Screening of grapevine red blotch virus in two European ampelographic collections

Grapevine red blotch virus (GRBV) is a recently identified virus that infects grapevine and has a severe impact on the grape industry in North America. Since the first description of the virus 8 years ago, clear progress has been made regarding our understanding of the GRBV pathosystem. However, questions remain regarding the origin of this pathogen and its spread outside North America, especially in Europe. In this study, we present the results of a large-scale GRBV survey in two European repositories; we targeted Vitis spp. accessions with diverse geographical origins. Of 816 accessions from different origins (50 different countries around the world), six accessions were infected by GRBV, all of which originated from the United States. We investigated the DNA virome of 155 grapevine accessions from the Swiss grapevine collection using high-throughput sequencing. We observed that virome of the Swiss grapevine collection was composed of several RNA viruses. In contrast, we did not detect any DNA viruses in the 155 Swiss grapevine accessions. This finding suggests that the abundance of DNA viruses infecting grapevines in Switzerland is either very low or non-existent. Our results and the findings of studies published since 2008 show that GRBV most likely originated in North America and subsequently spread to other viticultural areas in the world via unintentional movement of infected cuttings. According to our data, the most plausible scenario for the origin of GRBV is that the virus evolved from non-Vitis vinifera hosts and underwent a host jump to Vitis vinifera after its introduction to North America in the 1600s.

Mining machines elements packing and securing on platform container

The scientific purpose of this paper was to analyse the problem related to intermodal transportation of mining components packed in containers or other cargo transport units coupled with the problem of its proper securing. In this article, the issue of exposing the load to the effects of inertia forces which might cause unintentional movement is presented. The methods of securing the heavy load in cargo transport units are reviewed in the context of cargo immobilisation possibilities while reducing the load sensitivity to mechanical forces. The research part of this article presents the methods of packing and securing an atypical load, which is a part of a mining machine weighing 18t. This paper presents the results of calculations of inertia forces acting on the transported cargo, packed on a container platform. Based on the results, the cross fixing method was selected to secure the cargo and further decisions were made on the type and quantity of conveyor lashings necessary for the safe and correct carriage of the atypical load.

The Effect of ShotBlocker on Pain and Patient Satisfaction for Spinal Anesthesia: A Randomized Trial

BACKGROUND: During spinal anesthesia, patients may experience pain and discomfort associated with dermal puncture. It may also cause involuntary movement, which often disturbs the patient’s posture thus affecting the success of spinal anesthesia. Different methods have been studied to cope with needle-related pain. “ShotBlocker” is a flexible, plastic, U-shaped device, which has several blunt points. It is suggested that blunt points provide a nonnoxious physical stimulation and inhibit the transmission of injection pain. OBJECTIVES: The purpose of this study was to examine the effectiveness of the ShotBlocker for dermal puncture pain during spinal anesthesia. STUDY DESIGN: Prospective randomized trial. SETTING: University hospital, operating room. METHODS: Ninety-four patients aged 18 to 65 years with physical status American Society of Anesthesiologists (ASA) I or II scheduled for elective surgery under spinal anesthesia were randomly assigned to either a ShotBlocker (n = 47) or control group (n = 47). In the ShotBlocker group, lumbar punctures were performed with the application of ShotBlocker. Needle-associated pain score and patient satisfaction were assessed by the patient, immediately after completion of the spinal anesthesia using a 10-cm Visual Analog Scale (VAS) and a 5-point Likert scale for satisfaction. Patients were also closely observed for symptoms of discomfort such as spontaneous vocalization and involuntary movement. RESULTS: A total of 88 patients completed the study. There was no significant difference in VAS scores, patient satisfaction, or procedure time between groups (P > 0.05). Spontaneous vocalization did not occur in both groups. However, incidence of unintentional movement during dermal puncture was higher in the control group (6.8% vs. 31.8%; P < 0.05). LIMITATIONS: Study was conducted in a single-center with a relatively small population of patients. Only the attending anesthetist collecting data was blinded to the procedure. Patients older than age 65 years were also excluded from the study, thus our results cannot be generalized. CONCLUSIONS: ShotBlocker did not show any advantage on VAS scores but decreased the incidence of unintentional movement during dermal puncture. KEY WORDS: Spinal anesthesia, pain, ShotBlocker, needle, pin-prick pain, dermal puncture, neuraxial block, regional, needle phobia, lumbar puncture

Isolation of Streptomycin-Resistant Isolates of Erwinia amylovora in New York

Streptomycin is currently the only antibiotic registered for the control of fire blight, a devastating disease of apple (Malus), pear (Pyrus), and other rosaceous plants caused by the bacterium Erwinia amylovora. Resistance of E. amylovora to streptomycin was first identified in California pear orchards in 1971 and is currently endemic in many parts of the United States. The Northeast remains the only major U.S. apple-growing region without streptomycin-resistant isolates of E. amylovora. In 2002, during a routine survey for streptomycin resistance, isolates from two neighboring orchards in Wayne County, NY were found to be highly resistant to streptomycin at a concentration of 100 μg/ml. This constitutes the first authenticated report of streptomycin resistance in New York State. Infected trees were shipped at the same time from a single nursery in Michigan. Resistance was caused by the acquisition of the strA-strB gene pair, inserted into the ubiquitous nontransmissible E. amylovora plasmid pEA29. Previously, streptomycin-resistant E. amylovora populations from Michigan were described with a similar mechanism of resistance, although the strA-strB genes are not unique to Michigan. These findings illustrate how unintentional movement of nursery material could undermine efforts to prevent the spread of antibiotic-resistant E. amylovora.

Human-assisted Movements of Raccoons, Procyon lotor, and Opossums, Didelphis virginiana, between the United States and Canada

Movements of Raccoons (Procyon lotor) (mean = 479 km) and Opossums (Didelphis virginiana) (mean = 688 km) by means of transport trailers and a train from the USA and Quebec into Ontario were significantly greater than Raccoon movements (mean = 15 km) from Ontario to New York State determined by mark-recapture. Human-assisted movements of wildlife could have significant impacts with respect to cross-border movements of diseases such as rabies. Proactive communication programs, especially at International border crossing areas, should be encouraged to decrease the occurrence of the unintentional movement of wildlife and associated diseases.

Ernst Cassirer, Theoretical Biology, and the Clever Hans Phenomenon

The ArgumentBiology, understood in turn-of-the-century Germany to include psychology, held a central but enigmatic place in the philosopher Ernst Cassirer's work. From his earliest studies with Hermann Cohen through his long engagement with the theoretical biology of Jakob von Uexküll and Adolf Meyer-Abich, Cassirer consistently used the history and practice of biology to examine and delineate a set of characteristic tensions between the natural and cultural sciences. This paper examines Cassirer's treatment of this theme by addressing two contrasting interpretations he gave — in his Philosophy of Symbolic Forms (1929) and in his Essay on Man (1944) — to the benchmark case from empirical psychology of the “talking” horse “Clever Hans.” The original case involved the horse's ability to signal answers to remarkably complex questions by stamping its hooves, an ability that ultimately appeared to rest on a capacity to detect extremely minute unintentional movement cues in its auditors as it reached the appropriate answer. Due to both Cassirer's shifting description of the case within his philosophy and the case's inherent polyvalence, Cassirer's remarks provide a useful window onto the social, epistemological, and stylistic meaning of his “unified” philosophy of human culture and science.