The pathogenic biomarker alcohol dehydrogenase protein is involved in Bacillus cereus virulence and survival against host innate defence

Bacillus cereus is a spore forming bacteria recognized among the leading agents responsible for foodborne outbreaks in Europe. B. cereus is also gaining notoriety as an opportunistic human pathogen inducing local and systemic infections. The real incidence of such infection is likely underestimated and information on genetic and phenotypic characteristics of the incriminated strains is generally scarce. We have recently analyzed a large strain collection of varying pathogenic potential. Screening for biomarkers to differentiate among clinical and non-clinical strains, a gene encoding an alcohol dehydrogenase-like protein was identified among the leading candidates. This family of proteins has been demonstrated to be involved in the virulence of several bacterial species. The relevant gene was knocked out to elucidate its function with regards to resistance to host innate immune response, both in vitro and in vivo. Our results demonstrate that the adhB gene plays a significant role in resistance to nitric oxide and oxidative stress in vitro, as well as its pathogenic ability with regards to in vivo toxicity. These properties may explain the pathogenic potential of strains carrying this newly identified virulence factor.

Undrained Elastoplastic Solution for Cylindrical Cavity Expansion in Structured Cam Clay Soil Considering the Destructuration Effects

Soil structure has significant influences on the mechanical behaviors of natural soils, although it is rarely considered in previous cavity expansion analyses. This paper presents an undrained elastoplastic solution for cylindrical cavity expansion in structured soils, considering the destructuration effects. Firstly, a structural ratio was defined to denote the degree of the initial structure, and the Structured Cam Clay (SCC) model was employed to describe the subsequent stress-induced destructuration, including the structure degradation and crushing. Secondly, combined with the large strain theory, the considered problem was formulated as a system of first-order differential equations, which can be solved in a simplified procedure with the introduced auxiliary variable. Finally, the significance and efficiency of the present solution was demonstrated by comparing with the previous solutions, and parametric studies were also conducted to investigate the effects of soil structure and destructuration on the cavity expansion process. The results show that the soil structure has pronounced effects on the mechanical behavior of structured soils around the cavity. For structured soils, a cavity pressure that is larger than the corresponding reconstituted soils when the cavity expands to the same radius is required, and the effective stresses first increase to a peak value before decreasing rapidly with soil structure degradation and crushing. The same final critical state is reached for soils with different degrees of the initial structure, which indicates that the soil structure is completely destroyed during the cavity expansion. With the increase of the destructuring index, the soil structure was destroyed more rapidly, and the stress release during the plastic deformation became more significant. Moreover, the present solution was applied in the jacking of a casing during the sand compact pile installation and in situ self-boring pressuremeter (SBPM) tests, which indicates that the present solution provides an effective theoretical tool for predicting the behavior of natural structured soils around the cavity.

Measurement of elastic properties of materials employing 3-D DIC in a Cornu’s experiment

Measurement of elastic properties, especially the Poisson's ratio, utilizing non-contact techniques in a tensile experiment is very challenging. This is primarily due to the poor spatial resolution and the large strain noise inherent to these techniques. The difficulty level increases many folds when Poisson's ratio of less elongating, stiffer, and/or brittle materials, like ceramics and ablatives, is measured. This paper reports a newer approach that employs 3-D digital image correlation (3-D DIC) in a Cornu's experiment to enable accurate measurement of elastic properties in a single test. The deflection field obtained from 3-D DIC in the form of anticlastic surfaces during Cornu's experiment is utilized for determining Poisson's ratio. In the same experiment, the elastic modulus is estimated using the center point deflection method. The proposed methods are validated with standard materials and extended to newly developed materials. Cornu's method with 3-D DIC can provide the elastic properties with ease and has many advantages over other conventional techniques.

Present-day stress field pattern in the Vrancea seismic zone (Romania) deduced from earthquake focal mechanism inversion

Vrancea seismogenic zone in the South-Eastern Carpathians is characterized by localized intermediate-depth seismicity. Due to its complex geodynamics and large strain release, Vrancea represents a key element in the Carpatho-Pannonian system. Data from a recently compiled catalogue of fault plane solutions (REFMC) are inverted to evaluate stress regime in Vrancea on depth. A single predominant downdip extensive regime is obtained in all considered clusters, including the crustal layers located above the Vrancea slab. The prevalent stress regime confirms previous investigations and requires some mantle-crust coupling. The S3 principal stress is close to vertical, while S1 and S2 are horizontal, oriented perpendicularly and respectively tangentially to the Carpathians Arc bend. This configuration is present at any depth level. According to seismicity patterns, there are two main active segments in the Vrancea intermediate-depth domain, at 55 – 105 km and 105 – 180 km, both able to generate major events. The configuration of the tectonic stresses as resulted from inversion is similar in both segments. Also, high fault instability (I > 0.95) is characterizing the segments. The only notable difference is given by the friction and stress ratio parameters which drop down in the bottom segment from μ = 0.95 to μ = 0.55 and from R = 0.51 to R = 0.29. This variation is attributed to possible weakening processes activated below 100 km depth and can explain the intensification of seismicity production as earthquake rate and average energy release in the lower segment versus the upper segment.

Deformation of Poly-l-lactid acid (PLLA) under Uniaxial Tension and Plane-Strain Compression

The ability of PLLA, either amorphous or semicrystalline, to plastic deformation to large strain was investigated in a wide temperature range (Td = 70–140 °C). Active deformation mechanisms have been identified and compared for two different deformation modes—uniaxial drawing and plane-strain compression. The initially amorphous PLLA was capable of significant deformation in both tension and plane-strain compression. In contrast, the samples of crystallized PLLA were found brittle in tensile, whereas they proved to be ductile and capable of high-strain deformation when deformed in plane-strain compression. The main deformation mechanism identified in amorphous PLLA was the orientation of chains due to plastic flow, followed by strain-induced crystallization occurring at the true strain above e = 0.5. The oriented chains in amorphous phase were then transformed into oriented mesophase and/or oriented crystals. An upper temperature limit for mesophase formation was found below Td = 90 °C. The amount of mesophase formed in this process did not exceed 5 wt.%. An additional mesophase fraction was generated at high strains from crystals damaged by severe deformation. After the formation of the crystalline phase, further deformation followed the mechanisms characteristic for the semicrystalline polymer. Interlamellar slip supported by crystallographic chain slip has been identified as the major deformation mechanism in semicrystalline PLLA. It was found that the contribution of crystallographic slip increased notably with the increase in the deformation temperature. The most probable active crystallographic slip systems were (010)[001], (100)[001] or (110)[001] slip systems operating along the chain direction. At high temperatures (Td = 115–140 °C), the α→β crystal transformation was additionally observed, leading to the formation of a small fraction of β crystals.

Shallow Compaction Modeling and Upscaling: A One-Dimensional Analytical Solution and Upscaling

Natural depositional processes frequently give rise to the heterogeneous multilayer system, which is often overlooked but essential for the simulation of a geological process. The sediments undergo the large-strain process in shallow depth and the small-strain process in deep depth. With the transform matrix and Laplace transformation, a new method of solving multilayer small-strain (Terzaghi) and large-strain (Gibson) consolidations is proposed. The results from this work match the numerical results and other analytical solutions well. According to the method of transform matrix which can consider the integral properties of multilayer consolidation, a relevant upscaling method is developed. This method is more effective than the normally used weighted average method. Correspondingly, the upscaling results indicate that the upscaled properties of a multilayer system vary in the consolidation process.