scholarly journals The effect of biopreparations of nodules bacteria with carmoisine on the formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic systems

2021 ◽  
Vol 17 (2) ◽  
pp. 123-129
Author(s):  
Ekaterina Kukol ◽  
Nadiya Vorobey ◽  
Petro Pukhtaievych ◽  
Sergii Kots
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Negative Impact ◽  
Root Nodules ◽  
Soybean Seeds ◽  
Biological Products ◽  
Dye Analysis ◽  
Full Flowering ◽  
Negative Effect ◽  
Glycine Max L ◽  
Beginning Of Flowering

Purpose. Investigate the formation and functioning of symbiotic systems of soybeans with nodule bacteria by ino­culation of seeds with biological products based on fungicide-resistant strains of Bradyrhizobium japonicum PC07 and B78 with different rates of synthetic carmoisine colorant. Methods. Physiological, microbiological, gas chromatography, statistical. Results. It was found that as a result of inoculation of soybean [Glycine max (L.) Merr] variety ‘Almaz’ with microbial preparations based on B. japonicum PC07 and B78, with the addition of carmoisine (0.25 and 0.5 g per 200 g of the preparation), the amount and the weight of nodules formed on the roots during the growing season were at the level of the control plants or exceeded them. The greatest difference in indicators of quantity and weight of root nodules between plants of control and experimental variants is noted in a phase of full flowering at inoculation by both strains of rhizobia and addition to biological products of various norms of dye. Analysis of nitrogen-fixing activity (NFA) of the formed symbiotic systems showed the absence of a negative effect of the synthetic colorant on its level. When inoculated with soybean seeds B. japonicum PC07 in the phase of three true leaves, NFA was higher by 15.6–25.9% and in the budding-beginning of flowering stage by 7.4–29.5% compared with control plants with the addition of 0.25 and 0.5 g of carmoisine, respectively. Against the background of bacterization of soybean seeds by strain B78 before the phase of full flowering of plants the level of N2 assimilation by adding 0.25 g of carmoisine to the vermiculite preparation was at the level of the control plants. During the period of full flowering, this figure exceeded the indicators of control plants by 7.6 and 18.8% with the introduction of 0.25 and 0.5 g of the colorant. Conclusions. Carmoisine can be applied in the further study of the effectiveness of its use as a dye identifier for controlling the uniformity of marking of loose bacterial preparations on seeds by adding 0.25 and 0.5 g per 200 g of a biopreparation, since this did not show a negative impact on the formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic systems.

scholarly journals PHYSIOLOGICAL INTERACTION OF SOYBEAN PLANTS AND NEW TN-5 MUTANTS OF BRADYRHIZOBIUM JAPONICUM

2009 ◽  
Vol 8 ◽  
pp. 52-61
Author(s):  
V.K. Datsenko ◽  
V.M. Mel’nyk ◽  
S.Ya. Kots ◽  
S.V. Omel’chuk
Keyword(s):  
Host Plant ◽  
Bradyrhizobium Japonicum ◽  
Direct Relationship ◽  
Root Nodules ◽  
Soybean Seeds ◽  
Nitrogen Fixation Activity ◽  
Soybean Plants ◽  
Symbiotic Properties ◽  
Relationship Of ◽  
Highly Virulent

The influence of soybean seeds inoculation with Tn-5 mutants of Bradyrhizobium japonicum with polar symbiotic properties on symbiosis efficiency, photosynthetic intensity and activity of antioxidant enzymes in root nodules of host plant was studied. Most of the selected mutants were highly virulent, but as was shown there were no considerable correlation between studied parameters. The direct relationship of nitrogen fixation activity of roots nodules and photosynthesis intensity of host plant was established. The two types of dynamics of theses processes were determined with their maximum values in blooming and flowering stages, respectively.  

scholarly journals Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions

2020 ◽  
Vol 25 (50) ◽  
pp. 105-111
Author(s):  
Zlatica Miladinov ◽  
Ivana Maksimović ◽  
Svetlana Balešević-Tubić ◽  
Vojin Đukić ◽  
Zorica Nikolić ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Water Potential ◽  
Negative Impact ◽  
Cultivated Plants ◽  
Soybean Seeds ◽  
Sensitive Stage ◽  
Negative Effect ◽  
Improve Seed Germination ◽  
Successful Production

Drought is one of the most important factors limiting the successful production of cultivated plants. One of the most sensitive stage to the water deficit in plants is seed germination. There are various methods of pre-sowing treatments that aim to reduce the negative impact of drought stress and improve seed germination. One of them is priming seeds. The aim of this experiment was to examine the effect of priming seeds in solutions - KNO3 (1%), ascorbic acid - AsA (100 mgl-1) and potassium chloride KCl (1%) on the reduction of the negative effect of drought stress. The effect of drought stress was simulated using different concentrations of PEG 6000 (0 (control), -0.30, -0.51, -0.80 MPa). The results of the research showed that with the increase of water deficiency, the effect of priming seeds is greater. At the water potential of the solution of -0.30 MPa, germination energy and seed germination increased on average by 6.77% and 5.08%, while at the water potential of the solution of -0.80 MPa, the increase was 19.28% and 16.75%, respectively. Also, priming seeds significantly reduced the intensity of lipid peroxidation and the content of free proline. From all the above, it can be concluded that priming of seeds is a method that can serve to improve the germination of soybean seeds in conditions of drought stress.

scholarly journals Molecular identification of Bradyrhizobium japonicum strains isolated from root nodules of soybean (Glycine max L.)

2017 ◽  
pp. 49-56
Author(s):  
Jelena Marinkovic ◽  
Dragana Bjelic ◽  
Branislava Tintor ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Biochemical Properties ◽  
Universal Primers ◽  
Local Alignment ◽  
Molecular Characteristics ◽  
Bradyrhizobium Sp ◽  
Search Tool ◽  
Glycine Max L

The aim of this study was to isolate and identify Bradyrhizobium japonicum strains on the basis of molecular characteristics. From root nodules of different soybean cultivars were obtained 56 isolates, characterized according to morphological, cultural, and biochemical properties. Among these isolates, 33 isolates showing resemblance with Bradyrhizobium sp. were further subjected to molecular identification. Following DNA extraction, a partial 16S rDNA gene sequence from the isolates was amplified by PCR using universal primers fD1 (27F) and rP3 (1492R). Purification and sequencing of the amplified fragments were done in the bio?technology company Macrogen, Seoul, South Korea. Sequences were analyzed using the pro?gram FinchTV and BLAST (Basic Local Alignment Search Tool) and compared to sequences in GenBank and the Bradyrhizobium ID-database for identification. Comparison of the se?quences with the Bradyrhizobium ID-database showed that all tested isolates were identified as Bradyrhizobium japonicum. Each isolate was deposited in the NCBI GenBank database under a unique accession number. Identification of Bradyrhizobium species from root nodules of soybean is of great importance because the symbiosis between rhizobia and legumes are a cheaper and usually more effective agronomic practice for ensuring an adequate supply of nitrogen for legumes, while preserving and improving fertility and productivity of soils.

scholarly journals LEGUME LECTINS ACTIVATE SYMBIOTIC PROPERTIES OF SPECIFIC RHIZOBIA UNDER SYMBIOSIS FORMATION AND FUNCTIONING

2009 ◽  
Vol 9 ◽  
pp. 43-58
Author(s):  
O.V. Kyrychenko
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Nodule Bacteria ◽  
Plant Host ◽  
Pisum Sativum L ◽  
Glycine Max L ◽  
Growth Formation ◽  
Symbiotic Properties ◽  
Legume Lectins

The effect of pea and soybean lectins on the symbiotic properties of nodule bacteria Rhizobium leguminosarum bv. viciae 240б and Bradyrhizobium japonicum 634б during the formation and functioning of symbiosis with pea (Pisum sativum L.) and soybean (Glycine max L. Merr.) plants respectively was investigated. It was shown that incubation of homologous rhizobia with plant-host lectin had increased bacteria nodulation ability at seeds inoculation, nitrogen fixing activity of root nodules and symbiosis efficiency that had resulted in intensification of plants’ photosynthesis, positive changes of legumenous plants growth, formation of vegetative mass and harvest. The use of complex of complex compositions based on the legume lectins and homologous rhizobia for pre-sowing seeds treatment was shown to be perspective in order to increase seed productivity of legumenous plants.

scholarly journals Respostas de Componentes Produtivos de Soja a Inoculação de Biológicos em Campo

2021 ◽  
Vol 24 (5-esp.) ◽  
pp. 576-583
Author(s):  
Jorge González Aguilera ◽  
Alan Mario Zuffo ◽  
Rafael Felippe Ratke ◽  
Renato Jaqueto Goes ◽  
Natielly Pereira Da Silva ◽  
...  
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Soybean Seeds ◽  
Agronomic Performance ◽  
Biological Products ◽  
Tillage System ◽  
Physiological Quality ◽  
Relative Productivity

O emprego de produtos biológicos no sistema de plantio direto constitui-se uma prática sustentável e com menor potencial poluente que o uso de produtos químicos. Nesta pesquisa objetivou-se avaliar o desempenho agronômico de três cultivares de soja após a inoculação pós emergente de biológicos no solo. O delineamento experimental utilizado foi o de blocos casualizados em esquema em parcelas subdivididas 3×5, com três repetições. Os tratamentos foram a combinação de três cultivares (BRASMAX-FOCO-IPRO, BRASMAX-DESAFIO-IPRO e BRASMAX-BÔNUS-IPRO) e quatro biológicos [Azospirillum brasilense + Bradyrhizobium japonicum (1), BioAx (2), Trichoderma asperrellum (3), 1+2+3 (4)], mais um Controle (ausência de biológicos) (5). Foi avaliado a altura das plantas (AP), inserção do primeiro legume (IPL), número de legume por planta (PNL), número de grãos por legumes (NGL), massa de mil grãos (MMG) e produtividade (PROD). Os grãos colhidos, foram classificados por tamanho em peneiras de diâmetros de 7.5, 7.0, 6.5, 6.0, 5.5, 5.0 e 4.5 mm. A porcentagem de grãos retidos (RP) e a produtividade relativa de grãos retidos nas peneiras (PRGRP) foi calculada. Os resultados apontaram interações significativas (p<0,01) para as variáveis NGL, NGP, MMG, Ø6.5, Ø6.0, Ø5.5, Ø5.0, RP e PRGRP. O emprego de biológicos aplicados de modo post-emergente promove uma melhor qualidade fisiológica das sementes de soja se comparadas ao controle, ao considerar os diâmetros de Ø6.0 e Ø6.5.   Palavras-chave: Glycine max. Qualidade das Sementes. Azospirillum brasilense; Bradyrhizobium japonicum. BioAx; Trichoderma asperrellum.   Abstract The use of biological products in the no-tillage system is a sustainable practice with less polluting potential than the use of chemicals. This research aimed to evaluate the agronomic performance of three soybean cultivars after the post-emergence inoculation of biologicals in the soil. The experimental design used was randomized blocks in a  3 × 5 subdivided plots scheme, with three replications. The treatments were the combination of three cultivars (BRASMAX-FOCO-IPRO, BRASMAX-DESAFIO-IPRO and BRASMAX-BÔNUS-IPRO) and four biological [Azospirillum brasilense + Bradyrhizobium japonicum (1), BioAx (2), Trichoderma asperrellum (3) , 1 + 2 + 3 (4)], plus a Control (absence of biological products) (5). Plant height (PH), insertion of the first pods (IFP), number of pods per plant (NPP), number of grains per pods (NGP), mass of one thousand grains (MTG) and productivity (PROD) were evaluated. The harvested grains were classified by size in sieves with diameters of 7.5, 7.0, 6.5, 6.0, 5.5, 5.0 and 4.5 mm. The percentage of grains retained (GR) and the relative productivity of grains retained in the sieves (RPGRS) was calculated. The results showed significant interactions (p<0.01) for the variables NGP, NPP, MTG, Ø6.5, Ø6.0, Ø5.5, Ø5.0, GR and RPGRS. The use of biologically applied post-emergence promotes a better physiological quality of soybean seeds compared to the control, when considering the diameters of Ø6.0 and Ø6.5.   Keywords: Glycine max. quality of seeds. Azospirillum brasilense. Bradyrhizobium japonicum. BioAx; Trichoderma asperrellum.  

PD-L1 and survival in oral cavity squamous cell carcinoma

2020 ◽  
Vol 25 (4) ◽  
pp. 287-294
Author(s):  
S. I. Kutukova ◽  
N. P. Beliak ◽  
G. A. Raskin ◽  
M. S. Mukhina ◽  
Yu. V. Ivaskova ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Immune Cells ◽  
Negative Impact ◽  
Progression Free Survival ◽  
Negative Effect ◽  
Effect Of Pd

Relevance. Prognostic value of PD-L1 expression in oral cavity squamous cell carcinoma (OCSCC) and its effect on survival is still controversial. It should be to determine the prognostic role of PD-L1 expression on tumor and immune cells of OCSCC and assess their effect on overall survival (OS) and progression-free survival (PFS).Materials and methods. A prospective study included 145 patients, first diagnosed with OCSCC. PD-L1 expression on tumor and immune cells, infiltrating tumor and its microenvironment, was assessed in all tumor samples by IHC, CPS was calculated. Cut-off values were determined by ROC analysis for identification of PD-L1 expression effect on OS and PFS.Results. Most patients with oral mucosa squamous cell carcinoma showed positive expression of PD-L1 on tumor (77.2%) and immune cells (92.4%). The median PD-L1 expression on tumor cells was 13.5% [1.0-40.0], the median PD-L1 expression on immune cells was 5.0% [1.0-11.0], and the median CPS – 18.0 [3.0-7.8]. Univariate and multivariate analyses revealed a significant negative effect of PD-L1 expression on immune cells ≤ 7% on OS (HR 0.66; 95% CI 0.45-0.93; p = 0.0498); PD-L1 expression in tumor cells ≤ 15% (HR 0.65; 95% CI 0.43-0.98; p = 0.0416) and CPS ≤ 21 (HR 0.62; 95% CI 0.44-0.92; p = 0.0183) for PFS. PD-L1 expression in tumor cells ≤ 6% (HR 0.71; 95% CI 0.47-1.08; p = 0.1096) and CPS ≤ 7 (RR 0.67; 95% CI 0.44-1.01; p = 0.0575) had a confident tendency to negative impact on OS.Conclusion. Positive PD-L1 expression in tumor and immune cells as well as CPS are effective additional factors in the prognosis of the disease course, OS and PFS in patients with OCSCC.

scholarly journals Relationships Among the Feeding Behaviors of a Mirid Bug on Cotton Leaves of Different Ages and Plant Biochemical Substances

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Haiyan Song ◽  
Zhaoke Dong ◽  
Lili Li ◽  
Zengbin Lu ◽  
Chao Li ◽  
...  
Keyword(s):  
Negative Impact ◽  
Leaf Age ◽  
Leaf Traits ◽  
Cotton Leaf ◽  
Young Plant ◽  
Feeding Behaviors ◽  
Probing Behavior ◽  
Negative Effect ◽  
Mirid Bug ◽  
The Relationship

Abstract Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) tends to feed on young plant tissues. To explore the relationship between stylet probing behaviors of adult A. lucorum and conditions of cotton leaves, we conducted an experiment using electropenetrography (EPG). Behaviors were recorded on four cotton varieties, in relation to thickness and biochemical traits of differently-aged leaves. Cotton leaf age had a significant effect on the probing behavior of A. lucorum but cotton variety did not. One-day-old leaves of A. lucorum received the highest mean number of stylet probes (penetrations) per insect, and longest mean durations per insect of combined stylet probing or its components, cell rupture and ingestion behaviors. All of the leaf traits (thickness and biochemical substances) were similar among these four cotton varieties. Leaf thickness had a significantly negative effect on the same four variables above. Gossypol and tannin also had a negative impact on combined probing duration. Redundancy analysis showed that the four EPG variables were closely related to nutrient substances (amino acids, sugar, and water) while they had the opposite relationship with plant defense substances (gossypol and tannin). On cotton in the seedling stages, A. lucorum fed more readily on the youngest, thinnest leaves in our no-choice EPG experiments. Nutrients and chemical resistance substances determined the probing duration of A. lucorum. Our findings can contribute to better understanding of patterns of feeding and host consumption by A. lucorum, ultimately improving cotton resistance to A. lucorum.

scholarly journals Effect of Nitrogen Fertilisation and Inoculation with Bradyrhizobium japonicum on the Fatty Acid Profile of Soybean (Glycine max (L.) Merrill) Seeds

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 941
Author(s):  
Ewa Szpunar-Krok ◽  
Anna Wondołowska-Grabowska ◽  
Dorota Bobrecka-Jamro ◽  
Marta Jańczak-Pieniążek ◽  
Andrzej Kotecki ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Fatty Acid Content ◽  
Soybean Seeds ◽  
Oilseed Crop ◽  
Soybean Cultivars

Soybean is a valuable protein and oilseed crop ranked among the most significant of the major crops. Field experiments were carried out in 2016–2019 in South-East Poland. The influence of soybean cultivars (Aldana, Annushka), nitrogen fertilizer (0, 30, 60 kg∙ha−1 N) and inoculation with B. japonicum (control, HiStick® Soy, Nitragina) on the content of fatty acids (FA) in soybean seeds was investigated in a three-factorial experiment. This study confirms the genetic determinants of fatty acid composition in soybean seeds and their differential accumulation levels for C16:0, C16:1, C18:1n9, C18:2, C18:3, and C20:0 as well saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids. Increasing the rate from 30 to 60 kg ha−1 N did not produce the expected changes, suggesting the use of only a “starter” rate of 30 kg ha−1 N. Inoculation of soybean seeds with a strain of Bradyrhizobium japonicum (HiStick® Soy, BASF, Littlehampton, UK and Nitragina, Institute of Soil Science and Plant Cultivation–State Research Institute, Puławy, Poland) is recommended as it will cause a decrease in SFA and C16:0 acid levels. This is considered nutritionally beneficial as its contribution to total fatty acids determines the hypercholesterolemic index, and it is the third most accumulated fatty acid in soybean seeds. The interaction of cultivars and inoculation formulation on fatty acid content of soybean seeds was demonstrated. An increase in the value of C16:0 content resulted in a decrease in the accumulation of C18:1, C18:2, and C18:3 acids. The content of each decreased by almost one unit for every 1% increase in C16:0 content. The dominant effect of weather conditions on the FA profile and C18:2n6/C18:3n3 ratio was demonstrated. This suggests a need for further evaluation of the genetic progress of soybean cultivars with respect to fatty acid composition and content under varying habitat conditions.

Metabolic Adjustment of Glycine max (L.) Merril in the Presence of Nitrate and Bradyrhizobium japonicum

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1518
Author(s):  
Alberto Mongolo Júnior ◽  
Felipe Girotto Campos ◽  
Gustavo Ribeiro Barzotto ◽  
Jonas Akenaton Venturineli Pagassini ◽  
Maria Aparecida Ribeiro Vieira ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Dry Mass ◽  
Antioxidant Systems ◽  
Oxygen Species ◽  
Membrane Stabilization ◽  
Total Sugars ◽  
Vegetative Phase ◽  
Glycine Max L ◽  
Metabolic Adjustment ◽  
Plant Dry Mass

Reactive oxygen species are generated during the processes of photosynthesis and nitrate reduction, which can compromise the integrity of biomolecules and membranes. During the vegetative phase of Fabaceae species, around half of translocated carbohydrate is used for nodule growth, while the other half returns to the aerial part with nitrogen incorporated. These sugars may be yet involved with membrane stabilization, signaling, and activation of important genetic pathways for plant development. Thus, the aim was to study the adjustments of the photosynthetic and antioxidant systems and the accumulation of carbohydrates and biomass in Glycine–Bradyrhizobium cultivated with nitrate (NO3−). Four treatments were evaluated in completely randomized blocks. Glycine–Bradyrhizobium was grown with 1.7 mM of NO3− (GB: 1.7 mM NO3−) and without NO3− (GB: 0 mM NO3−), and Glycine was grown with 1.7 mM of NO3− (G: 1.7 mM NO3−) and without NO3− (G: 0 mM NO3−). Glycine–Bradyrhizobium symbiosis contributes to photosynthetic metabolism and total sugars, reduces the action of antioxidant enzymes, and minimizes the use of nitrate in soybean cultivation.; Glycine–Bradyrhizobium with nitrate provided greater plant dry mass in the vegetative phase, along with increased enzymatic activity and reduced nodule mass.

scholarly journals Analysis of User Needs on Downloading Behavior of English Vocabulary APPs Based on Data Mining for Online Comments

Mathematics ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1341
Author(s):  
Tinggui Chen ◽  
Lijuan Peng ◽  
Jianjun Yang ◽  
Guodong Cong
Keyword(s):  
Negative Impact ◽  
Positive Impact ◽  
User Needs ◽  
User Requirements ◽  
Functional Requirements ◽  
User Comments ◽  
Online Comments ◽  
Significant Negative Impact ◽  
Negative Effect ◽  
New Type

With highly developed social media, English learning Applications have become a new type of mobile learning resources, and online comments posted by users after using them have not only become an important source of intellectual competition for enterprises, but can also help understand customers’ requirements, thereby improving product functionalities and service quality, and solve the pain points of product iteration and innovation. Based on this, this paper crawled the online user comments of three typical APPs (BaiCiZhan, MoMoBeiDanCi and BuBeiDanCi), through emotion analysis and hotspot mining technology, to obtain user requirements and then the K-means clustering method was used to analyze user requirements. Finally, quantile regression is used to find out which user needs have an impact on the downloads of English vocabulary APPs. The results show that: (1) Positive comments have a more significant impact on users’ downloads behavior than negative online comments. (2) English vocabulary APPs with higher downloads, both the 5-star user ratings and the increase of emotional requirement have a negative effect on the increase in APP downloads, while the enterprise’s service requirement improvement has a positive effect on the increase of APP downloads. (3) Regarding English vocabulary APPs with average or high downloads, improving the adaptability and Appearance requirements have significant negative impact on downloads. (4) The functional requirements to improve products will have a significant positive impact on the increase in downloads of English vocabulary APPs.

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