Influence of channel to heater width ratio on flow boiling critical heat flux in mini- and microchannels

Flow boiling experiments were conducted in mini- and microchannel with different ratio of heater width to a channel width. Decrease in critical heat flux is observed in transition from wide channel to channel with width equal to a heater width. Particularities of boiling phenomena in these conditions were observed by means of high-speed visualization, which provided an explanation to heat flux reduction.

Study of flow over piano key weir of different plan shapes with free and partially submerged outlet conditions

Piano key weirs are being increasingly used for better flood passage downstream, both as a new structure or on top of hydraulic structures like a dam, to increase their discharging capacity as well as reservoir storage. Much research has been done on rectangular plan-form while other plan-forms warrants attention. The present study focuses on two different plan geometries of PKW, i.e., rectangular (RPKW) and trapezoidal with angle α equal to 9 degrees (TPKW9) for their head discharge relation in a wide channel of 0.984 m width under free-flow condition. Since the role of CFD is increasingly becoming prominent in present times, a numerical study using ANSYS-FLUENT was also carried out to ascertain its relevance in predicting flows around complex structures like PKW. Further, the tailgate was closed to render the PKW's outlet from partial to fully submerged conditions. The effect of these submerged outlets was studied for any changes in the discharging capacity of the PKW. The study shows RPKW to be hydraulically efficient than TPKW9 for the model geometry. Furthur the study finds that under partial to full submergence of PKW outlets, both PKW units' discharging capability remains unchanged.

Biophysical Informatics Approach For Quantifying Phenotypic Heterogeneity In Cancer Cell Migration In Confined Microenvironments

Motivation Cancer cell heterogeneity can manifest genetically and phenotypically. Bioinformatics methods have been used to analyze complex genomics and transcriptomics data, but have not been well-established for analyzing biophysical data of phenotypically heterogeneous tumor cells. Here, we take an informatics approach to analyze the biophysical data of MDA-MB-231 cells, a widely used breast cancer cell line, during their spontaneous migration through confined environments. Experimentally, we vary the constriction microchannel geometries (wide channel, short constriction, and long constriction) and apply drug treatments. We find that cells in the short constriction are similar in morphology to the cells in the wide channel. However, their fluorescence profiles are comparable to those in the long constriction. We demonstrate that the cell migratory phenotype is correlated more to mitochondria in a non-confined environment and more to actin in a confined environment. We demonstrate that the cells' migratory phenotypes are altered by ciliobrevin D, a dynein inhibitor, in both confined and non-confined environments. Overall, our approach elucidates phenotypic heterogeneity in cancer cells under confined microenvironments at single-cell resolution. Results Here, we apply a bioinformatics approach to a single cell invasion assay. We demonstrate that this method can determine distinctions in morphology, cytoskeletal activities, and mitochondrial activities under various geometric constraints and for cells of different speeds. Our approach can be readily adapted to various heterogeneity studies for different types of input biophysical data. In addition, this approach can be applied to studies related to biophysical changes due to differences in external stimuli, such as treatment effects on cellular and subcellular activities, at single-cell resolution. Finally, as similar bioinformatics methods have been widely applied in studies of genetic heterogeneity, biophysical information extracted using this approach can be analyzed together with the genetic data to relate genetic and phenotypic heterogeneity. Availability The data that support the findings of this study are available from the corresponding author upon reasonable request. Supplementary information Supplementary data are available at Bioinformatics online.