High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma

Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be closely related to the recurrence of CSCs. However, whether dysregulations of miRNAs exist in MFH, CSCs remained unknown.Methods: In this study, miRNAs in MFH CSCs and MFH common cells were examined by gene probe. Then, target genes and their functions involved in the signal pathway were predicted by the relevant database. Finally, the miRNAs’ target regulatory network was constructed. Furthermore, the miRNAs and target genes were identified by quantitative polymerase chain reaction, whereas miRNA analogs and antagonists were transfected in tumor cells to investigate cell proliferation ability further.Results: Results showed that a total of 47 miRNAs were found, including 16 that were upregulated and 31 that were downregulated. The screened differential miRNA showed a different expression in the cell resistant strains compared with the control group. Quantitative polymerase chain reaction analysis confirmed that the relative abundance of seven miRNAs and four target genes varied significantly. The encouraging issue is that we found Hsa-miR-206 significantly inhibited MFH proliferative activity.Conclusion: Hsa-miR-206 played a key role in regulating MFH CSC properties that might be a representative marker and target for the diagnosis and treatment of MFH in the future.

Benign Fibrous Histiocytoma of the Sacrum: A Rare Scenario

Benign fibrous histiocytoma (BFH) of bone is quite rare, and here we report the second case of such tumor originating from sacrum, with the first being reported in an 18-year-old female. The overlapping clinical, radiological, and histopathological findings make it a difficult diagnosis along with the fact that it is a rare diagnosis. In this case report, we present the diagnostic difficulties and optimal treatment for such cases. A 46-year-old male w presented to OPD with complaint of numbness in left foot since 1 month, and intermittent urinary incontinence for 2 weeks. On examination, the straight leg raising test was positive of left side, extensor hallucis longus (EHL) was weak in both lower limbs, and bilateral ankle jerks were diminished. MRI showed well-defined lobulated solid mass lesion, which is T1 hypointense and T2 hyperintense and homogeneously involving the S1 vertebral body, with involvement of the right sacral ala, and right sacral foramen seen at S1 and S2 levels extending into the spinal canal till L4 level. Excision through a posterior midline incision was performed taking care to preserve the traversing nerves. Postoperatively, remarkable improvement in pain with no major residual neurological deficit was observed. Patient was followed-up till 9 months; patient’s incontinence improved over a period of 3 months and has stayed the same until the last follow-up.

Hyperpigmented Nodular Dermatofibroma: Two Cases Report and Brief Literature Review

BACKGROUND: Dermatofibroma (DF) is a common benign skin tumor (Benign Fibrous Histiocytoma) that mostly affects the extremities with a tendency to occur more often in older females than males. It usually presents as a slow growing small brown dome shape papule on the extremities. DF has a chronic nature and can sometimes regresses spontaneously. Dermoscopy is essential in the evaluation of DF to help differentiate it with other skin tumors. The gold standard evaluation for diagnosis of DF is biopsy with histopathologic examination. Removal of DF is often due to cosmetic factors, with surgical excision being the preferred method for removal. DF has an excellent prognosis. CASE REPORT: We present two case reports of women with hyperpigmented nodules on the lower extremity. Dimple sign was positive. From dermoscopic study showed a pigment network and central white patch pattern. On histologic examination revealed proliferation of fibroblast such as spindle cells as a storiform pattern and hyperplastic epidermis with hyperpigmentation of the basal layer. Based on clinical features, dermoscopy and histopathological evaluation, the diagnosis of DF was established. Both patients were perform surgically excision and have a good result. CONCLUSION: Dermatofibroma is benign fibrous histiocytoma that represents one of the most common skin tumours. Nodular hyperpigmented dermatofibroma is a clinical variant of Dermatofibroma which can be treated with surgical excision with good prognosis.

Benign fibrous histiocytoma of the femur: A rare pediatric case report

BACKGROUND: Benign fibrous histiocytoma is known to be a frequent skin tumor but its occurrence in bone remains very rare especially in pediatric population. This entity is a subject of interest also because histologically it can mimic other fibrohistiocytic lesions of bone such as non-ossifying fibroma. CLINICAL CASE: An 11-year-old patient admitted with swelling of the right thigh and intermittent pain. Radiological evaluation shows cystic lesion of the femur with a soap-bubble and a border of condensation. Histopathological examination of the biopsy sample established the diagnosis of benign fibrous histiocytoma. The patient underwent complete curettage of the lesion with bone graft. There is no recurrence 16 months after surgery. DISCUSSION: Benign fibrous histiocytoma is a rare bone tumor especially in children. Histologically it can mimic non-ossifying fibroma. Thus clinical and radiological features are important to differentiate these tumors in order to choose adequate treatment. CONCLUSIONS: Benign fibrous histiocytoma is probably underestimated in pediatric population. This diagnosis should be considered in any child or teenager who presents with a non-ossifying fibroma accompanied by unexplainable pain or a rapid growing.

Scapular Angiomatoid Fibrous Histiocytoma with EWSR1-CREB1 Fusion in an Adult Patient

Background. Angiomatoid fibrous histiocytoma (AFH) is a rare intermediate malignant tumor that arises mainly in soft tissues, especially in the superficial extremities of patients younger than 30 years. There have been a few reports of AFH arising from sites other than soft tissue, including bone, and unusual site and age make it difficult to diagnose this rare tumor. Case Presentation. Here, we present a case of a 54-year-old man who was examined for chest pain, and computed tomography (CT) incidentally detected a bone tumor at the scapula with destruction of cortical bone and invasion into soft tissue. Magnetic resonance imaging revealed multiple cystic components with fluid-fluid levels. FDG-PET showed uptake at the axillary lymph node. The CT-guided needle biopsy revealed spindle cell sarcoma on histopathology. After neoadjuvant chemotherapy, a scapulectomy was performed. The final postresection histopathological diagnosis was the same as the preoperative diagnosis, and no obvious chemotherapeutic effect was observed. Next-generation sequencing of RNA isolated from paraffin-embedded tumor tissue revealed that these lesions harbored the EWSR1-CREB1 fusion gene, and the tumor was diagnosed as AFH. C-reactive protein level, which was elevated preoperatively, decreased after the operation, and there was no recurrence or metastasis 5 years after the treatment. Conclusion. The diagnosis of AFH is difficult when the tumor has an atypical presentation. Comprehensive genomic analysis, especially RNA sequencing, is efficient in diagnosing this rare tumor. Moreover, magnetic resonance imaging findings identical to AFH in soft tissue, the presence of paraneoplastic symptoms such as elevated inflammatory markers, and lymph node swelling were clues towards suspecting this tumor.

Fibrous Histiocytoma of Anterior Ethmoidal Sinus: Case Report and Review of the Literature

Benign fibrous histiocytoma of the para nasal sinus is a rare tumor of this region which originates from mesenchymal cells. A 9-year-old girl presented with gradually enlarging mass above the medial canthus over 3 months. Orbital computerized tomography scan showed a round well-differentiated homogeneous mass in the anterior ethmoidal sinus. Histology and immunohistochemistry analysis after excision revealed proliferation of spindle-shaped fibroblasts in storiform pattern and histiocytes without mitosis and anaplasia. After 9 months from surgical excision, recurrence occurred which resulted in total excision again. This is the first reported case of benign fibrous histiocytoma involving the ethmoid sinus in Iranian people. Rare sinus tumor should be considered in the differential diagnosis of sinus tumors.