scholarly journals Histological Effect of Aspirin on the Stomach of Male Albino Swiss Mice (Mus musculus)

2020 ◽  
pp. 1275-1281
Author(s):  
Zainab Karim Al-Timimi
Keyword(s):  
Body Weight ◽  
Control Group ◽  
Male Mice ◽  
Histopathological Changes ◽  
Negative Effects ◽  
Successive Treatment ◽  
Once Daily ◽  
Muscular Layer ◽  
Swiss Mice ◽  
Histological Effect

The present study aimed to determine the impacts of Aspirin drug on the stomach of albino Swiss mice. The study sample included 10 male mice divided into 2 groups. The first group was orally administered with 0.1 mL of 0.75 mg/kg aspirin once daily, whereas the second group, the control, was treated with similar doses of distilled water. Following 60 days of successive treatment, a number of parameters was studied including difference in body weight and histopathological changes in the  stomach as diagnosed after histological preparation. The results showed a significant decrease (p<0.05) in body weight average of the treated mice compared with the control group. The results also revealed the occurrence of several histopathological changes in the stomach   of treated animals, including raptures in the epithelium lining, congestion in the muscular layer, and vasodilation. In addition, alterations such as hemorrhage, pyknosis in muscle cells, sloughing of the lining of the muscular layer, and external bleeding were observed. It can be concluded from these results that orally administered aspirin exerts negative effects on the stomach in mice.

scholarly journals Study of the protective effect of Nigella sativa against Cimetidine induced reproductive toxicity in male mice

2010 ◽  
Vol 9 (1) ◽  
pp. 55 ◽  
Author(s):  
K. G. Ch. Al-Nailey
Keyword(s):  
Nigella Sativa ◽  
Aqueous Suspension ◽  
Reproductive Toxicity ◽  
Reproductive Organs ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Histopathological Changes ◽  
Once Daily ◽  
Long Term Treatment

The present study was designed to investigate some histopathological changes produced by cimetidine on male reproductive organs (testes & epididymis) in mice and extended to examine where there using of an aqueous suspension of powdered Nigella sativa seeds have the ability to reduce this toxicity. Forty male mice were divided into 4 groups. The first group received 25 mg/kg B.W. of cimetidine orally once daily. The second group drenched aqueous suspension of Nigella sativa at a dose of 1000mg/kg B.W. and then received cimetidine. While the third group drenched Nigella sativa aqueous suspension once daily, while the fourth group (control group) received normal saline only and the study was continued for (76 days). Animals which were treated with cimetidine only along the period of the experiment showed severe pathological changes, particularly in testes and epididymis, whereas oral administration of the aqueous suspension of Nigella seeds aqueous suspension significantly reduced these changes which reflected by an obvious increment of spermatogonia and reducing the damage of seminiferous tubules. The protective activity of Nigella sativa has been resumed via diminishing the reproductive histopathological changes associated with long-term treatment with cimetidine.

scholarly journals Effect of Oral Gavage Dosing Regimens in Female Tg rasH2 Transgenic Mice

2004 ◽  
Vol 32 (4) ◽  
pp. 413-417 ◽  
Author(s):  
Daniel Morton ◽  
Rani S. Sellers ◽  
Sylvia M. Furst ◽  
Kristen D. Hawley ◽  
Jeffrey R. May
Keyword(s):  
Body Weight ◽  
Incidental Finding ◽  
Sodium Dodecyl ◽  
Control Group ◽  
Oral Gavage ◽  
Once Daily ◽  
Dosing Regimens ◽  
Rash2 Mice ◽  
Daily Water ◽  
Cortical Architecture

Female Tg rasH2 (CB6F1/Jic-TgrasH2@Tac) mice were administered water once daily, water twice daily with 8 or 12 hours between doses, 1% sodium dodecyl sulfate in water (1% SDS) once daily, or 1% SDS twice daily with 12 hours between doses by oral gavage at volumes of 10 ml/kg/day for 28 or 29 consecutive days. A control group of mice received no treatment and no sham manipulation. There were no significant differences in body weight or food consumption between treated groups and untreated control mice. Mean weights of spleens, livers, and thymuses were lower than control values in most groups of mice subjected to gavage. Focal or multifocal loss of thymic cortical architecture was observed in 13 of 50 mice distributed among all groups (including naïve controls), however only in one instance was this finding suggestive of a precursor to neoplasia. This study demonstrated that Tg rasH2 mice can tolerate once or twice daily gavage dosing with water or vehicle containing 1% SDS. Loss of thymic cortical architecture was a common incidental finding in female Tg rasH2 mice.

scholarly journals Toxic Effects of Vitex Negundo L. And Polygonum Hydropiper L. Plant Extract Treated Fishes on Rats

2021 ◽  
Vol 49 (1) ◽  
pp. 125-135
Author(s):  
Molly Harun ◽  
Gulroo Begum Sufi ◽  
Gulshan Ara Latifa
Keyword(s):  
Body Weight ◽  
Radiation Dose ◽  
Plant Extracts ◽  
Control Group ◽  
Vitex Negundo ◽  
Histopathological Changes ◽  
Untreated Control Group ◽  
Polygonum Hydropiper ◽  
Treated Fish ◽  
Dried Fish

An observation was made to taste the toxic effect of Vitex negundo L. and Polygonum hydropiper L. (leaf and bark) plants extracts treated fish fed group on rats, Bandicota indica. Six group of rats were fed on two different kinds of plants extracts, one is radiation dose and another untreated control group. These were, the untreated control group rats were feed on normal food such as dried fish and wheat whereas treated rats were given plant extracts and radiation dose treated by dried fish. The experimental rats were fed of 4 ml dose of plant extracts in 70% ethyl alcohol (ratio 1:10) and 4 kGy radiation treated dry fish. The toxic properties of these plant extracts were carried out the vital organs of liver, kidney, stomach, spleens, lungs and muscles on male rats .The lower mammalian species rats were used for preliminary survey their body weight gained and histopathological changes were observed. The experiment was carried out from August 2004 to December 2004. Initially the concentration of rats body weights were found as, control group (C) 43 g/m, Vitex negundo leaf treated fish fed group of rat (VL) 44.8 g/m, Vitex negundo bark treated fish fed group of rat (VB) 44.4 g/m, Polygonum hydropiper leaf treated fish fed group of rat (PL) 46.2 g/m, Polygonum hydropiper bark treated fish fed group of rat (PB) 47.8 g/m and radiation treated fish fed group of rat (R) 47 g/m. After 30 days the weight gain of rats recorded as, group (C) 324.6 g/m, (VL) 357.2 g/m, (VB) 362 g/m, (PL) 342 g/m, (PB) 323.6 g/m and (R) 352 g/m and 90 days after the body weight were group (C) 727.2 g/m, (VL) 781.2 g/m, (VB) 795.8 g/m, (PL) 780.4 g/m, (PB)764.8 g/m and R 789.8 g/m respectively. The present study is, the first attempt to use the rats body weight data found well and most suitable 4ml dose of V. negundo bark treated extracts than leaf treated group and 4 kGy radiation group. Histopathological changes of rats organs test liver was well preserved, kidney-the glomeruli, tubules, blood vessel were showed no significant changes, in the stomach-the mucosa, sub mucosa were well, in the spleen-red and white pulp were showed no significant changes, lung-the alveoli was good and in the muscle-the skeletal muscle were remain unaltered. Bangladesh J. Zool. 49(1): 125-135, 2021

Berberis vulgaris L. effects on oxidative stress and liver injury in lead-intoxicated mice

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Jawhar Laamech ◽  
Jaouad El-Hilaly ◽  
Hamadi Fetoui ◽  
Yassine Chtourou ◽  
Hanane Gouitaa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Aqueous Extract ◽  
Lead Acetate ◽  
Protein Carbonyls ◽  
Control Group ◽  
Distilled Water ◽  
Once Daily ◽  
Group 2 ◽  
Mice Liver

AbstractBackgroundL. (BV), commonly known as “MethodsSixty IOPS mice were divided into six groups and were treated as follows: group 1 (normal control) received double distilled water; group 2 (toxic control) received lead acetate (5 mg/kg body weight/day) in double distilled water for 40 days; groups 3–6 received BV aqueous extract at doses of 25, 50, 100 and 150 mg/kg body weight , respectively, once daily for 30 days from 11 day after beginning of lead acetate exposure to the end of the experiment.ResultsToxic control group showed a significant alteration of serum alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), total cholesterol (TC), total bilirubin (TB), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH). Histological assessment of lead-intoxicated mice liver revealed alterations in hepatocytes and focal necrosis. BV treatment significantly prevented lead accumulation, increased ALT, AST, TC, and TB, inhibited lipid peroxidation and protein carbonyls(PCO) formation. Additionally, BV extract normalized the antioxidant enzymes (CAT, SOD and GPx), GSH and architecture of liver tissues.ConclusionsBV aqueous extract exerts significant hepatoprotective effects against lead-induced oxidative stress and liver dysfunction. The BV effect may be mediated through the enhancement of antioxidant status, lead-chelating abilities and free radicals quenching.

scholarly journals Curcumin Protects the Seminal Vesicles from Metronidazole‑induced Reduction of Secretion in Mice

2012 ◽  
Vol 55 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Ali Noorafshan ◽  
Saied Karbalay‑Doust
Keyword(s):  
Body Weight ◽  
Seminal Vesicle ◽  
Structural Changes ◽  
Reproductive Tract ◽  
Anaerobic Bacteria ◽  
Seminal Vesicles ◽  
Female Reproductive Tract ◽  
Sperm Chromatin ◽  
Control Group ◽  
Negative Effects

Seminal vesicle secretion is important for increasing the stability of sperm chromatin, inhibition of the immune activity in the female reproductive tract and so on. Metronidazole (MTZ), a drug used for treatment of infections caused by anaerobic bacteria and protozoa, may have negative effects on the genital gland including the seminal vesicles. Curcumin exhibits antioxidant as well as anti‑inflammatory properties. The present study aims to evaluate the negative effects of MTZ on the seminal vesicle structure and ameliorative effects of curcumin using stereological methods. Thirty balb/c mice were divided into six groups. The control group was received distilled water. The second and the third received higher doses of MTZ (500 mg/kg body weight/day) and MTZ (500 mg/kg/day) + 100 mg/kg/day curcumin, respectively. The fourth and the fifth were treated with lower doses of MTZ (165 mg/kg body weight/day) and MTZ (165 mg/kg body weight/day) + curcumin (100 mg/kg body weight/day), respectively. The sixth group received 100 mg/kg body weight/day curcumin. All the administrations were done by oral gavages for 14 days. After 30 days, seminal vesicles were removed. Stereological study of the seminal vesicle structure revealed a significant reduction in gland and vesicular fluid volume in MTZ‑treated (higher or lower doses) animals. Curcumin protected the reduction of both parameters in therapeutic‑dose treated animals. Metronidazole treatment does not induce structural changes in the seminal gland; however, it can have a significant impact on its secretion ability. Importantly, these deteriorations might be preventable by curcumin co‑treatment.

scholarly journals METRONIDAZOLE INDUCES SIGNIFICANT PATHOLOGICAL ALTERATIONS IN THE MALE REPRODUCTIVE SYSTEM OF MICE

2021 ◽  
Vol 52 (6) ◽  
pp. 1375-1381
Author(s):  
Z. K. Al – Timimi
Keyword(s):  
Reproductive System ◽  
Sperm Morphology ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Histopathological Changes ◽  
Negative Effects ◽  
The Mean ◽  
Water Treated Control

The present study was aimed to investigate the possible effects caused by the antibiotic, metronidazole, on the histology of the testis and the morphology of the sperms of mice. To achieve this,  adult male albino Swiss Mice were orally administrated with 0.1 ml (100 mg\ kg ) of the commercially used metronidazole (Flagyl) for 60 consecutive days. The results showed significant decreases (p<0.05) in the mean weight of body and testis in the treated mice as compared to the distilled water-treated control group. The treatment also caused several histopathological changes in the testis which included necrosis, congestion, hemorrhage, edema, germ cell downfall  and rupture in the seminiferous tubules. The tests of the sperm morphology revealed a number of deformations as a result of the treatment, such as the appearance of headless,  bend, broken-tailed, hook-tailed, and two-headed sperms, as well as sperms with cytoplasmic droplets. We conclude that from these results that orally administered Metronidazole exerts negative effects on the male reproductive system of mice .

scholarly journals Complementary effects of coenzyme Q10 and Lepidium sativum supplementation on the reproductive function of mice: An experimental study

2021 ◽  
Author(s):  
Fatemeh Rahimi Asl ◽  
Maryam Khosravi ◽  
Ramin Hajikhani ◽  
Jalal Solati ◽  
Hossein Fahimi
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Coenzyme Q10 ◽  
Follicle Stimulating Hormone ◽  
Reproductive Function ◽  
Serum Testosterone ◽  
Lepidium Sativum ◽  
Control Group ◽  
Male Mice ◽  
Weight Groups

Background: Coenzyme Q10 (CoQ10) and Lepidium sativum (LS) have therapeutic effects on infertility. Objective: To evaluate the combined effects of LS and CoQ10 on reproductive function in adult male NMRI mice. Materials and Methods: Eighty three-months-old male mice (35–40 gr) were divided into four groups (n = 10/each): control (treated with water), CoQ10-treated (200, 300, and 400 mg/kg/body weight), LS-treated (200, 400, 600 mg/kg/body weight), and co-treated (LS [600 mg/kg/body weight] + CoQ10 [200 mg/kg/body weight]) groups. Serum testosterone, luteinizing hormone, follicle-stimulating hormone, and gonadotropin realizing hormone (GnRH) levels were measured using ELISA method. The sperm quality was assessed using Sperm Class Analyzer® (SCA) CASA system and GnRH mRNA expression levels were evaluated by real-time polymerase chain reaction. Results: The number of sniffing and following behavior was significantly higher in LStreated (400 and 600 mg/ml/body weight) groups than the control group (p = 0.0007 and p = 0.0010, respectively). The number of mounting and coupling behaviors was significantly higher in the CoQ10 (300 and 400 mg/ml/body weight)-treated animals than the control group (p = 0.0170 and p = 0.0006, respectively). Co-treatment of CoQ10 (200 mg/ml/body weight) and LS (600 mg/ml/body weight) significantly increased all aspects of sexual behaviors as well as the levels of serum testosterone (p = 0.0011), luteinizing hormone (p = 0.0062), and follicle-stimulating hormone (p = 0.0001); sperm viability (p = 0.0300) and motility (p = 0.0010); and GnRH mRNA levels (p = 0.0016) compared to the control group. Conclusion: The coadministration of CoQ10 and LS significantly improves the activity of the hypothalamic-pituitary-gonadal axis and enhances the reproductive parameters in adult male mice. Key words: Lepidium sativum, Coenzyme Q10, Infertility, Male reproductive function.

Effect of Paracetamol on Sperm Biological Parameters and Testosterone Level in Albino Male Mice

2021 ◽  
pp. 51-57
Author(s):  
Habiba Eljaafari ◽  
Zainab EL Mabrouk ◽  
Marwan Rashrash
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Testosterone Level ◽  
Sperm Morphology ◽  
Sperm Parameters ◽  
Male Mice ◽  
Once Daily ◽  
High Doses ◽  
Fertilizing Capability ◽  
Different Doses

Backgrounds and objectives. The wide use of paracetamol at high doses was found to alter sperm parameters especially sperm morphology, and thus its fertilizing capability. Therefore, the present study was designed to use different doses of paracetamol to identify its effect on sperm parameters and testosterone levels in adult male mice. Methods. Forty adult male albino mice were divided into four equal groups, the first group injected with distilled water, the three treated groups injected with different doses of paracetamol (20, 40, 80 mg/kg body weight /day) over a period of 42 days. All doses were given once daily via intraperitoneal injection. Results. The results showed that paracetamol causes a significant decrease in body weight, non-significance effect on sperm parameters at doses of 20 and 40 mg/kg, while it led to a significant effect on sperm parameters at a dose of 80 mg/kg. Also, there was no difference in testosterone level between control and the treated groups (20 and 40mg/kg). But it showed a significant decrease in testosterone level at dose 80 mg/kg treated groups. Conclusion. It is considered safe to use paracetamol at doses 20 and 40 mg/kg but the dose 80 mg/kg has adverse effects on sperm parameters and testosterone level.

scholarly journals Susceptibility and morbidity between male and female Swiss mice infected with Angiostrongylus costaricensis: Susceptibilidade e morbidade entre camundongos Swiss machos e fêmeas infectados com Angiostrongylus costaricensis

2010 ◽  
Vol 52 (5) ◽  
pp. 267-268
Author(s):  
Márcia B. Mentz ◽  
Eliane Dallegrave ◽  
Carlos Graeff-Teixeira
Keyword(s):  
Body Weight ◽  
Parasitic Infections ◽  
Male Mice ◽  
Male And Female ◽  
Swiss Mice ◽  
Intestinal Lesions ◽  
Vertebrate Hosts

The gender of vertebrate hosts may affect the outcome of parasitic infections. An experimental murine infection with Angiostrongylus costaricensis was followed with determinations of body weight, fecal larval elimination, number and length of adult worms, number of macroscopic intestinal lesions, and mortality. Groups of male and female Swiss mice were infected with 10 3rd-stage A. costaricensis larvae per animal. The results indicate there are no significant differences related to gender of the host, except for higher length of worms developed in male mice.

scholarly journals The Probiotic Lactobacillus fermentum Biocenol CCM 7514 Moderates Campylobacter jejuni-Induced Body Weight Impairment by Improving Gut Morphometry and Regulating Cecal Cytokine Abundance in Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 235
Author(s):  
Miroslava Anna Šefcová ◽  
Marco Larrea-Álvarez ◽  
César Marcelo Larrea-Álvarez ◽  
Viera Karaffová ◽  
David Ortega-Paredes ◽  
...  
Keyword(s):  
Body Weight ◽  
Campylobacter Jejuni ◽  
Cytokine Response ◽  
Lactobacillus Fermentum ◽  
Control Group ◽  
Negative Effects ◽  
Effective Response ◽  
Probiotic Lactobacillus ◽  
Intestinal Morphometry ◽  
The Impact

This research was conducted to investigate if the administration of the probiotic Lactobacillus fermentum could influence body weight, intestinal morphometry and the cecal cytokine response in Campylobacter jejuni-infected chickens. Seventy-two 1-day old COBB 500 male chicks were allocated randomly into four experimental groups. (I) Control group (C), in which chicks were left untreated. (II) LB group, treated with L. fermentum. (III) Cj group, infected with C. jejuni and (IV) coexposure group in which both bacteria were administered. Body weight was registered and then all birds were slaughtered; samples from the small intestine and caecum were collected at 4- and 7-days post infection. The experiment lasted eleven days. Villi height and crypt depth ratios of the duodenum, jejunum and ileum were evaluated using appropriate software, while reverse transcription quantitative PCR (RT-qPCR) was utilized for assessing transcript levels of key cecal inflammatory cytokines (IL-1β, IL-18, IL-17, IL-15, IL13 and IL-4). Campylobacter-infected birds showed lower body weight values than those supplemented with the probiotic; these birds, in turn, proved to be heavier than those reared under control conditions. L. fermentum administration improved morphometrical parameters of the duodenum, jejunum and ileum; in general, villi were larger and crypts deeper than those identified in control conditions. Moreover, the negative effects elicited by C. jejuni were not observed in chickens exposed to the probiotic. Significant differences were also determined with regards to transcript abundance of all evaluated cytokines in the caecum. C. jejuni induced a downregulation of the studied interleukins; however, such a response was heightened by administration of L. fermentum, with an increase rate of transcription that promoted a more effective response to a C. jejuni infection. The effects of experimental treatments proved to vary between sampling points. Conclusively, these results demonstrate that L. fermentum lessens the negative effects elicited by C. jejuni on body weight by alleviating the impact on intestinal morphometry and cecal cytokine response, which ultimately improve chicken growth performance.

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