Abstract
Background The aim of our study was to observe the quantitative changes in tibial plateau chondrocytes in the proliferation process from normal Gottingen pigs in vitro and compare them with the Fibonacci sequence.Methods Chondrocytes from normal Gottingen pig tibial plateau cartilage were isolated and cultured to the third generation, and the number of chondrocytes was measured to determine whether the chondrocyte growth was at the logarithmic growth phase. Chondrocytes were added to cell culture bottles at very a low density to allow full separation and allow each chondrocyte to grow as independently as possible. Thirty single chondrocytes were selected, and the number of chondrocyte colonies were observed and recorded every day. Results Among the 30 chondrocyte colonies, the changes in the numbers of 16 chondrocyte colonies were 1, 2, 3, 5, 8, 13, 21, 34, 55, 89, 144, 233, 377, 610, and 987, which conformed to the numbers of the Fibonacci sequence.The number of other chondrocyte colonies was lower than corresponding numbers of the Fibonacci sequence at the same time point.Conclusion The numbers of normal Gottingen pig tibial plateau chondrocytes during the proliferation process were in line with the numbers of the Fibonacci sequence.Alignment to the Fibonacci sequence may be the fastest way for changes in the numbers of normal chondrocytes during the proliferation process in vitro.