The normalised Sentinel-1 Global Backscatter Model, mapping Earth’s land surface with C-band microwaves

We present a new perspective on Earth’s land surface, providing a normalised microwave backscatter map from spaceborne Synthetic Aperture Radar (SAR) observations. The Sentinel-1 Global Backscatter Model (S1GBM) describes Earth for the period 2016–17 by the mean C-band radar cross section in VV- and VH-polarisation at a 10 m sampling. We processed 0.5 million Sentinel-1 scenes totalling 1.1 PB and performed semi-automatic quality curation and backscatter harmonisation related to orbit geometry effects. The overall mosaic quality excels (the few) existing datasets, with minimised imprinting from orbit discontinuities and successful angle normalisation in large parts of the world. Regions covered by only one or two Sentinel-1 orbits remain challenging, owing to insufficient angular variation and not yet perfect sub-swath thermal noise correction. Supporting the design and verification of upcoming radar sensors, the obtained S1GBM data potentially also serve land cover classification and determination of vegetation and soil states. Here, we demonstrate, as an example of its potential use, the mapping of permanent water bodies and evaluate against the Global Surface Water benchmark.

Investigation and Mitigation of Noise Contributions in a Compact Heterodyne Interferometer

We present a noise estimation and subtraction algorithm capable of increasing the sensitivity of heterodyne laser interferometers by one order of magnitude. The heterodyne interferometer is specially designed for dynamic measurements of a test mass in the application of sub-Hz inertial sensing. A noise floor of 3.31×10−11m/Hz at 100 mHz is achieved after applying our noise subtraction algorithm to a benchtop prototype interferometer that showed a noise level of 2.76×10−10m/Hz at 100 mHz when tested in vacuum at levels of 3×10−5 Torr. Based on the previous results, we investigated noise estimation and subtraction techniques of non-linear optical pathlength noise, laser frequency noise, and temperature fluctuations in heterodyne laser interferometers. For each noise source, we identified its contribution and removed it from the measurement by linear fitting or a spectral analysis algorithm. The noise correction algorithm we present in this article can be generally applied to heterodyne laser interferometers.

Correcting Artifacts in Ratiometric Biosensor Imaging; an Improved Approach for Dividing Noisy Signals

The accuracy of biosensor ratio imaging is limited by signal/noise. Signals can be weak when biosensor concentrations must be limited to avoid cell perturbation. This can be especially problematic in imaging of low volume regions, e.g., along the cell edge. The cell edge is an important imaging target in studies of cell motility. We show how the division of fluorescence intensities with low signal-to-noise at the cell edge creates specific artifacts due to background subtraction and division by small numbers, and that simply improving the accuracy of background subtraction cannot address these issues. We propose a new approach where, rather than simply subtracting background from the numerator and denominator, we subtract a noise correction factor (NCF) from the numerator only. This NCF can be derived from the analysis of noise distribution in the background near the cell edge or from ratio measurements in the cell regions where signal-to-noise is high. We test the performance of the method first by examining two noninteracting fluorophores distributed evenly in cells. This generated a uniform ratio that could provide a ground truth. We then analyzed actual protein activities reported by a single chain biosensor for the guanine exchange factor (GEF) Asef, and a dual chain biosensor for the GTPase Cdc42. The reduction of edge artifacts revealed persistent Asef activity in a narrow band (∼640 nm wide) immediately adjacent to the cell edge. For Cdc42, the NCF method revealed an artifact that would have been obscured by traditional background subtraction approaches.