Standard Practice for Preparation Of Cell Monolayers on Glass Surfaces for Evaluation of Microbicidal Properties of Non-Chemical Based Antimicrobial Treatment Technologies

10.1520/e3286 ◽  
2021 ◽  
Author(s):  
Keyword(s):  
Antimicrobial Treatment ◽  
Standard Practice ◽  
Cell Monolayers ◽  
Treatment Technologies ◽  
Glass Surfaces

In Situ Embedding of Cell Monolayers on Untreated Glass Surfaces for Vertical and Horizontal Ultramicrotomy.

1980 ◽  
Vol 38 ◽  
pp. 644-645
Author(s):  
Kai Chien
Keyword(s):  
Organic Solvent ◽  
Light Microscopy ◽  
Cell Monolayer ◽  
Glass Slide ◽  
Plastic Substrates ◽  
Cell Monolayers ◽  
Glass Surfaces ◽  
In Situ Embedding ◽  
The Ideal

Untreated glass is the ideal supporting substrate for cell cul¬ture growth since it is extremely flat and smooth, transparent and insoluable in organic solvent. However, difficulties have been encountered in removing polymerized epoxy resin from a glass surface following in situ cell monolayer embedment. Vari¬ous techniques have been made to grow cell cultures on either coated glass surfaces or plastic substrates. The purpose of this abstract is to describe a heat separation technique which when used together with a newly designed embedding mold allows cell monolayers to be transferred from untreated coverglass or glass slide to pre-shaped tissue blocks. The resulting tissue block can be easily separated and used directly for orientation light microscopy prior to ultramicrotomy.

scholarly journals Treponema pallidum receptor binding proteins interact with fibronectin.

1983 ◽  
Vol 157 (6) ◽  
pp. 1958-1970 ◽  
Author(s):  
K M Peterson ◽  
J B Baseman ◽  
J F Alderete
Keyword(s):  
Receptor Binding ◽  
Plasma Proteins ◽  
Binding Proteins ◽  
Treponema Pallidum ◽  
Surface Proteins ◽  
Host Cells ◽  
Oriented Attachment ◽  
Cell Monolayers ◽  
Glass Surfaces ◽  
Mechanism Of Recognition

Analysis of plasma proteins avidly bound to T. pallidum surfaces revealed the ability of T. pallidum to acquire numerous host macromolecules. No acquisition was evident by the avirulent spirochete, T. phagedenis biotype Reiter. Western blotting technology using hyperimmune antifibronectin serum as a probe revealed the ability of virulent treponemes to avidly bind fibronectin from a complex medium such as plasma. The specificity of the tiplike adherence of motile T. pallidum to fibronectin-coated glass surfaces and to fibronectin on HEp-2 cells was reinforced by the observation that pretreatment of coverslips or cell monolayers with monospecific antiserum against fibronectin substantially reduced T. pallidum attachment. The stoichiometric binding of T. pallidum to fibronectin-coated coverslips and the inability of unlabeled or 35S-radiolabeled treponemes to interact with glass surfaces treated with other plasma proteins further established the specific nature of the interaction between virulent T. pallidum and fibronectin. The avid association between three outer envelope proteins of T. pallidum and fibronectin was also demonstrated. These treponemal surface proteins have been previously identified as putative receptor-binding proteins responsible for T. pallidum parasitism of host cells. The data suggest that surface fibronectin mediates tip-oriented attachment of T. pallidum to host cells via a receptor-ligand mechanism of recognition.

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Transport in Caco-2 cell monolayers of cucurbitane triterpenoids from Momordica charantia

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
SB Wu ◽  
GGL Yue ◽  
AC Keller ◽  
MH To ◽  
CBS Lau ◽  
...  
Keyword(s):  
Momordica Charantia ◽  
Cell Monolayers

Acquired Factor XI Inhibitors in Two Patients with Hereditary Factor XI Deficiency

1984 ◽  
Vol 51 (03) ◽  
pp. 371-375 ◽  
Author(s):  
Kangathevy Morgan ◽  
Sandra Schiffman ◽  
Donald Feinstein
Keyword(s):  
Protein A ◽  
Lambda Light Chain ◽  
Factor Xi ◽  
Spontaneous Bleeding ◽  
Factor Xi Deficiency ◽  
Hereditary Factor ◽  
Coagulation Mechanism ◽  
Factor Xia ◽  
Polyclonal Igg ◽  
Glass Surfaces

SummaryTwo patients with hereditary factor XI deficiency developed inhibitors following plasma transfusions. Neither had severe spontaneous bleeding. The patients’ plasmas neutralized both factor XI in plasma, purified factor XI, and purified factor XIa. The inhibitor in both patients’ plasmas adsorbed to Protein A- Sepharose. The inhibitors eluted from Protein A-Sepharose were partially neutralized by kappa and lambda light chain antisera indicating that they were polyclonal IgG antibodies. Both inhibitors markedly decreased adsorption of factor XI to glass surfaces. The cleavage of factor XI by trypsin was unaffected by the inhibitors. The lack of severe spontaneous bleeding in both of these patients strongly suggests that an alternate coagulation mechanism bypassing factor XI must compensate for this severe defect.

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