Ontogenetic niche shifts in a locally endangered tree species (Olea europaea subsp. cuspidata) in a disturbed forest in Northern Ethiopia: Implications for conservation

Understanding the responses of different ontogenetic stages to environmental and human disturbance factors is essential for developing efficient conservation strategies for endangered plant species. We examined how three ontogenetic stages of a locally endangered tree species, Olea europaea subsp. cuspidata, responded to environmental factors and human disturbance in Hugumburda dry Afromontane forest in Ethiopia. We counted individual seedlings, saplings and adults of O. europaea in 70 20 × 20 m quadrats over ca. 2.8 ha, and measured biotic (woody species richness, canopy cover, aboveground tree biomass, herbaceous cover), abiotic (soil and topographic variables), and human disturbance factors (logging and tracks). To detect ontogenetic niche shifts, we compared observed vs. simulated locations of trees in the three life stages and how they related to the environmental and human disturbance factors. We found that the population structure of O. europaea showed generally low recruitment, with few seedlings per hectare compared with the abundance of saplings and adults. The probability of finding O. europaea individuals was influenced by biotic (woody species richness) and abiotic (soil depth, slope) environmental conditions and human disturbance (logging intensity), but the direction, strength and shape of the relationships differed between seedling, sapling and adult life stages, indicating ontogenetic niche shifts. All life stages showed a positive relationship with elevation. The observed environmental niches of the different lifestages of O. europaea, and their association with human disturbance levels, should be considered when conservation strategies are developed for this species. Human disturbance in terms of logging decreases the abundance of saplings, but may facilitate emerging seedlings through creation of gaps with improved light conditions. Recruitment is, however, very low in the study area, and seedlings should be protected from browsing to enhance survival. Woody species richness in general should be conserved to optimize conditions also for O. europaea saplings.

Juvenile ecology drives adult morphology in two insect orders

Most animals undergo ecological niche shifts between distinct life phases, but such shifts can result in adaptive conflicts of phenotypic traits. Metamorphosis can reduce these conflicts by breaking up trait correlations, allowing each life phase to independently adapt to its ecological niche. This process is called adaptive decoupling. It is, however, yet unknown to what extent adaptive decoupling is realized on a macroevolutionary scale in hemimetabolous insects and if the degree of adaptive decoupling is correlated with the strength of ontogenetic niche shifts. It is also unclear whether the degree of adaptive decoupling is correlated with phenotypic disparity. Here, we quantify nymphal and adult trait correlations in 219 species across the whole phylogeny of earwigs and stoneflies to test whether juvenile and adult traits are decoupled from each other. We demonstrate that adult head morphology is largely driven by nymphal ecology, and that adult head shape disparity has increased with stronger ontogenetic niche shifts in some stonefly lineages. Our findings implicate that the hemimetabolan metamorphosis in earwigs and stoneflies does not allow for high degrees of adaptive decoupling, and that high phenotypic disparity can even be realized when the evolution of distinct life phases is coupled.

Character Displacement Through an Evolutionary Change in Ontogenetic Niche Shifts

In communities structured by body size, coexistence can occur through combinations of ontogenetic changes in competitive ability and dietary niche. Using stable isotopes, we examined ontogenetic niche shifts in Trinidadian guppies (Poecilia reticulata) and killifish (Rivulus hartii) in three types of natural communities (both species with predators, KGP; both without predators, KG; killifish only, KO) and four experimental KG communities, initiated with KGP guppies and KO killifish between 13 and 45 years ago. In all communities, killifish occupied higher trophic positions and changed their diet (δ^13 C) with body size. Only KGP guppies displayed an ontogenetic niche shift. The KG guppies displayed a significant difference in trophic niche from KGP guppies, a character displacement that can facilitate coexistence with killifish. In the experimental communities, the guppy trophic niche was intermediate between those in KGP and KG communities, indicating that evolution has driven the niche shift in KG guppies.

Character Displacement Through and Evolutionary Change in Ontogenetic niche shifts

In communities structured by body size, coexistence can occur through combinations of ontogenetic changes in competitive ability and dietary niche. Using stable isotopes, we examined ontogenetic niche shifts in Trinidadian guppies (Poecilia reticulata) and killifish (Rivulus hartii) in three types of natural communities (both species with predators, KGP; both without predators, KG; killifish only, KO) and four experimental KG communities, initiated with KGP guppies and KO killifish between 13 and 45 years ago. In all communities, killifish occupied higher trophic positions and changed their diet (δ^13 C) with body size. Only KGP guppies displayed an ontogenetic niche shift. The KG guppies displayed a significant difference in trophic niche from KGP guppies, a character displacement that can facilitate coexistence with killifish. In the experimental communities, the guppy trophic niche was intermediate between those in KGP and KG communities, indicating that evolution has driven the niche shift in KG guppies.

Application of Combined Analyses of Stable Isotopes and Stomach Contents for Understanding Ontogenetic Niche Shifts in Silver Croaker (Pennahia argentata)

Stable isotope analysis (SIA) and stomach content analysis (SCA) were conducted to understand ontogenetic niche shifts in silver croaker Pennahia argentata inhabiting the southern coastal waters of the Korean peninsula. Sampled P. argentata were classified into three groups based on their total length (TL; 60–80 mm TL, 80–120 mm TL, and 120–210 mm TL). Carbon isotope (δ13C) ratios were distinguishable, whereas nitrogen isotope (δ15N) ratios were not significantly different among size classes, and Standard Ellipse Area (SEA), estimated by δ13C and δ15N, was expanded with increasing TL from 0.2 ‰2 (60–80 mm TL) to 2.0 ‰2 (120–210 mm TL). SCA results showed variable contribution of dietary items to each size class. In particular, higher dietary contribution of Polychaeta to P. argentata of 80–120 mm TL than 120–210 mm TL mirrored variation in δ13C values of P. argentata in those size classes. Based on the combined analyses involving SIA and SCA, we concluded that P. argentata underwent ontogenetic niche shifts, particularly dietary shifts, with growth stages. Ontogenetic niche shifting is a representative survival strategy in fish, and, therefore, represents essential information for managing fisheries. The present study demonstrated applicability of combined SIA and SCA analyses, not only for dietary resource tracing, but also for ecological niche studies.