On optical coherence tomography (OCT) images, intraretinal neovascularizations were visualized as hyperreflective lesions mainly localized within the outer plexiform and outer nuclear layers. The average number of intraretinal neovascular lesions visualized on FA at P30 was 5.0±2.2 in the “aflibercept” and 20.7±2.4 in the “placebo” groups (p<0.0001). At P44, the average number of intraretinal lesions was still lower in the “aflibercept” group, although this difference was not statistically significant (p=0.088). Conclusion: Aflibercept therapy was effective in inhibiting the pathologic angiogenesis in the NRV2 mouse model. However, the successive treatment washout resulted in an increase in lesions’ number.

UJI AKTIVITAS EKSTRAK DAUN MBOA (Melastomata malabatricum) TERHADAP HAMA GUDANG Callosobruchus sp (COLEOPTERA:BRUCHIDAE) PADA KACANG HIJAU (Phaseolus radiata L.)

Activity Test Extract Mboa Leaf (Melastomata Malabatrikum) against warehouse pest Callosobruchus Sp (Coleoptera : Bruchidae) Green bean (Phaseolus Radiata L.). Green beans are an important crop in the tropics and have long cultivated in Indonesia. Green bean production in quantity and quality is strongly influenced by the presence of pest and diseases. The damage caused by insects ensued can reach 70% (Kartasapoetra, 1987). Given the magnitude percentage damage inflicted by insects CallosobbrushusSp is a necessary control measure. This study aims to determine the activity of leaf extract Mboa (M. Mallabatricum) against warehouse pest Callosobruchus Sp. This study uses a CDR with four treatments (M25, M50, M75, M100) and repeated 10 times. The result showed extra activity Mboa leaves (M. Mallabatricum) against Callosobruchus Sp to test a contact poison can cause Callosobruchus Sp morality above 80% seen in the treatment of M50, M75, and M100 of 9.987%. repellant test showed a decrease traffic to continue successive treatment – participated in treatments M25 (61%), M50 (46%), M75 (43%), M100(34%) and mortality due to neurological disorders for M25 (7.34%), M50 (7.99%), M75 (8.54%), and M100 (9.21%). An antibody test can suppress high levels of oviposition in each treatment. M25 (31.21%), M50 (71.01%), M75(79.5%), and M100 (87.22%).

Histological Effect of Aspirin on the Stomach of Male Albino Swiss Mice (Mus musculus)

The present study aimed to determine the impacts of Aspirin drug on the stomach of albino Swiss mice. The study sample included 10 male mice divided into 2 groups. The first group was orally administered with 0.1 mL of 0.75 mg/kg aspirin once daily, whereas the second group, the control, was treated with similar doses of distilled water. Following 60 days of successive treatment, a number of parameters was studied including difference in body weight and histopathological changes in the stomach as diagnosed after histological preparation. The results showed a significant decrease (p<0.05) in body weight average of the treated mice compared with the control group. The results also revealed the occurrence of several histopathological changes in the stomach of treated animals, including raptures in the epithelium lining, congestion in the muscular layer, and vasodilation. In addition, alterations such as hemorrhage, pyknosis in muscle cells, sloughing of the lining of the muscular layer, and external bleeding were observed. It can be concluded from these results that orally administered aspirin exerts negative effects on the stomach in mice.

Mesoporous HBeta zeolite obtained via zeolitic dissolution–recrystallization successive treatment for vapor-phase Doebner–Von Miller reaction to quinolines

Mesoporous HBeta zeolite was obtained via zeolitic dissolution–recrystallization successive treatment, and exhibited enhanced selectivity to Q, anti-alkylation ability and stability in the vapor-phase Doebner–Von Miller reaction.

The role of the balneo-physical-kinetic treatment on the ligamentous stability in osteoarthritis of the knee

Osteoarthritis of the knee is one of the disabling conditions with a major impact on the quality of life of the patients. Existing therapies focus on reducing pain and disability caused by the disease. The existence of scores that evaluate the different aspects of patients quality of life are often used in monitoring the treatment and its results. The study is performed on a batch of 21 patients, between June 2017 and June 2018. They performed a complex rehabilitation program being evaluated every six months by applying the WOMAC questionnaire and performing specific maneuvers. The study has shown that by performing complex rehabilitation treatments the patients ability to perform some actions as well as their quality of life is improved and the effect following repeated treatments every six months is maintained during two treatments successive treatment.

Phenology of seed maturation in babysbreath (Gypsophila paniculata) in northwest Michigan, USA, and its relation to glyphosate efficacy

Babysbreath or perennial babysbreath (Gypsophila paniculata L.) is an aggressive invasive plant in large parts of southern Canada and the northern and western United States. It reproduces and disperses by seed, so the phenology of seed maturation is important in designing management programs. The present study provides the first quantitative assessment of G. paniculata seed-maturation phenology in a field population, as well as the first quantitative assessment of how the efficacy of herbicide treatment in preventing production of germinable seeds depends on the timing of treatment in relation to this phenology. Seeds were collected from untreated plants on five dates during July and August in both 2016 and 2017 and tested for germinability. Percent germination increased from 20% to 81% between July 22 and 28 and exceeded 90% by August 4, 2016. The seed-maturation phenology in 2017 was similar but delayed by about 4 d. On a growing degree-day scale, seed-maturation phenologies for the 2 yr were nearly identical. We also tested germinability of seeds from plants sprayed with glyphosate (23.4 ml ae L−1) on July 11, 18, and 25, 2016 (one date per plant). Percent germination increased from 0% to 13% to 20% over successive treatment dates, highlighting the importance of completing treatment early in the growing season.