Abstract
An urgent task is the development of devices that provide a precision temperature change. The devices used in the real-time polymerase chain reaction method require not only repeated cyclic temperature changes with high accuracy, but also the temperature gradient minimization over the working fluid. In this article, the modeling of the working processes taking place in the thermal block of the device with the previously proposed thermohydraulic system, which ensures the temperature change together with the Peltier elements, is carried out. A mathematical model is formulated and a differential equation, boundary and initial conditions are substantiated. Numerical methods for solving the mathematical model in the Elcut software were applied. An experimental setup was developed and a study of heat exchange processes in the thermal block of a nucleic acid analyzer with a thermohydraulic system was carried out, confirming the adequacy of the developed mathematical model.