Impaired In-vitro Platelet Aggregation and Secretion Tests in Children with Henoch-schöenlein Purpura

Aim: This study aimed to investigate the in-vitro platelet aggregation and secretion tests in children patients with Henoch-schöenlein Purpura that recently referred to as Ig A vasculitis Methods: This is a cross-section study that included 55 patients with Henoch-schöenlein Purpura and 31 healthy children as a control group. Children who have a history of drug use, chronic diseases, and bleeding diseases were excluded from the study. Complete blood count, thrombocyte aggregation, and secretion tests were studied in both groups. These tests were re-evaluated in remission of the disease. Results: It was found that epinephrine-stimulated platelet aggregation and collagen, epinephrine, ristocetin, arachidonic acid, standard dose thrombin, and high dose thrombin-stimulated platelet secretion results were lower in the patients with Henoch-schöenlein Purpura compared to the healthy control group in the acute period (respectively P=0.014, 0,003; 0,003; 0,027; 0,034; 0,010; 0,049). When the values of patients with Henoch-schöenlein Purpura in the acute period and the remission of the disease were compared, collagen-stimulated platelet aggregation and epinephrine-stimulated platelet secretion values were found to be lower in patients with patients in the acute period (P= 0.016; 0.039) Conclusion: Impairment in vitro platelet aggregation and secretion tests in the patients with Henoch-schöenlein Purpura suggest that the tendency to bleeding in these patients may be due to platelet impairment function. Key Words: Henoch-schöenlein Purpura, platelet aggregation tests, platelet secretion tests, children, Ig A vasculitis. What’s already known about this topic? There is a tendency to bleeding in Henoch Shcöenlein Purpura patients, such as gastrointestinal bleeding, nonthrombocytopenic system purpura. What does this article add? It was found that impairment in-vitro platelet aggregation and secretion tests in Henoch Shcöenlein Purpura patients.

Functional Features of Platelet Secretion in Piglets During Early Ontogenesis

Physiologically, the secretion is considered to be a very important element of the platelet hemostasis system. Its functional state seriously determines the activity of primary hemostasis and microcirculation parameters in the capillaries, and, consequently, the course of metabolic processes throughout the body. It is known that the success of the entire hemostasis, as well as the activity of the processes in the development of the vessels of the body of productive animals, depends on the level of secretory activity of platelets. Of particular physiological significance are its features during early ontogenesis, when the body grows, matures and reaches its optimum productive qualities. For this reason, additional study of the secretory potential of platelets during the first year of life is of great importance for the physiology of the blood of piglets. These studies can help in finding approaches to the continuation of further intensification of pig production. In the work carried out, it was found that the ability of platelets to secrete increases in piglets during early ontogenesis. The main mechanism of this process should be considered as an increase in platelets of basal amount and self-assembly of actin and myosin. These changes are amplified at the physiological level in piglets due to the increase in the content of adenosine phosphate in platelet granules and the severity of its secretion under the influence of aggregation inducers. The increased activity of platelet secretion in piglets during the first year of life contributes to maintaining their microcirculation processes in the tissues at the level of their body's needs for nutrients and oxygen.

Alterations in platelet secretion differentially affect thrombosis and hemostasis

Key Points VAMP isoforms regulate the kinetics and extent of platelet granule exocytosis. Manipulating platelet sensitive factor attachment protein receptors alters granule secretion, which affects the hemostatic balance.