GC-MS Analysis and Molecular Docking of Bioactive Components from Leaves of Guaiacum officinale for Anti-inflammatory Activity

Present study was an attempt to investigate the bioactive components present in the leaves of Guaiacum officinale using gas chromatography-mass spectroscopy (GC-MS) analysis and study the antiinflammatory potential of those constituents using molecular docking studies. GC-MS analysis was done by standard protocol using the equipment JEOL GC MATE II. The identification of components was based on NIST (National Institute of Standards and Technology) Version-11 library as well as comparison of their retention indices. The molecular docking studies were done using the commercial docking software MCULE, 1-click docking. GC-MS analysis of the alcoholic extract showed the presence of ten compounds at different retention times. The phytoconstituent 8,11,14-eicosatrienoic acid was present at high concentration with % peak area of 43.3 at a retention time of 19.43 min followed by 5,7-dihydroxy-8-methoxy flavone (Wogonin) at a retention time of 17.73. All 10 compounds obtained from GC-MS analysis and diclofenac were used as the ligands in this study, with cyclooxygenase-2 (COX-2), phospholipase A2 and interleukin receptor as the molecular targets. in silico Docking studies revealed that the flavanoid Wogonin is having highest binding potential indicated by least docking score of -8.2, -8 and -6.9 kcal/mol on COX-2, phospholipase A2 and interleukin receptor respectively.

ANTIDIABETIC EFFECT OF GUAIACUM OFFICINALE

Introduction: Diabetes is the most genetically transmitted disease all over theworld. It badly affects the vital organs and manifest the reduction of pancreatic amylase andlipase. Objectives: This study has been designed to investigate the role of Guaiacum officinaleextract in controlling of diabetes in streptozotocin (STZ) induced type 2 diabetes male albino(Wistar) rats. Study Design: Experimental. Setting: Baqai Medical University. Period: January2017- June 2017. Method: Bark extract of Guaiacum officinale (500mg/kg) was administeredto STZ induced rat. Glibenclamide (GLB) was used as standard drug. The approach of thestudy was to observe the effect of Guaiacum officinale on pancreatic amylase and lipase and itstissue architecture by histopathology. Rats were divided in four groups i-e control, STZ treated,STZ + GLB treated and STZ +extract treated group. Results: Guaiacum officinale significantlyimprove the level of pancreatic amylase and lipase as compared to the STZ induced group.Similarly the pancreatic architecture was significantly affected by STZ alone. These changeswere considerably reversed by Guaiacum officinale and GLB. Conclusion: It was concludedthat Guaiacum officinale herb useful to cure the diabetes and it is effective in protecting pancreasfrom diabetes induced damages.

Variación interanual de la floración y visita de abejas en cuatro especies de árboles de bosque seco en República Dominicana

Entre septiembre 2010 a agosto 2013, en los municipios Bánica y Pedro Santana, en el suroeste de la República Dominicana, se realizaron observaciones de la floración de cuatro especies de interés apícola presentes en el bosque seco de la República Dominicana: Guaiacum officinale, Bursera simaruba, Acacia scleroxylon y Zizyphus rignonii. Durante los tres años, el momento de inicio de la floración no varió o solamente varió en una o dos quincenas. La proporción de árboles que llegaron a florecer disminuyó sensiblemente en los 2012 y 2013, período con época seca muy intensa. La intensidad de la visita de abejas en las flores también disminuyó en ese período. Además, la duración de la floración de Bursera simaruba y Acacia scleroxylon fue afectada negativamente por un exceso de lluvia de marzo a mayo 2012. Como recomendación de amortiguamiento al cambio climático, se recomienda mantener una diversidad de especies vegetales de interés apícola con diferentes épocas de floración.