Hormonal Regulation of Early Fruit Development in European Pear (Pyrus communis cv. ‘Conference’)

European pear requires inter-cultivar cross-pollination by insects to develop fertilized fruits. However, some European pear cultivars such as ‘Conference’ naturally produce parthenocarpic seedless fruits. To better understand the hormonal regulation of fruit set and early fruit development in this European pear cultivar, the phytohormone and polyamine profiles in ‘Conference’ flowers and fruits resulting from both fertilization and parthenocarpic processes were analyzed. The expression of genes involved in phytohormone metabolism and signaling were also investigated. Phytohormone profiles differed more at flower stage 3 days after treatment than in 15 day- and 30-day-old fruits in response to fertilization and parthenocarpy. An increase in auxins, abscisic acid, ethylene precursor, and spermine, and a decrease in putrescine were recorded in the fertilized flowers as compared to the parthenocarpic flowers. Fertilization also upregulated genes involved in gibberellin synthesis and down-regulated genes involved in gibberellin catabolism although the total gibberellin content was not modified. Moreover, exogenous gibberellin (GA3, GA4/7) and cytokinin (6BA) applications did not increase parthenocarpic induction in ‘Conference’ as observed in other European and Asian pear cultivars. We hypothesize that the intrinsic parthenocarpy of ‘Conference’ could be related to a high gibberellin level in the flowers explaining why exogenous gibberellin application did not increase parthenocarpy as observed in other pear cultivars and species.

Effect of cotyledons and epicotyl upon the activity of endogenous gibberellins in roots of flax (Linum usitatissimum L.) seedlings

In 11-day-old flax seedlings, the level of endogenous gibberellins in roots decreased within 12 hours after the excision of cotyledons and the epicotyl; however, 24 to 48 hours after excision the gibberellin level increased again. The decrease in the gibberellin level within the first 12 hours after excision suggests a participation of the cotyledons and the epicotyl in the biosynthesis of endogenous gibberellins.

Gibberellin and the Growth of Peach and Apricot Fruits

Levels of gibberellin in purified extracts from developing peach fruits were compared with rates of cell division and cell expansion in the fruit tissues. No gibberellin was found in the ovary before full bloom. Immediately after full bloom gibberellin activity was found in the seed, and later in the mesocarp and endocarp as well. Gibberellin concentration was closely correlated with the rate of cell expansion in each tissue, but not with cell division. Until the final growth phase, when activity was found only in the mesocarp, the highest gibberellin level was always found in the seed.