Tenebrio molitor in the circular economy: a novel approach for plastic valorisation and PHA biological recovery

The increase in the world population leads to rising demand and consumption of plastic raw materials; only a small percentage of plastics is recovered and recycled, increasing the quantity of waste released into the environment and losing its economic value. The plastics represent a great opportunity in the circular perspective of their reuse and recycling. Research is moving, on the one hand, to implement sustainable systems for plastic waste management and on the other to find new non-fossil-based plastics such as polyhydroxyalkanoates (PHAs). In this review, we focus our attention on Tenebrio molitor (TM) as a valuable solution for plastic biodegradation and biological recovery of new biopolymers (e.g. PHA) from plastic-producing microorganisms, exploiting its highly diversified gut microbiota. TM’s use for plastic pollution management is controversial. However, TM microbiota is recognised as a source of plastic-degrading microorganisms. TM-based plastic degradation is improved by co-feeding with food loss and waste as a dietary energy source, thus valorising these low-value substrates in a circular economy perspective. TM as a bioreactor is a valid alternative to traditional PHA recovery systems with the advantage of obtaining, in addition to highly pure PHA, protein biomass and rearing waste from which to produce fertilisers, chitin/chitosan, biochar and biodiesel. Finally, we describe the critical aspects of these TM-based approaches, mainly related to TM mass production, eventual food safety problems, possible release of microplastics and lack of dedicated legislation.

Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection

Electrostatic samplers have been increasingly studied for sampling of viral and bacterial aerosols, and bioaerosol samplers are required to provide concentrated liquid samples with high physical collection and biological recovery, which would be critical for rapid detection. Here, the effects of sampling media and protocols on the physical collection and biological recovery of two airborne bacteria (Pseudomonas fluorescens and Micrococcus luteus) under electrostatic field were investigated using a personal electrostatic particle concentrator (EPC). Deionized (DI) water with/without sodium dodecyl sulfate (SDS) and phosphate buffered saline were tested as sampling media. A polystyrene container was mounted onto the collection electrode of the EPC for stable storage and vortexing after capture. Many bacterial cells were found to be deposited on the bottom surface of the container submerged in the media via electrophoresis, and among the tested sampling protocols, wet sampling with a container and subsequent vortexing offered the most bacteria in the collection suspension. Experiments with several sampling media showed that 0.001–0.01% SDS-DI water demonstrated the highest recovery rate in the EPC. These findings would be valuable in the field of sampling and subsequent rapid detection of bioaerosols.

Negative resistance and resilience: biotic mechanisms underpin delayed biological recovery in stream restoration

Traditionally, resistance and resilience are associated with good ecological health, often underpinning restoration goals. However, degraded ecosystems can also be highly resistant and resilient, making restoration difficult: degraded communities often become dominated by hyper-tolerant species, preventing recolonization and resulting in low biodiversity and poor ecosystem function. Using streams as a model, we undertook a mesocosm experiment to test if degraded community presence hindered biological recovery. We established 12 mesocosms, simulating physically healthy streams. Degraded invertebrate communities were established in half, mimicking the post-restoration scenario of physical recovery without biological recovery. We then introduced a healthy colonist community to all mesocosms, testing if degraded community presence influenced healthy community establishment. Colonists established less readily in degraded community mesocosms, with larger decreases in abundance of sensitive taxa, likely driven by biotic interactions rather than abiotic constraints. Resource depletion by the degraded community likely increased competition, driving priority effects. Colonists left by drifting, but also by accelerating development, reducing time to emergence but sacrificing larger body size. Since degraded community presence prevented colonist establishment, our experiment suggests successful restoration must address both abiotic and biotic factors, especially those that reinforce the ‘negative’ resistance and resilience which perpetuate degraded communities and are typically overlooked.

Physical Collection and Viability of Airborne Bacteria Collected under Electrostatic Field with Different Sampling Media and Protocols Towards Rapid Detection

Electrostatic samplers have been increasingly studied for sampling of viral and bacterial aerosols, and bioaerosol samplers are required to provide concentrated liquid samples with high physical collection and biological recovery, which would be critical for rapid detection. Here, the effects of sampling media and protocols on the physical collection and biological recovery of two airborne bacteria (Pseudomonas fluorescens and Micrococcus luteus) under electrostatic field were investigated using a personal electrostatic particle concentrator (EPC). Deionized (DI) water with/without sodium dodecyl sulfate (SDS) and phosphate buffered saline were tested as sampling media. A polystyrene container was mounted onto the collection electrode of the EPC for stable storage and vortexing after capture. Many bacterial cells were found to be deposited on the bottom surface of the container submerged in the media via electrophoresis, and among the tested sampling protocols, wet sampling with a container and subsequent vortexing offered the most bacteria in the collection suspension. Experiments with several sampling media showed that 0.01% SDS-DI water demonstrated a higher recovery rate and larger total bacterial concentrations in the EPC than in the BioSampler. These findings would be valuable in the field of sampling and subsequent rapid detection of bioaerosols.

Recovery of acidified Sudbury, Ontario, Canada, lakes: a multi-decade synthesis and update

The Sudbury region of northeastern Ontario, Canada, provides one of the world’s best examples of the resilience of aquatic ecosystems after reductions in atmospheric contaminant deposition. Thousands of lakes around the Sudbury metal smelters were badly damaged by acid deposition. Lakes closest to the smelters were also contaminated by metal particulates. However, large reductions in atmospheric SO2 and metal emissions starting in the early 1970s have led to widespread chemical improvements in these lakes, and recovery has been observed for various aquatic biota. Studies of Sudbury-area lakes are advancing our understanding of chemical and biological lake recovery; however, recovery is a complicated process and much remains to be learned. Biological recovery has often been slow to follow chemical recovery, and it has become apparent that the recovery of lakes from acidification is closely linked to interactions with other large-scale environmental stressors like climate change and Ca declines. Thus, in our multiple-stressor world, recovery may not bring individual lakes back to their exact former state. However, with time, substantial natural biological recovery toward typical lake communities can be reasonably expected for most but not necessarily all biota. For organisms with limited dispersal ability, particularly fish, human assistance may be necessary to re-establish typical communities. In lakes where food webs have been severely altered, re-establishment of typical diverse fish communities may in fact be an important element aiding the recovery of other important components of aquatic ecosystems including zooplankton and benthic macroinvertebrates. In the lakes closest to the smelters, where historically watersheds as well as lakes were severely damaged, the recovery of aquatic systems will be closely linked to ongoing terrestrial recovery and rehabilitation, particularly through the benefits of increased inputs of terrestrially derived organic matter. The dramatic lake recovery observed in the Sudbury area points to a brighter future for these lakes. However, continued monitoring will be needed to determine future changes and help guide the management and protection of Sudbury-area lakes in this multiple-stressor age.