Evolution of Shock-Induced Pressure in Laser Bioprinting

Laser bioprinting with gel microdroplets that contain living cells is a promising method for use in microbiology, biotechnology, and medicine. Laser engineering of microbial systems (LEMS) technology by laser-induced forward transfer (LIFT) is highly effective in isolating difficult-to-cultivate and uncultured microorganisms, which are essential for modern bioscience. In LEMS the transfer of a microdroplet of a gel substrate containing living cell occurs due to the rapid heating under the tight focusing of a nanosecond infrared laser pulse onto thin metal film with the substrate layer. During laser transfer, living organisms are affected by temperature and pressure jumps, high dynamic loads, and several others. The study of these factors’ role is important both for improving laser printing technology itself and from a purely theoretical point of view in relation to understanding the mechanisms of LEMS action. This article presents the results of an experimental study of bubbles, gel jets, and shock waves arising in liquid media during nanosecond laser heating of a Ti film obtained using time-resolving shadow microscopy. Estimates of the pressure jumps experienced by microorganisms in the process of laser transfer are performed: in the operating range of laser energies for bioprinting LEMS technology, pressure jumps near the absorbing film of the donor plate is about 30 MPa. The efficiency of laser pulse energy conversion to mechanical post-effects is about 10%. The estimates obtained are of great importance for microbiology, biotechnology, and medicine, particularly for improving the technologies related to laser bioprinting and the laser engineering of microbial systems.

Pulses on Demand in Fibre and Hybrid Lasers

This paper presents an investigation of pulse-on-demand operation in fibre and hybrid lasers. Two methods for efficient gain control that enable the generation of laser pulses at arbitrary times with controlled pulse parameters are presented. The method of direct modulation of the pump power in the high-power laser oscillator is shown to generate pulses with a duration in the nanosecond range, with repetition rates varying during operation from a single shot to over 1 MHz. An advanced method using a combination of marker and idler seeding a fibre amplifier chain is investigated. Such a system can easily achieve repetition rates of several tens of MHz. The lasers’ performances were successfully tested in a real environment on an industrial platform for laser transfer printing. Similar concepts were used for a laser source with ultrashort laser pulses (femtosecond range) on demand by using a mode-locked seed as a source and a solid-state amplifier to achieve high pulse energy and peak power.

Esterification Process Induced by UV Irradiation of Shellac thin Films Deposited by Matrix Assisted Pulsed Laser Evaporation

In this work we report on the deposition of UV resistant shellac thin films by matrix assisted pulsed laser evaporation (MAPLE). The films obtained starting from a solution of 1-2 wt% shellac in methanol were very smooth and their chemical structure was preserved after the laser transfer. Upon short exposure time (u to 60 min), UV irradiation did not induce significant modifications in the films composition and surface appearance, while upon increasing the irradiation time (240 min) an esterification process of shellac can be observed.

Shear-Assisted Laser Transfer of Metal Nanoparticle Ink to an Elastomer Substrate

Selective laser sintering of metal nanoparticle ink is an attractive technology for the creation of metal layers at the microscale without any vacuum deposition process, yet its application to elastomer substrates has remained a highly challenging task. To address this issue, we introduced the shear-assisted laser transfer of metal nanoparticle ink by utilizing the difference in thermal expansion coefficients between the elastomer and the target metal electrode. The laser was focused and scanned across the absorbing metal nanoparticle ink layer that was in conformal contact with the elastomer with a high thermal expansion coefficient. The resultant shear stress at the interface assists the selective transfer of the sintered metal nanoparticle layer. We expect that the proposed method can be a competent fabrication route for a transparent conductor on elastomer substrates.