The concept of biaxial surface potential and polymer aligning layers in electro-optical cells based on helical nanostructures of ferroelectric liquid crystal

The paper considers the possibility of controlling the alignment quality of helical nanostructures of ferroelectric liquid crystals (FLCs) within the concept of biaxial surface potential due to variation the FLCs helical pitch p0 and polymer aligning layers structures.

Optical rotation dispersion of cholesteric-nematic mixture

The dispersion of the specific rotation constant of a cholesteric-nematic mixture of 5CB and Ch17 at a low concentration was studied by optical spectroscopy. The dispersion of the anisotropy of the refractive index as a function of the wavelength is studied, and the helical pitch of the cholesteric-nematic mixture is calculated.

CryoEM structure of the super-constricted two-start dynamin 1 filament

Dynamin belongs to the large GTPase superfamily, and mediates the fission of vesicles during endocytosis. Dynamin molecules are recruited to the neck of budding vesicles to assemble into a helical collar and to constrict the underlying membrane. Two helical forms were observed: the one-start helix in the constricted state and the two-start helix in the super-constricted state. Here we report the cryoEM structure of a super-constricted two-start dynamin 1 filament at 3.74 Å resolution. The two strands are joined by the conserved GTPase dimeric interface. In comparison with the one-start structure, a rotation around Hinge 1 is observed, essential for communicating the chemical power of the GTPase domain and the mechanical force of the Stalk and PH domain onto the underlying membrane. The Stalk interfaces are well conserved and serve as fulcrums for adapting to changing curvatures. Relative to one-start, small rotations per interface accumulate to bring a drastic change in the helical pitch. Elasticity theory rationalizes the diversity of dynamin helical symmetries and suggests corresponding functional significance.

Supramolecular fibrous gels with helical pitch tunable by polarity of alcohol solvents

The pitch of supramolecular helical fibers formed from palmitoylated 1,5-anhydro-d-glucitol was able to be tuned by controlling the polarity of alcohol solvent.

Models for predicting the performance of fertilizer metering in seed cum fertilizer drill

ABSTRACT This study aimed to establish mathematical models that predict the performance of helical fertilizer metering according to the longitudinal and transverse inclinations, angular speed, and helical pitch. Laboratory tests were carried out with helical meterings with lateral and longitudinal discharge through overflow and with two helicoids, working at an angular speed of 16 and 46 rpm at the following longitudinal inclination angles: -20, -15, -10, -5, 0, +5, +10, +15, and +20° and on the transverse axis: -15, -10, -5, 0, +5, +10, and +15°. It was found that the variation of the transverse inclination has little effect on the dosage. When using fertilizer metering with a helicoid, the higher the longitudinal inclination, the greater the dosage in a linear manner, while the meterings with two helicoids provided less oscillation of the dosages according to the inclinations.

The size of helical pitch is important for microtubule plus end dynamic instability

ABSTRACTMicrotubule (MT) dynamic instability is a conserved phenomenon underlying essential cellular functions such as cell division, cell migration and intracellular transport, and is a key target of many chemotherapeutic agents. However, it remains unclear how the organization of tubulin dimers at the nanometer scale translates into dynamic instability as an emergent property at the micrometer scale. Tubulin dimers are organized into left-handed helical MT lattice, and most present-day MTs converge at a 1.5 dimer helical pitch that causes a seam in an otherwise symmetric helix. Because presently there are no experimental methods that can precisely manipulate tubulin subunit with sub-dimer resolution, the impact of helical pitch on dynamic instability remains unknown. Here by using stochastic simulations of microtubule assembly dynamics we demonstrate that helical pitch plays essential roles in MT plus end dynamic instability. By systematically altering helical pitch size, one half-dimer at a time, we found that a helical pitch as small as one half-dimer is sufficient to inhibit short-term MT length plateaus associated with diminishing GTP-tubulin cap. Notably, MT plus end dynamics quantitatively scale with the size of helical pitch, rather than being clustered by the presence or absence of helical symmetry. Microtubules with a 1.5 dimer helical pitch exhibit growth and shrinkage phases and undergo catastrophe and rescue similar to experimentally observed microtubules. Reducing helical pitch to 0 promotes rapid disassembly, while increasing it causes microtubules to undergo persistent growth, and it is the 1.5 dimer helical pitch that yields the highest percentage of MTs that undergo alternating growth and shrinkage without being totally disassembled. Finally, although the 1.5 dimer helical pitch is conserved among most present-day MTs, we find that other parameters, such as GTP hydrolysis rate, can partially compensate for changes in helical pitch. Together our results indicate that helical pitch is a determinant of MT plus end dynamic instability and that the evolutionarily conserved 1.5 dimer helical pitch promotes dynamic instability required for microtubule-dependent cellular functions.